食源性抗凝血肽的活性作用机制及检测方法研究进展
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摘要
近年来,已从各类食源性蛋白中分离鉴定出抗凝血生物活性肽。食源性抗凝血肽的活性机制主要集中在抑制凝血系统中的凝血酶的活性、除凝血酶外的其它凝血因子的活性及血小板聚集三个方面。然而,体外抗凝血活性的主要分析方法如:分光光度法、发色底物法、活化部分凝血活酶时间测定、凝血酶时间测定、凝血酶原时间测定、血小板聚集分析等均有各自优缺点。本文将对抗凝血肽的活性机制及相应的抗凝血检测分析方法进行总结分析,以期为相关研究提供理论基础。
In recent years,anticoagulant bioactive peptides have been isolated and identified from various food-derived proteins. The active mechanisms of food-derived anticoagulant peptides were mainly focused on three aspects:inhibition of thrombin,the coagulation factors other than thrombin,and platelet aggregation. However,the main in vitro analytical methods for verifying anticoagulant activity,such as spectrophotometry,chromogenic substrate method,activated partial thromboplastin time testing,thrombin time testing,prothrombin time testing,platelet aggregation assay are with both advantages and disadvantages. The active mechanisms of anticoagulant peptides and the corresponding detection methods were analyzed and summarized in this paper and to provide theoretical basis for the related research.
In recent years,anticoagulant bioactive peptides have been isolated and identified from various food-derived proteins. The active mechanisms of food-derived anticoagulant peptides were mainly focused on three aspects:inhibition of thrombin,the coagulation factors other than thrombin,and platelet aggregation. However,the main in vitro analytical methods for verifying anticoagulant activity,such as spectrophotometry,chromogenic substrate method,activated partial thromboplastin time testing,thrombin time testing,prothrombin time testing,platelet aggregation assay are with both advantages and disadvantages. The active mechanisms of anticoagulant peptides and the corresponding detection methods were analyzed and summarized in this paper and to provide theoretical basis for the related research.
引文
[1]Deshwal S,Di SM,Di LF,et al. Emerging role of monoamine oxidase as a therapeutic target for cardiovascular disease[J]. Curr Opin Pharm,2017,33:64-69.
[2]Tu M,Liu H,Zhang R,et al. Analysis and evaluation of the inhibitory mechanism of a novel angiotensin-i-converting enzyme inhibitory peptide derived from casein hydrolysate[J]. J Agric Food Chem,2018,66(16):4139-4144.
[3]Phelan M,Kerins D. The potential role of milk-derived peptides in cardiovascular disease[J]. Food & Function,2011,2(3-4):153-167.
[4]Wang L,Zhang H-L,Lu R,et al. The decapeptide cms001 enhances swimming endurance in mice[J]. Peptides,2008,29(7):1176-1182.
[5]Shoyele SA,Slowey A. Prospects of formulating proteins/peptides as aerosols for pulmonary drug delivery[J]. Int J Pharm,2006,314(1):1-8.
[6]Udenigwe CC,Aluko RE. Food protein-derived bioactive peptides:Production,processing,and potential health benefits[J].J Food Sci,2012,77(1):R11-R24.
[7]Rojas-Ronquillo R,Cruz-Guerrero A,Flores-Nájera A,et al. Antithrombotic and angiotensin-converting enzyme inhibitory properties of peptides released from bovine casein by Lactobacillus casei shirota[J]. International Dairy Journal,2012,26(2):147-154.
[8]Yu Z,Yin Y,Zhao W,et al. Characterization of ace-inhibitory peptide associated with antioxidant and anticoagulation properties[J]. J Food Sci,2011,76(8):C1149-C1155.
[9]Jung W-K,Kim S-K. Isolation and characterisation of an anticoagulant oligopeptide from blue mussel,mytilus edulis[J]. Food Chem,2009,117(4):687-692.
[10]Nasri R,Nasri M. Marine-derived bioactive peptides as new anticoagulant agents:A review[J]. Curr Protein Pept Sci,2013,14(3):199-204.
[11]Zarpellon A,Celikel R,Roberts JR,et al. Binding of α-thrombin to surface-anchored platelet glycoprotein ibα sulfotyrosines through a two-site mechanism involving exosite i[J]. Proceedings of the National Academy of Sciences,2011,108(21):8628-8633.
[12]Chahal G,Thorpe M,Hellman L. The importance of exosite interactions for substrate cleavage by human thrombin[J]. PloS One,2015,10(6):e0129511.
[13]Adams TE,Huntington JA. Thrombin-cofactor interactions:Structural insights into regulatory mechanisms[J]. Arteriosclerosis Thrombosis & Vascular Biology,2006,26(8):1738-1745.
[14]Bode W. Structure and interaction modes of thrombin[J]. Blood Cells Molecules & Diseases,2006,36(2):122-130.
[15]许善峰,李芳,姜涌明,等. 凝血酶的结构及其变构特性[J]. 中国医学生物技术应用,2004(3):18-23.
[16]Kikelj D. Peptidomimetic thrombin inhibitors[J]. Pathophysiol Haemost Thromb,2003,33(5-6):487-491.
[17]Cappello M,Bergum PW,Vlasuk GP,et al. Isolation and characterization of the tsetse thrombin inhibitor:A potent antithrombotic peptide from the saliva of glossina morsitans morsitans[J]. The American Journal of Tropical Medicine and Hygiene,1996,54(5):475-480.
[18]Khiari Z,Rico D,Martin-Diana AB,et al. Structure elucidation of ace-inhibitory and antithrombotic peptides isolated from mackerel skin gelatine hydrolysates[J]. J Sci Food Agric,2014,94(8):1663-1671.
[19]Chalé FH,Ruiz JCR,Ancona DB,et al. The hypolipidemic effect and antithrombotic activity of mucuna pruriens protein hydrolysates[J]. Food & Function,2016,7(1):434-444.
[20]Qiao M,Tu M,Wang Z,et al. Identification and antithrombotic activity of peptides from blue mussel(mytilus edulis)protein[J]. International Journal of Molecular Sciences,2018,19(1):138.
[21]Palta S,Saroa R,Palta A. Overview of the coagulation system[J]. Indian J Anaesth,2014,58(5):515-523.
[22]Waxman L,Smith DE,Arcuri KE,et al. Tick anticoagulant peptide(tap)is a novel inhibitor of blood coagulation factor xa[J].Sci,1990,248(4955):593-596.
[23]Kong Y,Shao Y,Chen H,et al. A novel factor xa-inhibiting peptide from centipedes venom[J]. Int J Pept Res Ther,2013,19(4):303-311.
[24]Almhanawi BH,Khalid B,Ibrahim TA,et al. A transmission electron microscopy study of anticoagulant-induced platelet vesiculation[J]. Porto Biomedical Journal,2017,2(1):23-27.
[25]Linden M,Ward JM,Cherian S. 19 - hematopoietic and lymphoid tissues[J]. Comparative Anatomy & Histology,2012:309-338.
[26]Budnik I,Shenkman B,Hauschner H,et al. Role of heterotrimeric g proteins in platelet activation and clot formation in platelets treated with integrin αiibβ3 inhibitor[J]. Platelets,2017,29(3):265-269.
[27]Beck F,Geiger J,Gambaryan S,et al. Temporal quantitative phosphoproteomics of adp stimulation reveals novel central nodes in platelet activation and inhibition[J]. Blood,2017,129(2):e1-e12.
[28]刘刚. 白杨素抗血小板及抗血栓作用的研究[D]. 武汉:华中科技大学,2014.
[29]Wang S,Jiang J,Wang Y,et al. Rlj-rgd3,a novel recombinant toxin protein from lampetra japonica,prevents coronary thrombosis-induced acute myocardial infarction by inhibiting platelet functions in rats[J]. Biochem Biophys Res Commun,2018,498(1):240-245.
[30]Jollès P,Lévy-toledano S,Fiat AM,et al. Analogy between fibrinogen and casein:Effect of an undecapeptide isolated from k-casein on platelet function[J]. European Journal of Biochemistry,1986,158(2):379-382.
[31]Kong Y,Li S,Shao Y,et al. Antithrombotic peptides from scolopendra subspinipes mutilans hydrolysates[J]. International Journal of Peptide Research and Therapeutics,2014,20(2):245-252.
[32]Lee K-A,Kim S-H. Ssge and dee,new peptides isolated from a soy protein hydrolysate that inhibit platelet aggregation[J].Food Chem,2005,90(3):389-393.
[33]Yang,Zhang,Wang,et al. A new method for determination of antithrombotic activity of egg white protein hydrolysate by microplate reader[J]. 中国化学快报(英文版),2007,18(4):449-451.
[34]Feng L,Tu M,Qiao M,et al. Thrombin inhibitory peptides derived from mytilus edulis proteins:Identification,molecular docking and in silico prediction of toxicity[J]. European Food Research and Technology,2018,244(2):207-217.
[35]Tu M,Feng L,Wang Z,et al. Sequence analysis and molecular docking of antithrombotic peptides from casein hydrolysate by trypsin digestion[J]. Journal of Functional Foods,2017,32:313-323.
[36]Ren Y,Wu H,Lai F,et al. Isolation and identification of a novel anticoagulant peptide from enzymatic hydrolysates of scorpion(buthus martensii karsch)protein[J]. Food Research International,2014,64:931-938.
[37]Yu Z,Yin Y,Zhao W,et al. Characterization of ace-inhibitory peptide associated with antioxidant and anticoagulation properties[J]. J Food Sci,2011,76(8):C1149-C1155.
[38]Motoyashiki T,Tu AT,Azimov DA,et al. Isolation of anticoagulant from the venom of tick,boophilus calcaratus,from uzbekistan[J]. Thromb Res,2003,110(4):235-241.
[39]Cleary DB,Ehringer WD,Maurer MC. Establishing the inhibitory effects of bradykinin on thrombin[J]. Arch Biochem Biophys,2003,410(1):96-106.
[40]Francischetti IM,Valenzuela JG,Ribeiro JM. Anophelin:Kinetics and mechanism of thrombin inhibition[J]. Biochemistry,1999,38(50):16678-16685.
[41]Nasri R,Amor IB,Bougatef A,et al. Anticoagulant activities of goby muscle protein hydrolysates[J]. Food Chem,2012,133(3):835-841.
[42]Indumathi P,Mehta A. A novel anticoagulant peptide from the nori hydrolysate[J]. Journal of Functional Foods,2016,20:606-617.
[43]Besharati S,Khodabandeh S. Anticoagulant properties of protein hydrolysates from the muscle of sea cucumber[J]. Journal of Mazandaran University of Medical Sciences,2017,26(145):371-376.
[44]Li D,He Q,Kang T,et al. Identification of an anticoagulant peptide that inhibits both fxia and fviia/tissue factor from the blood-feeding nematode ancylostoma caninum[J]. Biochem Biophys Res Commun,2010,392(2):155-159.
[45]Gan W,Deng L,Yang C,et al. An anticoagulant peptide from the human hookworm,ancylostoma duodenale that inhibits coagulation factors xa and xia[J]. Febs Letters,2009,583(12):1976-1980.
[46]Qian ZY,Jollès P,Miglioresamour D,et al. Sheep kappa-casein peptides inhibit platelet aggregation[J]. AcBB,1995,1244(2-3):411-417.
[47]Silva MCC,Santana LA,Silva-Lucca RA,et al. Immobilized cratylia mollis lectin:An affinity matrix to purify a soybean(glycine max)seed protein with in vitro platelet antiaggregation and anticoagulant activities[J]. Process Biochemistry,2011,46(1):74-80.
[48]Clare DA,Swaisgood HE. Bioactive milk peptides:A prospectus[J]. J Dairy Sci,2000,83(6):1187-1195.
[49]Bal dit Sollier C,Drouet L,Pignaud G,et al. Effect of κ-casein split peptides on platelet aggregation and on thrombus formation in the guinea-pig[J]. Thromb Res,1996,81(4):427-437.
[50]Kong Y,Huang SL,Shao Y,et al. Purification and characterization of a novel antithrombotic peptide from scolopendra subspinipes mutilans[J]. Journal of Ethnopharmacology,2013,145(1):182-186.
[51]Kong Y,Xu C,He ZL,et al. A novel peptide inhibitor of platelet aggregation from stiff silkworm,bombyx batryticatus[J]. Peptides,2014,53:70-78.
[2]Tu M,Liu H,Zhang R,et al. Analysis and evaluation of the inhibitory mechanism of a novel angiotensin-i-converting enzyme inhibitory peptide derived from casein hydrolysate[J]. J Agric Food Chem,2018,66(16):4139-4144.
[3]Phelan M,Kerins D. The potential role of milk-derived peptides in cardiovascular disease[J]. Food & Function,2011,2(3-4):153-167.
[4]Wang L,Zhang H-L,Lu R,et al. The decapeptide cms001 enhances swimming endurance in mice[J]. Peptides,2008,29(7):1176-1182.
[5]Shoyele SA,Slowey A. Prospects of formulating proteins/peptides as aerosols for pulmonary drug delivery[J]. Int J Pharm,2006,314(1):1-8.
[6]Udenigwe CC,Aluko RE. Food protein-derived bioactive peptides:Production,processing,and potential health benefits[J].J Food Sci,2012,77(1):R11-R24.
[7]Rojas-Ronquillo R,Cruz-Guerrero A,Flores-Nájera A,et al. Antithrombotic and angiotensin-converting enzyme inhibitory properties of peptides released from bovine casein by Lactobacillus casei shirota[J]. International Dairy Journal,2012,26(2):147-154.
[8]Yu Z,Yin Y,Zhao W,et al. Characterization of ace-inhibitory peptide associated with antioxidant and anticoagulation properties[J]. J Food Sci,2011,76(8):C1149-C1155.
[9]Jung W-K,Kim S-K. Isolation and characterisation of an anticoagulant oligopeptide from blue mussel,mytilus edulis[J]. Food Chem,2009,117(4):687-692.
[10]Nasri R,Nasri M. Marine-derived bioactive peptides as new anticoagulant agents:A review[J]. Curr Protein Pept Sci,2013,14(3):199-204.
[11]Zarpellon A,Celikel R,Roberts JR,et al. Binding of α-thrombin to surface-anchored platelet glycoprotein ibα sulfotyrosines through a two-site mechanism involving exosite i[J]. Proceedings of the National Academy of Sciences,2011,108(21):8628-8633.
[12]Chahal G,Thorpe M,Hellman L. The importance of exosite interactions for substrate cleavage by human thrombin[J]. PloS One,2015,10(6):e0129511.
[13]Adams TE,Huntington JA. Thrombin-cofactor interactions:Structural insights into regulatory mechanisms[J]. Arteriosclerosis Thrombosis & Vascular Biology,2006,26(8):1738-1745.
[14]Bode W. Structure and interaction modes of thrombin[J]. Blood Cells Molecules & Diseases,2006,36(2):122-130.
[15]许善峰,李芳,姜涌明,等. 凝血酶的结构及其变构特性[J]. 中国医学生物技术应用,2004(3):18-23.
[16]Kikelj D. Peptidomimetic thrombin inhibitors[J]. Pathophysiol Haemost Thromb,2003,33(5-6):487-491.
[17]Cappello M,Bergum PW,Vlasuk GP,et al. Isolation and characterization of the tsetse thrombin inhibitor:A potent antithrombotic peptide from the saliva of glossina morsitans morsitans[J]. The American Journal of Tropical Medicine and Hygiene,1996,54(5):475-480.
[18]Khiari Z,Rico D,Martin-Diana AB,et al. Structure elucidation of ace-inhibitory and antithrombotic peptides isolated from mackerel skin gelatine hydrolysates[J]. J Sci Food Agric,2014,94(8):1663-1671.
[19]Chalé FH,Ruiz JCR,Ancona DB,et al. The hypolipidemic effect and antithrombotic activity of mucuna pruriens protein hydrolysates[J]. Food & Function,2016,7(1):434-444.
[20]Qiao M,Tu M,Wang Z,et al. Identification and antithrombotic activity of peptides from blue mussel(mytilus edulis)protein[J]. International Journal of Molecular Sciences,2018,19(1):138.
[21]Palta S,Saroa R,Palta A. Overview of the coagulation system[J]. Indian J Anaesth,2014,58(5):515-523.
[22]Waxman L,Smith DE,Arcuri KE,et al. Tick anticoagulant peptide(tap)is a novel inhibitor of blood coagulation factor xa[J].Sci,1990,248(4955):593-596.
[23]Kong Y,Shao Y,Chen H,et al. A novel factor xa-inhibiting peptide from centipedes venom[J]. Int J Pept Res Ther,2013,19(4):303-311.
[24]Almhanawi BH,Khalid B,Ibrahim TA,et al. A transmission electron microscopy study of anticoagulant-induced platelet vesiculation[J]. Porto Biomedical Journal,2017,2(1):23-27.
[25]Linden M,Ward JM,Cherian S. 19 - hematopoietic and lymphoid tissues[J]. Comparative Anatomy & Histology,2012:309-338.
[26]Budnik I,Shenkman B,Hauschner H,et al. Role of heterotrimeric g proteins in platelet activation and clot formation in platelets treated with integrin αiibβ3 inhibitor[J]. Platelets,2017,29(3):265-269.
[27]Beck F,Geiger J,Gambaryan S,et al. Temporal quantitative phosphoproteomics of adp stimulation reveals novel central nodes in platelet activation and inhibition[J]. Blood,2017,129(2):e1-e12.
[28]刘刚. 白杨素抗血小板及抗血栓作用的研究[D]. 武汉:华中科技大学,2014.
[29]Wang S,Jiang J,Wang Y,et al. Rlj-rgd3,a novel recombinant toxin protein from lampetra japonica,prevents coronary thrombosis-induced acute myocardial infarction by inhibiting platelet functions in rats[J]. Biochem Biophys Res Commun,2018,498(1):240-245.
[30]Jollès P,Lévy-toledano S,Fiat AM,et al. Analogy between fibrinogen and casein:Effect of an undecapeptide isolated from k-casein on platelet function[J]. European Journal of Biochemistry,1986,158(2):379-382.
[31]Kong Y,Li S,Shao Y,et al. Antithrombotic peptides from scolopendra subspinipes mutilans hydrolysates[J]. International Journal of Peptide Research and Therapeutics,2014,20(2):245-252.
[32]Lee K-A,Kim S-H. Ssge and dee,new peptides isolated from a soy protein hydrolysate that inhibit platelet aggregation[J].Food Chem,2005,90(3):389-393.
[33]Yang,Zhang,Wang,et al. A new method for determination of antithrombotic activity of egg white protein hydrolysate by microplate reader[J]. 中国化学快报(英文版),2007,18(4):449-451.
[34]Feng L,Tu M,Qiao M,et al. Thrombin inhibitory peptides derived from mytilus edulis proteins:Identification,molecular docking and in silico prediction of toxicity[J]. European Food Research and Technology,2018,244(2):207-217.
[35]Tu M,Feng L,Wang Z,et al. Sequence analysis and molecular docking of antithrombotic peptides from casein hydrolysate by trypsin digestion[J]. Journal of Functional Foods,2017,32:313-323.
[36]Ren Y,Wu H,Lai F,et al. Isolation and identification of a novel anticoagulant peptide from enzymatic hydrolysates of scorpion(buthus martensii karsch)protein[J]. Food Research International,2014,64:931-938.
[37]Yu Z,Yin Y,Zhao W,et al. Characterization of ace-inhibitory peptide associated with antioxidant and anticoagulation properties[J]. J Food Sci,2011,76(8):C1149-C1155.
[38]Motoyashiki T,Tu AT,Azimov DA,et al. Isolation of anticoagulant from the venom of tick,boophilus calcaratus,from uzbekistan[J]. Thromb Res,2003,110(4):235-241.
[39]Cleary DB,Ehringer WD,Maurer MC. Establishing the inhibitory effects of bradykinin on thrombin[J]. Arch Biochem Biophys,2003,410(1):96-106.
[40]Francischetti IM,Valenzuela JG,Ribeiro JM. Anophelin:Kinetics and mechanism of thrombin inhibition[J]. Biochemistry,1999,38(50):16678-16685.
[41]Nasri R,Amor IB,Bougatef A,et al. Anticoagulant activities of goby muscle protein hydrolysates[J]. Food Chem,2012,133(3):835-841.
[42]Indumathi P,Mehta A. A novel anticoagulant peptide from the nori hydrolysate[J]. Journal of Functional Foods,2016,20:606-617.
[43]Besharati S,Khodabandeh S. Anticoagulant properties of protein hydrolysates from the muscle of sea cucumber[J]. Journal of Mazandaran University of Medical Sciences,2017,26(145):371-376.
[44]Li D,He Q,Kang T,et al. Identification of an anticoagulant peptide that inhibits both fxia and fviia/tissue factor from the blood-feeding nematode ancylostoma caninum[J]. Biochem Biophys Res Commun,2010,392(2):155-159.
[45]Gan W,Deng L,Yang C,et al. An anticoagulant peptide from the human hookworm,ancylostoma duodenale that inhibits coagulation factors xa and xia[J]. Febs Letters,2009,583(12):1976-1980.
[46]Qian ZY,Jollès P,Miglioresamour D,et al. Sheep kappa-casein peptides inhibit platelet aggregation[J]. AcBB,1995,1244(2-3):411-417.
[47]Silva MCC,Santana LA,Silva-Lucca RA,et al. Immobilized cratylia mollis lectin:An affinity matrix to purify a soybean(glycine max)seed protein with in vitro platelet antiaggregation and anticoagulant activities[J]. Process Biochemistry,2011,46(1):74-80.
[48]Clare DA,Swaisgood HE. Bioactive milk peptides:A prospectus[J]. J Dairy Sci,2000,83(6):1187-1195.
[49]Bal dit Sollier C,Drouet L,Pignaud G,et al. Effect of κ-casein split peptides on platelet aggregation and on thrombus formation in the guinea-pig[J]. Thromb Res,1996,81(4):427-437.
[50]Kong Y,Huang SL,Shao Y,et al. Purification and characterization of a novel antithrombotic peptide from scolopendra subspinipes mutilans[J]. Journal of Ethnopharmacology,2013,145(1):182-186.
[51]Kong Y,Xu C,He ZL,et al. A novel peptide inhibitor of platelet aggregation from stiff silkworm,bombyx batryticatus[J]. Peptides,2014,53:70-78.