干旱胁迫下外源茉莉酸甲酯对玉米幼苗根系吸水的影响
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摘要
茉莉酸类化合物作为环境信号分子,不仅参与植物生长发育的调控,同时受到环境胁迫的诱导,参与植物对逆境胁迫的响应和防御。本研究以北方广泛种植的玉米品种‘郑单958’为材料,通过对根系外源施加茉莉酸甲酯的方式,探究干旱胁迫下茉莉酸甲酯对玉米幼苗抗旱性以及根系吸水的影响。结果表明,外源茉莉酸甲酯可提高玉米幼苗光合速率、蒸腾作用和气孔导度,增强抗氧化酶活性,降低H_2O_2和丙二醛的含量,从而缓解干旱胁迫对植株造成的损伤。通过测定根系水导、氯化汞处理的蒸腾速率的变化以及水通道蛋白的表达量,发现干旱胁迫下外源茉莉酸甲酯可增强根系水通道蛋白的表达,进而增强玉米幼苗的根系吸水能力,从而缓解干旱胁迫造成的叶片水分含量的下降和水势的降低,提高了玉米幼苗的抗旱性。
Jasmonates,an environmental signal molecule,plays an important role not only in regulation of plant growth and development but also in plant response and defense to environmental stress.To investigate the effects of methyl jasmonate on drought tolerance and water absorption capacity of maize root at seedling stage,maize(Zea mays,‘Zhengdan 958’)seedlings,which are widely planted in the north China,were used as experimental material in this study.The results showed that exogenous methyl jasmonate could promote the maize seedling photosynthetic rate,stomatal conductance and transpiration rate,increase the antioxidant enzyme activity,reduce the contents of H_2O_(2 )and MDA,and then alleviate the damage caused by drought stress.By investigating the root hydraulic conductance,the changes of the whole plant transpiration rate caused by Hg Cl_(2 )and the expression level of aquaporin,it was supposed that exogenous methyl jasmonate could increase aquaporins expression of root under drought stress,and enhance the water absorption capacity of root,to alleviate the decrease of leaf water content and leaf water potential.These results indicated that exogenous methyl jasmonate could improve the drought tolerance of maize seedlings through enhancing the root water uptake.
Jasmonates,an environmental signal molecule,plays an important role not only in regulation of plant growth and development but also in plant response and defense to environmental stress.To investigate the effects of methyl jasmonate on drought tolerance and water absorption capacity of maize root at seedling stage,maize(Zea mays,‘Zhengdan 958’)seedlings,which are widely planted in the north China,were used as experimental material in this study.The results showed that exogenous methyl jasmonate could promote the maize seedling photosynthetic rate,stomatal conductance and transpiration rate,increase the antioxidant enzyme activity,reduce the contents of H_2O_(2 )and MDA,and then alleviate the damage caused by drought stress.By investigating the root hydraulic conductance,the changes of the whole plant transpiration rate caused by Hg Cl_(2 )and the expression level of aquaporin,it was supposed that exogenous methyl jasmonate could increase aquaporins expression of root under drought stress,and enhance the water absorption capacity of root,to alleviate the decrease of leaf water content and leaf water potential.These results indicated that exogenous methyl jasmonate could improve the drought tolerance of maize seedlings through enhancing the root water uptake.
引文
Anjum SA,Wang L,Farooq M,et al(2011a).Methyl jasmonate-induced alteration in lipid peroxidation,antioxidative defence system and yield in soybean under drought.JAgron Crop Sci,197:296-301
Anjum SA,Xie XY,Farooq M,et al(2011b).Effect of exogenous methyl jasmonate on growth,gas exchange and chlorophyll contents of soybean subjected to drought.Afr J Biotechnol,10:9640-9646
Beauchamp CO,Fridovich I(1973).Isozymes of superoxide dismutase from wheat germ.Biochim Biophys Acta,317:50-64
Cai KZ,Dong TX,Xu T(2006).The physiological roles and resistance control in stress environment of jasmonates.Ecol Environ,15:397-404(in Chinese with English abstract)[蔡昆争,董桃杏,徐涛(2006).茉莉酸类物质(JAs)的生理特性及其在逆境胁迫中的抗性作用.生态环境学报,15:397-404]
Cheong JJ,Yang DC(2003).Methyl jasmonate as a vital substance in plants.Trends Genet,19:409
Dong TX,Cai KZ,Zeng RS(2009).Effect of methyl jasmonate(Me JA)on photosynthetic traits of rice seedlings under drought stress.Ecol Environ,18:1872-1876(in Chinese with English abstract)[董桃杏,蔡昆争,曾任森(2009).茉莉酸甲酯(Me JA)对干旱胁迫下水稻幼苗光合作用特性的影响.生态环境学报,18:1872-1876]
Du CY,Duan ZY,Pan YH,et al(2015).Effect of drought stress on growth and activities of antioxidant enzymes of maize seedling.Agric Res Arid Area,33:124-129(in Chinese with English abstract)[杜彩艳,段宗颜,潘艳华等(2015).干旱胁迫对玉米苗期植株生长和保护酶活性的影响.干旱地区农业研究,33:124-129]
Hamurcu M,Sekmen AH,Turkan I,et al(2013).Induced anti-oxidant activity in soybean alleviates oxidative stress under moderate boron toxicity.Plant Growth Regul,70:217-226
Heath RL,Packer L(1968).Photoperoxidation in isolated chloropiasts.I,kinetics and stoichiometry of fatty acid peroxidantion.Arch Biochem Biophys,125:189-198
Javot H,Maurel C(2002).The role of aquaporins in root water uptake.Ann Bot,90:301-313
Kochba J,Lavee S,Spiegel-Roy P(1977).Differences in peroxidase activity and isoenzymes in embryogenic ane non-embryogenic‘Shamouti’orange ovular callus lines.Plant Cell Physiol,18:463-467
Li MS,Yan XF(2014).Jasmonic acid signaling in plants and its biological functions in relation to environment.Acta Ecol Sin,34:6779-6788(in Chinese with English abstract)[李梦莎,阎秀峰(2014).植物的环境信号分子茉莉酸及其生物学功能.生态学报,34:6779-6788]
Liu P(2014).The mechanism of silicon on enhancing sorghum resistance to drought,salt,cadmium,K-deficiency(dissertation).Yangling,Shaanxi:Research Center of Soil and Water Conservation and Ecological Environment,Chinese Academy of Sciences and Ministry of Education(in Chinese with English abstract)[刘朋(2014).硅增强高粱抗干旱、盐和镉胁迫能力以及缓解钾缺乏的作用机制研究(学位论文).陕西杨凌:中国科学院教育部水土保持与生态环境研究中心]
Ma C,Wang ZQ,Zhang LT,et al(2014).Photosynthetic responses of wheat(Triticum aestivum L.)to combined effects of drought and exogenous methyl jasmonate.Phosyntheticaot,52:377-385
Machado S,Paulsen GM(2001).Combined effects of drought and high temperature on water relations of wheat and sorghum.Plant Soil,233:179-187
Miyamoto N,Steudle E,Hirasawa T,et al(2001).Hydraulic conductivity of rice roots.J Exp Bot,52:1835-1846
Mu ZX,Zhang SQ,Liang AH,et al(2005).Relationship between maize root hydraulic conductivity and drought resistance.Acta Agron Sin,31:203-208(in Chinese with English abstract)[慕自新,张岁岐,梁爱华等(2005).玉米整株根系水导与其表型抗旱性的关系.作物学报,31:203-208]
Nakano Y,Asada K(1981).Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts.Plant Cell Physiol,22:867-880
Pan RC,Gu HQ(1995).Effect of methyl jasmonate on the growth and drought resistance in peanut seedlings.Acta Phytophysiol Sin,21:215-220(in Chinese with English abstract)[潘瑞炽,古焕庆(1995).茉莉酸甲酯对花生幼苗生长和抗旱性的影响.植物生理学报,21:215-220]
Sutka M,Li G,Boudet J,et al(2011).Natural variation of root hydraulics in arabidopsis grown in normal and saltstressed conditions.Plant Physiol,155:1264-1276
Velikova V,Yordanov I,Edreva A(2000).Oxidative stress and some antioxidant systems in acid rain-treated bean plants:protective role of exogenous polyamines.Plant Sci,151:59-66
Wasternack C(2014).Action of jasmonates in plant stress responses and development-applied aspects.Biotechnol Adv,32:31-39
Wu H,Wu X,Li Z,et al(2012).Physiological evaluation of drought stress tolerance and recovery in cauliflower(Brassica oleracea L.)seedlings treated with methyl jasmonate and coronatine.J Plant Growth Regul,31:113-123
Yang Y,Chang D,Wang Y,et al(2016).Effect of methyl jasmonate(Me JA)on enhancing drought resistance of cotton.Acta Agri Boreal-Occident Sin,25:1333-1341(in Chinese with English abstract)[杨艺,常丹,王艳等(2016).茉莉酸甲酯对棉花抗旱效果的影响.西北农业学报,25:1333-1341]
Zhang SQ,Shan L(2001).Research progress on water uptake in plant roots.Chin J Appl Environ Biol,7:396-402(in Chinese with English abstract)[张岁岐,山仑(2001).根系吸水机理研究进展.应用与环境生物学报,7:396-402]
Anjum SA,Xie XY,Farooq M,et al(2011b).Effect of exogenous methyl jasmonate on growth,gas exchange and chlorophyll contents of soybean subjected to drought.Afr J Biotechnol,10:9640-9646
Beauchamp CO,Fridovich I(1973).Isozymes of superoxide dismutase from wheat germ.Biochim Biophys Acta,317:50-64
Cai KZ,Dong TX,Xu T(2006).The physiological roles and resistance control in stress environment of jasmonates.Ecol Environ,15:397-404(in Chinese with English abstract)[蔡昆争,董桃杏,徐涛(2006).茉莉酸类物质(JAs)的生理特性及其在逆境胁迫中的抗性作用.生态环境学报,15:397-404]
Cheong JJ,Yang DC(2003).Methyl jasmonate as a vital substance in plants.Trends Genet,19:409
Dong TX,Cai KZ,Zeng RS(2009).Effect of methyl jasmonate(Me JA)on photosynthetic traits of rice seedlings under drought stress.Ecol Environ,18:1872-1876(in Chinese with English abstract)[董桃杏,蔡昆争,曾任森(2009).茉莉酸甲酯(Me JA)对干旱胁迫下水稻幼苗光合作用特性的影响.生态环境学报,18:1872-1876]
Du CY,Duan ZY,Pan YH,et al(2015).Effect of drought stress on growth and activities of antioxidant enzymes of maize seedling.Agric Res Arid Area,33:124-129(in Chinese with English abstract)[杜彩艳,段宗颜,潘艳华等(2015).干旱胁迫对玉米苗期植株生长和保护酶活性的影响.干旱地区农业研究,33:124-129]
Hamurcu M,Sekmen AH,Turkan I,et al(2013).Induced anti-oxidant activity in soybean alleviates oxidative stress under moderate boron toxicity.Plant Growth Regul,70:217-226
Heath RL,Packer L(1968).Photoperoxidation in isolated chloropiasts.I,kinetics and stoichiometry of fatty acid peroxidantion.Arch Biochem Biophys,125:189-198
Javot H,Maurel C(2002).The role of aquaporins in root water uptake.Ann Bot,90:301-313
Kochba J,Lavee S,Spiegel-Roy P(1977).Differences in peroxidase activity and isoenzymes in embryogenic ane non-embryogenic‘Shamouti’orange ovular callus lines.Plant Cell Physiol,18:463-467
Li MS,Yan XF(2014).Jasmonic acid signaling in plants and its biological functions in relation to environment.Acta Ecol Sin,34:6779-6788(in Chinese with English abstract)[李梦莎,阎秀峰(2014).植物的环境信号分子茉莉酸及其生物学功能.生态学报,34:6779-6788]
Liu P(2014).The mechanism of silicon on enhancing sorghum resistance to drought,salt,cadmium,K-deficiency(dissertation).Yangling,Shaanxi:Research Center of Soil and Water Conservation and Ecological Environment,Chinese Academy of Sciences and Ministry of Education(in Chinese with English abstract)[刘朋(2014).硅增强高粱抗干旱、盐和镉胁迫能力以及缓解钾缺乏的作用机制研究(学位论文).陕西杨凌:中国科学院教育部水土保持与生态环境研究中心]
Ma C,Wang ZQ,Zhang LT,et al(2014).Photosynthetic responses of wheat(Triticum aestivum L.)to combined effects of drought and exogenous methyl jasmonate.Phosyntheticaot,52:377-385
Machado S,Paulsen GM(2001).Combined effects of drought and high temperature on water relations of wheat and sorghum.Plant Soil,233:179-187
Miyamoto N,Steudle E,Hirasawa T,et al(2001).Hydraulic conductivity of rice roots.J Exp Bot,52:1835-1846
Mu ZX,Zhang SQ,Liang AH,et al(2005).Relationship between maize root hydraulic conductivity and drought resistance.Acta Agron Sin,31:203-208(in Chinese with English abstract)[慕自新,张岁岐,梁爱华等(2005).玉米整株根系水导与其表型抗旱性的关系.作物学报,31:203-208]
Nakano Y,Asada K(1981).Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts.Plant Cell Physiol,22:867-880
Pan RC,Gu HQ(1995).Effect of methyl jasmonate on the growth and drought resistance in peanut seedlings.Acta Phytophysiol Sin,21:215-220(in Chinese with English abstract)[潘瑞炽,古焕庆(1995).茉莉酸甲酯对花生幼苗生长和抗旱性的影响.植物生理学报,21:215-220]
Sutka M,Li G,Boudet J,et al(2011).Natural variation of root hydraulics in arabidopsis grown in normal and saltstressed conditions.Plant Physiol,155:1264-1276
Velikova V,Yordanov I,Edreva A(2000).Oxidative stress and some antioxidant systems in acid rain-treated bean plants:protective role of exogenous polyamines.Plant Sci,151:59-66
Wasternack C(2014).Action of jasmonates in plant stress responses and development-applied aspects.Biotechnol Adv,32:31-39
Wu H,Wu X,Li Z,et al(2012).Physiological evaluation of drought stress tolerance and recovery in cauliflower(Brassica oleracea L.)seedlings treated with methyl jasmonate and coronatine.J Plant Growth Regul,31:113-123
Yang Y,Chang D,Wang Y,et al(2016).Effect of methyl jasmonate(Me JA)on enhancing drought resistance of cotton.Acta Agri Boreal-Occident Sin,25:1333-1341(in Chinese with English abstract)[杨艺,常丹,王艳等(2016).茉莉酸甲酯对棉花抗旱效果的影响.西北农业学报,25:1333-1341]
Zhang SQ,Shan L(2001).Research progress on water uptake in plant roots.Chin J Appl Environ Biol,7:396-402(in Chinese with English abstract)[张岁岐,山仑(2001).根系吸水机理研究进展.应用与环境生物学报,7:396-402]