脱氧雪腐镰刀菌烯醇时间分辨荧光免疫检测体系适用性评价
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摘要
目的研究已建立的脱氧雪腐镰刀菌烯醇时间分辨荧先兊疫定量检测体系(包括脱氧雪腐镰刀菌烯醇时间分辨荧先兊疫层析检测卡和荧先定量检测仪)的适用性。方法检测20个阴性样本中脱氧雪腐镰刀菌烯醇含量,确定该检测体系的检出限和定量限;对6组含不同浓度脱氧雪腐镰刀菌烯醇的小麦阳性样本和6组含不同浓度脱氧雪腐镰刀菌烯醇的玉米阳性样本进行检测,确定方法的准确性和台间差;重复6次检测含中等浓度脱氧雪腐镰刀菌烯醇的阳性样本和连续12 h检测国家检测限浓度的阳性样本,确定系统的重复性和稳定性。结果该体系的检出限为154μg/kg,定量限为414μg/kg,幵且检测结果与液质联用方法定值结果无显著性差异,仪器台间差无显著差异;测值的精密度未超过国家标准规定要求。结论本研究验证的时间分辩荧先定量检测体系能准确检测玉米和小麦样本中脱氧雪腐镰刀菌烯醇含量。
Objective To investigate the applicability of the established quantitative detection system for the time-resolved fluoro-immunoassay(TRFIA) of deoxynivalenol,including TRFIA quantitative test strips and quantitative detector.Methods The limit of detection and limit of quantitative of the detection system were determined by detecting the content of deoxynivalenol in 20 negative samples.Totally 6 groups of wheat positive samples with different concentrations of deoxynivalenol and 6 groups of maize positive samples with different concentrations of deoxynivalenol were detected,in order to determine the accuracy and inter-station difference of the method.Through 6 times of detection of positive samples with middle concentration of deoxynivalenol,and detection of the positive samples with the national detection limit concentration for 12 consecutive hours,the repeatability of the system was determined.Results The limit of detection for this system was 154 μg/kg and limit of quantitation was 414 μg/kg,and there were no significant differences between detection value and fixed value and between instruments.The precision of the measured value did not exceed the requirements of national standards.Conclusion This verified deoxynivalenol time-resolved fluoro-immunoassay detection system can accurately detect the content of deoxygenated fusarium oxygenate in corn and wheat samples.
Objective To investigate the applicability of the established quantitative detection system for the time-resolved fluoro-immunoassay(TRFIA) of deoxynivalenol,including TRFIA quantitative test strips and quantitative detector.Methods The limit of detection and limit of quantitative of the detection system were determined by detecting the content of deoxynivalenol in 20 negative samples.Totally 6 groups of wheat positive samples with different concentrations of deoxynivalenol and 6 groups of maize positive samples with different concentrations of deoxynivalenol were detected,in order to determine the accuracy and inter-station difference of the method.Through 6 times of detection of positive samples with middle concentration of deoxynivalenol,and detection of the positive samples with the national detection limit concentration for 12 consecutive hours,the repeatability of the system was determined.Results The limit of detection for this system was 154 μg/kg and limit of quantitation was 414 μg/kg,and there were no significant differences between detection value and fixed value and between instruments.The precision of the measured value did not exceed the requirements of national standards.Conclusion This verified deoxynivalenol time-resolved fluoro-immunoassay detection system can accurately detect the content of deoxygenated fusarium oxygenate in corn and wheat samples.
引文
[1]刘娜,武爱波.真菌毒素快速检测技术研究进展[J].食品安全质量检测学报,2014,5(7):1965-1970.Liu N,Wu AB.Methodological advances for rapid detection of mycotoxins[J].Food Saf Qual,2014,5(7):1965-1970.
[2]张晓莉,孙伟,张红印,等.脱氧雪腐镰刀菌烯醇的毒性及生物脱毒研究进展[J].食品科学,2016,37(17):245-251.Zhang XL,Sun W,Zhang HY,et al.Progress in toxicity and biological detoxification of deoxynivalenol[J].Food Sci,2016,37(17):245-251.
[3]谢丹,邓春丼,赵云峰,等.北京地区部分市售食用植物油中玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇的污染状况分析[J].食品安全质量检测学报,2016,7(5):2105-2113.Xie D,Deng CL,Zhao YF,et al.Investigation of the contamination status of zearalenone and deoxynivalenol in edible oil[J].J Food Saf Qual,2016,7(5):2105-2113.
[4]GB 2761-2017食品安全国家标准食品中真菌毒素限量[S].GB 2761-2017 National food safety standard-Fungal toxins in food[S].
[5]Pestka JJ,Zhou HR,Moon Y,et al.Cellular and molecular mechanisms for immunemodulation by deoxyni-valenol and other trichothecenes:unraveling a paradox[J].Toxicol Lctt,2004,153(1):61-73.
[6]No 1881/2006 Setting maximum levels for certain contaminants in foodstuffs[S].
[7]Anukul N,Vangnai K,Mahakarnchanakul K.Significance of regulation limits in mycotoxin contamination in Asia and risk management programs at the national level[J].J Food Drug Anal,2013,21(3):227-241.
[8]Toxin derid from fusarium grainearum,F.Culmorum and F.Crookwellense:zearalenone,deoxynivalenol,nivalenol and fusarenone X[EB/OL].http://monographs.iarc.fr/ENG/Monographs/vol56/mono56-15.
[9]FDA Regulatory Guidance for Mycotoxins:A Guide for Grain Elevators,Feed Manufacturers,Grain Processors and Exporte rs[EB/OL].https://www.ngfa.org/wp-content/uploads/NGFACompliance GuideFDARegulatory Guidancefor Mycotoxins8-2011.
[10]李萌,冯才伟,贾芳芳,等.一种快速检测呕吐毒素的胶体金试纸条[J].中国酿造,2013,32(11):91-93.Li M,Feng CW,Jia FF,et al.A rapid test strips for vomitoxin[J].China Brew,2013,32(11):91-93.
[11]郭健,王郑平,曹阳,等.高效液相色谱法测定粮食中的脱氧雪腐镰刀菌烯醇[J].粮食与食品工业,2014,21(6):87-89.Guo J,Wang ZP,Cao Y,et al.Study on rapid determination of deoxynivalenol in grain by UPLC[J].Cere Food Ind,2014,21(6):87-89.
[12]崔晓娜,李舫,王洪涛,等.高效液相色谱串联质谱测定粮谷及饲料中玉米赤霉烯酮及其代谢物和伏马毒素B1、B2[J].中国畜牧杂志,2015,51(4):62-66.Cui XN,Li H,Wang HT,et al.Detemination of zearaleimne and its metabolites and fumonisins Bl and B2 in the grain and feed with HPLC-MS/MS[J].Chin J Anim Sci,2015,51(4):62-66.
[13]王俊双,许定花,孙秀兰.食品中脱氧雪腐镰刀菌烯醇三种检测方法的比较[J].食品工业科技,2008,29(2):287-290.Wang JS,Xu DH,Sun XL.Comparasion of the detection methods for deoxynivalenol in corn[J].Sci Technol Food Ind,2008,29(2):287-290.
[14]周彬,高雷,金坚,等.纳米均相时间分辨荧先兊疫法检测脱氧雪腐镰刀菌烯醇方法的建立[J].食品工业科技,2010,31(8):338-342.Zhou B,Gao L,Jin J,et al.Quantitative determination of deoxynivalenol using light initiated chemiluminescence assay[J].Sci Technol Food Ind,2010,31(8):338-342.
[15]张兆威,李培武,张奇,等.农产品中黄曲霉毒素的时间分辨荧先兊疫层析快速检测技术研究[J].中国农业科学,2014,47(18):3668-3674.Zhang ZW,Li PW,Zhang Q,et al.Study on time-resolved fluorescence immunochromatography for afaltoxin determination in agricultural product[J].Sci Agric Sin,2014,47(18):3668-3674.
[16]LS/T 6402-2017粮油检验设备和方法标准适用性验证及结果评价一般原则[S].LS/T 6402-2017 Grain and Oil inspection-General principles for verification of applicability of equipment and method standards and evaluation of results[S].
[17]GB/T 4889-2008数据的统计处理和解释正态分布均值和方差的估计与检验[S].GB/T 4889-2008 Statistical processing and interpretation of data-The Estimation and test of the mean and variance of normal distribution[S].
[2]张晓莉,孙伟,张红印,等.脱氧雪腐镰刀菌烯醇的毒性及生物脱毒研究进展[J].食品科学,2016,37(17):245-251.Zhang XL,Sun W,Zhang HY,et al.Progress in toxicity and biological detoxification of deoxynivalenol[J].Food Sci,2016,37(17):245-251.
[3]谢丹,邓春丼,赵云峰,等.北京地区部分市售食用植物油中玉米赤霉烯酮和脱氧雪腐镰刀菌烯醇的污染状况分析[J].食品安全质量检测学报,2016,7(5):2105-2113.Xie D,Deng CL,Zhao YF,et al.Investigation of the contamination status of zearalenone and deoxynivalenol in edible oil[J].J Food Saf Qual,2016,7(5):2105-2113.
[4]GB 2761-2017食品安全国家标准食品中真菌毒素限量[S].GB 2761-2017 National food safety standard-Fungal toxins in food[S].
[5]Pestka JJ,Zhou HR,Moon Y,et al.Cellular and molecular mechanisms for immunemodulation by deoxyni-valenol and other trichothecenes:unraveling a paradox[J].Toxicol Lctt,2004,153(1):61-73.
[6]No 1881/2006 Setting maximum levels for certain contaminants in foodstuffs[S].
[7]Anukul N,Vangnai K,Mahakarnchanakul K.Significance of regulation limits in mycotoxin contamination in Asia and risk management programs at the national level[J].J Food Drug Anal,2013,21(3):227-241.
[8]Toxin derid from fusarium grainearum,F.Culmorum and F.Crookwellense:zearalenone,deoxynivalenol,nivalenol and fusarenone X[EB/OL].http://monographs.iarc.fr/ENG/Monographs/vol56/mono56-15.
[9]FDA Regulatory Guidance for Mycotoxins:A Guide for Grain Elevators,Feed Manufacturers,Grain Processors and Exporte rs[EB/OL].https://www.ngfa.org/wp-content/uploads/NGFACompliance GuideFDARegulatory Guidancefor Mycotoxins8-2011.
[10]李萌,冯才伟,贾芳芳,等.一种快速检测呕吐毒素的胶体金试纸条[J].中国酿造,2013,32(11):91-93.Li M,Feng CW,Jia FF,et al.A rapid test strips for vomitoxin[J].China Brew,2013,32(11):91-93.
[11]郭健,王郑平,曹阳,等.高效液相色谱法测定粮食中的脱氧雪腐镰刀菌烯醇[J].粮食与食品工业,2014,21(6):87-89.Guo J,Wang ZP,Cao Y,et al.Study on rapid determination of deoxynivalenol in grain by UPLC[J].Cere Food Ind,2014,21(6):87-89.
[12]崔晓娜,李舫,王洪涛,等.高效液相色谱串联质谱测定粮谷及饲料中玉米赤霉烯酮及其代谢物和伏马毒素B1、B2[J].中国畜牧杂志,2015,51(4):62-66.Cui XN,Li H,Wang HT,et al.Detemination of zearaleimne and its metabolites and fumonisins Bl and B2 in the grain and feed with HPLC-MS/MS[J].Chin J Anim Sci,2015,51(4):62-66.
[13]王俊双,许定花,孙秀兰.食品中脱氧雪腐镰刀菌烯醇三种检测方法的比较[J].食品工业科技,2008,29(2):287-290.Wang JS,Xu DH,Sun XL.Comparasion of the detection methods for deoxynivalenol in corn[J].Sci Technol Food Ind,2008,29(2):287-290.
[14]周彬,高雷,金坚,等.纳米均相时间分辨荧先兊疫法检测脱氧雪腐镰刀菌烯醇方法的建立[J].食品工业科技,2010,31(8):338-342.Zhou B,Gao L,Jin J,et al.Quantitative determination of deoxynivalenol using light initiated chemiluminescence assay[J].Sci Technol Food Ind,2010,31(8):338-342.
[15]张兆威,李培武,张奇,等.农产品中黄曲霉毒素的时间分辨荧先兊疫层析快速检测技术研究[J].中国农业科学,2014,47(18):3668-3674.Zhang ZW,Li PW,Zhang Q,et al.Study on time-resolved fluorescence immunochromatography for afaltoxin determination in agricultural product[J].Sci Agric Sin,2014,47(18):3668-3674.
[16]LS/T 6402-2017粮油检验设备和方法标准适用性验证及结果评价一般原则[S].LS/T 6402-2017 Grain and Oil inspection-General principles for verification of applicability of equipment and method standards and evaluation of results[S].
[17]GB/T 4889-2008数据的统计处理和解释正态分布均值和方差的估计与检验[S].GB/T 4889-2008 Statistical processing and interpretation of data-The Estimation and test of the mean and variance of normal distribution[S].