桔梗、白花蛇舌草不同组分对人肺癌细胞A549的抑制作用
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摘要
为筛选桔梗和白花蛇舌草中的抗肿瘤活性成分,制备了桔梗总黄酮、总皂苷提取物,白花蛇舌草总黄酮、总蒽醌提取物,采用亚硝酸钠-硝酸铝比色法检测总黄酮含量,香草醛法检测总皂苷含量,紫外吸收法检测总蒽醌含量,采用四甲基偶氮唑蓝比色法(MTT)检测4种提取物对人肺癌细胞A549的抑制作用。结果表明:桔梗和白花蛇舌草总黄酮含量分别为5.51%和1.19%,桔梗总皂苷含量为1.71%,白花蛇舌草总蒽醌含量为7.81%。桔梗总皂苷浓度为0.04 mg/mL时,对人肺癌细胞A549抑制率达到87.59%,桔梗总黄酮浓度为0.5 mg/mL时,抑制率达到89.16%。白花蛇舌草总蒽醌浓度为1.2 mg/mL时,抑制率达到34.76%;白花蛇舌草总黄酮浓度为0.3 mg/mL时,抑制率达到24.33%。试验结果显示,桔梗总黄酮和总皂苷对肺癌细胞A549的抑制作用较强,可为抗肿瘤药物的研究提供参考。
To screen the antitumor active component of Platycodon grandiflorum(Jacq.) A.DC. and Hedyotis diffusa Willd, total flavonoids and total saponins of Platycodon grandiflorum, total flavonoids and totalanthraquinones of Hedyotis diffusa Willd were prepared. The content of total flavonoids was determined bysodium nitrite-aluminum nitrate colorimetric method, the content of total saponins was determined by vanillinassay, and the content of total anthraquinones was determined by ultraviolet spectromter method. Theinhibitory effects of 4 extracts on human lung cancer cells A549 were determined by methyl thiazolyltetrazolium(MTT) assay. The results showed that, the contents of total flavonoids from Platycodon grandiflorum and Hedyotis diffusa Willd were 5.51% and 1.19%, the content of total saponins from Platycodon grandiflorum was 1.71%, the content of total anthraquinones from Hedyotis diffusa Willd was 7.81%. The inhibition rate of0.04 mg/mL total saponins from Platycodon grandiflorum on human lung cancer cells A549 was 87.59%, theinhibition rate of 0.5 mg/mL total flavonoids from Platycodon grandiflorum was 89.16%, the inhibition rate of1.2 mg/mL total anthraquinones from Hedyotis diffusa Willd was 34.76%, the inhibition rate of 0.3 mg/mL totalflavonoids from Hedyotis diffusa Willd was 24.33%. Taken together, total flavonoids and saponins from Platycodon grandiflorum have strong antitumor activity, which provides some powerful reference for thedevelopment of related antitumor products.
To screen the antitumor active component of Platycodon grandiflorum(Jacq.) A.DC. and Hedyotis diffusa Willd, total flavonoids and total saponins of Platycodon grandiflorum, total flavonoids and totalanthraquinones of Hedyotis diffusa Willd were prepared. The content of total flavonoids was determined bysodium nitrite-aluminum nitrate colorimetric method, the content of total saponins was determined by vanillinassay, and the content of total anthraquinones was determined by ultraviolet spectromter method. Theinhibitory effects of 4 extracts on human lung cancer cells A549 were determined by methyl thiazolyltetrazolium(MTT) assay. The results showed that, the contents of total flavonoids from Platycodon grandiflorum and Hedyotis diffusa Willd were 5.51% and 1.19%, the content of total saponins from Platycodon grandiflorum was 1.71%, the content of total anthraquinones from Hedyotis diffusa Willd was 7.81%. The inhibition rate of0.04 mg/mL total saponins from Platycodon grandiflorum on human lung cancer cells A549 was 87.59%, theinhibition rate of 0.5 mg/mL total flavonoids from Platycodon grandiflorum was 89.16%, the inhibition rate of1.2 mg/mL total anthraquinones from Hedyotis diffusa Willd was 34.76%, the inhibition rate of 0.3 mg/mL totalflavonoids from Hedyotis diffusa Willd was 24.33%. Taken together, total flavonoids and saponins from Platycodon grandiflorum have strong antitumor activity, which provides some powerful reference for thedevelopment of related antitumor products.
引文
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[2]卢红阳,蔡菊芬,毛伟敏.非小细胞肺癌辅助靶向治疗研究进展[J].肿瘤学杂志,2014,20(1):64-67.
[3]陈影,杜向慧.非小细胞肺癌免疫治疗临床新进展[J].医学研究杂志,2015,44(5):12-15.
[4]张莹,贾英杰,李小江,等.注射用黄芪多糖联合CIK细胞治疗中晚期气虚型非小细胞肺癌的临床观察[J].中草药,2018(7):1647-1651.
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[9] Zhang Z, Zhao M, Zheng W, et al. Platycodin D, a triterpenoid saponin from Platycodon grandiflorum, suppresses the growth and invasion of human oral squamous cell carcinoma cells via the NF-κB pathway[J].Journal of Biochemical&Molecular Toxicology,2017,31(9):e21934.
[10] Seo Y S, Kang O H, Kong R, et al. Polygalacin D induces apoptosis and cell cycle arrest via the PI3K/Akt pathway in non-small cell lung cancer[J]. Oncology Reports, 2018 39(4):1702-1710.
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[12]黄慧,田素英.白花蛇舌草总黄酮的超声提取工艺研究[J].中国当代医药,2011,18(23):144-146.
[13]崔健,施松善,王顺春,等.白花蛇舌草的化学成分及药理作用研究进展[J].上海中医药杂志,2005,39(7):57-59.
[14]施峰,贾晓斌,贾东升,等.白花蛇舌草预防肺癌物质基础研究[J].中华中医药杂志,2010(3):403-408.
[15]车杭盈.白花蛇舌草注射液诱导非小细胞肺癌A549细胞凋亡的机制研究[J].中西医结合研究,2014(1):20-22.
[16]杨楠,贾晓斌,张振海,等.黄酮类化合物抗肿瘤活性及机制研究进展[J].中国中药杂志,2015,40(3):373-381.
[17]孙晓润,陈苹苹,林悦,等.天然黄酮类化合物抗肿瘤作用靶点研究进展[J].中国实验方剂学杂志,2017(6):218-228.
[18]张彦兵,王天昶,朱娇,等.白花蛇舌草总黄酮对肝癌MHCC97-H细胞增殖的抑制作用[J].现代肿瘤医学,2015,23(19):2716-2719.
[19]谭玲玲,王忠,胡正海.桔梗中总黄酮的提取工艺优化[J].西北药学杂志,2014,29(06):568-570.
[20]孙乐,李春梅,郭迎迎,等.人参花中总皂苷的提取工艺优选[J].中国实验方剂学杂志,2015,21(01):21-23.
[21]卫亚丽,钟箫,汤洪敏.茜草中总蒽醌的提取工艺优化[J].江苏农业科学,2014,42(08):268-270.
[22]赵若琳,周坤福,张旭,等.桔梗皂苷-D抑制非小细胞肺癌H460和A549细胞黏附、侵袭和迁移的作用机制研究[J].中国药理学通报,2015(2):241-247.
[23]赵若琳,张旭,陈美娟,等.桔梗皂苷-D抗肿瘤机制研究进展[J].中国中医药信息杂志,2015,22(2):126-129.
[24]万小旭,张行,王佳贺.白花蛇舌草的有效成分2-羟基-3-甲基蒽醌通过Fas/FasL信号通路诱导卵巢癌细胞凋亡[J].实用药物与临床,2015,18(12):1405-1409.
[25]梁欣娜,张兴燊,滕红丽.中药及其有效成分抗肿瘤作用研究[J].时珍国医国药,2013,24(1):119-122.
[2]卢红阳,蔡菊芬,毛伟敏.非小细胞肺癌辅助靶向治疗研究进展[J].肿瘤学杂志,2014,20(1):64-67.
[3]陈影,杜向慧.非小细胞肺癌免疫治疗临床新进展[J].医学研究杂志,2015,44(5):12-15.
[4]张莹,贾英杰,李小江,等.注射用黄芪多糖联合CIK细胞治疗中晚期气虚型非小细胞肺癌的临床观察[J].中草药,2018(7):1647-1651.
[5]钟天飞,焦一凤,潘洁莉,等.中药作用人肺癌A549细胞的实验研究进展[J].中华中医药杂志,2015(10):3601-3606.
[6]成娅婷,田成旺,任涛,等.中药治疗非小细胞肺癌的临床应用及作用机制研究进展[J].药物评价研究,2016,39(2):289-295.
[7]郁梅,方彭华,于桂芳,等.桔梗的化学成分和抗肿瘤活性研究进展[J].国际药学研究杂志,2011,38(4):280-283.
[8]孙晓春,张伟强,唐平生,等.桔梗提取物的主要成分和抗氧化性的测定[J].中国农学通报,2017,33(31):139-144.
[9] Zhang Z, Zhao M, Zheng W, et al. Platycodin D, a triterpenoid saponin from Platycodon grandiflorum, suppresses the growth and invasion of human oral squamous cell carcinoma cells via the NF-κB pathway[J].Journal of Biochemical&Molecular Toxicology,2017,31(9):e21934.
[10] Seo Y S, Kang O H, Kong R, et al. Polygalacin D induces apoptosis and cell cycle arrest via the PI3K/Akt pathway in non-small cell lung cancer[J]. Oncology Reports, 2018 39(4):1702-1710.
[11]易亚乔,伍参荣,葛金文,等.桔梗总皂苷对人肺腺癌A549细胞抑制作用研究[J].中华中医药杂志.2012(28):7:2135-2137.
[12]黄慧,田素英.白花蛇舌草总黄酮的超声提取工艺研究[J].中国当代医药,2011,18(23):144-146.
[13]崔健,施松善,王顺春,等.白花蛇舌草的化学成分及药理作用研究进展[J].上海中医药杂志,2005,39(7):57-59.
[14]施峰,贾晓斌,贾东升,等.白花蛇舌草预防肺癌物质基础研究[J].中华中医药杂志,2010(3):403-408.
[15]车杭盈.白花蛇舌草注射液诱导非小细胞肺癌A549细胞凋亡的机制研究[J].中西医结合研究,2014(1):20-22.
[16]杨楠,贾晓斌,张振海,等.黄酮类化合物抗肿瘤活性及机制研究进展[J].中国中药杂志,2015,40(3):373-381.
[17]孙晓润,陈苹苹,林悦,等.天然黄酮类化合物抗肿瘤作用靶点研究进展[J].中国实验方剂学杂志,2017(6):218-228.
[18]张彦兵,王天昶,朱娇,等.白花蛇舌草总黄酮对肝癌MHCC97-H细胞增殖的抑制作用[J].现代肿瘤医学,2015,23(19):2716-2719.
[19]谭玲玲,王忠,胡正海.桔梗中总黄酮的提取工艺优化[J].西北药学杂志,2014,29(06):568-570.
[20]孙乐,李春梅,郭迎迎,等.人参花中总皂苷的提取工艺优选[J].中国实验方剂学杂志,2015,21(01):21-23.
[21]卫亚丽,钟箫,汤洪敏.茜草中总蒽醌的提取工艺优化[J].江苏农业科学,2014,42(08):268-270.
[22]赵若琳,周坤福,张旭,等.桔梗皂苷-D抑制非小细胞肺癌H460和A549细胞黏附、侵袭和迁移的作用机制研究[J].中国药理学通报,2015(2):241-247.
[23]赵若琳,张旭,陈美娟,等.桔梗皂苷-D抗肿瘤机制研究进展[J].中国中医药信息杂志,2015,22(2):126-129.
[24]万小旭,张行,王佳贺.白花蛇舌草的有效成分2-羟基-3-甲基蒽醌通过Fas/FasL信号通路诱导卵巢癌细胞凋亡[J].实用药物与临床,2015,18(12):1405-1409.
[25]梁欣娜,张兴燊,滕红丽.中药及其有效成分抗肿瘤作用研究[J].时珍国医国药,2013,24(1):119-122.