北京市售鸽肉中大肠杆菌和沙门氏菌分离鉴定及耐药性分析
|
摘要
目的对北京市售鸽肉中大肠杆菌和沙门氏菌进行分离鉴定,并对分离菌株进行抗生素耐药性分析。方法使用缓冲蛋白胨水增菌液淋洗肉鸽样品后,使用大肠杆菌(E.coli,EC)增菌肉汤和丹麦国家血清研究院(Statens Serum Institute, SSI)肠道细菌琼脂分离大肠杆菌,使用四硫磺酸盐(tetrasulfonate, TT)增菌肉汤和木糖赖氨酸脱氧胆盐(xyloselysinedeoxycholate,XLD)琼脂平板分离沙门氏菌,使用生化鉴定进行可疑菌确认。参照CLSI2016版推荐的肉汤稀释法,测定15种抗生素对所分离大肠杆菌和沙门氏菌的最低抑菌浓度(minimalinhibitory concentrations,MICs)。结果样品(n=200)中大肠杆菌(n=104)检出率为52.0%,沙门氏菌(n=41)检出率为21.0%。所分离的42株沙门氏菌菌株均对头孢他啶、氨曲南、厄他培南和替加环素敏感,对氨苄西林、萘啶酸、环丙沙星、四环素、复方新诺明和氯霉素的耐药率在30%以上,部分菌株对头孢噻肟(4.8%)和黏菌素(2.4%)耐药。所分离的104株大肠杆菌菌株均对替加环素敏感,对氨苄西林、头孢噻肟、氨曲南、萘啶酸、环丙沙星、四环素、复方新诺明、氯霉素和卡那霉素的耐药率在30%以上,部分菌株对头孢他啶(2.9%)、厄他培南和(1.0%)和黏菌素(9.6%)耐药。结论北京市售鸽肉是耐药大肠杆菌和沙门菌的重要储存库,相较于我国其他地区市售猪肉,鸡肉,牛肉等分离的耐药菌株,表现出不同的耐药谱,耐药率相对较低,但是北京市售鸽肉所携带的菌株已经累积了复杂的耐药特征,有必要对其中存在的耐药机制进行系统研究。
Objective To isolate and identify the Escherichia coli and Salmonella in the pigeon meat sold in Beijing,and analyze the antibiotic resistance of isolates.Methods After washing the samples of the pigeons with the buffer protein hydrolysate,the E.coli(EC) enrichment broth and the Danish National Institute of Serum Institute(SSI) enteric bacteria agar were used to isolate E.coli.Salmonella was isolated from tetrasulfonate(TT) enrichment broth and xylose lysine deoxycholate(XLD) agar plates,and biochemical identification was used for confirmation of suspicious bacteria.The minimum inhibitory concentrations(MICs) of 15 antibiotics against E.coli and Salmonella isolated were determined according to the broth dilution method recommended by CLSI 2016.Results The detection rate of E.coli(n=104) in the sample(n=200) was 52.0%,and the detection rate of Salmonella(n=41) was 21.0%.The 42 strains of Salmonella isolated were sensitive to ceftazidime,aztreonam,ertapenem and tigecycline,resistance to ampicillin,nalidixic acid,ciprofloxacin,tetracycline,co-trimoxazole and chloramphenicol over 30%,and some strains were resistant to cefotaxime(4.8%) and colistin(2.4%).The 104 strains of E.coli isolated were all sensitive to tigecycline,resistance to ampicillin,cefotaxime,aztreonam,nalidixic acid,ciprofloxacin,tetracycline,cotrimoxazole,chloramphenicol and kanamycin over 30%,and some strains were resistant to ceftazidime(2.9%),ertapenem and(1.0%) and colistin(9.6%).Conclusion Beijing pigeon meat is an important repository for drug-resistant E.coli and Salmonella.Compared with other commercially available pork,chicken,beef and other resistant strains in other regions of China,they show different drug resistance profiles and lower resistance rates.However,the strains carried by pigeons sold in Beijing have accumulated complex drug resistance characteristics,and it is necessary to systematically study the resistance mechanisms existing therein.
Objective To isolate and identify the Escherichia coli and Salmonella in the pigeon meat sold in Beijing,and analyze the antibiotic resistance of isolates.Methods After washing the samples of the pigeons with the buffer protein hydrolysate,the E.coli(EC) enrichment broth and the Danish National Institute of Serum Institute(SSI) enteric bacteria agar were used to isolate E.coli.Salmonella was isolated from tetrasulfonate(TT) enrichment broth and xylose lysine deoxycholate(XLD) agar plates,and biochemical identification was used for confirmation of suspicious bacteria.The minimum inhibitory concentrations(MICs) of 15 antibiotics against E.coli and Salmonella isolated were determined according to the broth dilution method recommended by CLSI 2016.Results The detection rate of E.coli(n=104) in the sample(n=200) was 52.0%,and the detection rate of Salmonella(n=41) was 21.0%.The 42 strains of Salmonella isolated were sensitive to ceftazidime,aztreonam,ertapenem and tigecycline,resistance to ampicillin,nalidixic acid,ciprofloxacin,tetracycline,co-trimoxazole and chloramphenicol over 30%,and some strains were resistant to cefotaxime(4.8%) and colistin(2.4%).The 104 strains of E.coli isolated were all sensitive to tigecycline,resistance to ampicillin,cefotaxime,aztreonam,nalidixic acid,ciprofloxacin,tetracycline,cotrimoxazole,chloramphenicol and kanamycin over 30%,and some strains were resistant to ceftazidime(2.9%),ertapenem and(1.0%) and colistin(9.6%).Conclusion Beijing pigeon meat is an important repository for drug-resistant E.coli and Salmonella.Compared with other commercially available pork,chicken,beef and other resistant strains in other regions of China,they show different drug resistance profiles and lower resistance rates.However,the strains carried by pigeons sold in Beijing have accumulated complex drug resistance characteristics,and it is necessary to systematically study the resistance mechanisms existing therein.
引文
[1]赵宝华,卜柱,徐步,等.肉鸽大肠杆菌的分离与鉴定[J].经济动物学报,2010,14(4):225-227.Zhao BH,Bu Z,Xu B,et al.Isolation and identification of Escherichia coli from pigeons[J].J Econ Anim,2010,14(4):225-227.
[2]杨连楷.鸽病防治技术[M].北京:金盾出版社,2007.Yang LK.Pigeon disease control technology[M].Beijing:Golden Shield Press,2007.
[3]柏文芳,伍清林,金兰梅,等.肉鸽大肠杆菌的分离鉴定与耐药性的研究[J].家畜生态学报,2013,34(3):58-61.Bo WF,Wu QL,Jin LM,et al.Isolation,identification and drug resistance of Escherichia coli from meat pigeons[J].Acta Ecolog Anim Domast,2013,34(3):58-61.
[4]周佰祥.肉鸽致病性大肠杆菌的分离鉴定及药敏试验[J].畜牧与饲料科学,2011,(11):134-135.Zhou BX.Isolation,identification and drug sensitivity test of pathogenic E.coli in meat pigeon[J].Anim Husb Feed Sci,2011,(11):134-135.
[5]刘玥,顾其芳,刘诚,等.2016年上海市市售肉制品中沙门菌耐药谱与分型研究[J].中国食品卫生杂志,2018,(2):132-138.Liu Y,Gu QF,Liu C,et al.Serotyping,molecular subtyping and drug resistance patterns of Salmonella isolates from retail meat in Shanghai,2016[J].Chin J Food Hyg,2018,(2):132-138.
[6]梁德媚,许学斌,王伟芳,等.上海市肉鸽沙门氏菌的分离鉴定及流行血清型和耐药性分析[J].中国畜牧兽医,2016,43(12):3322-3328.Liang DM,Xu XB,Wang WW,et al.Isolation,identification and analysis of serotype and drug resistance Salmonella from pigeon in Shanghai[J].China Anim Husband Veter Med,2016,43(12):3322-3328.
[7]Vlahovic K,Matica B,Bata I,et al.Campylobacter,Slamonella and Chlamydia in freeliving birds of Croatia[J].Wildlife Res,2004,50(3):127-132.
[8]Russo ET,Biggerstaff G,Hoekstra RMA,et al.Arecurrent,multistate outbreak of Salmonella serotype agona infections associated with dry,unsweetened cereal consumption,United States,2008[J].Food Protect,2013,76(2):227-230.
[9]Zhang J,Jin H,Hu J,et al.Serovars and antimicrobial resistance of nontyphoid Salmonella from human patients in Shanghai,China,2006-2010[J].Epidem Infect,2014,142(4):826-832.
[10]Dai K,Zhang J,Yang XW,et al.Antimicrobial susceptibility and molecular typing of Salmonella agona isolated from humans and other sources[J].Foodborn Pathog Diseas,2014,(11):1-6.
[11]吴云凤,袁宝君,乔昕,等.肉鸡胴体中沙门氏菌的分离鉴定及多重耐药谱研究[J].南京医科大学学报(自然科学版),2012,(1):125-128.Wu YF,Yuan BJ,Qiao X,et al.Separation and identification of multiple-antimicrobial-resistant of Salmonella on chickencarcass[J].Acta Univ Med Nanjing(Nat Sci Ed),2012,(1):125-128.
[12]赖婧,刘洋,汪宇,等.800株不同动物源大肠杆菌的耐药性监测[J].中国兽医杂志,2011,47(4):12-14.Lai J,Liu Y,Wang Y,et al.Surveillance of antimicrobial resistance among Escherichia coli isolates from different animals in Sichuan province in2007[J].Chin J Veter Med,2011,47(4):12-14.
[13]Yeliz Y,Zafer G,Sebnem P,et al.Incidence and antibiotic resistance of Salmonella spp.On raw chicken carcasses[J].Food Res Inter,2011,44(3):725-728.
[14]FDA BAM.Enumeration of Escherichia coli and the Coliform bacteria[Z].
[15]GB 4789.36-2016食品安全国家标准食品微生物学检验大肠埃希氏菌O157:H7/NM检验[S].GB 4789.36-2016 National food safety standards-Food microbiological analysis-Detection of Escherichia coli O157:H7/NM[S]
[16]FDA BAM.Salmonella[Z].
[17]GB4789.4-2016食品安全国家标准食品微生物学检验沙门氏菌检验[S].GB 4789.4-2016 National food safety standards-Food microbiological analysis-Detection of Salmonella[S]
[18]Performance Standard for Antimicrobial Susceptibility Testing:Clinical and Laboratory Standards Institute M100-S26[Z].
[2]杨连楷.鸽病防治技术[M].北京:金盾出版社,2007.Yang LK.Pigeon disease control technology[M].Beijing:Golden Shield Press,2007.
[3]柏文芳,伍清林,金兰梅,等.肉鸽大肠杆菌的分离鉴定与耐药性的研究[J].家畜生态学报,2013,34(3):58-61.Bo WF,Wu QL,Jin LM,et al.Isolation,identification and drug resistance of Escherichia coli from meat pigeons[J].Acta Ecolog Anim Domast,2013,34(3):58-61.
[4]周佰祥.肉鸽致病性大肠杆菌的分离鉴定及药敏试验[J].畜牧与饲料科学,2011,(11):134-135.Zhou BX.Isolation,identification and drug sensitivity test of pathogenic E.coli in meat pigeon[J].Anim Husb Feed Sci,2011,(11):134-135.
[5]刘玥,顾其芳,刘诚,等.2016年上海市市售肉制品中沙门菌耐药谱与分型研究[J].中国食品卫生杂志,2018,(2):132-138.Liu Y,Gu QF,Liu C,et al.Serotyping,molecular subtyping and drug resistance patterns of Salmonella isolates from retail meat in Shanghai,2016[J].Chin J Food Hyg,2018,(2):132-138.
[6]梁德媚,许学斌,王伟芳,等.上海市肉鸽沙门氏菌的分离鉴定及流行血清型和耐药性分析[J].中国畜牧兽医,2016,43(12):3322-3328.Liang DM,Xu XB,Wang WW,et al.Isolation,identification and analysis of serotype and drug resistance Salmonella from pigeon in Shanghai[J].China Anim Husband Veter Med,2016,43(12):3322-3328.
[7]Vlahovic K,Matica B,Bata I,et al.Campylobacter,Slamonella and Chlamydia in freeliving birds of Croatia[J].Wildlife Res,2004,50(3):127-132.
[8]Russo ET,Biggerstaff G,Hoekstra RMA,et al.Arecurrent,multistate outbreak of Salmonella serotype agona infections associated with dry,unsweetened cereal consumption,United States,2008[J].Food Protect,2013,76(2):227-230.
[9]Zhang J,Jin H,Hu J,et al.Serovars and antimicrobial resistance of nontyphoid Salmonella from human patients in Shanghai,China,2006-2010[J].Epidem Infect,2014,142(4):826-832.
[10]Dai K,Zhang J,Yang XW,et al.Antimicrobial susceptibility and molecular typing of Salmonella agona isolated from humans and other sources[J].Foodborn Pathog Diseas,2014,(11):1-6.
[11]吴云凤,袁宝君,乔昕,等.肉鸡胴体中沙门氏菌的分离鉴定及多重耐药谱研究[J].南京医科大学学报(自然科学版),2012,(1):125-128.Wu YF,Yuan BJ,Qiao X,et al.Separation and identification of multiple-antimicrobial-resistant of Salmonella on chickencarcass[J].Acta Univ Med Nanjing(Nat Sci Ed),2012,(1):125-128.
[12]赖婧,刘洋,汪宇,等.800株不同动物源大肠杆菌的耐药性监测[J].中国兽医杂志,2011,47(4):12-14.Lai J,Liu Y,Wang Y,et al.Surveillance of antimicrobial resistance among Escherichia coli isolates from different animals in Sichuan province in2007[J].Chin J Veter Med,2011,47(4):12-14.
[13]Yeliz Y,Zafer G,Sebnem P,et al.Incidence and antibiotic resistance of Salmonella spp.On raw chicken carcasses[J].Food Res Inter,2011,44(3):725-728.
[14]FDA BAM.Enumeration of Escherichia coli and the Coliform bacteria[Z].
[15]GB 4789.36-2016食品安全国家标准食品微生物学检验大肠埃希氏菌O157:H7/NM检验[S].GB 4789.36-2016 National food safety standards-Food microbiological analysis-Detection of Escherichia coli O157:H7/NM[S]
[16]FDA BAM.Salmonella[Z].
[17]GB4789.4-2016食品安全国家标准食品微生物学检验沙门氏菌检验[S].GB 4789.4-2016 National food safety standards-Food microbiological analysis-Detection of Salmonella[S]
[18]Performance Standard for Antimicrobial Susceptibility Testing:Clinical and Laboratory Standards Institute M100-S26[Z].