miRNA单核苷酸多态性与宫颈癌的相关性研究
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摘要
目的探讨miR-27a、miR-34a/c和miR-107单核苷酸多态性与宫颈癌的相关性。方法收集104例宫颈癌及186例对照,采用多重PCR技术对miR-27a,miR-34a/c和miR-107单核苷酸多态性进行分析,卡方检验分析各基因型与宫颈癌之间的相关性。结果在病例组和对照组中,miR-27a rs895819 TT、CT和CC基因型的分布为52.9%、44.1%、2.9%和59.8%、33.7%、6.5%,miR-34b/c rs4938723 TT、CT和CC基因型的分布为51.9%、35.6%、12.5%和47.3%、44.1%、8.6%,miR-107 rs2296616 TT、CT和CC基因型的分布为80.8%、19.2%、0%和81.2%、17.7%、1.1%,各位点基因型频数分布差别均无统计学意义(P>0.05)。进一步分层分析发现,不同年龄的各基因型分布在对照组和病例组中差异也均无统计学意义(P>0.05)。结论 miR-27a rs895819、miR-34b/c rs4938723和miR-107 rs2296616基因多态性可能与宫颈癌发生无关。
Objective To investigate the relationship between gene polymorphism of miR-27 a,miR-34a/c and miR-107 and cervical cancer risk. Methods A case-control study was carried out including 104 patients with cervical cancer and 186 controls. The gene polymorphism of miR-27 a,miR-34a/c and miR-107 in patients and controls were detected by multiple PCR method. The association between the genotypes and cervical cancer was analyzed by Chi-square test. Results The genotypes of miR-27 a rs895819 were 52. 9% for TT,44. 1% for CT,2. 9% for CC in cases and 59. 8% for TT,33. 7% for CT,6. 5% for CC in controls. The genotypes of miR-34b/c rs4938723 were 51. 9% for TT,35. 6% for CT,12. 5% for CC in cases and 47. 3% for TT,44. 1% for CT,8. 6% for CC in controls. The genotypes of miR-107 rs2296616 were 80. 8% for TT,19. 2% for CT,0% for CC in cases and 81. 2% for TT,17. 7% for CT,1. 1% for CC in controls. The genotype distribution of miR-27 a rs895819,miR-34b/c rs4938723 and miR-107 rs2296616 polymorphism in the cases and the controls had no significantly statistical difference(P > 0. 05). Further age-stratified analysis showed that these three polymorphism genotype frequencies distribution had no statistical significance in the cases and the controls(P > 0. 05). Conclusion There is no association between gene polymorphism of miR-27 a rs895819,miR-34b/c rs4938723 and miR-107 rs2296616 and cervical cancer.
Objective To investigate the relationship between gene polymorphism of miR-27 a,miR-34a/c and miR-107 and cervical cancer risk. Methods A case-control study was carried out including 104 patients with cervical cancer and 186 controls. The gene polymorphism of miR-27 a,miR-34a/c and miR-107 in patients and controls were detected by multiple PCR method. The association between the genotypes and cervical cancer was analyzed by Chi-square test. Results The genotypes of miR-27 a rs895819 were 52. 9% for TT,44. 1% for CT,2. 9% for CC in cases and 59. 8% for TT,33. 7% for CT,6. 5% for CC in controls. The genotypes of miR-34b/c rs4938723 were 51. 9% for TT,35. 6% for CT,12. 5% for CC in cases and 47. 3% for TT,44. 1% for CT,8. 6% for CC in controls. The genotypes of miR-107 rs2296616 were 80. 8% for TT,19. 2% for CT,0% for CC in cases and 81. 2% for TT,17. 7% for CT,1. 1% for CC in controls. The genotype distribution of miR-27 a rs895819,miR-34b/c rs4938723 and miR-107 rs2296616 polymorphism in the cases and the controls had no significantly statistical difference(P > 0. 05). Further age-stratified analysis showed that these three polymorphism genotype frequencies distribution had no statistical significance in the cases and the controls(P > 0. 05). Conclusion There is no association between gene polymorphism of miR-27 a rs895819,miR-34b/c rs4938723 and miR-107 rs2296616 and cervical cancer.
引文
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[15]Xiong XD,Luo XP,Cheng J,et al.A genetic variant in pre-mi R-27a is associated with a reduced cervical cancer risk in southern Chinese women[J].Gynecologic Oncology,2014,132(2):450-454.
[16]Gao LB,Li LJ,Pan XM,et al.A genetic variant in the promoter region of mi R-34b/c is associated with a reduced risk of colorectal cancer[J].Biological Chemistry,2013,394(3):415-420.
[17]刘莉,陈小军,徐妍,等.mi R-34b/c启动子区多态性与原发性肝细胞肝癌易感性的关联研究[J].中华疾病控制杂志,2011,15(7):551-554.
[18]Yuan F,Sun R,Chen P,et al.Combined analysis of pri-mi R-34b/c rs4938723 and TP53 Arg72Pro with cervical cancer risk[J].Tumour biology:the journal of the International Society for Oncodevelopmental Biology and Medicine,2016,37(5):6267-6273.
[19]Huang YS,Dai Y,Yu XF,et al.Microarray analysis of micro RNA expression in hepatocellular carcinoma and non-tumorous tissues without viral hepatitis[J].Journal of Gastroenterology and Hepatology,2008,23(1):87-94.
[20]Wong TS,Liu XB,Wong BY,et al.Mature mi R-184 as potential oncogenic micro RNA of squamous cell carcinoma of tongue[J].Clinical cancer research:an official journal of the American Association for Cancer Research,2008,14(9):2588-2592.
[21]Guo Y,Chen Z,Zhang L,et al.Distinctive micro RNA profiles relating to patient survival in esophageal squamous cell carcinoma[J].Cancer Research,2008,68(1):26-33.
[2]Khvostova EP,Pustylnyak VO,Gulyaeva LF.Genetic polymorphism of estrogen metabolizing enzymes in Siberian women with breast cancer[J].Genetic Testing and Molecular Biomarkers,2012,16(3):167-173.
[3]Wojcicka A,De La Chapelle A,Jazdzewski K.Micro RNA-related sequence variations in human cancers[J].Human Genetics,2014,133(4):463-469.
[4]Wang YL,Gong WG,Yuan QL.Effects of mi R-27a upregulation on thyroid cancer cells migration,invasion,and angiogenesis[J].Genetics and molecular research:GMR,2016,15(4):gmr15049070.
[5]Hiyoshi Y,Schetter AJ,Okayama H,et al.Increased micro RNA-34b and-34c predominantly expressed in stromal tissues is associated with poor prognosis in human colon cancer[J].PLOS One,2015,10(4):e0124899.
[6]Wang S,Ma G,Zhu H,et al.mi R-107 regulates tumor progression by targeting NF1 in gastric cancer[J].Scientific Reports,2016,6:36531.
[7]Jiang Y,Lin DH,Xu JP,et al.Genotype GG of rs895819 functional polymorphism within mi R-27a might increase genetic susceptibility to colorectal cancer in Han Chinese population[J].Journal of Clinical Laboratory Analysis,2016,30(4):351-355.
[8]Ji TX,Zhi C,Guo XG,et al.Mi R-34b/c rs4938723 polymorphism significantly decreases the risk of digestive tract cancer:metaanalysis[J].Asian Pacific journal of cancer prevention:APJCP,2015,16(14):6099-6104.
[9]Martello G,Rosato A,Ferrari F,et al.A Micro RNA targeting dicer for metastasis control[J].Cell,2010,141(7):1195-1207.
[10]Wang S,Lv C,Jin H,et al.A common genetic variation in the promoter of mi R-107 is associated with gastric adenocarcinoma susceptibility and survival[J].Mutation research,2014,769:35-41.
[11]Liu F,Dear K,Huang L,et al.Association between micro RNA-27a rs895819 polymorphism and risk of colorectal cancer:A metaanalysis[J].Cancer Genetics,2016,209(9):388-394.
[12]Hashemi M,Danesh H,Bizhani F,et al.Pri-mi R-34b/c rs4938723 polymorphism increased the risk of prostate cancer[J].Cancer biomarkers:section A of Disease markers,2017,18(2):155-159.
[13]Zhong S,Chen Z,Xu J,et al.Pre-mir-27a rs895819polymorphism and cancer risk:a meta-analysis[J].Molecular Biology Reports,2013,40(4):3181-3186.
[14]张博,陈洁平.micro RNA相关基因rs895819、rs6505162和rs2292832的SNP变异与B细胞淋巴瘤患者生存关系的研究[J].重庆医学,2013,42(16):1801-1803.
[15]Xiong XD,Luo XP,Cheng J,et al.A genetic variant in pre-mi R-27a is associated with a reduced cervical cancer risk in southern Chinese women[J].Gynecologic Oncology,2014,132(2):450-454.
[16]Gao LB,Li LJ,Pan XM,et al.A genetic variant in the promoter region of mi R-34b/c is associated with a reduced risk of colorectal cancer[J].Biological Chemistry,2013,394(3):415-420.
[17]刘莉,陈小军,徐妍,等.mi R-34b/c启动子区多态性与原发性肝细胞肝癌易感性的关联研究[J].中华疾病控制杂志,2011,15(7):551-554.
[18]Yuan F,Sun R,Chen P,et al.Combined analysis of pri-mi R-34b/c rs4938723 and TP53 Arg72Pro with cervical cancer risk[J].Tumour biology:the journal of the International Society for Oncodevelopmental Biology and Medicine,2016,37(5):6267-6273.
[19]Huang YS,Dai Y,Yu XF,et al.Microarray analysis of micro RNA expression in hepatocellular carcinoma and non-tumorous tissues without viral hepatitis[J].Journal of Gastroenterology and Hepatology,2008,23(1):87-94.
[20]Wong TS,Liu XB,Wong BY,et al.Mature mi R-184 as potential oncogenic micro RNA of squamous cell carcinoma of tongue[J].Clinical cancer research:an official journal of the American Association for Cancer Research,2008,14(9):2588-2592.
[21]Guo Y,Chen Z,Zhang L,et al.Distinctive micro RNA profiles relating to patient survival in esophageal squamous cell carcinoma[J].Cancer Research,2008,68(1):26-33.