油茶F1代苗期叶表型性状遗传多样性研究
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摘要
探索油茶(Camellia oleifera)杂交F1代叶表型性状的遗传变异特征,为杂交后代早期选择提供依据,以"岑软24号×湘林210"杂交F1代的62个单株为试验材料,对其叶表型性状多样性进行研究。结果表明:1)14个性状的变异系数为2.21%～37.50%,其中叶面积的变异系数最小,仅为2.21%,而叶片鲜重、干重和饱和鲜重的变异系数均较大,分别为35.42%、37.50%和36.84%;2)各性状均具有较好的连续性正态分布趋势;3)14个性状在F1代的中亲优势率为-16.60%～44.66%,超亲优势率为-21.05%～38.06%,其中10个性状的中亲优势值为正值,且遗传传递力都超过100%,叶面积、叶周长、叶长和叶片干物质含量这4个性状的超亲优势值为正值;4)91对相关性分析中有65对达到极显著水平,8对达到显著水平;5)系统聚类将F1代的62个单株分为2个类群、4个亚类,聚类结果充分反映了各类群的特征。
In order to analyze the characteristics of genetic variation of leaf phenotypic traits of Camellia oleifera hybridized F1 generation and provide basis for early selection of hybrid offspring,the diversity of leaf phenotypic traits in 62 individual plants of hybridized F1 generation of"Cen soft 24,Xiang Lin 210"was studied.The results showed that the variation coefficients of 14 traits ranged from 2.21%to 37.50%,and the minimum one was the leaf area,only 2.21%;while the variation coefficients of fresh weight,dry weight and saturated fresh weight of the leaves were 35.42%,37.50% and 36.84%,respectively.All the traits'variation trend preferably presented continuous normal distribution.The mid-parent heterosis ratio of the F1generation's 14 traits were from-16.60%to 44.66%,and the suppor-parent heterosis ratio of those ranged from~(-2)1.05% to 38.06%.Among them,10traits'mid-parent heterosis ratio was positive,and whose inheritance of transfer ability exceeded 100%;4traits of leaf area,circumference,length and dry matter content,whose suppor-parent heterosis ratio were positive values.In all the 91 pairs,65pairs of them were in highly significant correlation with each other,and 8pairs were in significant correlation.Sixty two individual plants of F1 generation were divided into 2groups and 4subclasses through system clustering,that fully reflected the characteristics of each group.
In order to analyze the characteristics of genetic variation of leaf phenotypic traits of Camellia oleifera hybridized F1 generation and provide basis for early selection of hybrid offspring,the diversity of leaf phenotypic traits in 62 individual plants of hybridized F1 generation of"Cen soft 24,Xiang Lin 210"was studied.The results showed that the variation coefficients of 14 traits ranged from 2.21%to 37.50%,and the minimum one was the leaf area,only 2.21%;while the variation coefficients of fresh weight,dry weight and saturated fresh weight of the leaves were 35.42%,37.50% and 36.84%,respectively.All the traits'variation trend preferably presented continuous normal distribution.The mid-parent heterosis ratio of the F1generation's 14 traits were from-16.60%to 44.66%,and the suppor-parent heterosis ratio of those ranged from~(-2)1.05% to 38.06%.Among them,10traits'mid-parent heterosis ratio was positive,and whose inheritance of transfer ability exceeded 100%;4traits of leaf area,circumference,length and dry matter content,whose suppor-parent heterosis ratio were positive values.In all the 91 pairs,65pairs of them were in highly significant correlation with each other,and 8pairs were in significant correlation.Sixty two individual plants of F1 generation were divided into 2groups and 4subclasses through system clustering,that fully reflected the characteristics of each group.
引文
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[15]李海波,丁红梅,陈友吾,等.12个油茶品种的SSR特征指纹鉴别[J].中国粮油学报,2017,32(10):171-178.
[16]梁晓静,李开祥,梁文汇,等.不同品种肉桂叶表型性状分析[J].广西林业科学,2016,45(1):40-44.
[2]朱振家,姜成英,史艳虎,等.库源比改变对油橄榄产量及源叶光合作用的调节[J].中国农业科学,2015,48(3):546-554.ZHU Z J,JIANG C Y,SHI Y H,et al.Response of yield and leaf photosynthesis to sink-source ratio altering demand in olice[J].Scientia Agricultura Scinica,2015,48(3):546-554.(in Chinese)
[3]刁松锋,李芳东,段伟,等.柿杂交F1代叶表型遗传多样性研究[J].中国农业大学学报,2017,22(2):32-44.DIAO S F,LI F D,DUAN W,et al.Genetic diversity phenotypic traits of leaves in F1progeny of persimmon[J].Journal of China Agricultural University,2017,22(2):32-44.(in Chinese)
[4]张晶,赵成章,李雪萍,等.嘉峪关草湖湿地芦苇净光合速率与叶面积和叶厚度的关系[J].生态学报,2018,38(17):1-8.ZHANG J,ZHAO C,LI X,et al.The relationship between the net photosynthetic rate and leaf area and thickness of Phragmites australis in the grass lake wetlands of Jiayuguan[J].Acta Ecoligica Sinica,2018,38(17):1-8.(in Chinese)
[5]丁曼,温仲明,郑颖.黄土丘陵区植物功能行写的尺度变化与依赖[J].生态学报,2014,34(9):2308-2315.
[6]段媛媛,宋丽娟,牛素旗,等.不同林龄刺槐叶功能性状差异及其土壤养分的关系[J].应用生态学报,2017,28(1):28-36.DUAN Y Y,SONG L J,NIU S Q,et al.Variation in leaf functional traits of different-aged Robinia pseudoacacia communities and relationships with soil nutrients[J].Chinese Journal of Applied Ecology,2017,28(1):28-36.(in Chinese)
[7]罗璐,申国珍,谢宗强,等.神农架海拔梯度上4种典型森林的乔木叶片功能性状特征[J].生态学报,2011,31(21):6420-6428.
[8]王鸣枭,刘政礼,文翠萍,等.柱型苹果杂交后代不同株型枝叶及光合参数比较[J].青岛农业大学学报(自然科学版),2017,34(2):95-99.WANG M X,LIU Z L,WEN C P,et al.Comparision of branch leaf and photosynthetic parameters in different plant types of columnar apple’s hybrid progeny[J].Journal of Qingdao Agricultural University:Natural Science,2017,34(2):95-99.(in Chinese)
[9]曾祥艳,陈金艳,廖健明,等.广西多穗柯叶片性状变异及幼苗生长量研究[J].广西植物,2015,35(6):885-890.ZENG X Y,CHEN J Y,LIAO J M,et al.Variation of leaf traits and seedling growth of Lithocarpus polystachyus in Guangxi[J].Guihaia,2015,35(6):885-890.(in Chinese)
[10]赵爽,任俊杰,石鹤飞,等.早实核桃叶片性状遗传规律研究[J].植物遗传资源学报,2015,16(3):659-663.ZHAO S,REN J J,SHI H F,et al.Study on leaf traits genetic law of precocious walnut[J].Journal of Plant Genetic Resources,2015,16(3):659-663.(in Chinese)
[11]张小猜,赵政阳,樊红科,等.苹果杂种F1代叶片性状分离及早期选择研究[J].西北农业学报,2009,18(5):228-331.ZHANG X C,ZHAO Z Y,FAN H K,et al.Study on the leaf trait segregation of apple hybrid seedlings and application in pre-selection[J].Acta Agriculturae Boreli-occidentalis Sinica,2009,18(5):228-331.(in Chinese)
[12]魏永成,李周岐,李煜,等.杜仲杂交子代苗期表型性状的遗传分析[J].西北农林科技大学学报:自然科学版,2012,40(8):137-143.WEI Y C,LI Z Q,LI Y,et al.Genetic analysis of morphological traits of Eucommia ulmoides oliver’s hybrid offspring[J].Journal of Northwest A&F University:Nat.Sci.Ed.,2012,40(8):137-143.(in Chinese)
[13]李春明,严冬,夏辉,等.毛白杨种内杂交无性系苗期生长量及叶片性状变异研究[J].植物研究,2016,36(1):62-67.LI C M,YAN D,XIA H,et al.Variations of growth and leaf traits of intraspecific hybridization clones of Populus tomentosa[J].Bulletin of Botanical Research,2016,36(1):62-67.(in Chinese)
[14]王雷存,万怡震,高华,等.‘富士×嘎拉’F1代杂种苗早期选择试验[J].西北林学院学报,2010,25(2):80-82.WANG L C,WAN Y Z,GAO H,et al.Preliminary survey on early selection in the seedlings of the apple ‘Fuji×Gala’crosses[J].Journal of Northwest Forestry University,2010,25(2):80-82.(in Chinese)
[15]李海波,丁红梅,陈友吾,等.12个油茶品种的SSR特征指纹鉴别[J].中国粮油学报,2017,32(10):171-178.
[16]梁晓静,李开祥,梁文汇,等.不同品种肉桂叶表型性状分析[J].广西林业科学,2016,45(1):40-44.