辽宁省鸡源沙门氏菌血清型与耐药性分析
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摘要
采集了辽宁省4个养殖场的鸡盲肠共632份样品,分离鉴定得到131株沙门氏菌,分离率为20.73%。同时对131株沙门氏菌进行了血清型鉴定和药物敏感性检测。结果显示,131株沙门氏菌共涵盖2个血清型,分别为肠炎沙门氏菌(S.Enteritidis,60.30%)和杜伊斯堡沙门氏菌(S.Duisburg,16.03%)。沙门氏菌对所检测的9类抗生素普遍耐药,只对头孢类抗生素较为敏感,但总体耐药率均较低,未超过70%耐药。
A total of 632 samples of chicken cecum were collected from 4 farms in Liaoning Province. After isolation, 131 strains of salmonella were obtained. The isolation rate was 20.73%.Meanwhile, serotype identification and drug sensitivity test were done on the 131 strains of salmonella. The results showed that the 131 strains of salmonella covered 2 serotypes, which were60.30% S. Enteritidis and 16.03% S. Duisburg. Salmonella was generally resistant to the 9 antibiotics tested. It was only sensitive to cephalosporin. However, the overall resistance rate was low, no more than 70%.
A total of 632 samples of chicken cecum were collected from 4 farms in Liaoning Province. After isolation, 131 strains of salmonella were obtained. The isolation rate was 20.73%.Meanwhile, serotype identification and drug sensitivity test were done on the 131 strains of salmonella. The results showed that the 131 strains of salmonella covered 2 serotypes, which were60.30% S. Enteritidis and 16.03% S. Duisburg. Salmonella was generally resistant to the 9 antibiotics tested. It was only sensitive to cephalosporin. However, the overall resistance rate was low, no more than 70%.
引文
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[2]施开创,李凤梅,邹联斌,等.鸡源致病性沙门氏菌的分离鉴定及血清型和药物敏感性分析[J].中国畜牧兽医,2015,42(8):2160-2168.
[3]王军,郑增忍,王晶钰.动物源性食品中沙门氏菌的风险评估[J].中国动物检疫,2007,24(4):23-25.
[4]刘艳红,李颖,吕淑霞.沙门氏菌和大肠杆菌耐药性的研究进展[J].黑龙江畜牧兽医,2016,(10上):88-90.
[5]张萍,冯芳.沙门氏菌的检测技术和方法的研究进展[J].食品安全质量检测学报,2015,6(5):1834-1841.
[6]李易.鸡源沙门氏菌的分离培养及鉴定[J].实验报告,2016,(6):166-167.
[7]李可,方莹,张晓峰,等.沙门氏菌的血清分型及分子鉴定研究进展[J].食品安全质量检测学报,2016,7(10):3947-3951.
[8]陈玲,张菊梅,杨小娟,等.沙门氏菌分型研究进展[J].微生物学通报,2016,43(3):648-654.
[9]朱奇,陆斌兴,覃有泉,等.沙门氏菌生物学研究进展[J].疾病监测与控制杂志,2015,9(7):474-478.
[10]梁德媚,许学斌,王伟芳,等.上海市肉鸽沙门氏菌的分离鉴定及流行血清型和耐药性分析[J].中国畜牧兽医,2016,43(12):3322-3328.
[11]李楠,赵思俊,王娟,等.肉鸡生产链中肠炎沙门氏菌耐药性分析及ERIC-PCR分型[J].食品科学,2016,37(3):120-124.
[12]吴秀娟,杨显超,李凯航,等.液相芯片技术在沙门氏菌血清型分型上的初步探索[J].上海畜牧兽医通讯,2015,(4):26-27.
[13] Hygiene, Management, Zoonoses Dept. Prevalence of Salmonella Species in Minced Beef and Meet Handlers and Their Drug Resistance[J]. Alexandria Journal of Veterinary Science, 2016, 49(1):122-128.
[14]侯雪娇,韦平.国内外动物源食品安全动态[J].广西畜牧兽医,2014,30(1):50-52.
[15] Mebrat Ejo, Legesse Garedew, Zabishwork Alebachew, et al. Prevalence and Antimicrobial Resistance of Salmonella Isolated from Animal-Origin Food Items in Gondar,Ethiopia[J].Bio Med Research International, 2016,(16):1-8.
[16]徐桂云,樊世杰.家禽沙门氏菌感染现状及不同国家的防治策略[J].中国家禽,2012,34(9):7-12.
[17]王嘉炜,肖英平,杨华,等.鸡肉源沙门氏菌血清型、药敏性及部分耐药基因研究[J].食品科学,2016,38(15):140-146.