调心方对阿尔茨海默病小鼠模型早期学习记忆能力及LTP的影响
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摘要
目的观察调心方对阿尔茨海默病早期APP/PS1双转基因小鼠学习记忆能力及海马长时程增强(LTP)的影响。方法以3月龄APP/PS1双转基因小鼠30只为动物模型,随机分为模型组、中药组、西药组,各10只。另选取C57野生型小鼠8只作为对照组。采用Morris水迷宫观察小鼠学习记忆能力、电生理记录小鼠海马CA1区LTP变化。结果与对照组比较,模型组小鼠学习记忆能力明显下降,LTP受抑制,差异均有统计学意义(P<0.05或0.01);与模型组比较,中药组、西药组小鼠学习记忆能力均明显提高,差异均有统计学意义(P<0.05),中药组LTP水平升高,差异有统计学意义(P<0.05或0.01),西药组LTP水平无变化,差异无统计学意义(P>0.05)。结论中药方剂调心方能够改善3月龄APP/PS1双转基因小鼠学习记忆能力及海马区LTP水平,对阿尔茨海默病小鼠模型早期表现学习认知障碍具有改善作用。
Objective To observe the effect of heart benefiting recipe on learning and memory ability of early APP/PS1 mice in alzheimer′ s disease and the long-term potentiation(LTP)of the hippocampus.Methods Thirty 3 month old APP/PS1 double transgenic mice were taken as animal models,and they were randomly divided intomodel group,Chinese medicine group and Western medicine group,each 10 in group. The C57 wild type mice were selected as control group.The ability of learning and memory of mice was observed by Morris water maze,and the changes of LTP in the hippocampus CA1 region was recorded by electrophysiological. Results Compared with the blank group,the learning and memory abilities of the model group mice were obviously decreased,and the LTP was inhibited,the difference were statistically significant(P<0.05 or 0.01).Compared with the model group,the learning and memory abilities of the Chinese medicine group and Western medicine group mice were obviously increased,the difference were statistically significant(P<0.05),and the level of LTP in Chinese medicine group was increased,the difference were statistically significant(P<0.01 or 0.05).There was no significant difference in LTP levels between model group and Western medicine group(P>0.05).Conclusions The Heart Benefiting Recipe can improve the learning and memory ability and the LTP level of hippocampus in 3 month old APP/PS1 double transgenic mice,also can improve the cognitive disorder of early stage of Alzheimer's disease mices.
Objective To observe the effect of heart benefiting recipe on learning and memory ability of early APP/PS1 mice in alzheimer′ s disease and the long-term potentiation(LTP)of the hippocampus.Methods Thirty 3 month old APP/PS1 double transgenic mice were taken as animal models,and they were randomly divided intomodel group,Chinese medicine group and Western medicine group,each 10 in group. The C57 wild type mice were selected as control group.The ability of learning and memory of mice was observed by Morris water maze,and the changes of LTP in the hippocampus CA1 region was recorded by electrophysiological. Results Compared with the blank group,the learning and memory abilities of the model group mice were obviously decreased,and the LTP was inhibited,the difference were statistically significant(P<0.05 or 0.01).Compared with the model group,the learning and memory abilities of the Chinese medicine group and Western medicine group mice were obviously increased,the difference were statistically significant(P<0.05),and the level of LTP in Chinese medicine group was increased,the difference were statistically significant(P<0.01 or 0.05).There was no significant difference in LTP levels between model group and Western medicine group(P>0.05).Conclusions The Heart Benefiting Recipe can improve the learning and memory ability and the LTP level of hippocampus in 3 month old APP/PS1 double transgenic mice,also can improve the cognitive disorder of early stage of Alzheimer's disease mices.
引文
[1]林水淼,周如倩,王健,等.调心方、补肾方对Alzheimer病患者认知功能和日常生活能力作用的比较研究[J].中国老年学杂志,2002,22(6):434-436.
[2]王健,林水淼,周如倩,等.调心方治疗阿尔茨海默氏病的临床研究[J].北京中医药大学学报,2002,25(3):51-53.
[3]刘学源,赵伟康,徐品初,等.调心方对杏仁核注射Aβ25-35诱导的阿尔茨海默病模型大鼠的影响[J].中草药,2004,35(1):50-53.
[4]周晖,赵伟康,金国琴.调心方对Aβ大鼠痴呆模型脑组织IL-1β、IL-6及APPm RNA表达的影响[J].中国中西医结合杂志,2004,24(6):529-533.
[5]MORRIS R.Developments of a water-maze procedure for studying spatial learning in the rat[J].J Neurosci Methods,1984,11(1):47-60.
[6]黄晏,杨胜,周文霞,等.小鼠在体海马长时程增强记录技术[J].中国应用生理学杂志,2008,24(3):291-295.
[7]BERTOLINO A,NAWROZ S,MATTAY VS,et al.Regionally specific pattern of neurochemical pathology in schizophrenia as assessed by multislice proton magnetic resonance spectroscopic imaging[J].Am J Psychiatry,1996,153(12):1554-1563.
[8]SHAPIRO M.Plasticity,hippocampal place cells,and cognitive maps[J].Arch Neurol,2001,58(6):874-881.
[9]ABRAHAM WC,WILLIAMS JM.Properties and mechanisms of LTP maintenance[J].Neuroscientist,2003,9(6):463-474.
[10]TRINCHESE F,LIU S,BATTAGLIA F,et al.Progressive age-related developmentof Alzheimer-likepathologyin APP/PS1mice[J].Ann Neurol,2004,55(6):801-814.
[11]WIRTHS O,WEIS J,SZCZYGIELSKI J,et al.Axonopathy in an APP/PS1 transgenic mouse model of Alzheimer′s disease[J].Acta Neuropathol,2006,111(4):312-319.
[12]BLANCHARD V,MOUSSAOUI S,CZECH C,et al.Time sequence of maturation of dystrophic neurites associated with Abeta deposits in APP/PS1 transgenic mice[J].Exp Neurol,2003,184(1):247-263.
[2]王健,林水淼,周如倩,等.调心方治疗阿尔茨海默氏病的临床研究[J].北京中医药大学学报,2002,25(3):51-53.
[3]刘学源,赵伟康,徐品初,等.调心方对杏仁核注射Aβ25-35诱导的阿尔茨海默病模型大鼠的影响[J].中草药,2004,35(1):50-53.
[4]周晖,赵伟康,金国琴.调心方对Aβ大鼠痴呆模型脑组织IL-1β、IL-6及APPm RNA表达的影响[J].中国中西医结合杂志,2004,24(6):529-533.
[5]MORRIS R.Developments of a water-maze procedure for studying spatial learning in the rat[J].J Neurosci Methods,1984,11(1):47-60.
[6]黄晏,杨胜,周文霞,等.小鼠在体海马长时程增强记录技术[J].中国应用生理学杂志,2008,24(3):291-295.
[7]BERTOLINO A,NAWROZ S,MATTAY VS,et al.Regionally specific pattern of neurochemical pathology in schizophrenia as assessed by multislice proton magnetic resonance spectroscopic imaging[J].Am J Psychiatry,1996,153(12):1554-1563.
[8]SHAPIRO M.Plasticity,hippocampal place cells,and cognitive maps[J].Arch Neurol,2001,58(6):874-881.
[9]ABRAHAM WC,WILLIAMS JM.Properties and mechanisms of LTP maintenance[J].Neuroscientist,2003,9(6):463-474.
[10]TRINCHESE F,LIU S,BATTAGLIA F,et al.Progressive age-related developmentof Alzheimer-likepathologyin APP/PS1mice[J].Ann Neurol,2004,55(6):801-814.
[11]WIRTHS O,WEIS J,SZCZYGIELSKI J,et al.Axonopathy in an APP/PS1 transgenic mouse model of Alzheimer′s disease[J].Acta Neuropathol,2006,111(4):312-319.
[12]BLANCHARD V,MOUSSAOUI S,CZECH C,et al.Time sequence of maturation of dystrophic neurites associated with Abeta deposits in APP/PS1 transgenic mice[J].Exp Neurol,2003,184(1):247-263.