改性粘土与抑藻菌耦合法抑制锥状斯氏藻研究
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摘要
为了开发高效和环境友好型的抑藻方法,作者研究了2种改性粘土(十六烷基三甲基溴化铵和三丁基十六烷基溴化磷)与抑藻菌联合使用消杀锥状斯氏藻(Scrippsiellatrochoidea)的效果.结果表明,单独使用改性粘土时,十六烷基三甲基溴化铵的效果优于三丁基十六烷基溴化磷,但只能在初期(48h内)起到较强的抑制作用,对藻细胞没有彻底去除.将改性粘土与抑藻细菌(蜡样芽孢杆菌Bacillus cereus G4,纺锤形赖氨酸芽孢杆菌Lysinibacillus fusiformis J8)进行耦合后,抑藻效果明显而持续,有效抑藻时间可持续168 h,且未见叶绿素含量明显的反弹.因此,十六烷基三甲基溴化铵改性粘土与抑藻菌联合使用有更好的成效,可作为一种极具潜力的环境友好型方法用于有害藻类的治理.
In order to develop a highly efficient and environment-friendly algal inhibition method, the effect of two kinds of modified clay(sixteen alkyl three methyl ammonium bromide and three butylsixteen alkyl bromide) combined with algae inhibition bacteria on killing Scrippsiella trochoidea was studied. The result showed that the inhibitory effect of sixteen alkyl three methyl ammonium bromide was better than that of three butyl sixteen alkyl bromide when the modified clay was used alone, but only in the initial stage(48 hours). However, the algae cells were not completely removed. When the modified clay was coupled with algae inhibition bacteria(Bacillus cereus G4 and Lysinibacillusfusiformis J8), the effect of algae inhibition was obvious and persistent, and the effective algal inhibition time could last up to 168 hours without obvious rebound of chlorophyll concentration. Therefore, our study shows that the combined use of methyltrimethyl-ammonium bromide modified clay and algal inhibition bacteria can be used as a potential environment-friendly method to control harmful alage.
In order to develop a highly efficient and environment-friendly algal inhibition method, the effect of two kinds of modified clay(sixteen alkyl three methyl ammonium bromide and three butylsixteen alkyl bromide) combined with algae inhibition bacteria on killing Scrippsiella trochoidea was studied. The result showed that the inhibitory effect of sixteen alkyl three methyl ammonium bromide was better than that of three butyl sixteen alkyl bromide when the modified clay was used alone, but only in the initial stage(48 hours). However, the algae cells were not completely removed. When the modified clay was coupled with algae inhibition bacteria(Bacillus cereus G4 and Lysinibacillusfusiformis J8), the effect of algae inhibition was obvious and persistent, and the effective algal inhibition time could last up to 168 hours without obvious rebound of chlorophyll concentration. Therefore, our study shows that the combined use of methyltrimethyl-ammonium bromide modified clay and algal inhibition bacteria can be used as a potential environment-friendly method to control harmful alage.
引文
[1]闰新亚,杨维东,谭绍早.不同有机改性黏土对海洋卡盾藻去除作用的研究[J].安全与环境学报,2010,10(6):45-48.
[2]周名江,朱明远,张经.中国赤潮的发生趋势和研究进展[J].生命科学,2001,13(2):54-59.
[3]杨维东,刘玉荣,刘洁生,等.桉木粉对塔玛亚历山大藻的抑制作用及其化学基础研究[J].环境科学,2008,29(8):2296-2301.
[4]杨维东,欧阳妤婧,刘洁生.玉米秸秆对塔玛亚历山大藻生长的影响及化学基础研究[J].环境科学,2008,29(9):2470-2474.
[5]俞志明,邹景忠,马锡年.一种提高粘土矿物去除赤潮生物能力的新方法[J].海洋与湖沼,1994,25(2):226-232.
[6]王新,周艳艳,郑天凌.海洋细菌生态学的若干前沿课题及其研究新进展[J].微生物学报,2010,50(3):291-297.
[7]张勇,席宇,吴刚.溶藻细菌杀藻物质的研究进展[J].微生物学通报,2004,31(1):127-132.
[8]史荣君,黄洪辉,齐占会,等.一株溶藻细菌对海洋原甲藻的溶藻效应[J].生态学报,2012,2(16):4993-5001.
[9]俞志明,宋秀贤,张波.粘土表面改性及对赤潮生物絮凝作用研究[J].科学通报,1999,44(3):308-311.
[10]俞志明,马锡年,谢阳.黏土矿物对海水中主要营养盐的吸附研究[J].海洋与湖沼,1995,26(2):208-214.
[11]Miao C,Tang Y,Zhang H,et al.Harmful algae blooms removal from fresh water with modified vermiculite[J].Environmental Technology,2014,35(1-4):340-346.
[12]王闪,姚丹义,陈微.改性黏土去除水华束丝藻的研究[J].安全与环境学报,2016,16(10):242-247.
[13]高咏卉,俞志明,宋秀贤,等.有机改性黏土对海水中营养盐及主要水质因子的影响[J].海洋科学,2007,31(8):30-37.
[14]Certner R,Cho H,Gallo N,et al.Using sediment flocculation to reduce the impacts of Chesapeake Bay Microcystis aeruginosa harmful algal blooms[J/OL].http://agris.fao.org/openagris/search.do?recordID=AV2012056170.AGIRS,2011.
[15]俞志明,Subba Rao.黏土矿物对尖刺拟菱形藻多列型生长和藻毒素产生的影响[J].海洋与湖沼,1998,29(1):47-52.
[16]Lang P,Meis S,ProcházkováL,et al.Phytoplankton community responses in a shallow lake following lanthanum-bentonite application[J].Water Research,2016,97:55-68.
[17]齐雨藻.中国沿海赤潮[M].北京:科学出版社,2003:104-249.
[18]Lu G,Song X,Yu Z,et al.Environmental effects of modified clay flocculation on Alexandrium tamarense and paralytic shellfish poisoning toxins(PSTs)[J].Chemosphere,2015,127:188-94.
[19]Wang Z,Zhang H,Pan G.Ecotoxicological assessment of flocculant modified soil for lake restoration using an integrated biotic toxicity index[J].Water Research,2016,97:133-41.
[20]Liu Y,Cao X,Yu Z,et al.Controlling harmful algae blooms using aluminum-modified clay[J].Marine Pollution Bulletin,2016,103(1-2):211-219.
[21]Li J,Song X,Zhang Y,et al.An investigation of the space distribution of Ulva microscopic propagules and shipbased experiment of mitigation using modified clay[J].Marine Pollution Bulletin,2017,117(1-2):247-254.
[2]周名江,朱明远,张经.中国赤潮的发生趋势和研究进展[J].生命科学,2001,13(2):54-59.
[3]杨维东,刘玉荣,刘洁生,等.桉木粉对塔玛亚历山大藻的抑制作用及其化学基础研究[J].环境科学,2008,29(8):2296-2301.
[4]杨维东,欧阳妤婧,刘洁生.玉米秸秆对塔玛亚历山大藻生长的影响及化学基础研究[J].环境科学,2008,29(9):2470-2474.
[5]俞志明,邹景忠,马锡年.一种提高粘土矿物去除赤潮生物能力的新方法[J].海洋与湖沼,1994,25(2):226-232.
[6]王新,周艳艳,郑天凌.海洋细菌生态学的若干前沿课题及其研究新进展[J].微生物学报,2010,50(3):291-297.
[7]张勇,席宇,吴刚.溶藻细菌杀藻物质的研究进展[J].微生物学通报,2004,31(1):127-132.
[8]史荣君,黄洪辉,齐占会,等.一株溶藻细菌对海洋原甲藻的溶藻效应[J].生态学报,2012,2(16):4993-5001.
[9]俞志明,宋秀贤,张波.粘土表面改性及对赤潮生物絮凝作用研究[J].科学通报,1999,44(3):308-311.
[10]俞志明,马锡年,谢阳.黏土矿物对海水中主要营养盐的吸附研究[J].海洋与湖沼,1995,26(2):208-214.
[11]Miao C,Tang Y,Zhang H,et al.Harmful algae blooms removal from fresh water with modified vermiculite[J].Environmental Technology,2014,35(1-4):340-346.
[12]王闪,姚丹义,陈微.改性黏土去除水华束丝藻的研究[J].安全与环境学报,2016,16(10):242-247.
[13]高咏卉,俞志明,宋秀贤,等.有机改性黏土对海水中营养盐及主要水质因子的影响[J].海洋科学,2007,31(8):30-37.
[14]Certner R,Cho H,Gallo N,et al.Using sediment flocculation to reduce the impacts of Chesapeake Bay Microcystis aeruginosa harmful algal blooms[J/OL].http://agris.fao.org/openagris/search.do?recordID=AV2012056170.AGIRS,2011.
[15]俞志明,Subba Rao.黏土矿物对尖刺拟菱形藻多列型生长和藻毒素产生的影响[J].海洋与湖沼,1998,29(1):47-52.
[16]Lang P,Meis S,ProcházkováL,et al.Phytoplankton community responses in a shallow lake following lanthanum-bentonite application[J].Water Research,2016,97:55-68.
[17]齐雨藻.中国沿海赤潮[M].北京:科学出版社,2003:104-249.
[18]Lu G,Song X,Yu Z,et al.Environmental effects of modified clay flocculation on Alexandrium tamarense and paralytic shellfish poisoning toxins(PSTs)[J].Chemosphere,2015,127:188-94.
[19]Wang Z,Zhang H,Pan G.Ecotoxicological assessment of flocculant modified soil for lake restoration using an integrated biotic toxicity index[J].Water Research,2016,97:133-41.
[20]Liu Y,Cao X,Yu Z,et al.Controlling harmful algae blooms using aluminum-modified clay[J].Marine Pollution Bulletin,2016,103(1-2):211-219.
[21]Li J,Song X,Zhang Y,et al.An investigation of the space distribution of Ulva microscopic propagules and shipbased experiment of mitigation using modified clay[J].Marine Pollution Bulletin,2017,117(1-2):247-254.