海洋枝顶孢霉BH0531发酵液对根结线虫卵及二龄幼虫的影响
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摘要
实验以根结线虫的卵、卵囊和二龄幼虫(J2)为研究对象,对海洋枝顶孢霉(Acremonium sp.)BH0531发酵液处理根结线虫不同发育阶段的杀线作用进行了效果评价,并以J2幼虫为靶标,与市面上的灭线磷及阿维菌素的药效进行了初步对比。结果显示,在实验条件下,海洋枝顶孢霉BH0531发酵液对根结线虫卵囊和分散卵的孵化以及J2幼虫的活力均具有明显抑制作用,其对卵囊孵化的抑制作用达40.18%;对分散卵孵化的抑制作用可达84.28%;而对J2幼虫的杀线作用最为显著,校正死亡率高达97.41%,高于实验条件下灭线磷及阿维菌素的测定值,在根结线虫的生物防控方面显现出潜在的研究价值。
Using the eggs, egg masses and 2~(nd) stage juveniles(J2) of root-knot nematodes as research object, the nematicidal effect of fermentation broth of marine-derived Acremonium sp. BH0531 on root-knot nematodes of different development stages was evaluated. With the J2 as target, the pesticide effect of fermentation broth was compared with ethoprophos and abamectin sold on the market. The results showed that under the experimental conditions, the fermentation broth of marine-derived Acremonium sp. BH0531 all had obvious inhibitory effect on the vitality of egg masses,individual eggs hatching and J2, and the inhibiting effect of fermentation broth on egg masses, individual eggs hatching were 40.18% and 84.28%,respectively. The nematicidal effect on J2 was the most significant, the corrected death rate was up to 97.41%, which was higher than that of ethoprophos and abamectin under the experimental conditions. These results suggested that the fermentation broth of marine-derived Acremonium sp.BH0531 had its potential value in the bio-control of root-knot nematode.
Using the eggs, egg masses and 2~(nd) stage juveniles(J2) of root-knot nematodes as research object, the nematicidal effect of fermentation broth of marine-derived Acremonium sp. BH0531 on root-knot nematodes of different development stages was evaluated. With the J2 as target, the pesticide effect of fermentation broth was compared with ethoprophos and abamectin sold on the market. The results showed that under the experimental conditions, the fermentation broth of marine-derived Acremonium sp. BH0531 all had obvious inhibitory effect on the vitality of egg masses,individual eggs hatching and J2, and the inhibiting effect of fermentation broth on egg masses, individual eggs hatching were 40.18% and 84.28%,respectively. The nematicidal effect on J2 was the most significant, the corrected death rate was up to 97.41%, which was higher than that of ethoprophos and abamectin under the experimental conditions. These results suggested that the fermentation broth of marine-derived Acremonium sp.BH0531 had its potential value in the bio-control of root-knot nematode.
引文
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[3]蔡加星.番茄南方根结线虫防控技术的研究[D].杭州:浙江大学,2016.
[4]刘维志.植物病原线虫学[M].北京:中国农业出版社,2000:1-10.
[5]郝祥国.根结线虫病防治措施[J].宁夏农林科技,2013,54(2):125-126.
[6]MENG Q H,SHI X X.Isolation of an Acremnium sp.from a screening of52 seawater fungal isolates and preliminary characterization of its growth conditions and nematicidal activity[J].Biotechnol Lett,2012,34(10):1847-1850.
[7]黄敏,刘晓霞,申佩娟.海洋枝顶孢霉BH0531对黄瓜根结线虫防治的微生态效应[J].中国酿造,2016,35(3):52-56.
[8]蔡爽,申佩娟,刘晓霞.海洋真菌BH0531对黄瓜促生长作用的研究[J].中国酿造,2016,35(3):27-31.
[9]蔡爽,刘晓霞,申佩娟.海洋真菌BH0531代谢产物对感染根结线虫黄瓜保护酶的影响[J].中国瓜菜,2016,29(7):9-13.
[10]杜海霞,蒙春蕾,王勇勇.海洋枝顶孢霉BH0531代谢物对松材线虫形态和酶活的影响[J].中国酿造,2014,33(6):23-26.
[11]BIESMEIJER J C,ROBERTS S P M,REEMER M,et al.Parallel declines in pollinators and insect-pollinated plants in Britain and the Netherlands[J].Science,2006,313(5785):351-354.
[12]GIANNAKOU I O,KARPOUZAS D G,ANASTASIADES I,et al.Factors affecting the efficacy of non-fumigant nematicides for controlling root-knot nematodes[J].Pest Manage Sci,2005,61(10):961-972.
[13]张瑞峰,魏亚东,王金成.不同杀线剂对穿刺短体线虫的杀灭效果研究[J].植物检疫,2012,26(2):5-8.
[14]朱卫刚,胡伟群,陈定花.阿维菌素对南方根结线虫的生物活性[J].现代农药,2008,7(4):38-39,48.
[15]漆永红,曹素芳,吕和平,等.不同药剂对南方根结线虫卵孵化及2龄幼虫活性的影响[J].西北农业学报,2011,20(9):184-189.
[16]王波.淡紫拟青霉对南方根结线虫卵孵化的影响及其与放线菌代谢物复配对南方根结线虫生物防治的研究[D].南京:南京农业大学,2009.