4个栽培年限丹参颜色与9种活性成分含量的相关性
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摘要
目的研究4个栽培年限(1、2、3、5年)丹参Salvia miltiorrhiza Bge.颜色与9种活性成分含有量的相关性。方法精密色差仪测定色度值(L*、a*、b*),HPLC法测定水溶性成分(紫草酸、丹酚酸B、迷迭香酸、原儿茶醛酸、丹参素钠)和脂溶性成分(二氢丹参酮Ⅰ、丹参酮ⅡA、隐丹参酮、丹参酮Ⅰ)含有量,通过Pearson相关性分析考察颜色与各成分含有量的相关性。结果 2年生丹参的L*、b*值最高,3年生者a*值最高,5年生者各色度值均最低。各成分含有量均在3年生者中达到最高。紫草酸、丹参素钠与各色度值均无显著相关性,丹酚酸B、迷迭香酸、丹参酮ⅡA与其均呈显著正相关,原儿茶醛酸与其均呈显著负相关。结论栽培年限为3年的丹参质量较高。
AIM To study the correlations between colors and contents of nine active constituents of Salvia miltiorrhiza Bge. with four growth years( one,two,three and five years). METHODS The chromatic values( L*,a*and b*) were determined by precise color difference meter. HPLC was applied to determining the contents of water-soluble constituents( lithospermic acid,salvianolic acid B,rosmarinic acid,protocatechuic aldehyde acid and salvianic acid A sodium) and liposoluble-soluble constituents( dihydrotanshinoneⅠ,cryptotanshinone,tanshinone Ⅰand tanshinone ⅡA). Then the correlations between colors and contents of various constituents were investigated by Pearson correlation analysis. RESULTS L*and b*values of two growth-year S. miltiorrhiza,together with a*value of three growth-year S. miltiorrhiza,were the highest,but various chromatic values of five growth-year S. miltiorrhiza were all the lowest. The contents of various constituents in three growth-year S. miltiorrhiza reached the highest. Lithospermic acid and salvianic acid A sodium showed no significant correlations with various chromatic values,salvianolic B,rosmarinic acid and tanshinone ⅡA exhibited significantly positive correlations with them,and protocatechuic aldehyde had significantly negative correlations with them. CONCLUSION The quality of three growth-year S. miltiorrhiza is relatively high.
AIM To study the correlations between colors and contents of nine active constituents of Salvia miltiorrhiza Bge. with four growth years( one,two,three and five years). METHODS The chromatic values( L*,a*and b*) were determined by precise color difference meter. HPLC was applied to determining the contents of water-soluble constituents( lithospermic acid,salvianolic acid B,rosmarinic acid,protocatechuic aldehyde acid and salvianic acid A sodium) and liposoluble-soluble constituents( dihydrotanshinoneⅠ,cryptotanshinone,tanshinone Ⅰand tanshinone ⅡA). Then the correlations between colors and contents of various constituents were investigated by Pearson correlation analysis. RESULTS L*and b*values of two growth-year S. miltiorrhiza,together with a*value of three growth-year S. miltiorrhiza,were the highest,but various chromatic values of five growth-year S. miltiorrhiza were all the lowest. The contents of various constituents in three growth-year S. miltiorrhiza reached the highest. Lithospermic acid and salvianic acid A sodium showed no significant correlations with various chromatic values,salvianolic B,rosmarinic acid and tanshinone ⅡA exhibited significantly positive correlations with them,and protocatechuic aldehyde had significantly negative correlations with them. CONCLUSION The quality of three growth-year S. miltiorrhiza is relatively high.
引文
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[10]王涛,贺帆,詹军,等.烘烤过程中不同部位烟叶颜色值和主要化学成分的变化[J].湖南农业大学学报:自然科学版,2012,38(2):125-130.
[11]林蔚兰,邓乔华,卢绵,等.三个丹参主产区生产情况调查[J].中药材,2008,31(3):338-340.
[2]肖培根,赵润怀,龙兴超,等.中药资源可持续发展产销情况的宏观分析[J].中国中药杂志,2009,34(17):2135-2139.
[3]刘伟,张琳,章云云,等.不同连作年限对白花丹参生长及其活性成分含量的影响[J].中国中药杂志,2013,38(24):4252-4256.
[4]Gao Z Q,Zhang S X.Continuous cropping obstacle and rhizospheric microecologyⅡ.Root exudates and their ecological effects[J].Chin J Appl Ecol,1998,9(5):549-554.
[5]张辰露,孙群,叶青.连作对丹参生长的障碍效应[J].西北植物学报,2005,25(5):1029-1034.
[6]李先恩,张晓阳.连作对丹参产量和质量的影响及施肥的作用[J].世界中医药,2015,10(8):1148-1151.
[7]张永清,刘合刚.药用植物栽培学[M].9版.北京:中国中医药出版社,2013.
[8]尉广飞,李翠,刘谦,等.丹参根部直径与其活性成分含有量间的相关性研究[J].中成药,2015,37(8):1781-1785.
[9]尉广飞,李佳,刘谦,等.丹参根部颜色及其与活性成分含量的相关性研究[J].山东农业科学,2015,47(8):59-62.
[10]王涛,贺帆,詹军,等.烘烤过程中不同部位烟叶颜色值和主要化学成分的变化[J].湖南农业大学学报:自然科学版,2012,38(2):125-130.
[11]林蔚兰,邓乔华,卢绵,等.三个丹参主产区生产情况调查[J].中药材,2008,31(3):338-340.