高效液相色谱法同时测定水果中9种多酚物质
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摘要
目的以高效液相色谱法测定番石榴、番荔枝、大红柿中的没食子酸、儿茶素、表儿茶素、绿原酸、咖啡酸、阿魏酸、槲皮素、木犀草素、山奈酚共9种多酚类物质。方法用Diamosil(R)-C18(250 mm×4.6 mm,5μm)为分离柱,以甲醇和0.02%(V/V)甲酸水溶液(p H=3.0)为流动相进行梯度洗脱,流速为0.8 ml/min,进样量为10μl,柱温为35℃,二极管阵列检测器检测。结果 9种多酚类物质在40 min内完全分离,方法在0.25 mg/L~50 mg/L时线性良好,相关系数为0.999 8~0.999 9;检出限(S/N=3)为0.029 mg/L~0.18 mg/L;样品回收率为93.6%~101%,相对标准偏差为1.34%~6.99%。结论番石榴和番荔枝中检出酚类物质各6种,大红柿中检出酚类物质5种。该测定方法准确、灵敏,适用于番石榴、番荔枝、大红柿中9种多酚物质含量的检测。
Objective High performance liquid chromatography( HPLC) method was adopted to determine 9 types of polyphenols,including Gallic acid,( +)- Catechin,(-)- Epicatechin,Chlorogenic acid,Caffeic acid,Ferulic acid,Quercetin,Luteolin and Kaempferol in guava,cherimoya,and persimmon. Methods Diamosil( R)- C18( 250 mm × 4. 6 mm,5 μm)was adopted as the separation column; methanol and 0. 02%( V / V) formic acid solution( p H = 3. 0) were used as mobile phase for gradient elution. The flow rate was 0. 8 ml / min,the injection volume was 10 μl and the column temperature was 35 ℃.Diode array detector was used for detection. Results 9 types of polyphenols were separated completely within 40 min. It showed a good linearity when the method was performed within 0. 25 mg / L- 50 mg / L,and the correlation coefficient( r) was within 0. 999 8- 0. 999 9. The detection limit( S / N = 3) was within 0. 029 mg / L- 0. 18 mg / L. The recovery rate was within93. 6%- 101%,and the relative standard deviation( RSD) was within 1. 34%- 6. 99%. Conclusion 6 types of polyphenols were detected from guava and cherimoya,and 5 types of polyphenols were detected from persimmon. The determination method above is accurate and sensitive,and it is a suitable method to determine 9 types of polyphenols in guava,cherimoya and persimmon.
Objective High performance liquid chromatography( HPLC) method was adopted to determine 9 types of polyphenols,including Gallic acid,( +)- Catechin,(-)- Epicatechin,Chlorogenic acid,Caffeic acid,Ferulic acid,Quercetin,Luteolin and Kaempferol in guava,cherimoya,and persimmon. Methods Diamosil( R)- C18( 250 mm × 4. 6 mm,5 μm)was adopted as the separation column; methanol and 0. 02%( V / V) formic acid solution( p H = 3. 0) were used as mobile phase for gradient elution. The flow rate was 0. 8 ml / min,the injection volume was 10 μl and the column temperature was 35 ℃.Diode array detector was used for detection. Results 9 types of polyphenols were separated completely within 40 min. It showed a good linearity when the method was performed within 0. 25 mg / L- 50 mg / L,and the correlation coefficient( r) was within 0. 999 8- 0. 999 9. The detection limit( S / N = 3) was within 0. 029 mg / L- 0. 18 mg / L. The recovery rate was within93. 6%- 101%,and the relative standard deviation( RSD) was within 1. 34%- 6. 99%. Conclusion 6 types of polyphenols were detected from guava and cherimoya,and 5 types of polyphenols were detected from persimmon. The determination method above is accurate and sensitive,and it is a suitable method to determine 9 types of polyphenols in guava,cherimoya and persimmon.
引文
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[10]赵铁,金岩,姜清华.HPLC同时测定叶下珠中的槲皮素、木犀草素和山奈酚[J].华西药学杂志,2013,28(2):207-208.
[11]梁泰刚,刘伟,赵承孝,等.HPLC法同时测定养心草中没食子酸、槲皮素和山萘酚的含量[J].光谱实验室,2009,26(4):881-884.
[12]李岩,史晨杉,李田叶,等.HPLC法同时测定白刺果实中8种黄酮成分的含量[J].食品工业,2015,36(4):286-290.
[13]黄和,林晓霞,邝高波,等.番石榴多酚成分分析及抑菌活性研究[J].热带作物学报,2014,35(12):2482-2487.
[2]刘强,王亚强,许培仁,等.儿茶素类对h-VRS的体外抗菌活性研究[J].中国抗生素杂志,2011,36(7):557-561.
[3]Govea-Salas M,Rivas-Estilla AM,Rodríguez-Herrera R,et al.Gallic acid decreases hepatitis C virus expression through its antioxidant capacity[J].Exp Ther Med,2016,11(2):619-624.
[4]赵文红,邓泽元,范亚苇,等.阿魏酸体外抗氧化作用研究[J].食品科学,2010,31(1):219-223.
[5]高航,徐虹.植物多酚抗肥胖作用的研究进展[J].食品科学,2014,35(21):334-338.
[6]赵伟,李建科,何晓叶,等.几种常见植物多酚降血脂作用及机制研究进展[J].食品科学,2014,35(21):258-263.
[7]Fu L,Xu BT,Xu XR,et al.Antioxidant capacities and total phenolic contents of 62 fruits[J].Food Chem,2011,129(2):345-350.
[8]张元梅,周志钦,孙玉敬,等.高效液相色谱法同时测定柑橘果实中18种类黄酮的含量[J].中国农业科学,2012,45(17):3558-3565.
[9]徐维盛,李东,刘静,等.果酒中11种酚类化合物反相高效液相色谱检测方法的建立[J].卫生研究,2013,42(3):497-503,528.
[10]赵铁,金岩,姜清华.HPLC同时测定叶下珠中的槲皮素、木犀草素和山奈酚[J].华西药学杂志,2013,28(2):207-208.
[11]梁泰刚,刘伟,赵承孝,等.HPLC法同时测定养心草中没食子酸、槲皮素和山萘酚的含量[J].光谱实验室,2009,26(4):881-884.
[12]李岩,史晨杉,李田叶,等.HPLC法同时测定白刺果实中8种黄酮成分的含量[J].食品工业,2015,36(4):286-290.
[13]黄和,林晓霞,邝高波,等.番石榴多酚成分分析及抑菌活性研究[J].热带作物学报,2014,35(12):2482-2487.