基于核酸适配体的上转换荧光纸基传感器用于测定茶水中的黄曲霉毒素B1

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英文篇名：Lateral flow aptamer assay for detection of aflatoxin B1 in tea using upconversion nanoparticles 作者：金碧瑞 ; 杨叶欣 ; 卢天健 ; 徐峰 ; 林敏 英文作者：Birui Jin;Yexin Yang;Tianjian Lu;Feng Xu;Min Lin;State Key Laboratory for Mechanical Behavior of Materials, School of Materials Science and Engineering, Xi'an Jiaotong University;Bioinspired Engineering and Biomechanics Center(BEBC), Xi'an Jiaotong University;The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University;Bioinspired Engineering and Biomechanics Center (BEBC), Xi'an Jiaotong University;MOE Key Laboratory of Multifunctional Materials and Structures, Ministry of Education, Xi'an Jiaotong University; 关键词：黄曲霉毒素B1 ; 上转换纳米颗粒 ; 核酸适配体 ; 侧流试纸 英文关键词：aflatoxin B1;;upconversion nanoparticle;;aptamers;;lateral flow assays 中文刊名：JBXK 英文刊名：Scientia Sinica(Chimica) 机构：西安交通大学材料力学性能国家重点实验室;西安交通大学仿生工程与生物力学中心;西安交通大学生命科学与技术学院生物信息工程教育部重点实验室;西安交通大学多功能材料与结构教育部重点实验室; 出版日期：2019-02-20 出版单位：中国科学:化学 年：2019 期：v.49 基金：陕西省创新人才推进计划(编号:2017KJXX-28);; 中央高校基本科研业务费专项资金(编号:2016qngz03);; 航天营养与食品工程重点实验室开放基金(编号:SNFE-KF-15-09);; 陕西省创新团队(编号:2017KCT-22);; 榆林市重点科技创新团队(编号:2017KJJH-02)资助项目 语种：中文; 页：JBXK201902013 页数：7 CN：02 ISSN：11-5838/O6 分类号：147-153

摘要

黄曲霉毒素大量存在于花生、玉米、稻米、大豆、小麦和茶叶中,其毒性、致癌力在霉菌毒素中都居首位,是对人类健康危害极为突出的一类霉菌毒素.本研究使用上转换纳米材料为荧光标记物,在其表面修饰可特异性地结合黄曲霉毒素B1的核酸适配体,构建出一种基于竞争法的荧光侧流试纸检测技术.该技术可实现对黄曲霉毒素B1的快速检测,在0.1～100 ng/mL的浓度范围内具有良好的线性度,最低检出限为0.03 ng/mL,并实现了对实际茶水样品的定量检测.该方法灵敏度高、特异性强、适用于现场的快速检测,具有良好的经济价值和应用前景.
        Aflatoxin, extensively existing in peanuts, corn, rice, soybeans, wheat and tea, is a type of mycotoxins with the highest toxicity and carcinogenicity and thus poses a serious threat to human health. In this article, upconversion nanoparticles modified with aptamers specific to aflatoxin B1 were used as fluorescence markers. By this way, we constructed a paper-based lateral flow assays detection technology for rapid detection of aflatoxin B1 with good linearityin the concentration range of 0.1–100 ng/mL and detection limit of 0.03 ng/mL. This method can realize quantitative detection of aflatoxin B1 in real tea sample with high sensitivity and specificity. Hence it is suitable for rapid on-site detection of aflatoxin B1 by utilizing this method, which shows good economic value and application prospects.

引文

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