艾灸神阙、足三里穴对痴呆大鼠瘦素、瘦素受体表达水平的影响
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摘要
目的 通过比较艾灸不同腧穴对痴呆(Alzheimer disease,AD)大鼠血清中瘦素(leptin),脂肪组织中瘦素、瘦素受体(leptin receptor)水平的表达,探讨艾灸神阙、足三里穴对AD大鼠脂质代谢的影响。方法 在55只大鼠中先随机抽取10只为空白对照组,剩余45只在造模成功后筛选出40只随机分为模型对照组、神阙组、足三里组、神阙-足三里组,每组10只。大鼠腹腔注射D-半乳糖、氢溴酸东莨菪碱制造痴呆模型,各治疗组艾炷灸相应腧穴,每穴3壮。用酶联免疫吸附法检测大鼠血清中瘦素的含量,用荧光定量RT-PCR法检测大鼠脂肪组织中瘦素mRNA的表达,用蛋白质免疫印迹法(Western blot)检测大鼠脂肪组织中瘦素受体蛋白的表达。结果 与模型组比较,各艾灸组大鼠血清中瘦素含量明显降低(P<0.01),瘦素受体含量明显增多(P<0.01),瘦素基因含量明显增多(P<0.05),各治疗组之间比较差异不明显(P>0.05)。结论 艾灸神阙、足三里穴均可提高血清中瘦素、脂肪组织中瘦素及瘦素受体水平。临床治疗AD可参照本实验研究结果选穴。
Objective Giving moxibustion to different experimental acupoints and made comparison to leptin, leptin in adipose tissue and leptin receptor in rats serum expression to discuss the metabolism effect by moxibustion Shen Que(RN 8) point and Zu San Li(ST 36) point. Methods Random drawing 10 rats as a normal group from 55 rats, then after successfully established experiment, 40 rats models were selected and divided as model group, Shen Que(RN 8) point group, Zu San Li(ST 36) point group and Shen Que-Zu San Li(RN 8-ST 36) group with 10 rats in each group. Rats were intraperitoneally infused with D-Gal, scopolamine hydrobromide to made the dementia model, and gave moxibustion treatment to three treatment group,each acupoint 3 cones, the leptin content were analyzed by ELISA method, using fluorescence quantitative RT-PCR to detect the genes expression of rats adipose tissue and detect the leptin receptor protein with Western Blot.Results The leptin concentration was decreased clearly(P<0.01) in every model groups and the content of leptin gene(P<0.05)and leptin receptor(P<0.01) was increased significantly. The curative effect of every treatment group were the same.Conclusion It could improve the leptin in serum, leptin in adipose tissue and leptin receptor level by giving moxibustion to Shen Que(RN 8) point, Zu San Li(ST 36) point and the clinical treatment of AD can refer to the results of this research.
Objective Giving moxibustion to different experimental acupoints and made comparison to leptin, leptin in adipose tissue and leptin receptor in rats serum expression to discuss the metabolism effect by moxibustion Shen Que(RN 8) point and Zu San Li(ST 36) point. Methods Random drawing 10 rats as a normal group from 55 rats, then after successfully established experiment, 40 rats models were selected and divided as model group, Shen Que(RN 8) point group, Zu San Li(ST 36) point group and Shen Que-Zu San Li(RN 8-ST 36) group with 10 rats in each group. Rats were intraperitoneally infused with D-Gal, scopolamine hydrobromide to made the dementia model, and gave moxibustion treatment to three treatment group,each acupoint 3 cones, the leptin content were analyzed by ELISA method, using fluorescence quantitative RT-PCR to detect the genes expression of rats adipose tissue and detect the leptin receptor protein with Western Blot.Results The leptin concentration was decreased clearly(P<0.01) in every model groups and the content of leptin gene(P<0.05)and leptin receptor(P<0.01) was increased significantly. The curative effect of every treatment group were the same.Conclusion It could improve the leptin in serum, leptin in adipose tissue and leptin receptor level by giving moxibustion to Shen Que(RN 8) point, Zu San Li(ST 36) point and the clinical treatment of AD can refer to the results of this research.
引文
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[13] 关大顺.现代代谢病与营养性疾病[M].天津:天津科技翻译出版公司,2008:252.
[14] 张慧,何屹峰,李爱萍.瘦素在脑老化认知发展不同阶段中的作用[J].生理科学进展,2015,46(5):321.
[15] Perez-Gonzalez R,Antequera D,Warqaw T,et al.Leptin induces proliferation of neuronal progenitors and neuroprotection in a mouse model of Alzheimer’s disease[J].J Alzheimer’s Dis,2011,24(Suppl):217.
[2] 肖移生,曾元凤,欧阳厚淦,等.黄精地龙方对老年痴呆大鼠行为认知及脑胆碱能系统的影响[J].中药药理与临床,2013,29(4):146.
[3] 李忠仁.实验针灸学[M].北京:中国中医药出版社,2003:327.
[4] 王海军,冀来喜.关于大鼠神阙穴的定位[J].针刺研究,2007,32(5):312.
[5] 石学敏.针灸学[M].北京:中国中医药出版社,2014:230.
[6] 谭杏,杨茜芸,林亚平.艾灸足三里穴对衰老大鼠学习记忆及抗疲劳能力的影响[J].中国老年学杂志,2015,12(35):6976.
[7] 赵彩娇,李娴,覃永贞,等.艾灸足三里对衰老模型豚鼠血清SOD及肝肾组织基因表达的影响[J].时珍国医国药,2016,27(10):2543.
[8] 王凤玲,李蕙,魏正岫,等.灸神阙穴对中老年人免疫功能及其全身状态的影响[J].中国针灸,1996,16(7):39.
[9] 唐英,马彩云,尚清,等.艾灸对脑瘫患儿免疫功能的影响[J].中国针灸,2016,36(1):12.
[10] 杨裕华,王际莘,鲁燕.糖脂代谢对老年痴呆影响的研究进展[J].广东医学,2012,33(18):2849.
[11] 王刚,任汝静.阿尔茨海默病及其他类型痴呆的临床实践指南[M].上海:上海交通大学出版社,2015:1.
[12] 韩亚,宋荣蓉.脂联素与血管性痴呆危险因素关系的研究进展[J].医学综述,2016,22(12):2313.
[13] 关大顺.现代代谢病与营养性疾病[M].天津:天津科技翻译出版公司,2008:252.
[14] 张慧,何屹峰,李爱萍.瘦素在脑老化认知发展不同阶段中的作用[J].生理科学进展,2015,46(5):321.
[15] Perez-Gonzalez R,Antequera D,Warqaw T,et al.Leptin induces proliferation of neuronal progenitors and neuroprotection in a mouse model of Alzheimer’s disease[J].J Alzheimer’s Dis,2011,24(Suppl):217.