利用表型性状构建杜仲雄性资源核心种质
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摘要
以306份杜仲雄性种质为试验材料,通过测量杜仲雄花和叶片等相关的22个表型性状,从遗传距离、取样方法、取样比例、聚类方法 4个层次探讨了构建杜仲雄性资源核心种质的最佳取样策略。应用均值差异百分率(MD)、方差差异百分率(VD)、极差符合率(CR)和变异系数变化率(VR)4个参数来检验各取样策略的优劣。通过比较核心种质和原始种质的表型多样性指数、符合率和主成分,对构建的核心种质进行代表性检验。结果表明:"多次聚类优先取样法+10%的取样比例+马氏距离+最短距离法"取样策略最佳,其均值差异百分率、方差差异百分率、极差符合率和变异系数变化率分别为0%、86.36%、100%和157.62%。核心种质的最终取样比例为10.8%。核心种质与原始种质22个表型性状上的多样性指数t检验结果不显著。核心种质与原始种质在22个指标上的均值符合率在92%~100%之间,最大值、最小值符合率为100%,多样性指数符合率在89%~100%之间。原始种质和核心种质的前10个主成分相同,累积贡献率分别为84.834%和90.422%。获得的33份杜仲雄性种质能够代表306份原始种质的表型变异特征。
306 male germplasms of Eucommia ulmoides were used as test materials.Through the measurement of22 related phenotypic traits,such as male flowers and leaves of E.ulmoides,we discussed the optimum sampling strategy to construct core germplasm resources of male E.ulmoides from four aspects of genetic distance,sampling method,sampling proportion and clustering method.The sampling strategies were tested by the value of MD,VD,CR and VR.By comparing the phenotypic diversity index,coincidence rate and principal component of the core and original germplasm,the core collection was tested by representativeness.The results showed that the optimal strategy was:multiple cluster priority sampling method +10% sampling proportion +mahalanobis distance +minimum distance method.The value of MD,VD,CR,VR were 0%,86.36%,100% and 157.62%,respectively.The final sampling proportion of core germplasm was 10.8%.The diversity index of 22 phenotypic characters of core germplasm and original germplasm was not significant by t test.The average coincidence rate between the core germplasm and the original germplasm on the 22 indexes was between 88.89%~100%,the maximum and minimum coincidence rate was 100%,and the diversity index coincidence rate was between 88.55%~98.54%.Thetop ten principal comp onents from the original germplasm and core germplasm were identical,and the accumulative contribution rates were 84.834% and 90.422%,respectively.The obtained male core collection of 33 E.ulmoide could satisfactorily represent the phenotypic variation characteristics of 306 E.ulmoide germplasms.
306 male germplasms of Eucommia ulmoides were used as test materials.Through the measurement of22 related phenotypic traits,such as male flowers and leaves of E.ulmoides,we discussed the optimum sampling strategy to construct core germplasm resources of male E.ulmoides from four aspects of genetic distance,sampling method,sampling proportion and clustering method.The sampling strategies were tested by the value of MD,VD,CR and VR.By comparing the phenotypic diversity index,coincidence rate and principal component of the core and original germplasm,the core collection was tested by representativeness.The results showed that the optimal strategy was:multiple cluster priority sampling method +10% sampling proportion +mahalanobis distance +minimum distance method.The value of MD,VD,CR,VR were 0%,86.36%,100% and 157.62%,respectively.The final sampling proportion of core germplasm was 10.8%.The diversity index of 22 phenotypic characters of core germplasm and original germplasm was not significant by t test.The average coincidence rate between the core germplasm and the original germplasm on the 22 indexes was between 88.89%~100%,the maximum and minimum coincidence rate was 100%,and the diversity index coincidence rate was between 88.55%~98.54%.Thetop ten principal comp onents from the original germplasm and core germplasm were identical,and the accumulative contribution rates were 84.834% and 90.422%,respectively.The obtained male core collection of 33 E.ulmoide could satisfactorily represent the phenotypic variation characteristics of 306 E.ulmoide germplasms.
引文
Diwan N.,Mcintosh M.S.,and Bauchan G.R.,1995,Methods of developing a core collection of annual Medicago species,Theor.Appl.Genet.,90(6):755-761
Dong Y.C.,Cao Y.S.,Zhang X.Y.,Liu S.C.,Wang L.F.,You G.X.,Pang B.S.,Li L.H.,and Jia J.Z.,2003,Establishment of candidate core collections in Chinese common wheat germplasm,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),4(1):1-8(董玉琛,曹永生,张学勇,刘三才,王兰芬,游光霞,庞斌双,李立会,贾继增,2003,中国普通小麦初选核心种质的产生,植物遗传资源学报,4(1):1-8)
Dong Y.S.,Zhuang B.C.,Zhao L.M.,Sun H.,Zhang M.,and He M.Y.,2000,The genetic diversity centers of annual wild soybean in China,Zuowu Xuebao(Acta Agronomica Sinica),26(5):521-527(董英山,庄炳昌,赵丽梅,孙寰,张明,何孟元,2000,中国野生大豆遗传多样性中心,作物学报,26(5):521-527)
Du H.Y.,ed.,2013,Report on development of China's Eucommia rubber resources and industry,Social Sciences Academic Press,Beijing,China,pp.20-27,41-47(杜红岩,编著,2013,中国杜仲橡胶资源与产业发展报告,社会科学文献出版社,中国,北京,pp.20-27,41-47)
Du H.Y.,ed.,2014,China Eucommia pictorial,China Forestry Publishing House,Beijing,China,pp.40-64(杜红岩,编著,2014,中国杜仲图志,中国林业出版社,中国,北京,pp.40-64)
Frankel O.H.,and Brown A.H.D.,1984,Plant genetic resources today:a critical appraisal,In:Holden J.H.W.,and Williams J.T.(eds),Crop Genetic Resources:Conservation and Evaluation,Allen and Unwin,London,UK,pp.249-257
Gao Z.H.,Zhang Z.,Han Z.H.,and Fang J.G.,2005,Development and evaluation of core collection of Japanese apricot germplasms in China,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),38(2):363-368(高志红,章镇,韩振海,房经贵,2005,中国果梅核心种质的构建与检测,中国农业科学,38(2):363-368)
Hu J.,Zhu J.,and Xu H.M.,2000,Methods of constructing core collections by stepwise clustering with three sampling strategies based on the genotypic values of crops,Theor.Appl.Genet.,101(1):264-268
Li X.L.,Jia J.W.,Wang J.H.,Ma W.J.,Ma J.W.,and Zhao Q.L.,2013,Morphological diversity analysis and preliminary construction of core collection of Catalpa fargesii Bureau,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),14(2):243-248(李秀兰,贾继文,王军辉,麻文俊,马建伟,赵秋玲,2013,灰楸形态多样性分析及核心种质初步构建,植物遗传资源学报,14(2):243-248)
Li Z.C.,Zhang H.L.,Cao Y.S.,Qiu Z.E.,Wei X.H.,Tang S.X.Yu P.,and Wang X.K.,2003,Studies on the sampling strategy for primary core collection of Chinese ingenious rice Zuowu Xuebao(Acta Agronomica Sinica),29(1):20-24(李自超,张洪亮,曹永生,裘宗恩,魏兴华,汤圣祥,余萍,王象坤,2003,中国地方稻种资源初级核心种质取样策略研究,作物学报,29(1):20-24)
Liu J.,Liao K.,Cao Q.,Sun Q.,Liu H.,and Jia Y.,2015 Establishment of wild apricot core collections based on phenotypic characters,Guoshu Xuebao(Journal of Fruit Science),32(5):787-796(刘娟,廖康,曹倩,孙琪,刘欢,贾杨,2015,利用表型性状构建新疆野杏种质资源核心种质,果树学报32(5):787-796)
Liu Z.C.,Zhang C.Y.,Zhang Y.M.,Zhang X.Y.,Wu C.J.,Wang H.B.,Shi J.,and Chen X.S.,2010,Study on method of constructing core collection of Malussieversii based on quantitative traits,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),43(2):358-370(刘遵春,张春雨,张艳敏,张小燕吴传金,王海波,石俊,陈学森,2010,利用数量性状构建新疆野苹果核心种质的方法,中国农业科学,43(2):358-370)
Maughan P.J.,Saghi Maroof M.A.,and Buss G.R.,1995,Microsatellite and amplified sequence length polymorphisms in cultivated and wild soybean,Genome,38(4):715-723
Ming J.,Zhang Q.X.,and Lan Y.P.,2005,Core collection of Prunus mume Seib.et Zucc,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),27(2):65-69(明军张启翔,兰彦平,2005,梅花品种资源核心种质构建,北京林业大学学报,27(2):65-69)
Van Hintum T.J.L.V.,and Haalman D.,1994,Pedigree analysis for composing a core collection of modern cultivars,with examples from barley(Hordeum vulgare,s.lat.),Theor.Appl.Genet.,88(1):70-74
Wang H.G.,Jia G.Q.,Zhi H.,Wen Q.F.,Dong J.L.,Chen L.Wang J.J.,Cao X.N.,Liu S.C.,Wang L.,Qiao Z.J.,and Diao X.M.,2016,Phenotypic diversity evaluations of foxtai millet core collections,Zuowu Xuebao(Acta Agronomica Sinica),42(1):19-30(王海岗,贾冠清,智慧,温琪汾,董俊丽陈凌,王君杰,曹晓宁,刘思辰,王纶,乔治军,刁现民,2016谷子核心种质表型遗传多样性分析及综合评价,作物学报,42(1):19-30)
Wang J.C.,Hu J.,Xu H.M.,and Zhang S.,2007,A strategy on constructing core collections by least distance stepwise sampling,Theor.Appl.Genet.,115(1):1-8
Wang J.C.,Hu J.,Zhang C.F.,Xu H.M.,and Zhang S.,2007,E-valuating parameters of rice core collections based on genotypic values and molecular marker information,Zhongguo Shuidao Kexue(Chinese Journal of Rice Science),21(1):51-58(王建成,胡晋,张彩芳,徐海明,张胜,2007,建立在基因型值和分子标记信息上的水稻核心种质评价参数,中国水稻科学,21(1):51-58)
Wang S.M.,and Zhang Z.W.,2011,The state of the world's plant genetic resources for food and agriculture,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),12(3):325-338(王述民,张宗文,2011,世界粮食和农业植物遗传资源保护与利用现状,植物遗传资源学报,12(3):325-338)
Xu H.M.,Hu J.,and Zhu J.,2000,An efficient method of sampling core collection from crop germplasm,Zuowu Xuebao(Acta Agronomica Sinica),26(2):157-162(徐海明,胡晋,朱军,2000,构建作物种质资源核心库的一种有效抽样方法,作物学报,26(2):157-162)
Xu H.M.,Li X.L.,Li J.Q.,Liu G.F.,and Hu J.,2005,Study on the strategies of integrating qualitative and quantitative traits for constructing core collection,Zhejiang Daxue Xuebao(Journal of Zhejiang University(Agriculture&Life Sciences)),31(4):362-367(徐海明,李晓玲,李金泉,刘桂富,胡晋,2005,整合质量数量性状构建作物核心种质的策略研究,浙江大学学报(农业与生命科学版),31(4):362-367)
Zhang Y.F.,2010,Development of core collection for Japanese persimmon from China based on morphological and SSRmakers,Dissertation for Ph.D.,Huazhong Agricultural University,Supervisor:Luo Z.R.,pp.8-24(张艳芳,2010,利用形态学和SSR标记建立柿核心种质,博士学位论文,华中农业大学,导师:罗正荣,pp.8-24)
Zhen g Y.Q.,Guo Y.,Fang S.J.,Xu Y.N.,Chen J.B.,and Liu J.X.,2014,Constructing pre-core collection of Cynodon dactylon based on phenotypic data,Caoye Xuebao(Acta Prataculturae Sinica),23(4):49-60(郑轶琦,郭琰,房淑娟,徐亚楠,陈静波,刘建秀,2014,利用表型数据构建狗牙根初级核心种质,草业学报,23(4):49-60)
Dong Y.C.,Cao Y.S.,Zhang X.Y.,Liu S.C.,Wang L.F.,You G.X.,Pang B.S.,Li L.H.,and Jia J.Z.,2003,Establishment of candidate core collections in Chinese common wheat germplasm,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),4(1):1-8(董玉琛,曹永生,张学勇,刘三才,王兰芬,游光霞,庞斌双,李立会,贾继增,2003,中国普通小麦初选核心种质的产生,植物遗传资源学报,4(1):1-8)
Dong Y.S.,Zhuang B.C.,Zhao L.M.,Sun H.,Zhang M.,and He M.Y.,2000,The genetic diversity centers of annual wild soybean in China,Zuowu Xuebao(Acta Agronomica Sinica),26(5):521-527(董英山,庄炳昌,赵丽梅,孙寰,张明,何孟元,2000,中国野生大豆遗传多样性中心,作物学报,26(5):521-527)
Du H.Y.,ed.,2013,Report on development of China's Eucommia rubber resources and industry,Social Sciences Academic Press,Beijing,China,pp.20-27,41-47(杜红岩,编著,2013,中国杜仲橡胶资源与产业发展报告,社会科学文献出版社,中国,北京,pp.20-27,41-47)
Du H.Y.,ed.,2014,China Eucommia pictorial,China Forestry Publishing House,Beijing,China,pp.40-64(杜红岩,编著,2014,中国杜仲图志,中国林业出版社,中国,北京,pp.40-64)
Frankel O.H.,and Brown A.H.D.,1984,Plant genetic resources today:a critical appraisal,In:Holden J.H.W.,and Williams J.T.(eds),Crop Genetic Resources:Conservation and Evaluation,Allen and Unwin,London,UK,pp.249-257
Gao Z.H.,Zhang Z.,Han Z.H.,and Fang J.G.,2005,Development and evaluation of core collection of Japanese apricot germplasms in China,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),38(2):363-368(高志红,章镇,韩振海,房经贵,2005,中国果梅核心种质的构建与检测,中国农业科学,38(2):363-368)
Hu J.,Zhu J.,and Xu H.M.,2000,Methods of constructing core collections by stepwise clustering with three sampling strategies based on the genotypic values of crops,Theor.Appl.Genet.,101(1):264-268
Li X.L.,Jia J.W.,Wang J.H.,Ma W.J.,Ma J.W.,and Zhao Q.L.,2013,Morphological diversity analysis and preliminary construction of core collection of Catalpa fargesii Bureau,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),14(2):243-248(李秀兰,贾继文,王军辉,麻文俊,马建伟,赵秋玲,2013,灰楸形态多样性分析及核心种质初步构建,植物遗传资源学报,14(2):243-248)
Li Z.C.,Zhang H.L.,Cao Y.S.,Qiu Z.E.,Wei X.H.,Tang S.X.Yu P.,and Wang X.K.,2003,Studies on the sampling strategy for primary core collection of Chinese ingenious rice Zuowu Xuebao(Acta Agronomica Sinica),29(1):20-24(李自超,张洪亮,曹永生,裘宗恩,魏兴华,汤圣祥,余萍,王象坤,2003,中国地方稻种资源初级核心种质取样策略研究,作物学报,29(1):20-24)
Liu J.,Liao K.,Cao Q.,Sun Q.,Liu H.,and Jia Y.,2015 Establishment of wild apricot core collections based on phenotypic characters,Guoshu Xuebao(Journal of Fruit Science),32(5):787-796(刘娟,廖康,曹倩,孙琪,刘欢,贾杨,2015,利用表型性状构建新疆野杏种质资源核心种质,果树学报32(5):787-796)
Liu Z.C.,Zhang C.Y.,Zhang Y.M.,Zhang X.Y.,Wu C.J.,Wang H.B.,Shi J.,and Chen X.S.,2010,Study on method of constructing core collection of Malussieversii based on quantitative traits,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),43(2):358-370(刘遵春,张春雨,张艳敏,张小燕吴传金,王海波,石俊,陈学森,2010,利用数量性状构建新疆野苹果核心种质的方法,中国农业科学,43(2):358-370)
Maughan P.J.,Saghi Maroof M.A.,and Buss G.R.,1995,Microsatellite and amplified sequence length polymorphisms in cultivated and wild soybean,Genome,38(4):715-723
Ming J.,Zhang Q.X.,and Lan Y.P.,2005,Core collection of Prunus mume Seib.et Zucc,Beijing Linye Daxue Xuebao(Journal of Beijing Forestry University),27(2):65-69(明军张启翔,兰彦平,2005,梅花品种资源核心种质构建,北京林业大学学报,27(2):65-69)
Van Hintum T.J.L.V.,and Haalman D.,1994,Pedigree analysis for composing a core collection of modern cultivars,with examples from barley(Hordeum vulgare,s.lat.),Theor.Appl.Genet.,88(1):70-74
Wang H.G.,Jia G.Q.,Zhi H.,Wen Q.F.,Dong J.L.,Chen L.Wang J.J.,Cao X.N.,Liu S.C.,Wang L.,Qiao Z.J.,and Diao X.M.,2016,Phenotypic diversity evaluations of foxtai millet core collections,Zuowu Xuebao(Acta Agronomica Sinica),42(1):19-30(王海岗,贾冠清,智慧,温琪汾,董俊丽陈凌,王君杰,曹晓宁,刘思辰,王纶,乔治军,刁现民,2016谷子核心种质表型遗传多样性分析及综合评价,作物学报,42(1):19-30)
Wang J.C.,Hu J.,Xu H.M.,and Zhang S.,2007,A strategy on constructing core collections by least distance stepwise sampling,Theor.Appl.Genet.,115(1):1-8
Wang J.C.,Hu J.,Zhang C.F.,Xu H.M.,and Zhang S.,2007,E-valuating parameters of rice core collections based on genotypic values and molecular marker information,Zhongguo Shuidao Kexue(Chinese Journal of Rice Science),21(1):51-58(王建成,胡晋,张彩芳,徐海明,张胜,2007,建立在基因型值和分子标记信息上的水稻核心种质评价参数,中国水稻科学,21(1):51-58)
Wang S.M.,and Zhang Z.W.,2011,The state of the world's plant genetic resources for food and agriculture,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),12(3):325-338(王述民,张宗文,2011,世界粮食和农业植物遗传资源保护与利用现状,植物遗传资源学报,12(3):325-338)
Xu H.M.,Hu J.,and Zhu J.,2000,An efficient method of sampling core collection from crop germplasm,Zuowu Xuebao(Acta Agronomica Sinica),26(2):157-162(徐海明,胡晋,朱军,2000,构建作物种质资源核心库的一种有效抽样方法,作物学报,26(2):157-162)
Xu H.M.,Li X.L.,Li J.Q.,Liu G.F.,and Hu J.,2005,Study on the strategies of integrating qualitative and quantitative traits for constructing core collection,Zhejiang Daxue Xuebao(Journal of Zhejiang University(Agriculture&Life Sciences)),31(4):362-367(徐海明,李晓玲,李金泉,刘桂富,胡晋,2005,整合质量数量性状构建作物核心种质的策略研究,浙江大学学报(农业与生命科学版),31(4):362-367)
Zhang Y.F.,2010,Development of core collection for Japanese persimmon from China based on morphological and SSRmakers,Dissertation for Ph.D.,Huazhong Agricultural University,Supervisor:Luo Z.R.,pp.8-24(张艳芳,2010,利用形态学和SSR标记建立柿核心种质,博士学位论文,华中农业大学,导师:罗正荣,pp.8-24)
Zhen g Y.Q.,Guo Y.,Fang S.J.,Xu Y.N.,Chen J.B.,and Liu J.X.,2014,Constructing pre-core collection of Cynodon dactylon based on phenotypic data,Caoye Xuebao(Acta Prataculturae Sinica),23(4):49-60(郑轶琦,郭琰,房淑娟,徐亚楠,陈静波,刘建秀,2014,利用表型数据构建狗牙根初级核心种质,草业学报,23(4):49-60)