单核细胞增生李斯特菌的国际性能力验证实践
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摘要
为了验证实验室对单核细胞增生李斯特菌的检测能力,通过分析讨论能力验证过程,为提升实验室各类食源性微生物的检测能力、获得满意的能力验证结果提供重要的参考依据。依据英国FAPAS分析实验室提供的能力验证说明书、GB 4789.30—2016 《食品安全国家标准食品微生物学检验单核细胞增生李斯特菌检验》方法进行检验,利用mini-VIDAS全自动酶联荧光免疫系统、VITEK 2compact全自动微生物快速检测分析系统、NEW ATB细菌鉴定药物敏感试验、BAX System Q7全自动病原微生物快速检测方法进行筛选和鉴定。结果显示,样品M229d02-A检出单核细胞增生李斯特菌,检出李斯特菌属,样品M229d02-B未检出单核细胞增生李斯特菌,检出李斯特菌属。检测结果与英国FAPAS分析实验室公布的结果一致。本次能力验证有效地验证了实验室单核细胞增生李斯特菌的检测能力,同时也验证了实验室在食源性微生物检验检测领域的技术能力及各类检测技术的有效应用。
To verify the laboratory's ability to detect Listeria Monocytogenes, through analyzing and discussing the process of proficiency verification, provide an important reference for improving the detection ability of various food-borne microorganisms in laboratory and obtaining satisfactory proficiency verification results. According to the proficiency certification instructions provided by FAPAS analytical laboratory in UK, adopt GB 4789.30—2016 "National Food Safety Standard Food Microbiological Examination: Listeria Monocytogenes to inspect". Using mini-VIDAS full-automatic ELISA system, VITEK 2 compact full-automatic microbial rapid detection and analysis system, NEW ATB bacterial identification drug sensitivity test,BAX System Q7 full-automatic rapid detection method of pathogenic microorganisms for screening and identification. The result shows thatListeria Monocytogenes and Listeria were detected in sample M229 d02-A,Listeria Monocytogenes was not detected in sample M229 d02-B, Listeria was detected in sample M229 d02-B.The results are consistent with those published by FAPAS Analytical Laboratory in Britain.This capability validation validates the detection ability of Listeria Monocytogenes in the laboratory, and also validates the technical ability of the laboratory in the field of food-borne microorganism detection and the effective application of various detection techniques.
To verify the laboratory's ability to detect Listeria Monocytogenes, through analyzing and discussing the process of proficiency verification, provide an important reference for improving the detection ability of various food-borne microorganisms in laboratory and obtaining satisfactory proficiency verification results. According to the proficiency certification instructions provided by FAPAS analytical laboratory in UK, adopt GB 4789.30—2016 "National Food Safety Standard Food Microbiological Examination: Listeria Monocytogenes to inspect". Using mini-VIDAS full-automatic ELISA system, VITEK 2 compact full-automatic microbial rapid detection and analysis system, NEW ATB bacterial identification drug sensitivity test,BAX System Q7 full-automatic rapid detection method of pathogenic microorganisms for screening and identification. The result shows thatListeria Monocytogenes and Listeria were detected in sample M229 d02-A,Listeria Monocytogenes was not detected in sample M229 d02-B, Listeria was detected in sample M229 d02-B.The results are consistent with those published by FAPAS Analytical Laboratory in Britain.This capability validation validates the detection ability of Listeria Monocytogenes in the laboratory, and also validates the technical ability of the laboratory in the field of food-borne microorganism detection and the effective application of various detection techniques.
引文
[1]朱静鸿,龚云伟,武艳力.食品中单核细胞增生李斯特菌污染状况调查[J].中国卫生工程学,2016,15(5):491-493.
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[3]芦丹,刘国蓉,李宏通,等.北京市昌平区2010-2015年市售食品中单核细胞增生李斯特菌污染状况分析[J].实用预防医学,2018,25(3):264-266.
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[5]Mead PS,Slutsker L,Dietz V,et al.Food-Related Illness and Death in the United States[J].Emerg Infect Dis,1999,5(5):607-625.
[6]沈托,焦莉萍,李通情,等.2011-2015年渭南市市售食品中单核细胞增生李斯特菌污染状况监测与分析[J].实用预防医学,2018,25(1):66-69.
[7]张淑红,侯凤伶,关文英,等.2005-2013年河北省即食食品中单增李斯特菌污染及耐药特征研究[J].中国食品卫生杂志,2014,26(6):596-599.
[8]李云霞,陈雯雯,顾晨荣,等.单增李斯特菌的检测方法研究进展[J].上海师范大学学报(自然科学版),2015,44(6):687-693.
[9]李翠萍.食品中单核细胞增生李斯特菌检测的能力验证[J].中国城乡企业卫生,2017,32(10):41-42.
[10]孙玉霜,李颖,李秀梅,等.食源性单增李斯特菌检测技术研究进展[J].中国动物检疫,2017,34(8):76-80.
[11]张玲,陈曦,罗丽娟,等.一起单增李斯特菌引起新生儿败血症的溯源研究[J/OL].疾病监测,2018(11):936-939.[2018-12-04].http://sso.gzlib.gov.cn/interlibSSO/goto/75/=jmr9bmjh9mds/kcms/detail/11.2928.R.20181113.1514.008.html.
[12]DOC.Food-borne diseases active surveillance network(Food Net)[EB/OL].https://www.cdc.gov/food net/pdfs/FoodNet-AnnualReport-2015-508c.pdf,2017-03-02/2018-10-04.
[13]陈伟伟,吴晓敏,廖冬冬,等.2016年福建省单核细胞增生李斯特菌临床病例及食品来源菌株的分子特征分析[J/OL].中国人兽共患病学报:1-6.[2018-11-02].http://kns.cnki.net/kcms/detail/35.1284.r.20181031.1412.004.html.
[14]乌日娜,刘翔,郭邦成,等.银川市市售食品中单增李斯特菌污染状况分析研究[J].安徽农业科学,2014,42(18):5958-5961.
[15]陈洁,周剑,郑露.食品中单核细胞增生李斯特氏菌检测能力验证分析[J].河南预防医学杂志,2018,29(11):832-837.
[16]GB 4789.30-2016食品安全国家标准食品微生物学检验单核细胞增生李斯特氏菌检验[S].
[17]张昕,汪琦,张惠媛,等.检测致病性李斯特氏菌的培养基[J].检验检疫科学,2008(1):76-79.
[18]任广睦,刘季,王英元.实时荧光定量PCR技术应用于核酸定量检测的研究进展及展望[J].山西医科大学学报,2006(9):973-976.
[19]Wall SJ,Edw ard DR.Quantitative Reverse Transcription-Polymerase Chain Reaction(RT-PCR):A Comparison of Prime-dropping,competitive,and real-time RT-PCRs[J].Anal Biochem,2002,300(2):269-273.
[20]S chmittgen TD.Real-Time Quantit Ative PCR[J].Methods,2001,25(4):383-385.
[21]Felkin LE,Taegtmeyer AB,Bart on PJ.Real-Time Quantitati Vepolymerase Chain Reaction in Cardiac Transplant Research[J].Methods MolBiol,2006,333:305-330.
[22]韩俊英,曾瑞萍.荧光定量PCR技术及其应用[J].国外医学:遗传学分册,2000,23(3):177.
[23]安钢力.实时荧光定量PCR技术的原理及其应用[J/OL].中国现代教育装备,2018(23):1-2.[2018-12-01].http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CFJD&dbname=CJFDLAST2019&filename=JYSB201821009&v=MjM3MDhIOW5Pcm85RmJZUjhlWDFMd XhZUzdEaDFUM3FUcldNMUZyQ1VSTE9mWk9adUZ5emdXcjdPTHpUWWJMRzQ=.
[24]徐潇,林兰,崔生辉,等.食品中致病菌常用检测技术分析[J].中国药事,2012,26(2):185-190.
[25]肖莉,左泽彦.实时定量PCR检测食品中单核细胞增生性李斯特氏菌[J].山东化工,2016,45(17):84-85+87.
[26]马晓燕,张会彦,贾春凤,等.荧光定量PCR检测单核细胞增生性李斯特氏菌[J].安徽农业科学,2011,39(15):9274-9276.
[27]Sewell AM,Warburton DW,Boville A,et al.The Development of an Efficient and Rapid Enzyme Linked Fluorescent Assay Method for the Detection of Listeria spp.from foods[J].International Journal of Food Microbiology,2003,81(2):123-129.
[2]斯国静,俞骅,王一泓.杭州市市售肉类食品中单增李斯特菌污染状况调查研究[J].中国卫生检验杂志,2009,19(2):408-409.
[3]芦丹,刘国蓉,李宏通,等.北京市昌平区2010-2015年市售食品中单核细胞增生李斯特菌污染状况分析[J].实用预防医学,2018,25(3):264-266.
[4]周良君,陈果,江智辉,等.2010年绵阳市食品中单增李斯特菌的污染状况及耐药性监测[J].职业与健康,2011,27(20):2330-2332.
[5]Mead PS,Slutsker L,Dietz V,et al.Food-Related Illness and Death in the United States[J].Emerg Infect Dis,1999,5(5):607-625.
[6]沈托,焦莉萍,李通情,等.2011-2015年渭南市市售食品中单核细胞增生李斯特菌污染状况监测与分析[J].实用预防医学,2018,25(1):66-69.
[7]张淑红,侯凤伶,关文英,等.2005-2013年河北省即食食品中单增李斯特菌污染及耐药特征研究[J].中国食品卫生杂志,2014,26(6):596-599.
[8]李云霞,陈雯雯,顾晨荣,等.单增李斯特菌的检测方法研究进展[J].上海师范大学学报(自然科学版),2015,44(6):687-693.
[9]李翠萍.食品中单核细胞增生李斯特菌检测的能力验证[J].中国城乡企业卫生,2017,32(10):41-42.
[10]孙玉霜,李颖,李秀梅,等.食源性单增李斯特菌检测技术研究进展[J].中国动物检疫,2017,34(8):76-80.
[11]张玲,陈曦,罗丽娟,等.一起单增李斯特菌引起新生儿败血症的溯源研究[J/OL].疾病监测,2018(11):936-939.[2018-12-04].http://sso.gzlib.gov.cn/interlibSSO/goto/75/=jmr9bmjh9mds/kcms/detail/11.2928.R.20181113.1514.008.html.
[12]DOC.Food-borne diseases active surveillance network(Food Net)[EB/OL].https://www.cdc.gov/food net/pdfs/FoodNet-AnnualReport-2015-508c.pdf,2017-03-02/2018-10-04.
[13]陈伟伟,吴晓敏,廖冬冬,等.2016年福建省单核细胞增生李斯特菌临床病例及食品来源菌株的分子特征分析[J/OL].中国人兽共患病学报:1-6.[2018-11-02].http://kns.cnki.net/kcms/detail/35.1284.r.20181031.1412.004.html.
[14]乌日娜,刘翔,郭邦成,等.银川市市售食品中单增李斯特菌污染状况分析研究[J].安徽农业科学,2014,42(18):5958-5961.
[15]陈洁,周剑,郑露.食品中单核细胞增生李斯特氏菌检测能力验证分析[J].河南预防医学杂志,2018,29(11):832-837.
[16]GB 4789.30-2016食品安全国家标准食品微生物学检验单核细胞增生李斯特氏菌检验[S].
[17]张昕,汪琦,张惠媛,等.检测致病性李斯特氏菌的培养基[J].检验检疫科学,2008(1):76-79.
[18]任广睦,刘季,王英元.实时荧光定量PCR技术应用于核酸定量检测的研究进展及展望[J].山西医科大学学报,2006(9):973-976.
[19]Wall SJ,Edw ard DR.Quantitative Reverse Transcription-Polymerase Chain Reaction(RT-PCR):A Comparison of Prime-dropping,competitive,and real-time RT-PCRs[J].Anal Biochem,2002,300(2):269-273.
[20]S chmittgen TD.Real-Time Quantit Ative PCR[J].Methods,2001,25(4):383-385.
[21]Felkin LE,Taegtmeyer AB,Bart on PJ.Real-Time Quantitati Vepolymerase Chain Reaction in Cardiac Transplant Research[J].Methods MolBiol,2006,333:305-330.
[22]韩俊英,曾瑞萍.荧光定量PCR技术及其应用[J].国外医学:遗传学分册,2000,23(3):177.
[23]安钢力.实时荧光定量PCR技术的原理及其应用[J/OL].中国现代教育装备,2018(23):1-2.[2018-12-01].http://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CFJD&dbname=CJFDLAST2019&filename=JYSB201821009&v=MjM3MDhIOW5Pcm85RmJZUjhlWDFMd XhZUzdEaDFUM3FUcldNMUZyQ1VSTE9mWk9adUZ5emdXcjdPTHpUWWJMRzQ=.
[24]徐潇,林兰,崔生辉,等.食品中致病菌常用检测技术分析[J].中国药事,2012,26(2):185-190.
[25]肖莉,左泽彦.实时定量PCR检测食品中单核细胞增生性李斯特氏菌[J].山东化工,2016,45(17):84-85+87.
[26]马晓燕,张会彦,贾春凤,等.荧光定量PCR检测单核细胞增生性李斯特氏菌[J].安徽农业科学,2011,39(15):9274-9276.
[27]Sewell AM,Warburton DW,Boville A,et al.The Development of an Efficient and Rapid Enzyme Linked Fluorescent Assay Method for the Detection of Listeria spp.from foods[J].International Journal of Food Microbiology,2003,81(2):123-129.