胎膜早破孕妇血清Caspase-1、IL-1β与IL-18的测定及意义
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摘要
目的:测定胎膜早破孕妇血清中Caspase-1、IL-1β与IL-18的水平,并分析其在胎膜早破发病中的意义。方法:用ELSIA法对35例胎膜早破孕妇和30例正常孕足月妇女血清中Caspase-1、IL-1β与IL-18进行测定。结果:与正常孕足月组相比,胎膜早破组血清中的Caspase-1、IL-1β与IL-18水平均明显高,差异有统计意义(P<0.05);胎膜早破妇女血清中Caspase-1与IL-1β呈正相关(P<0.01);也与IL-18呈正相关(P<0.01)。结论:Caspase-1、IL-1β与IL-18参与了胎膜早破的发病过程。
Objective:To determine levels of maternal serum Caspase-1,IL-1β and IL-18 in patients with premature rupture of membranes(PROM in order to analyze their significance in pathogenesis of PROM.Method:The levels of maternal serum Caspase-1,IL-1β and IL-18 of 35 patients with preterm premature rupture of membranes,30 term-pregnant women were detected by ELISA.Result:The levels of maternal serum Caspase-1,IL-1β and IL-18 of patients with preterm premature rupture of membranes were all higher than that of term-pregnant women(P<0.05).The levels of maternal serum Caspase-1 of patients with preterm premature rupture of membranes were positively correlated with IL-1β(P<0.01),so were Caspase-1 with IL-18(P<0.01).Conclusion:Caspase-1,IL-1β and IL-18 all take part in the pathogenesis of premature rupture of membranes.
Objective:To determine levels of maternal serum Caspase-1,IL-1β and IL-18 in patients with premature rupture of membranes(PROM in order to analyze their significance in pathogenesis of PROM.Method:The levels of maternal serum Caspase-1,IL-1β and IL-18 of 35 patients with preterm premature rupture of membranes,30 term-pregnant women were detected by ELISA.Result:The levels of maternal serum Caspase-1,IL-1β and IL-18 of patients with preterm premature rupture of membranes were all higher than that of term-pregnant women(P<0.05).The levels of maternal serum Caspase-1 of patients with preterm premature rupture of membranes were positively correlated with IL-1β(P<0.01),so were Caspase-1 with IL-18(P<0.01).Conclusion:Caspase-1,IL-1β and IL-18 all take part in the pathogenesis of premature rupture of membranes.
引文
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[19]Z uo G,Dong J X,Zhao F F,et al.Expression of matrix metalloproteinase-9 and its substrate level in patients with premature rupture of membranes[J].J Obstet Gynaecol,2017,37(4):441-445.
[20]Sukhikh G T,Kan N E,Tyutyunnik V L,et al.The role of extracellular inducer matrix metalloproteinases in premature rupture of membranes[J].J Matern Fetal Neonatal Med,2016,29(4):656-659.
[21]Cai Y L,Wang Z W.The expression and significance of IL-6,IFN-γ,SM22a,and MMP-2 in rat model of aortic dissection[J].Eur Rev Med Pharmacol Sci,2017,21(3):560-568.
[2]Zhu J,He M,Ma C,et al.Expression and Clinical Significant of NOD-Like Receptor Protein 3(NLRP3)and Caspase-1 in Fetal Membrane and Placental Tissues of Patients with Premature Rupture of Membrane[J].Med Sci Monit,2018,24:1560-1566.
[3]Fan S,Yuan J,Deng S,et al.Activation of interleukin-1?release by the classical swine fever virus is dependent on the NLRP3 inflammasome,which affects virus growth in monocytes[J].Front Cell Infect Microbiol,2018,8:225.
[4]Zhou W,Chen C,Chen Z,et al.NLRP3:A Novel Mediator in Cardiovascular Disease[J].J Immunol Res,2018,2018:5702103.
[5]Qu W,Wang Y,Wu Y,et al.Triggering receptors expressed on myeloid cells 2 promotes corneal resistance against pseudomonas aeruginosa by inhibiting caspase-1-dependent pyroptosis[J].Front Immunol,2018,9:1121.
[6]Mogami H,Hari Kishore A,Akgul Y,et al.Healing of Preterm Ruptured Fetal Membranes[J].Sci Rep,2017,7(1):13139.
[7]Man S M,Karki R,Kanneganti T D.Molecular mechanisms and function of pyroptosis,inflammatory caspases and inflammasomes in infectious disease[J].Immunol Rev,2017,277(1):61-75.
[8]Man S M,Karki R,Briard B,et al.Differential roles of caspase-1 and caspase-11 in infection and inflammation[J].Sci Rep,2017,7:45126.
[9]Eckhart L,Tschachler E.Control of cell death-associated danger signals during cornification prevents autoinflammation of the skin[J].Exp Dermatol,2018,27(8):884-891.
[10]Malik A,Kanneganti T D.Inflammasome activation and assembly at a glance[J].J Cell Sci,2017,130(23):3955-3963.
[11]Shao B Z,Xu Z Q,Han B Z,et al.NLRP3 inflammasome and its inhibitors:a review[J].Front Pharmacol,2015,5(6):262.
[12]Mulvihill E,Sborgi L,Mari SA,et al.Mechanism of membrane pore formation by human gasdermin-D[J].EMBO J,2018,37(14):e98321.
[13]DiPeso L,Ji D X,Vance R E,et al.Cell death and cell lysis are separable events during pyroptosis[J].Cell Death Discov,2017,13(3):17070.
[14]Feng S,Fox D,Man S M.Mechanisms of Gasdermin Family Members in Inflammasome Signaling and Cell Death[J].J Mol Biol,2018,430(18 Pt B):3068-3080.
[15]Heilig R,Dick M S,Sborgi L,et al.The Gasdermin-D pore acts as a conduit for IL-1?secretion in mice[J].Eur J Immunol,2018,48(4):584-592.
[16]Conos S A,Lawlor K E,Vaux D L,et al.Cell death is not essential for caspase-1-mediated interleulin-1?activation and secretion[J].Cell Death Differ,2016,23(11):1827-1838.
[17]Xu Y J,Zheng L,Hu Y W,et al.Pyroptosis and its relationship to atherosclerosis[J].Clin Chim Acta,2018,476:28-37.
[18]Chen M S,Lin C Y,Chiu Y H,et al.IL-1?-induced matrix metalloprotease-1 promotes mesenchymal stem cell migration via PAR1and G-protein-coupled signaling pathway[J].Stem Cells Int,2018,2018:3524759.
[19]Z uo G,Dong J X,Zhao F F,et al.Expression of matrix metalloproteinase-9 and its substrate level in patients with premature rupture of membranes[J].J Obstet Gynaecol,2017,37(4):441-445.
[20]Sukhikh G T,Kan N E,Tyutyunnik V L,et al.The role of extracellular inducer matrix metalloproteinases in premature rupture of membranes[J].J Matern Fetal Neonatal Med,2016,29(4):656-659.
[21]Cai Y L,Wang Z W.The expression and significance of IL-6,IFN-γ,SM22a,and MMP-2 in rat model of aortic dissection[J].Eur Rev Med Pharmacol Sci,2017,21(3):560-568.