智托洁白丸对低氧应激性胃溃疡的保护作用
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摘要
本研究为了探讨智托洁白丸(ZJP)对模拟海拔5 000 m的高原环境所诱发的大鼠低氧应激性胃溃疡的影响,以ZJP的药效与机制作为研究对象,通过组织病理形态学和透射电镜观察、腺苷酸含量和能荷测定、SOD活性和MDA含量测定的方法,发现ZJP由于增加大鼠胃粘膜的ATP、ADP、AMP含量及能荷、增加SOD活性,降低MDA含量而减轻了低氧应激所导致的胃粘膜的溃疡损伤,表明ZJP具有保护低氧应激性胃溃疡的药理作用。我们的研究有助于为高原病的防治提供药学理论依据。
The objective of this study was to explore the effect of Zhituo Jiebai Pills(ZJP) on rats with hypoxic gastric ulcer induced by simulated altitude of 5 000 m. The pharmacodynamics and mechanism of ZJP were focused as objects of this research. By means of histopathologic morphology and ultrastructural changes, content of adenosine and energy charge determination, activity of SOD and content of MDA determination, we found that the ZJP could alleviate gastric mucosal ulcer damage caused by hypoxic stress by increasing ATP, ADP, AMP content and energy charge, increasing SOD activity and reducing MDA content in gastric mucosa of rats, which indicated that ZJP had the pharmacological action of protecting gastric ulcer caused by hypoxic stress. This research would be helpful to provide the pharmacological basis for the prevention and treatment of altitude diseases.
The objective of this study was to explore the effect of Zhituo Jiebai Pills(ZJP) on rats with hypoxic gastric ulcer induced by simulated altitude of 5 000 m. The pharmacodynamics and mechanism of ZJP were focused as objects of this research. By means of histopathologic morphology and ultrastructural changes, content of adenosine and energy charge determination, activity of SOD and content of MDA determination, we found that the ZJP could alleviate gastric mucosal ulcer damage caused by hypoxic stress by increasing ATP, ADP, AMP content and energy charge, increasing SOD activity and reducing MDA content in gastric mucosa of rats, which indicated that ZJP had the pharmacological action of protecting gastric ulcer caused by hypoxic stress. This research would be helpful to provide the pharmacological basis for the prevention and treatment of altitude diseases.
引文
Ali T.,and Harty R.F.,2009,Stress-induced ulcer bleeding in critically ill patients,Gastroenterol.Clin.North Am.,38(2):245-265
Cheng Y.T.,Lu C.C.,and Yen G.C.,2015,Beneficial effects of camellia oil(Camellia Oleifera abel.)on hepatoprotective and gastroprotective activities,J.Nutr.Sci.Vitaminol.,61:S100-S102
Feng B.,Liu Z.,Xing Y.W.,Gao A.,Zhu H.M.,and Wang J.,2013,Advance in studies on effect of traditional Chinese(Tibetan)medicines in prevention and treatment of acute altitude sickness,China Journal of Chinese Materia Medica,38(12):1876-1880
Kamyab A.A.,and Eshraghian A.,2013,Anti-Inflammatory,gastrointestinal and hepatoprotective effects of Ocimum sanctum Linn:an ancient remedy with new application,Inflamm.Allergy Drug Targets,12(6):378-384
Li Y.H.,and Yang M.,2017,Establishment of rat model of plateau hypoxia caused stress ulcer,Chongqing Yixue(Chongqing Medicine),(27):3825-3827(李永慧,杨梅,2017,高原低氧致应激性溃疡大鼠模型的建立,重庆医学,(27):3825-3827)
Parra guez V.H.,Urquieta B.,Perez L.,Castellaro G.,De los Reyes M.,Torres-Rovira L.,Aguado-Martínez A.,Astiz S.,and González-Bulnes A.,2013,Fertility in a high-altitude environment is compromised by luteal dysfunction:the relative roles of hypoxia and oxidative stress,Reprod.Bio.Endocrinol.,11(1):1-12
Repetto M.G.,and Ll esuy S.F.,2002,Antioxidant properties of natural compounds used in popular medicine for gastric ulcers,Braz.J.Med.Biol.Res.,35(5):523-534
Sahin S.,Ayar G.,Yazici M.U.,Koksal T.,Akman A.O.,Gunduz R.C.,Kirsacoglu C.T.,and Gulerman F.,2016,Stress induced gastrointestinal bleeding in a pediatric intensive care unit:which risk factors should necessitate prophilaxis?Minerva Pediatr.,68(1):19-26
Shao K.,Shan T.D.,and Zhang F.X.,2011,Progress in study on gastrointestinal stress ulcer caused by high altitude hypoxia,Weichangbing Xue(Chinese Journal of Gastroenterology),(1):57-59(邵珂,单体栋,张方信,2011,高原低氧致胃肠应激性溃疡的研究进展,胃肠病学,(1):57-59)
Sijacki A.D.,Popovic N.,Karamarkovic A.,Stefanovic B.D.,Djukic V.,Lausevic Z.,Krstic S.N.,Bumbasirevic V.,Gregoric P.D.,Bajec D.J.D.,and Jeremic V.,2007,Risk factors for bleeding from stress ulcer in severely injured and critically ill persons,Acta Chir.Iugosl.,54(1):77-81
Silen W.,1988,Experimental models of gastric ulceration and injury,Am.J.Physiol.,255(1):395-402
Subbotina L.A.,2008,Stress effect on the blood aggregate state,Aviakosm.Ekolog.Med.,42(3):34-37
Syam A.F.,Simadibrata M.,Wanandi S.I.,Hernowo B.S.,Sadikin M.,and Rani A.A.,2011,Gastric ulcers induced by systemic hypoxia,Acta Med.Indones.,43(4):243-248
Tafti L.D.,Shariatpanahi S.M.,Damghani M.M.,and Javadi B.,2017,Tradition al persian topical medications for gastrointestinal diseases,Iran.J.Basic Med.Sci.,20(3):222-241
Wu T.Y.,Ding S.Q.,Liu J.L.,Jia J.H.,Chai Z.C.,and Dai R.C.,201 2,Who are more at risk for acute mountain sickness:a prospective study in Qinghai-Tibet railroad construction workers on Mt.Tanggula.,Chin.Med.J.,125(8):1393-1400
Wu T.Y.,Ding S.Q.,Liu J.L.,Jia J.H.,Dai R.C.,Zhu D.C.,Liang B.Z.,Qi D.T.,and Sun Y.F.,2007,High-altitude gastrointestinal bleeding:an observation in Qinghai-Tibetan railroad construction workers on Mountain Tanggula,World J.Gastroenterol.,13(5):774-780
Wu T.Y.,Ding S.Q.,Liu J.L.,Yu M.T.,Jia J.H.,Chai Z.C.,Dai R.C.,Zhang S.L.,Li B.Y.,Pan L.,Liang B.Z.,Zhao J.Z.,Qi de T.,Sun Y.F.,and Kayser B.,2007,Who should not go high:chronic disease and work at altitude during construction of the Qinghai-Tibet railroad,High Alt.Med.Biol.,8(2):88-107
Wu T.Y.,Ding S.Q.,Liu J.L.,Yu M.T.,Jia J.H.,Duan J.Q.,Chai Z.C.,Dai R.C.,Zhang S.L.,Liang B.Z.,Zhao J.Z.,Qi de T.,Sun Y.F.,and Kayser B.,2009,Reduced incidence and severity of acute mountain sickness in Qinghai-Tibet railroad construction workers after repeated 7-month exposures despite 5-month low altitude periods,High Alt.Med.Biol.,10(3):221-232
Yung H.W.,Cox M.,Tissot van Patot M.,and Burton G.J.,2012,Evidence of endoplasmic reticulum stress and protein synthesis inhibition in the placenta of non-native women at high altitude,FASEB J.,26(5):1970-1981
Zhao Y.,Luo C.,Lan X.M.,Liu Y.,and Sun Y.Z.,2016,Mechanism of Zhituo jiebai pills on gastric ulcer induced by acetic acid in mice,Journal of Jiangxi University of TCM,28:65-67
Cheng Y.T.,Lu C.C.,and Yen G.C.,2015,Beneficial effects of camellia oil(Camellia Oleifera abel.)on hepatoprotective and gastroprotective activities,J.Nutr.Sci.Vitaminol.,61:S100-S102
Feng B.,Liu Z.,Xing Y.W.,Gao A.,Zhu H.M.,and Wang J.,2013,Advance in studies on effect of traditional Chinese(Tibetan)medicines in prevention and treatment of acute altitude sickness,China Journal of Chinese Materia Medica,38(12):1876-1880
Kamyab A.A.,and Eshraghian A.,2013,Anti-Inflammatory,gastrointestinal and hepatoprotective effects of Ocimum sanctum Linn:an ancient remedy with new application,Inflamm.Allergy Drug Targets,12(6):378-384
Li Y.H.,and Yang M.,2017,Establishment of rat model of plateau hypoxia caused stress ulcer,Chongqing Yixue(Chongqing Medicine),(27):3825-3827(李永慧,杨梅,2017,高原低氧致应激性溃疡大鼠模型的建立,重庆医学,(27):3825-3827)
Parra guez V.H.,Urquieta B.,Perez L.,Castellaro G.,De los Reyes M.,Torres-Rovira L.,Aguado-Martínez A.,Astiz S.,and González-Bulnes A.,2013,Fertility in a high-altitude environment is compromised by luteal dysfunction:the relative roles of hypoxia and oxidative stress,Reprod.Bio.Endocrinol.,11(1):1-12
Repetto M.G.,and Ll esuy S.F.,2002,Antioxidant properties of natural compounds used in popular medicine for gastric ulcers,Braz.J.Med.Biol.Res.,35(5):523-534
Sahin S.,Ayar G.,Yazici M.U.,Koksal T.,Akman A.O.,Gunduz R.C.,Kirsacoglu C.T.,and Gulerman F.,2016,Stress induced gastrointestinal bleeding in a pediatric intensive care unit:which risk factors should necessitate prophilaxis?Minerva Pediatr.,68(1):19-26
Shao K.,Shan T.D.,and Zhang F.X.,2011,Progress in study on gastrointestinal stress ulcer caused by high altitude hypoxia,Weichangbing Xue(Chinese Journal of Gastroenterology),(1):57-59(邵珂,单体栋,张方信,2011,高原低氧致胃肠应激性溃疡的研究进展,胃肠病学,(1):57-59)
Sijacki A.D.,Popovic N.,Karamarkovic A.,Stefanovic B.D.,Djukic V.,Lausevic Z.,Krstic S.N.,Bumbasirevic V.,Gregoric P.D.,Bajec D.J.D.,and Jeremic V.,2007,Risk factors for bleeding from stress ulcer in severely injured and critically ill persons,Acta Chir.Iugosl.,54(1):77-81
Silen W.,1988,Experimental models of gastric ulceration and injury,Am.J.Physiol.,255(1):395-402
Subbotina L.A.,2008,Stress effect on the blood aggregate state,Aviakosm.Ekolog.Med.,42(3):34-37
Syam A.F.,Simadibrata M.,Wanandi S.I.,Hernowo B.S.,Sadikin M.,and Rani A.A.,2011,Gastric ulcers induced by systemic hypoxia,Acta Med.Indones.,43(4):243-248
Tafti L.D.,Shariatpanahi S.M.,Damghani M.M.,and Javadi B.,2017,Tradition al persian topical medications for gastrointestinal diseases,Iran.J.Basic Med.Sci.,20(3):222-241
Wu T.Y.,Ding S.Q.,Liu J.L.,Jia J.H.,Chai Z.C.,and Dai R.C.,201 2,Who are more at risk for acute mountain sickness:a prospective study in Qinghai-Tibet railroad construction workers on Mt.Tanggula.,Chin.Med.J.,125(8):1393-1400
Wu T.Y.,Ding S.Q.,Liu J.L.,Jia J.H.,Dai R.C.,Zhu D.C.,Liang B.Z.,Qi D.T.,and Sun Y.F.,2007,High-altitude gastrointestinal bleeding:an observation in Qinghai-Tibetan railroad construction workers on Mountain Tanggula,World J.Gastroenterol.,13(5):774-780
Wu T.Y.,Ding S.Q.,Liu J.L.,Yu M.T.,Jia J.H.,Chai Z.C.,Dai R.C.,Zhang S.L.,Li B.Y.,Pan L.,Liang B.Z.,Zhao J.Z.,Qi de T.,Sun Y.F.,and Kayser B.,2007,Who should not go high:chronic disease and work at altitude during construction of the Qinghai-Tibet railroad,High Alt.Med.Biol.,8(2):88-107
Wu T.Y.,Ding S.Q.,Liu J.L.,Yu M.T.,Jia J.H.,Duan J.Q.,Chai Z.C.,Dai R.C.,Zhang S.L.,Liang B.Z.,Zhao J.Z.,Qi de T.,Sun Y.F.,and Kayser B.,2009,Reduced incidence and severity of acute mountain sickness in Qinghai-Tibet railroad construction workers after repeated 7-month exposures despite 5-month low altitude periods,High Alt.Med.Biol.,10(3):221-232
Yung H.W.,Cox M.,Tissot van Patot M.,and Burton G.J.,2012,Evidence of endoplasmic reticulum stress and protein synthesis inhibition in the placenta of non-native women at high altitude,FASEB J.,26(5):1970-1981
Zhao Y.,Luo C.,Lan X.M.,Liu Y.,and Sun Y.Z.,2016,Mechanism of Zhituo jiebai pills on gastric ulcer induced by acetic acid in mice,Journal of Jiangxi University of TCM,28:65-67