高效液相色谱法测定防晒霜中二乙基己基丁酰胺基三嗪酮与维生素E的含量
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摘要
目的应用高效液相色谱测定防晒霜中二乙基己基丁酰胺基三嗪酮与维生素E的含量。方法采用Alltima C_(18)色谱柱(150 mm×4.6 mm,5μm),甲醇为流动相,梯度流速洗脱,检测波长为210nm,进样量为20μL。结果制剂辅料不干扰二乙基己基丁酰胺基三嗪酮与维生素E的测定,二乙基己基丁酰胺基三嗪酮在24.5~391.1μg·mL~(-1)与峰面积线性关系良好(r=0.9991),维生素E在24.5~392.0μg·mL~(-1)与峰面积线性关系良好(r=0.9999)。两者精密度和稳定性试验RSD均小于1.0%,重复性与回收试验RSD均小于3.0%。结论该方法准确,简便,灵敏度高,专属性强,可同时测定防晒霜中二乙基己基丁酰胺基三嗪酮与维生素E的含量。
Objective To establish an HPLC method for the simultaneous determination of iscotrizinol and vitamin E in sun lotions. Methods An Alltima C18 column(150 mm×4.6 mm, 5 μm) was used for the separation with mobile phase of methanol and velocity gradient elution. The detection wavelength was 210 nm. The injection volumn was 20 μL. Results The excipients did not interfere the analysis of iscotrizinol and vitamin E. The linear range of iscotrizinol was 24.5-391.1 μg·mL~(-1)( r = 0.9991). The linear range of vitamin E was 24.5-392.0 μg·mL~(-1)( r = 0.9999). The precision and stability of the two components RSDs were below 1.0%. RSDs of repetitive tests and the spiked recoveries were below 3.0%. Conclusion The method is accurate and simple with high sensitivity and specificity, and which is suitable for the simultaneous determination of iscotrizinol and vitamin E in sun lotions.
Objective To establish an HPLC method for the simultaneous determination of iscotrizinol and vitamin E in sun lotions. Methods An Alltima C18 column(150 mm×4.6 mm, 5 μm) was used for the separation with mobile phase of methanol and velocity gradient elution. The detection wavelength was 210 nm. The injection volumn was 20 μL. Results The excipients did not interfere the analysis of iscotrizinol and vitamin E. The linear range of iscotrizinol was 24.5-391.1 μg·mL~(-1)( r = 0.9991). The linear range of vitamin E was 24.5-392.0 μg·mL~(-1)( r = 0.9999). The precision and stability of the two components RSDs were below 1.0%. RSDs of repetitive tests and the spiked recoveries were below 3.0%. Conclusion The method is accurate and simple with high sensitivity and specificity, and which is suitable for the simultaneous determination of iscotrizinol and vitamin E in sun lotions.
引文
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[3]戚燕,王志鹏,吴松.防晒类化妆品中二乙基己基丁酰胺基三嗪酮的高效液目色谱测定法[J].环境与健康杂志,2013,30(9):818-820.
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[5]覃芳敏,刘露,朱小娜,等.高效液相色谱法同时测定防晒类化妆品中二苯酮-2等3种防晒剂组分的含量[J].广州化学,2017,42(4):54-58.
[6]陆松伟,虞慧华,陈方剑,等.用双波长紫外分光光度法测定尿囊素维E乳膏中维生素E的含量[J].药学服务与研究,2015,15(5):354-356.
[7]陈方剑,陆松伟,宋洪杰,等.用HPLC法测定珍珠维E乳膏中维生素B6和维生素E的含量[J].药学服务与研究,2015,15(3):201-204.
[8]傅若秋,孟德胜,卢来春,等.高效液相色谱法测定薇依霜中维生素E和羟苯乙酯含量[J].中国药业,2012,21(7):12-13.
[9]徐文峰,徐硕,何笑荣,等.天然维生素E软胶囊中维生素E的含量测定[J].中国医药导报,2017,14(30):136-138,167.
[2]罗绍强,唐青涛,吴志韵.美白日霜中防晒成分的检测[J].日用化学品科学,2010,33(8):14-17.
[3]戚燕,王志鹏,吴松.防晒类化妆品中二乙基己基丁酰胺基三嗪酮的高效液目色谱测定法[J].环境与健康杂志,2013,30(9):818-820.
[4]许艳萍,沙宪政.维生素E功能浅析[J].中国医学工程,2014,22(3):186-187.
[5]覃芳敏,刘露,朱小娜,等.高效液相色谱法同时测定防晒类化妆品中二苯酮-2等3种防晒剂组分的含量[J].广州化学,2017,42(4):54-58.
[6]陆松伟,虞慧华,陈方剑,等.用双波长紫外分光光度法测定尿囊素维E乳膏中维生素E的含量[J].药学服务与研究,2015,15(5):354-356.
[7]陈方剑,陆松伟,宋洪杰,等.用HPLC法测定珍珠维E乳膏中维生素B6和维生素E的含量[J].药学服务与研究,2015,15(3):201-204.
[8]傅若秋,孟德胜,卢来春,等.高效液相色谱法测定薇依霜中维生素E和羟苯乙酯含量[J].中国药业,2012,21(7):12-13.
[9]徐文峰,徐硕,何笑荣,等.天然维生素E软胶囊中维生素E的含量测定[J].中国医药导报,2017,14(30):136-138,167.