急性失血对机械通气大鼠血清神经特异性烯醇化酯和紧密联结蛋白5含量的影响
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摘要
目的探讨失血量对机械通气大鼠血清NSE、Claudin-5含量的影响。方法 Wistar大鼠30只,按失血量随机分为5组:对照组(C组)、失血20%组(S_2组)、失血40%组(S_4组)、失血60%组(S_6组)和失血80%组(S_8组),每组6只。各组大鼠麻醉后按组别于颈动脉放出相应血量,同时由股静脉行液体复苏。复苏3 h 55 min后行动脉血气分析,按氧分压和血红蛋白含量计算血氧含量。放血24 h后从颈动脉采血1 mL测定血清NSE和Claudin-5的含量。结果 1)与C组(18.8±1.0)相比,S_2、S_4、S_6、S_8组血液含氧量降低分别为14.6±0.7、10.2±0.6、7.9±0.3、6.3±0.4 mL/dL,且随着放血量的增加而逐级减少,S_8S_6>S_4>S_2组,P<0.05;S_4、S_6、S_8组血清Claudin-5含量均低于C组(P<0.05),S_8组最低(0.4±0.1)ng/mL,且S_8 Objective To evaluate the effect of acute blood loss on the NSE and Claudin-5 content in the serum of rats with mechanical ventilation. Methods Thirty Wistar rats, weighing 300—350 g, were randomly divided into 5 groups according to the volume of blood loss(n=6): group C, control group: no blood loss; group S_2, 20% of blood loss; group S_4, 40% of blood loss; group S_6, 60% of blood loss; group S_8, 80% of blood loss. After anesthesia, bloodletting was administered in each rat according to its corresponding groups from its carotid artery; fluid resuscitation was performed from the femoral vein in the meantime. 3 h 55 min after resuscitation, blood samples were collected immediately for artery blood gas analysis. Blood oxygen content was calculated according to oxygen partial pressure and hemoglobin content. After bleeding for 24 hours, 1 mL of blood were taken from carotid artery in each group for examining the contents of NSE and Claudin-5 in the serum. Results 1) Compared with group C, the contents of blood oxygen of the other groups were decreased(P<0.05). Group S8 was the lowest group(6.3±0.4)mL/dl. And the contents gradually decreased as the amount of blood loss increased: S_8S_6>S_4>S_2,(P<0.05). The levels of Claudin-5 in group S_4, S_6 and S_8 were lower than that in group C(P<0.05) with group S_8 being the lowest one(0.4±0.1) ng/mL and S_8
引文
[1] Rao G,Xie J,Hedrick A,et al.Hemorrhagic shock-induced cerebral bioenergetic imbalance is corrected by pharmacologic treatment with EF24 in a rat model.Neuropharmacology,2015,99:318-327.
[2] Sune N Jespersen,Leif ?stergaard.The roles of cerebral blood flow,capillary transit time heterogeneity,and oxygen tension in brain oxygenation and metabolism.J Cereb Blood Flow Metab,2012,32(2):264-277.
[3] Eve DJ,Steele MR,Sanberg PR,et al.Hyperbaric oxygen therapy as a potential treatment for post-traumatic stress disorder associated with traumatic brain injury.Neuropsychiatric Disease & Treatment,2016,12:2689-2705.
[4] Su L,Ying L,Shukun D,et al.Beneficial effects of hyperbaric oxygen on edema in rat hippocampus following traumatic brain injury.Experimental Brain Research,2015,233(12):3359-3365.
[5] Yang L,Li D,Chen S.Hydrogen water reduces NSE,IL-6,and TNF-α levels in hypoxic-ischemic encephalopathy.Open Medicine,2016,11(1):399-406.
[6] JunXiu LU,JingGuo C,HaiYan C.Diagnostic value of ultrasound and neuron-specific enolase and their correlation in premature infant with white matter damage.Journal of Clinical Pediatrics,2012,30(9):836-839.
[7] Zehendner CM,Librizzi L,Curtis MD,et al.Caspase-3 contributes to ZO-1 and Cl-5 tight-junction disruption in rapid anoxic neurovascular unit damage.Plos One,2011,6(2):e16760.
[8] Kaur C,Ling EA.Blood brain barrier in hypoxic-ischemic conditions.Curr Neurovasc Res,2008,5(1):71-81.
[9] MingHua Du,HongMin Luo,Sen Hu,et al.Electroacupuncture improves gut barrier dysfunction in prolonged hemorrhagic shock rats through vagus anti-in?ammatory mechanism.World J Gastroenterol,2013,19(36):5988-5999.
[10] Zetterberg H,Smith DH,Blennow K.Biomarkers of mild traumatic brain injury in cerebrospinal fluid and blood.Nature Reviews Neurology,2013,9(4):201-210.
[11] Calderon LM,Guyette FX,Doshi AA,et al.Combining NSE and S100B with clinical examination findings to predict survival after resuscitation from cardiac arrest.Resuscitation,2014,85(8):1025-1029.
[12] Szmydyngerchodobska J,Pascale CL,Pfeffer AN,et al.Expression of junctional proteins in choroid plexus epithelial cell lines:a comparative study.Cerebrospinal Fluid Research,2007,4(1):11.
[13] József Virág,Christine Haberler,Gábor Baksa,et al.Region specific differences of claudin-5 expression in pediatric intracranial ependymomas:potential prognostic role in supratentorial cases.Pathol Oncol Res,2017,23(2):245-252.
[14] Costine BA,Quebedaclerkin PB,Dodge CP,et al.Neuron-specific enolase,but not S100B or myelin basic protein,increases in peripheral blood corresponding to lesion volume after cortical impact in piglets.Journal of Neurotrauma,2012,29(17):2689-2695.
[15] Wen J,Qian S,Yang Q,et al.Overexpression of netrin-1 increases the expression of tight junction-associated proteins,claudin-5,occludin,and ZO-1,following traumatic brain injury in rats..Experimental & Therapeutic Medicine,2014,8(3):881-886.
[16] Xiaotang Ma,Huiting Zhang,Qunwen Pan,et al.Hypoxia/aglycemia-induced endothelial barrier dysfunction and tight junction protein downregulation can be ameliorated by citicoline.PLoS ONE,2013,8(12):e82604.
[17] Dong N,Diao Y,Ding M,et al.The effects of 7-nitroindazole on serum neuron-specific enolase and astroglia-derived protein (S100β) levels after traumatic brain injury.Experimental & Therapeutic Medicine,2017,13(6):3183-3188.
[2] Sune N Jespersen,Leif ?stergaard.The roles of cerebral blood flow,capillary transit time heterogeneity,and oxygen tension in brain oxygenation and metabolism.J Cereb Blood Flow Metab,2012,32(2):264-277.
[3] Eve DJ,Steele MR,Sanberg PR,et al.Hyperbaric oxygen therapy as a potential treatment for post-traumatic stress disorder associated with traumatic brain injury.Neuropsychiatric Disease & Treatment,2016,12:2689-2705.
[4] Su L,Ying L,Shukun D,et al.Beneficial effects of hyperbaric oxygen on edema in rat hippocampus following traumatic brain injury.Experimental Brain Research,2015,233(12):3359-3365.
[5] Yang L,Li D,Chen S.Hydrogen water reduces NSE,IL-6,and TNF-α levels in hypoxic-ischemic encephalopathy.Open Medicine,2016,11(1):399-406.
[6] JunXiu LU,JingGuo C,HaiYan C.Diagnostic value of ultrasound and neuron-specific enolase and their correlation in premature infant with white matter damage.Journal of Clinical Pediatrics,2012,30(9):836-839.
[7] Zehendner CM,Librizzi L,Curtis MD,et al.Caspase-3 contributes to ZO-1 and Cl-5 tight-junction disruption in rapid anoxic neurovascular unit damage.Plos One,2011,6(2):e16760.
[8] Kaur C,Ling EA.Blood brain barrier in hypoxic-ischemic conditions.Curr Neurovasc Res,2008,5(1):71-81.
[9] MingHua Du,HongMin Luo,Sen Hu,et al.Electroacupuncture improves gut barrier dysfunction in prolonged hemorrhagic shock rats through vagus anti-in?ammatory mechanism.World J Gastroenterol,2013,19(36):5988-5999.
[10] Zetterberg H,Smith DH,Blennow K.Biomarkers of mild traumatic brain injury in cerebrospinal fluid and blood.Nature Reviews Neurology,2013,9(4):201-210.
[11] Calderon LM,Guyette FX,Doshi AA,et al.Combining NSE and S100B with clinical examination findings to predict survival after resuscitation from cardiac arrest.Resuscitation,2014,85(8):1025-1029.
[12] Szmydyngerchodobska J,Pascale CL,Pfeffer AN,et al.Expression of junctional proteins in choroid plexus epithelial cell lines:a comparative study.Cerebrospinal Fluid Research,2007,4(1):11.
[13] József Virág,Christine Haberler,Gábor Baksa,et al.Region specific differences of claudin-5 expression in pediatric intracranial ependymomas:potential prognostic role in supratentorial cases.Pathol Oncol Res,2017,23(2):245-252.
[14] Costine BA,Quebedaclerkin PB,Dodge CP,et al.Neuron-specific enolase,but not S100B or myelin basic protein,increases in peripheral blood corresponding to lesion volume after cortical impact in piglets.Journal of Neurotrauma,2012,29(17):2689-2695.
[15] Wen J,Qian S,Yang Q,et al.Overexpression of netrin-1 increases the expression of tight junction-associated proteins,claudin-5,occludin,and ZO-1,following traumatic brain injury in rats..Experimental & Therapeutic Medicine,2014,8(3):881-886.
[16] Xiaotang Ma,Huiting Zhang,Qunwen Pan,et al.Hypoxia/aglycemia-induced endothelial barrier dysfunction and tight junction protein downregulation can be ameliorated by citicoline.PLoS ONE,2013,8(12):e82604.
[17] Dong N,Diao Y,Ding M,et al.The effects of 7-nitroindazole on serum neuron-specific enolase and astroglia-derived protein (S100β) levels after traumatic brain injury.Experimental & Therapeutic Medicine,2017,13(6):3183-3188.