硫酸化茯苓多糖对MPTP诱导的小鼠多巴胺能神经元损伤的保护作用
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摘要
目的:探讨硫酸化茯苓多糖(SP)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导小鼠黑质多巴胺(DA)能神经元的保护作用。方法:ICR小鼠随机分为5组:对照组、MPTP模型组和SP治疗组(50、100、150 mg/kg),实施腹腔注射给药。爬杆行为评价各组小鼠的运动能力,酪氨酸羟化酶(TH)免疫染色法和TUNEL法检测黑质神经元的损伤与凋亡情况,紫外分光光度法检测小鼠中脑和纹状体中DA含量、纹状体中一氧化氮合酶(NOS)活性。结果:MPTP组中小鼠的爬杆时间显著延长,TH阳性细胞明显减少,TUNEL阳性细胞数量增加,中脑和纹状体中的多巴胺含量显著下降(P<0.01),纹状体中NOS活性显著上升(P<0.01)。SP治疗组中,MPTP导致的上述变化均得到改善:小鼠爬杆时间缩短,TH阳性细胞增多,TUNEL阳性细胞减少;小鼠中脑和纹状体DA含量显著上升(P<0.01),NOS活性明显下降(P<0.05)。结论:SP对MPTP诱导的帕金森小鼠黑质DA能神经元具有一定的保护作用。
AIM: To explore the protective effect of sulfated pachymaran( SP) on MPTP- induced damage in mouse substantia nigra neurons.METHODS: ICR mice were randomly divided into five groups: control group,MPTP model group and SP group( 50,100,150 mg / kg),and all drugs were administrated to mice by abdominal injection.Pole test was performed to assess the ability of moving. TH immunostaining and TUNEL labeling were used to observe the damage and apoptosis of nigral neurons. The levels of dopamine( DA),malondialdehvde( MDA) and hydrogen peroxide( H2O2) in both midbrain and striatum were detected by UV spectrophotometry. RESULTS: Compared with the control group,the mice in MPTP model group took longer time to descend; TH-positive neurons and the level of DA in midbrain and striatum decreased obviously,and TUNEL-positive neurons increased significantly,but the level of H2O2 in midbrain and striatum did not vary significantly. Compared with the model group,SP treatment was shown to reverse all the above changes induced by MPTP. CONCLUSION: SP may have protective effects on MPTP-induced damage of dopaminergic neurons in Parkinsons' disease PD mice.
AIM: To explore the protective effect of sulfated pachymaran( SP) on MPTP- induced damage in mouse substantia nigra neurons.METHODS: ICR mice were randomly divided into five groups: control group,MPTP model group and SP group( 50,100,150 mg / kg),and all drugs were administrated to mice by abdominal injection.Pole test was performed to assess the ability of moving. TH immunostaining and TUNEL labeling were used to observe the damage and apoptosis of nigral neurons. The levels of dopamine( DA),malondialdehvde( MDA) and hydrogen peroxide( H2O2) in both midbrain and striatum were detected by UV spectrophotometry. RESULTS: Compared with the control group,the mice in MPTP model group took longer time to descend; TH-positive neurons and the level of DA in midbrain and striatum decreased obviously,and TUNEL-positive neurons increased significantly,but the level of H2O2 in midbrain and striatum did not vary significantly. Compared with the model group,SP treatment was shown to reverse all the above changes induced by MPTP. CONCLUSION: SP may have protective effects on MPTP-induced damage of dopaminergic neurons in Parkinsons' disease PD mice.
引文
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[14]高贵珍,吴超,陈哲,等.硫酸化茯苓多糖对MPP+诱导的PC12细胞损伤的保护作用研究[J].淮北师范大学学报,2015,36(4):52-58.
[15]许璟,徐盛权,梁洁,等.尼克酰胺对帕金森病模型小鼠的保护作用[J].浙江大学学报(医学版),2012,41(2):146-152.
[2]Yong-Kee CJ,Warre R,Monnier PP,et al.Evidence for synergism between cell death mechanisms in a cellular model of neurodegeneration in Parkinson's disease[J].Neurotox Res,2012,22(4):355-364.
[3]Salat D,Tolosa E.Levodopa in the treatment of Parkinson's disease:current status and new developments[J].J Parkin Dis,2013,3(3):255-269.
[4]Obeso JA,Rodriguez-Oroz MC,Benitez-Temino B,et al.Functional organization of the basal ganglia:therapeutic implications for Parkinson's disease[J].Mov Disord,2008,23(Suppl 3):S548-S559.
[5]Wang X,Wang J,Zhang J,et al.Structure-antioxidant relationships of sulfated galactomannan from guar gum[J].Int J Biol Macromol,2010,46(1):59-66.
[6]李佳青,李文妍,银金兰.治疗帕金森病药物的研究进展[J].中国临床医生,2012,40(12):24-26.
[7]张秀丽,李亚晨,牛新华,等.原儿茶酸对帕金森模型鼠脑组织抗氧化能力的影响[J].现代生物医学进展,2011,11(17):3248-3251.
[8]牛爽,郝利民,赵树欣,等.茯苓多糖的研究进展[J].食品科学,2012,9(13):348-353.
[9]梅天笑,王小莺,万根,等.多糖的修饰及其抗凝血活性研究进展[J].江西农业大学学报,2013,35(5):1108-1113.
[10]陈小红,马芳,严云.茯苓多糖的结构改性及抗氧化活性研究[J].湖北农业科学,2015,54(17):4563-4566.
[11]高贵珍,孙冰清,吴超,等.硫酸化茯苓多糖对小鼠腹腔巨噬细胞功能的影响[J].安徽工程大学学报,2015,30(1):1-5.
[12]高贵珍,吴超,单玲玲,等.硫酸化茯苓多糖对帕金森模型鼠脑组织抗氧化能力的影响[J].中国临床药理学与治疗学,2016,21(4):384-388.
[13]高贵珍,汪俊博,姜芳,等.硫酸化茯苓多糖对MPTP诱导帕金森小鼠的神经保护作用研究[J].中国药理学通报,2015,31(12):1699-1704.
[14]高贵珍,吴超,陈哲,等.硫酸化茯苓多糖对MPP+诱导的PC12细胞损伤的保护作用研究[J].淮北师范大学学报,2015,36(4):52-58.
[15]许璟,徐盛权,梁洁,等.尼克酰胺对帕金森病模型小鼠的保护作用[J].浙江大学学报(医学版),2012,41(2):146-152.