硫酸化茯苓多糖对帕金森模型鼠脑组织抗氧化能力的影响
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摘要
目的:考察硫酸化茯苓多糖(SP)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的帕金森小鼠脑组织抗氧化能力的影响。方法:MPTP注射ICR小鼠制备帕金森病(PD)模型。将ICR小鼠随机分为5组:对照组、MPTP组和SP治疗组(50,100,150 mg/kg),腹腔注射给药。紫外分光光度法检测小鼠纹状体中超氧化物岐化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、乳酸脱氢酶(LDH)的活性和抗超氧阴离子活力以及过氧化氢(H_2O_2)、丙二醛(MDA)的含量,HE染色观察小鼠脑组织形态学变化。结果:与模型组相比,在一定浓度范围内,SP治疗组小鼠纹状体GSH-Px、SOD、CAT、LDH活性及抗超氧阴离子活力有不同程度的升高,H_2O_2和MDA含量有不同程度的下降;海马区神经元排列密集有序,细胞间隙减小,神经元细胞形态结构有不同程度的恢复。结论:SP对MPTP诱导的神经元损伤具有神经保护作用,其潜在机制可能是提高脑组织抗氧化能力,减轻氧化应激反应。
AIM: To investigate the effects of sulfated pachymaran( SP) on cerebral tissue antioxidant indexes in model mice of Parkinsons ' disease( PD) induced by MPTP. METHODS: ICR mice were treated with MPTP to make PD model. The mice were randomly divided into five groups: control group,MPTP model group and SP group( 50,100,150 mg / kg),and all drugs were administrated to mice by abdominal injection. The activity of superoxide dismutase( SOD), glutathione peroxidase( GSH-Px),catalase( CAT),lactate dehydrogenase( LDH),anti-superoxide anion and the levels of malondialdehvde( MDA) and hydrogen peroxide( H_2O_2) in brain striatum were detected by UV spectrophotometry, and morphologic changes of brain tissue of mice were observed with HE staining.RESULTS: Compared to the model group,treatment of SP in certain concentrations increased the activity of SOD,GSH-Px,CAT,LDH,anti-superoxide anion to varying degree and decreased the levels of H_2O_2 and MDA; intercellular space in the hippocampal region reduced significantly and the neurons became dense and ordered,showing a remarkable recovery from the damage induced by MPTP. CONCLUSION: SP may have a neuroprotective effect on injured neurons induced by MPTP through decreasing oxidative stress reaction.
AIM: To investigate the effects of sulfated pachymaran( SP) on cerebral tissue antioxidant indexes in model mice of Parkinsons ' disease( PD) induced by MPTP. METHODS: ICR mice were treated with MPTP to make PD model. The mice were randomly divided into five groups: control group,MPTP model group and SP group( 50,100,150 mg / kg),and all drugs were administrated to mice by abdominal injection. The activity of superoxide dismutase( SOD), glutathione peroxidase( GSH-Px),catalase( CAT),lactate dehydrogenase( LDH),anti-superoxide anion and the levels of malondialdehvde( MDA) and hydrogen peroxide( H_2O_2) in brain striatum were detected by UV spectrophotometry, and morphologic changes of brain tissue of mice were observed with HE staining.RESULTS: Compared to the model group,treatment of SP in certain concentrations increased the activity of SOD,GSH-Px,CAT,LDH,anti-superoxide anion to varying degree and decreased the levels of H_2O_2 and MDA; intercellular space in the hippocampal region reduced significantly and the neurons became dense and ordered,showing a remarkable recovery from the damage induced by MPTP. CONCLUSION: SP may have a neuroprotective effect on injured neurons induced by MPTP through decreasing oxidative stress reaction.
引文
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[16]李洁欣,李倩,李洁,等.白杨素抗氧化应激对实验性糖尿病大鼠脑损伤的保护作用[J].中国临床药理学与治疗学,2015,20(9):967-970.
[2]Yong-Kee CJ,Warre R,Monnier PP,et al.Evidence for synergism between cell death mechanisms in a cellular model of neurodegeneration in Parkinson's disease[J].Neurotox Res,2012,22(4):355-364.
[3]Jenner P,Olanow CW.Oxidative stress and the pathogenesis of Parkinson's disease[J].Neurology,1996,47(Suppl 3):S161-S170.
[4]王纪凯,王琳,张轶,等.黄芩苷对帕金森病小鼠模型的影响[J].长春中医药大学学报,2015,31(3):454-456.
[5]杨西晓,杨莉,霍启录,等.青玄片对帕金森模型小鼠行为学及纹状体肿瘤坏死因子的影响[J].中药材,2009,32(10):1560-1563.
[6]Salat D,Tolosa E.Levodopa in the treatment of Parkinson's disease:current status and new developments[J].J Parkinsons Dis,2013,3(3):255-269.
[7]李佳青,李文妍,银金兰.治疗帕金森病药物的研究进展[J].中国临床医生,2012,40(12):24-26.
[8]程水明,桂元,沈思,等.茯苓皮三萜类物质抗氧化活性研究[J].食品科学,2011,32(9):27-30.
[9]牛爽,郝利民,赵树欣,等.茯苓多糖的研究进展[J].食品科学,2012,33(13):348-353.
[10]梅天笑,王小莺,万根,等.多糖的修饰及其抗凝血活性研究进展[J].江西农业大学学报,2013,35(5):1108-1113.
[11]高贵珍,陈群,李旭亮.硫酸化茯苓多糖药理性质的研究[J].安徽大学学报,2009,33(6):91-95.
[12]李绪亮,焦庆才,陈群,等.硫酸化茯苓多糖对大鼠慢性肾功能衰竭的防治作用[J].中国药学杂志,2005,40(12):908-911.
[13]高贵珍,焦庆才,李旭亮,等.家兔血浆中硫酸化茯苓多糖的血药浓度的测定[J].中国药学杂志,2005,40(2):134-136.
[14]高贵珍,吴超,陈哲,等.硫酸化茯苓多糖对MPP+诱导的PC12细胞损伤的保护作用研究[J].淮北师范大学学报,2015,36(4):52-58.
[15]高贵珍,王俊博,姜芳,等.硫酸化茯苓多糖对MPTP诱导帕金森小鼠的神经保护作用研究[J].中国药理学通报,2015,31(12):1699-1074.
[16]李洁欣,李倩,李洁,等.白杨素抗氧化应激对实验性糖尿病大鼠脑损伤的保护作用[J].中国临床药理学与治疗学,2015,20(9):967-970.