纳米碳点对花生幼苗生长及其相关生理生化指标的影响
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摘要
在室内水培条件下,研究了不同质量浓度的纳米碳点对花生幼苗生长及相关生理生化指标的影响。结果表明,不同质量浓度的纳米碳点处理对花生种子萌发、幼苗生长、根系活力和抗氧化酶活性等均有不同程度的促进作用,且随着纳米碳点质量浓度的升高,花生种子的发芽势、发芽率、主根长、苗高、根干质量、地上部干质量、根系活力及幼苗的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性均表现出先升高后降低的趋势;此外,不同质量浓度的纳米碳点处理可降低花生幼苗中丙二醛(MDA)的含量,增加花生幼苗可溶性糖的含量。在各处理中,以纳米碳点质量浓度为180 mg/L时使用效果最佳,与CK相比,该处理花生幼苗的主根长、苗高、根干质量、地上部干质量、根鲜质量、地上部鲜质量、SOD活性、CAT活性分别比CK增加了36.84%,46.24%,31.03%,47.03%,55.93%,42.22%,22.73%,36.81%,而根系活力则提高了4.5倍。研究表明,适宜质量浓度的纳米碳点可以调节花生幼苗生理生化过程,促进幼苗的生长。
The effects of carbon nanodots with different concentrations on the seedling growth and related physiological and biochemical indexes of peanut were studied under the condition of indoor hydroponics. The results showed that the treatment of carbon nanodots had different promotion effects on seed germination, seedling growth, root activity and antioxidant enzyme activity. The germination potential, germination rate, main root length, seeding height, root dry mass, aboveground dry mass, root vigor, and the enzyme activities of superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) of seedlings all showed the same trend of increasing first and then decreasing along with the increase of carbon nanodots concentration. In addition, carbon nanodots reduced the content of malondialdehyde(MDA), and increased the content of soluble sugar in peanut seedlings. Compared with the control, the treatment with 180 mg/L carbon nanodots had the best effect, in which the main root length, seeding height, root dry mass, aboveground dry mass, root fresh weight, aboveground fresh weight, SOD activity, CAT activity and root vigor increased by 36.84%, 46.24%, 31.03%, 47.03%, 55.93%, 42.22%, 27.73%, 36.81% and 4.5 times, respectively. The results indicated that suitable concentration of carbon nanodots could promote the seedling growth of peanut by regulating the physiological and biochemical behaviors.
The effects of carbon nanodots with different concentrations on the seedling growth and related physiological and biochemical indexes of peanut were studied under the condition of indoor hydroponics. The results showed that the treatment of carbon nanodots had different promotion effects on seed germination, seedling growth, root activity and antioxidant enzyme activity. The germination potential, germination rate, main root length, seeding height, root dry mass, aboveground dry mass, root vigor, and the enzyme activities of superoxide dismutase(SOD), peroxidase(POD) and catalase(CAT) of seedlings all showed the same trend of increasing first and then decreasing along with the increase of carbon nanodots concentration. In addition, carbon nanodots reduced the content of malondialdehyde(MDA), and increased the content of soluble sugar in peanut seedlings. Compared with the control, the treatment with 180 mg/L carbon nanodots had the best effect, in which the main root length, seeding height, root dry mass, aboveground dry mass, root fresh weight, aboveground fresh weight, SOD activity, CAT activity and root vigor increased by 36.84%, 46.24%, 31.03%, 47.03%, 55.93%, 42.22%, 27.73%, 36.81% and 4.5 times, respectively. The results indicated that suitable concentration of carbon nanodots could promote the seedling growth of peanut by regulating the physiological and biochemical behaviors.
引文
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[2] Theodoros C,Athanasla K,Lamprini S,Apostolos A,Anastasios T,Constantine S.Two of a kind but different:Luminescent carbon quantum dots from citrus peels for iron and tartrazine sensing and cell imaging[J].Talanta,2017,175:305-312.doi:10.1016/j.talanta.2017.07.053.
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[4] Shen Y F,Juan S R,Markus R W,Axel H,Christian T,Lee J O,Heeg S,Hatting B,Reich S,Seki A,Seki S,Yoshida K,Sukkmaran S B,Helmuth M,Nakanish T.Assembly of carbon nanotubes and alkylated fullerenes:Nanocarbon hybrid towards photovoltaic applications[J].Chemical Science,2011,2(11):2243-2250.doi:10.1039/C1SC00360G.
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[6] 梁宏宇,胡迪,肖红梅.纳米技术在果蔬贮藏保鲜中的应用[J].保鲜与加工,2008,8(5):51-54.doi:10.3969/j.issn.1009-6221.2008.05.018.Liang H Y,Hu D,Xiao H M.Application of nanotechnology for preservation of fruit and vegetable[J].Storag and Process,2008,8(5):51-54.
[7] 谢寅峰,姚晓华.纳米TiO2对油松种子萌发及幼苗生长生理的影响[J].西北植物学报,2009,29(10):2013-2018.Xie Y F,Yao X H.Effects of nano-meter TiO2 on germination and growth physiology of pinus tabulae formis[J].Acta Agriculturae Boreali-Sinica,2009,29(10):2013-2018.
[8] 陆长梅,张超英,温俊强,吴国荣,陶明煊.纳米材料促进大豆萌芽、生长的影响及其机理研究[J].大豆科学,2002,21(3):168-171.doi:10.3969/j.issn.1000-9841.2002.03.002.Lu C M,Zhang C Y,Wen J Q,Wu G R,Tao M X.Research of the effect of nanomater materials on germination and growth enhancement of glycine max and its mechanism[J].Soybean Science,2002,21(3):168-171.
[9] 李大力,李丹,汪信.无机纳米粒子对人类细胞培养及植物组培苗影响的研究[J].江苏师范大学学报(自然科学版),2002,20(2):51-53.doi:10.3969/j.issn.1007-6573.2002.02.016.Li D L,Li D,Wang X.Effects of inorganic nanoparticles on human cells reproduction and bramble tissue culture[J].Journal of Jiangsu Normal University(Natural Science Edition),2002,20(2):51-53.
[10] 涂庆华,李娘辉,李玲.纳米化的二氧化钛促进绿豆下胚轴不定根形成[J].植物生理学报,2005,41(3):313-315.doi:10.13592/j.cnki.ppj.2005.03.010.Tu Q H,Li N H,Li L.The promotive effect of nanometer TiO2 on the adventitious rooting of mung bean hypocotyl[J].Plant Physlology Communications,2005,41(3):313-315.
[11] 王振红,罗专溪,颜昌宙,杨环清.纳米氧化锌对绿豆芽生长的影响[J].农业环境科学学报,2011,30(4):619-624.Wang Z H,Luo Z X,Yan C Z,Yang H Q.Effects of nano-ZnO particles on the growth of green bean sprouts[J].Journal of Agro-Environment Science,2011,30(4):619-624.
[12] 汪冰,丰伟悦,赵宇亮,邢更妹,柴之芳,王海芳,贾光.纳米材料生物效应及其毒理学研究进展[J].中国科学(化学),2005,35(1):1-10.doi:10.3321/j.issn:1006-9240.2005.01.001.Wang B,Feng W Y,Zhao Y L,Xing G M,Chai Z F,Wang H F,Jia G.Advances in biological effects and toxicology of nanomaterials[J].Scientia Sinica(Chimica),2005,35(1):1-10.
[13] Su M Y,Hong F S,Liu C,Wu X,Liu X Q,Chen L,Gao F Q,Yang F,Li Z R.Effects of nano-anatase TiO2 on absorption,distribution of light,and photoreduction activities of chloroplast membrane of spinach[J].Biological Trace Element Research,2007,118(2):120-130.doi:10.1007/s12011-007-0006-z.
[14] 刘娟,汤丰收,张俊,臧秀旺,董文召,易明林,郝西.国内花生生产技术现状及发展趋势研究[J].中国农学通报,2017,33(22):13-18.Liu J,Tang F S,Zhang J,Zang X W,Dong W Z,Yi M L,Hao X.Current status and development trends of peanut production technology in China[J].Chinese Agricultural Science Bulletin,2017,33(22):13-18.
[15] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.Li H S.Principle and technology of plant physiological and biochemical experiments[M].Beijing:Higher Education Press,2000.
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[17] 李明,王根轩.干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响[J].生态学报,2002,22(4):503-507.doi:10.3321/j.issn:1000-0933.2002.04.008.Li M,Wang G X.Effect of drought stress on activities of cell defense enzymes and lipid peroxidation in glycyrrhiza uralensis seedlings[J].Acta Ecologica Sinica,2002,22(4):503-507.
[18] 乔绍俊,李会珍,张志军,张鑫.盐胁迫对不同基因型紫苏种子萌发、幼苗生长和生理特征的影响[J].中国油料作物学报,2009,31(4):499-502.doi:10.3321/j.issn:1007-9084.2009.04.017.Qiao S J,Li H Z,Zhang Z J,Zhang X.Effect of salinity on seed germination,seedling growth and physiological changes in perilla frutescens[J].Chinese Journal of Oil Crop Sciences,2009,31(4):499-502.
[19] Khodakovskaya M,Dervishi E,Mahmood M,Xu Y,Li Z,Watanabe F,Biris A S.Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth[J].ACS Nano,2009,3(10):3221-3227.doi:10.1021/nn900887m.
[20] 周述波,贺立静,贺立红.纳米材料处理水对糯玉米生长及其生理变化的影响[J].玉米科学,2010,18(1):87-89,95.doi:10.13597/j.cnki.maize.science.2010.01.031.Zhou S B,He L J,He L H.Effects of treated water of nano devices on waxy corn growth and physiological changes[J].Journal of Maize Sciences,2010,18(1):87-89,95.
[21] 姜余梅,刘强,赵怡情,刘清岱,王芳,华泽田.碳纳米管对水稻种子萌发和根系生长的影响[J].湖北农业科学,2014(5):1010-1012.doi:10.14088/j.cnki.issn0439-8114.2014.05.039.Jiang Y M,Liu Q,Zhao Y Q,Liu Q D,Wang F,Hua Z T.Effects of carbon nanotubes on seed germination and root growth of rice[J].Hubei Agricultural Sciences,2014(5):1010-1012.
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