基于因子分析与聚类分析评价补中益气汤对气虚发热大鼠免疫功能的影响
|
摘要
目的:探讨因子分析与聚类分析在综合评价补中益气汤(Buzhong Yiqi Decoction,BYD)对气虚发热大鼠免疫功能影响中的应用。方法:大鼠随机分成正常组、气虚发热组、阳性对照组(阿司匹林溶液0. 1 g·kg-1)、BYD高剂量组(25. 34 g·kg-1)、BYD中剂量组(12. 67 g·kg-1)、BYD低剂量组(6. 34 g·kg-1),在限食法的基础上采用番泻叶(0. 1 g·kg-1)苦寒泻下加游泳劳倦过度及注射脂多糖(LPS,80μg·kg-1)的方法建立气虚发热大鼠模型。造模42 d后连续给药6 d,末次给药后注射LPS,检测与免疫功能相关指标,包括CD4+、CD8+细胞百分比、补体C3水平、免疫球蛋白Ig M、Ig G水平及淋巴细胞转化率,并运用因子分析与聚类分析综合评价BYD对气虚发热大鼠免疫功能的影响。结果:BYD能显著改善气虚发热大鼠的免疫功能,其中低剂量效果最显著,中剂量与高剂量次之,阿司匹林效果较差。结论:因子分析与聚类分析对气虚发热大鼠的免疫功能进行综合评价是可行的,可作为评价中药复方疗效科学、客观的新方法。
OBJECTIVE To explore the application of factor analysis and cluster analysis in the comprehensive evaluation ofthe effect of Buzhong Yiqi Decoction( BYD) on immune function in rats with deficiency of vital energy and fever. METHODSRats were randomly divided into normal group,deficiency of vital energy and fever group,positive control group( aspirin solution0. 1 g·kg-1),BYD high dose group( 25. 34 g·kg-1),BYD medium dose group( 12. 67 g·kg-1),BYD low dose group(6. 34 g·kg-1). Deficiency of vital energy and fever model was established by combination of limiting diet,folium sennae bittercold diarrhea( 0. 1 g·kg-1),swimming over fatigue and injection of lipopolysaccharide( LPS,80 μg·kg-1) in rats. After themodel was established for 42 days,corresponding drug was administered continuously for 6 days. LPS was injected after the lastadministration,and then the immune function related indexes were detected,including the percentage of CD4+,CD8+cells,complement C3,immunoglobulin IgM,IgG level,and lymphocyte transformation rate. Finally,the effect of BYD on immunefunction in rats with deficiency of vital energy and fever was comprehensively evaluated by factor analysis and cluster analysis.RESULTS BYD significantly improved the immune function of the rats with deficiency of vital energy and fever,of which thelow dose effect was the most significant,the middle dose and the high dose were the next,and aspirin was relatively ineffective.CONCLUSION Factor analysis and cluster analysis are feasible for comprehensive evaluation of immune function in rats withdeficiency of vital energy and fever. It can be used as a scientific and objective method to evaluate the efficacy of traditional Chi-nese medicine compound prescriptions.
OBJECTIVE To explore the application of factor analysis and cluster analysis in the comprehensive evaluation ofthe effect of Buzhong Yiqi Decoction( BYD) on immune function in rats with deficiency of vital energy and fever. METHODSRats were randomly divided into normal group,deficiency of vital energy and fever group,positive control group( aspirin solution0. 1 g·kg-1),BYD high dose group( 25. 34 g·kg-1),BYD medium dose group( 12. 67 g·kg-1),BYD low dose group(6. 34 g·kg-1). Deficiency of vital energy and fever model was established by combination of limiting diet,folium sennae bittercold diarrhea( 0. 1 g·kg-1),swimming over fatigue and injection of lipopolysaccharide( LPS,80 μg·kg-1) in rats. After themodel was established for 42 days,corresponding drug was administered continuously for 6 days. LPS was injected after the lastadministration,and then the immune function related indexes were detected,including the percentage of CD4+,CD8+cells,complement C3,immunoglobulin IgM,IgG level,and lymphocyte transformation rate. Finally,the effect of BYD on immunefunction in rats with deficiency of vital energy and fever was comprehensively evaluated by factor analysis and cluster analysis.RESULTS BYD significantly improved the immune function of the rats with deficiency of vital energy and fever,of which thelow dose effect was the most significant,the middle dose and the high dose were the next,and aspirin was relatively ineffective.CONCLUSION Factor analysis and cluster analysis are feasible for comprehensive evaluation of immune function in rats withdeficiency of vital energy and fever. It can be used as a scientific and objective method to evaluate the efficacy of traditional Chi-nese medicine compound prescriptions.
引文
[1] Ruan Y,Song SJ,Liu L. New explanation of"deficiency of vitalenergy and fever"[J]. Lishizhen Med Mat Med Res(时珍国医国药),2017,28(3):668-670.
[2] Zhang L,Chopp M,Zhang Y,et al. Diabetes Mellitus ImpairsCognitive Function in Middle-Aged Rats and Neurological Recover-y in Middle-Aged Rats After Stroke[J]. Stroke,2016,47(8):2112-2118.
[3] Wang YE,Xi YY,Li Y,et al. Compatibility Discipline ofBuzhong Yiqi tang[J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志),2017,23(7):221-226.
[4] Gregory TF,Rennels ML,Blaumanis OR,et al. A method for mi-croscopic studies of cerebral angioarchitecture and vascular-paren-chymal relationships,based on the demonstration of'paravascular'fluid pathways in the mammalian central nervous system[J]. JNeurosci Methods,1985,14(1):5-14.
[5] Nielsen-Kudsk F. A pharmacokinetic program for TI-59[J]. JPharmacol Methods,1980,3(4):345-355.
[6] Zang LM. Main pharmacological effects and research progress ofBuzhong Yiqi Decoction[J]. Zhejiang Animal Husb Veterin Med(浙江畜牧兽医),2014,39(3):18-19.
[7] Wang H,Chen YF,Shi J,et al. Methodology on skin externalmedicine[M]. First edition. Beijing:People's Medical Publish-ing House(北京:人民卫生出版社),2017.
[8] Guo QJ,Chang YM,Li JS,et al. Exhaustive Standard on Swim-ming Fatigue Model in Rats[J]. Prog Mod Biomed(现代生物医学进展),2010,10(15):2855-2858.
[9] Deng MY,Wang H,Tang XF,et al. Discussing the effect ofBuzhong Yiqi Decoction on temperature changes of rat based on se-quence forecast theory of grey systems[J]. Chin J Tradit chinMed Pharm(中华中医药杂志),2011,26(9):2107-2111.
[10] Wang Y,Wen L,Chen MF. Dictionary of mathematics[M].Beijing:The Science Publishing Company(科学出版社),2010:112-118.
[11] Watson R,Thompson DR. Use of factor analysis in Journal of Ad-vanced Nursing:literature review[J]. J Adv Nurs,2006,55(3):330-341.
[12] Semmar N,Bruguerolle B,Boulluciocca S,et al. Cluster analy-sis:an alternative method for covariate selection in populationpharmacokinetic modeling[J]. J Pharmacokinet Pharmacodyn,2005,32(3-4):333-358.
[13] Lei D,Holder RL,Smith FW,et al. Cluster analysis as a methodfor determining size ranges for spinal implants:disc lumbar re-placement prosthesis dimensions from magnetic resonance images[J]. Spine(Phila Pa 1976),2006,31(25):2979-2993.
[14] Deng CY,Liu HB,Wang H. Application of weighted Topsismethod in the evaluation of transdermal enhancer effect[J]. ChinJ Clin Pharmacol Therap(中国临床药理学与治疗学),2016,21(4):412-416.
[15] Barbiers M,Timmermans JP,Adriaensen D,et al. Projections ofneurochemically specified neurons in the porcine colon[J]. His-tochem Cell Biol,1995,103(2):115-126.
[16] Borroto-Escuela DO,Agnati LF,Bechter K,et al. The role oftransmitter diffusion and flow versus extracellular vesicles in vol-ume transmission in the brain neural-glial networks[J]. PhilosTrans R Soc Lond B Biol Sci,2015,370(1672):1-14.
[17] Jia B,Li Y. Pharmacology of traditional Chinese medical formulae[M]. Beijing:China Press of Traditional Chinese Medicine(中国中医药出版社),2011:232-234.
[18] Liang YB,Liang LP,Li JL,et al. Effects of Buzhongyiqi Decoc-tion on cellular immune function of rats with spleen-qi deficiencywith fever[J]. Pharmacol Clin Pharmacol Tradit Chin Med(中药药理与临床),2017,33(3):18-22.
[19] Wang CM,Zhang LF,Guan RH,et al. Effect of administration ofPHA(phytohemagglutinin)by different ways on lymphocyte trans-formation rate of kunming strain mouse[J]. Mod Prevent Med(现代预防医学),2012,39(1):107-109.
[20] Yu SM,Gan M,Zuo ZC,et al. Effects of Chinese herbal com-pound on cytokines,immunoglobulins and erythrocyte immune ad-hesion function of immunosuppressive mice[J]. J Northwest A FUniversity(西北农林科技大学学报:自然科学版),2014,42(4):27-32.
[21] Liang LP,Liang YB,Li JL,et al. Effects of Buzhongyiqi Decoc-tion on humoral immune function of rats with spleen-qi deficiencywith fever[J]. Chin Hosp Pharm J(中国医院药学杂志),2017,37(13):1224-1227.
[22] Pihlgren M,Fulurija A,Villiers MB,et al. Influence of comple-ment C3 amount on Ig G responses in early life:immunization withC3b-conjugated antigen increases murine neonatal antibody respon-ses[J]. Vaccine,2005,23(3):329-335.
[23] Zhu XF,Xie KP,Huo HN,et al. Antibacterial function of liquo-rice extracts and its influence on the mice immune functions[J].Chin J Microecol(中国微生态学杂志),2013,25(3):254-257.
[2] Zhang L,Chopp M,Zhang Y,et al. Diabetes Mellitus ImpairsCognitive Function in Middle-Aged Rats and Neurological Recover-y in Middle-Aged Rats After Stroke[J]. Stroke,2016,47(8):2112-2118.
[3] Wang YE,Xi YY,Li Y,et al. Compatibility Discipline ofBuzhong Yiqi tang[J]. Chin J Exp Tradit Med Form(中国实验方剂学杂志),2017,23(7):221-226.
[4] Gregory TF,Rennels ML,Blaumanis OR,et al. A method for mi-croscopic studies of cerebral angioarchitecture and vascular-paren-chymal relationships,based on the demonstration of'paravascular'fluid pathways in the mammalian central nervous system[J]. JNeurosci Methods,1985,14(1):5-14.
[5] Nielsen-Kudsk F. A pharmacokinetic program for TI-59[J]. JPharmacol Methods,1980,3(4):345-355.
[6] Zang LM. Main pharmacological effects and research progress ofBuzhong Yiqi Decoction[J]. Zhejiang Animal Husb Veterin Med(浙江畜牧兽医),2014,39(3):18-19.
[7] Wang H,Chen YF,Shi J,et al. Methodology on skin externalmedicine[M]. First edition. Beijing:People's Medical Publish-ing House(北京:人民卫生出版社),2017.
[8] Guo QJ,Chang YM,Li JS,et al. Exhaustive Standard on Swim-ming Fatigue Model in Rats[J]. Prog Mod Biomed(现代生物医学进展),2010,10(15):2855-2858.
[9] Deng MY,Wang H,Tang XF,et al. Discussing the effect ofBuzhong Yiqi Decoction on temperature changes of rat based on se-quence forecast theory of grey systems[J]. Chin J Tradit chinMed Pharm(中华中医药杂志),2011,26(9):2107-2111.
[10] Wang Y,Wen L,Chen MF. Dictionary of mathematics[M].Beijing:The Science Publishing Company(科学出版社),2010:112-118.
[11] Watson R,Thompson DR. Use of factor analysis in Journal of Ad-vanced Nursing:literature review[J]. J Adv Nurs,2006,55(3):330-341.
[12] Semmar N,Bruguerolle B,Boulluciocca S,et al. Cluster analy-sis:an alternative method for covariate selection in populationpharmacokinetic modeling[J]. J Pharmacokinet Pharmacodyn,2005,32(3-4):333-358.
[13] Lei D,Holder RL,Smith FW,et al. Cluster analysis as a methodfor determining size ranges for spinal implants:disc lumbar re-placement prosthesis dimensions from magnetic resonance images[J]. Spine(Phila Pa 1976),2006,31(25):2979-2993.
[14] Deng CY,Liu HB,Wang H. Application of weighted Topsismethod in the evaluation of transdermal enhancer effect[J]. ChinJ Clin Pharmacol Therap(中国临床药理学与治疗学),2016,21(4):412-416.
[15] Barbiers M,Timmermans JP,Adriaensen D,et al. Projections ofneurochemically specified neurons in the porcine colon[J]. His-tochem Cell Biol,1995,103(2):115-126.
[16] Borroto-Escuela DO,Agnati LF,Bechter K,et al. The role oftransmitter diffusion and flow versus extracellular vesicles in vol-ume transmission in the brain neural-glial networks[J]. PhilosTrans R Soc Lond B Biol Sci,2015,370(1672):1-14.
[17] Jia B,Li Y. Pharmacology of traditional Chinese medical formulae[M]. Beijing:China Press of Traditional Chinese Medicine(中国中医药出版社),2011:232-234.
[18] Liang YB,Liang LP,Li JL,et al. Effects of Buzhongyiqi Decoc-tion on cellular immune function of rats with spleen-qi deficiencywith fever[J]. Pharmacol Clin Pharmacol Tradit Chin Med(中药药理与临床),2017,33(3):18-22.
[19] Wang CM,Zhang LF,Guan RH,et al. Effect of administration ofPHA(phytohemagglutinin)by different ways on lymphocyte trans-formation rate of kunming strain mouse[J]. Mod Prevent Med(现代预防医学),2012,39(1):107-109.
[20] Yu SM,Gan M,Zuo ZC,et al. Effects of Chinese herbal com-pound on cytokines,immunoglobulins and erythrocyte immune ad-hesion function of immunosuppressive mice[J]. J Northwest A FUniversity(西北农林科技大学学报:自然科学版),2014,42(4):27-32.
[21] Liang LP,Liang YB,Li JL,et al. Effects of Buzhongyiqi Decoc-tion on humoral immune function of rats with spleen-qi deficiencywith fever[J]. Chin Hosp Pharm J(中国医院药学杂志),2017,37(13):1224-1227.
[22] Pihlgren M,Fulurija A,Villiers MB,et al. Influence of comple-ment C3 amount on Ig G responses in early life:immunization withC3b-conjugated antigen increases murine neonatal antibody respon-ses[J]. Vaccine,2005,23(3):329-335.
[23] Zhu XF,Xie KP,Huo HN,et al. Antibacterial function of liquo-rice extracts and its influence on the mice immune functions[J].Chin J Microecol(中国微生态学杂志),2013,25(3):254-257.