加工方法对金银花质量的影响
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摘要
目的:研究不同加工方法对金银花中绿原酸、木犀草苷和氨基酸含量的影响。方法:分别采用高效液相色谱法和GB/T 8314—2002茶-游离氨基酸测定不同加工方法处理的金银花中绿原酸、木犀草苷和氨基酸含量,记录金银花的外观、水浸出物、酸不溶性灰分和总灰分。结果:微波杀青后低温干燥的方法具有良好的外观性状,经微波杀青低温干燥处理后,绿原酸和木犀草苷的含量较高,氨基酸含量降低,绿原酸、木犀草苷和氨基酸含量之和最高(P <0. 05),微波杀青低温干燥的总灰分均较低(P <0. 05)。结论:微波杀青低温干燥法是合适的金银花加工方法,值得在种植区推广。
Objective: To study the effects of different processing methods on the content of chlorogenic acid,luteolin and amino acids in Lonicerae Flos. Methods: The high performance liquid chromatography and GB/T8314-2002 tea-free amino acid were used to determine the content of chlorogenic acid,luteolin and amino acid in Flos Lonicerae treated by different processing methods. The appearance,water extract,acid-insoluble and total ash of Lonicerae Flos were recorded. Results: The method of the low temperature drying after microwave sterilization had good appearance. After microwave drying and low temperature drying treatment,the contents of chlorogenic acid and luteolin increased,the amino acid content decreased,and the sum of chlorogenic acid,luteolin and amino acid content was the highest( P < 0. 05). The total ash of Lonicerae Flos treated by low temperature drying after microwave sterilization decreased( P < 0. 05). Conclusion: The low temperature drying after microwave sterilization method is a suitable method for processing Lonicerae Flos,which is worthy of promotion in Lonicerae Flos planting areas.
Objective: To study the effects of different processing methods on the content of chlorogenic acid,luteolin and amino acids in Lonicerae Flos. Methods: The high performance liquid chromatography and GB/T8314-2002 tea-free amino acid were used to determine the content of chlorogenic acid,luteolin and amino acid in Flos Lonicerae treated by different processing methods. The appearance,water extract,acid-insoluble and total ash of Lonicerae Flos were recorded. Results: The method of the low temperature drying after microwave sterilization had good appearance. After microwave drying and low temperature drying treatment,the contents of chlorogenic acid and luteolin increased,the amino acid content decreased,and the sum of chlorogenic acid,luteolin and amino acid content was the highest( P < 0. 05). The total ash of Lonicerae Flos treated by low temperature drying after microwave sterilization decreased( P < 0. 05). Conclusion: The low temperature drying after microwave sterilization method is a suitable method for processing Lonicerae Flos,which is worthy of promotion in Lonicerae Flos planting areas.
引文
[1] KWON Seung-Hwan,MA Shi-Xun,HONG Sa-Ik,et al. Lonicera japonica Thunb. Extract Inhibits LipopolysaccharideStimulated Inflammatory Responses by Suppressing NF-κB Signaling in BV-2 Microglial Cells[J]. J Med Food,2015,18(7):762-775.
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[9]王永香,罗勇,沈娟,等.不同产地加工方法对江苏省东海县种植基地金银花质量影响的研究[J].中国中药杂志,2014,39(14):2665-2669.
[10]宋健,张会敏,石俊英.金银花最佳产地加工方法——杀青烘干干燥法[J].中药材,2008,31(4):489-491.
[11]武琳,黄菲,杨光.复方罗布麻颗粒中木犀草苷含量测定方法研究[J].中国中医药信息杂志,2015,22(4):91-93
[12]王川丕,刘新,诸力.两种方法测定茶叶游离氨基酸总量的差异及其原因分析[J].中国茶叶,2012,34(12):12-14.
[13]陈燕文,王玲娜,梁从莲,等.干燥方法对金银花多糖含量的影响[J].中国现代中药,2017,19(1):88-91.
[14] RAI Amit,KAMOCHI Hidetaka,SUZUKI Hideyuki,et al.De novo transcriptome assembly and characterization of nine tissues of Lonicera japonica to identify potential candidate genes involved in chlorogenic acid,luteolosides,and secoiridoid biosynthesis pathways[J]. J Nat Med,2017,71(1):1-15.
[15] CHAOWUTTIKUL C,PALANUVEJ C,RUANGRUNGSI N.Pharmacognostic Specification,Chlorogenic Acid Content,and In vitro Antioxidant Activities of Lonicera japonica Flowering Bud[J]. Pharmacognosy Res,2017,9(2):128-132.
[16]马静遥,刘新民,金哲雄,等.改善认知功能障碍中药研究进展[J].中国中医药信息杂志,2013,20(9):104-107.
[17] WU Jie,WANG Xiao-Chen,LIU Yang,et al. Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation[J]. Sci Rep,2016,6(1):19245.
[18]谷民举,刘明岭,王琰.金银花质量与产地影响因素相关性研究[J].药学研究,2018,37(5):295-297.
[19]刘涛,王鸿,李青苗,等.不同采收期金银花的产地加工方法考察[J].中国实验方剂学杂志,2016,22(10):28-31.
[20]祁丹丹,戴伟东,谭俊峰,等.杀青方式对夏季绿茶化学成分及滋味品质的影响[J].茶叶科学,2016,36(1):18-26.
[21]崔文甲,曹世宁,王文亮,等.微波辅助提取金针菇、香菇菇柄中氨基酸的研究[J].食品工业,2018,39(9):96-99.
[2] PARK C,LEE W S,HAN M H,et al. Lonicera japonica Thunb. Induces caspase-dependent apoptosis through death receptors and suppression of AKT in U937 human leukemic cells[J]. Phytother Res,2018,32(3):504-513.
[3]杜肖璇,孟蔚,严军,等.栽培技术对金银花品质及产量的影响[J].中国现代中药,2014,16(1):80-82.
[4]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技术出版社,2015:221.
[5] SHANG X,PAN H,LI M,et al. Lonicera japonica Thunb.:Ethnopharmacology,phytochemistry and pharmacology of an important traditional Chinese medicine[J]. J Ethnopharmacol,2011,138(1):1-21.
[6]宋亚玲,倪付勇,赵祎武.金银花化学成分研究进展[J].中草药,2014,45(24):3656-3662.
[7] KU S K,SEO B I,PARK J H,et al. Effect of Lonicerae Flos extracts on reflux esophagitis with antioxidant activity[J].World Journal of Gastroenterology:WJG,2009,15(38):4799-4822.
[8]胡璇,李卫东,李欧,等.滚筒杀青烘干加工方法对四倍体金银花药材质量的影响[J].中国中药杂志,2012,37(17):2554-2556.
[9]王永香,罗勇,沈娟,等.不同产地加工方法对江苏省东海县种植基地金银花质量影响的研究[J].中国中药杂志,2014,39(14):2665-2669.
[10]宋健,张会敏,石俊英.金银花最佳产地加工方法——杀青烘干干燥法[J].中药材,2008,31(4):489-491.
[11]武琳,黄菲,杨光.复方罗布麻颗粒中木犀草苷含量测定方法研究[J].中国中医药信息杂志,2015,22(4):91-93
[12]王川丕,刘新,诸力.两种方法测定茶叶游离氨基酸总量的差异及其原因分析[J].中国茶叶,2012,34(12):12-14.
[13]陈燕文,王玲娜,梁从莲,等.干燥方法对金银花多糖含量的影响[J].中国现代中药,2017,19(1):88-91.
[14] RAI Amit,KAMOCHI Hidetaka,SUZUKI Hideyuki,et al.De novo transcriptome assembly and characterization of nine tissues of Lonicera japonica to identify potential candidate genes involved in chlorogenic acid,luteolosides,and secoiridoid biosynthesis pathways[J]. J Nat Med,2017,71(1):1-15.
[15] CHAOWUTTIKUL C,PALANUVEJ C,RUANGRUNGSI N.Pharmacognostic Specification,Chlorogenic Acid Content,and In vitro Antioxidant Activities of Lonicera japonica Flowering Bud[J]. Pharmacognosy Res,2017,9(2):128-132.
[16]马静遥,刘新民,金哲雄,等.改善认知功能障碍中药研究进展[J].中国中医药信息杂志,2013,20(9):104-107.
[17] WU Jie,WANG Xiao-Chen,LIU Yang,et al. Flavone synthases from Lonicera japonica and L. macranthoides reveal differential flavone accumulation[J]. Sci Rep,2016,6(1):19245.
[18]谷民举,刘明岭,王琰.金银花质量与产地影响因素相关性研究[J].药学研究,2018,37(5):295-297.
[19]刘涛,王鸿,李青苗,等.不同采收期金银花的产地加工方法考察[J].中国实验方剂学杂志,2016,22(10):28-31.
[20]祁丹丹,戴伟东,谭俊峰,等.杀青方式对夏季绿茶化学成分及滋味品质的影响[J].茶叶科学,2016,36(1):18-26.
[21]崔文甲,曹世宁,王文亮,等.微波辅助提取金针菇、香菇菇柄中氨基酸的研究[J].食品工业,2018,39(9):96-99.