热水法提取黄皮疣柄牛肝菌多糖工艺研究
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摘要
探讨热水法提取黄皮疣柄牛肝菌多糖,选择液料比、提取温度和提取时间进行单因素试验,应用响应曲面法进行优化,优化后的提取工艺条件为:液料比34∶1(mL/g),提取温度51℃,提取时间3.1 h,多糖的提取率为16.67%,与预测值接近,表明响应面优化的模型参数准确可靠。
The paper was aimed to study the extraction of polysaccharides of Leccinum crocipodium(Letellier.)Watliag by hot water method. Liquid-solid ratio,extraction temperature and time were selected as single factors,and the response surface method was used to optimize the process. The optimum conditions were as follows:liquid-solid ratio 34 ∶ 1(mL/g),the extraction temperature 51 ℃,the extraction time 3.1 h,the extraction rate of the polysaccharide was 16.67 %,which was close to the predicted value. It showed that the model parameters of the response surface optimization were accurate and reliable.
The paper was aimed to study the extraction of polysaccharides of Leccinum crocipodium(Letellier.)Watliag by hot water method. Liquid-solid ratio,extraction temperature and time were selected as single factors,and the response surface method was used to optimize the process. The optimum conditions were as follows:liquid-solid ratio 34 ∶ 1(mL/g),the extraction temperature 51 ℃,the extraction time 3.1 h,the extraction rate of the polysaccharide was 16.67 %,which was close to the predicted value. It showed that the model parameters of the response surface optimization were accurate and reliable.
引文
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[11]冯嫣,马文婷.响应面法优化灵芝孢子粉多糖提取工艺的研究[J].食品研究与开发,2017,38(7):51-55,63
[12]Yan YL,Yu CH,Chen J,et al.Ultrasonic-assisted extraction optimized by response surface methodology,chemical composition and antioxidant activity of polysaccharides from Tremella mesenterica[J].Carbohydr Polym,2011,83:217-224
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[14]徐红霞.野生枝瑚菌多糖的提取、分离纯化和抗氧化性研究[D].杭州:浙江大学,2017:15-19
[15]徐运飞,刘琴,宋珅,等.响应面法优化黄参多糖的提取工艺及其体外抗氧化活性[J].天然产物研究与开发,2015,27(12):2116-2123
[2]Lavi I,Friesem D,Geresh S,et al.An aqueous polysaccharide extract from the edible mushroom Pleurotus ostreatus induces antiproliferative and pro-apoptotic effects on HT-29 colon cancer cells[J].Cancer Letters,2006,244(1):61-70
[3]牛天增,刘娟,王永宏,等.香菇多糖提取技术的研究进展[J].食品研究与开发,2016,37(4):212-216
[4]卯晓岚.中国大型真菌[M].成都:河南科学技术出版社,2000:305-360
[5]黄年来.中国大型真菌原色图鉴[M].中国农业出版社,1998:189-210
[6]梅文泉,董宝生,和丽忠,等.黄皮疣柄牛肝菌营养成分分析[J].中国食用菌,2005,24(1):25-26
[7]刘秋鸣,李笑,肖俊江,等.云南产黄皮疣柄牛肝菌基本营养成分和抗氧化活性[J].食品工业科技,2018,39(16):275-280
[8]Wang H,Jiang H,Wang S,et al.Extraction,purification and preliminary characterization of polysaccharides from Kadsura marmorata fruits[J].Carbohydr Polym,2013,92(2):1901-1907
[9]张岚,和劲松,王雪峰,等.响应面优化复合酶法提取干巴菌多糖工艺[J].食品研究与开发,2017,38(20):51-54,173
[10]Wang JL,Zhang J,Wang XF,et al.A comparison study onmicrowaveassisted extraction of Artemisia sphaerocephala polysaccharides with conventional method:Molecule structure and antioxidant activities evaluation[J].Int J Biol Macromol,2009,45:483-492
[11]冯嫣,马文婷.响应面法优化灵芝孢子粉多糖提取工艺的研究[J].食品研究与开发,2017,38(7):51-55,63
[12]Yan YL,Yu CH,Chen J,et al.Ultrasonic-assisted extraction optimized by response surface methodology,chemical composition and antioxidant activity of polysaccharides from Tremella mesenterica[J].Carbohydr Polym,2011,83:217-224
[13]费荣昌.试验设计与数据处理(第四版)[M].无锡:江南大学出版社,2001:59-63
[14]徐红霞.野生枝瑚菌多糖的提取、分离纯化和抗氧化性研究[D].杭州:浙江大学,2017:15-19
[15]徐运飞,刘琴,宋珅,等.响应面法优化黄参多糖的提取工艺及其体外抗氧化活性[J].天然产物研究与开发,2015,27(12):2116-2123