苹果响应轮纹病菌侵染的分子机制研究进展
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摘要
苹果轮纹病是子囊真菌Botryosphaeria dothidea引起的病害之一,每年造成重大经济损失,全面而深刻理解苹果响应该菌侵染的分子机制有助于采用有效防治措施。综述了苹果轮纹病的病原、侵染循环、抗病机制及防治措施等方面的新进展,侧重阐述与抗(感)病密切相关的基因及蛋白质,为全面理解该病害的抗(感)病机理奠定基础。此外,还讨论了苹果轮纹病菌的生防菌种,为预防性药剂的开发和应用提供依据。
The apple ring rot caused by fungus Botryosphaeria dothidea is a severe disease, which leads to considerable economic loss annually. Comprehensive and profound understanding of the molecular response mechanisms of apple to B.dothidea is crucial for implementing feasible control measures. To this end, this review summarizes the state of the research advances of apple ring rot, covering pathogen, infection cycle, resistance mechanisms, as well as disease control strategies. In particular, this review outlines the genes and proteins closely related to resistance and susceptibility, aiming to portray a clear picture of the mechanisms underlying resistance(susceptibility). Finally, biocontrol strains are also discussed with aiming at providing valuable insights for future applications.
The apple ring rot caused by fungus Botryosphaeria dothidea is a severe disease, which leads to considerable economic loss annually. Comprehensive and profound understanding of the molecular response mechanisms of apple to B.dothidea is crucial for implementing feasible control measures. To this end, this review summarizes the state of the research advances of apple ring rot, covering pathogen, infection cycle, resistance mechanisms, as well as disease control strategies. In particular, this review outlines the genes and proteins closely related to resistance and susceptibility, aiming to portray a clear picture of the mechanisms underlying resistance(susceptibility). Finally, biocontrol strains are also discussed with aiming at providing valuable insights for future applications.
引文
[1]Tang W,Ding Z,Zhou Z Q,et al.Phylogenetic and pathogenic analyses show that the causal agent of apple ring rot in China is Botryosphaeria dothidea.Plant Disease,2012,96(4):486-496.
[2]赵嘉平,梁军,吕全,等.葡萄座腔菌属(Botryosphaeria)系统分类评述.林业科学研究,2007,20(3):415-422.
[3]Slippers B,Wingfield M J.Combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea.Mycologia,2004,96(1):83-101.
[4]Xu C,Wang C S,Sun X Y,et al.Multiple group I introns in the small-subunit r DNA of Botryosphaeria dothidea:implication forintraspecific genetic diversity.Plos One,2013,8(7):e67808.
[5]Marsberg A,Kemler M,Jami F,et al.Botryosphaeria dothidea:a latent pathogen of global importance to woody plant health.Molecular Plant Pathology,2017,18(4):477-488.
[6]陈瑜.苹果轮纹病的发生特点及综合防治措施.现代农业科技,2016(16):118.
[7]Zhai L,Zhang M,Lv G,et al.Biological and molecular characterization of four Botryosphaeria species isolated from pear plants showing stem wart and stem canker in China.Plant Disease,2014,98(6):716-726.
[8]康玲,郝红梅,杨振英,等.苹果轮纹病研究进展.中国农学通报,2009,25(9):188-191.
[9]国立耘,李金云,李保华,等.中国苹果枝干轮纹病发生和防治情况.植物保护,2009,35(4):120-123.
[10]吴贵本,刘传德,王继秋,等.苹果轮纹病和炭疽病病原菌生物学研究.中国果树,2001(1):7-11.
[11]柴全喜,宋素智.梨轮纹病的防治方法.山西果树,2015(4):60.
[12]李广旭,高艳敏,杨华,等.轮纹病菌在苹果枝干上侵入途径的扫描电镜观察.果树学报,2005,22(2):169-171.
[13]Han Q M,Gao X N,Wang J Q,et al.Cytological and histological studies of the interaction between Botryosphaeria dothidea and apple twigs.Scientia Horticulturae,2016,202:142-149.
[14]韩青梅,王婧群,林月莉,等.葡萄座腔菌Botryosphaeria dothidea侵染及其对苹果果实影响的组织细胞学研究.菌物学报,2014,33(1):36-46.
[15]李广旭,杨华,高圣,等.轮纹病菌侵染对不同抗性苹果品种膜透性及防御酶的影响.植物保护学报,2006,33(2):127-130.
[16]丁丽娜,杨瑞英,杨国兴.植物与病原菌互作的蛋白质组学研究进展.微生物学通报,2016,43(2):394-402.
[17]胡海燕.水稻苗/穗瘟防卫反应相关基因的分离与鉴定.北京:中国农业科学院,2006.
[18]Guan Y Q,Chang R F,Liu G J,et al.Role of lenticels and microcracks on susceptibility of apple fruit to Botryosphaeria dothidea.European Journal of Plant Pathology,2015,143(2):317-330.
[19]李淼,檀根甲,承河元,等.植物抗病性研究现状与前景展望.江西农业大学学报(自然科学版),2002,24(5):731-736.
[20]刘海英,李川,范永山,等.影响苹果果实轮纹病抗性的寄主因素及相关性分析.河北农业大学学报,2003,26(1):56-60.
[21]顾雪迎,王洪凯,郭庆元.苹果、梨轮纹病研究进展.浙江农业科学,2015,56(8):1242-1246.
[22]赵晓芳.鸭梨果实抗性物质与轮纹病菌互作关系研究.郑州:河南农业大学,2008.
[23]沈永波,李广旭,高艳敏,等.苹果果实内含物与轮纹病抗性的关系.北方果树,2003(6):9-10.
[24]Holb I J,Balla B,Vamos A,et al.Influence of preharvest calcium applications,fruit injury,and storage atmospheres on postharvest brown rot of apple.Postharvest Biology and Technology,2012,67(7):29-36.
[25]张芮.苹果MdWRKY33基因在轮纹病抗性形成中的作用机制研究.泰安:山东农业大学,2015.
[26]周茜茜,邱化荣,何晓文,等.Md WRKY40介导提高苹果与拟南芥对轮纹病菌的免疫抗性.中国农业科学,2018,51(21):4052-4064.
[27]崔镁沙,庄艳,申飞,等.苹果果实轮纹病抗病性QTL定位及相关基因的初步预测.果树学报,2014,31(5):793-800.
[28]柏素花.苹果轮纹病防御相关基因的鉴定及功能分析.长沙:湖南农业大学,2012.
[29]Dong C H,Zhu J,Zhang Y G,et al.Identification of a xyloglucan-specific endo-(1-4)-beta-d-glucanase inhibitor protein from apple(Malus domestica Borkh.)as a potential defense gene against Botryosphaeria dothidea.Plant Science,2015,231:11-19.
[30]丁安然,孙晓红,侯鸿敏,等.苹果Md ERF113基因克隆及响应轮纹病菌侵染的表达分析.青岛农业大学学报(自然科学版),2018,35(3):161-170.
[31]杨春燕,严海燕.植物囊泡运输与抗病性.中国细胞生物学学报,2014,36(4):546-551.
[32]He X W,Huo Y H,Liu X X,et al.Activation of disease resistance against Botryosphaeria dothidea by downregulating the expression of MdSYP121in apple.Horticulture Research,2018,5(1):24.
[33]Zhang W W,Dong C H,Zhang Y G,et al.An apple cyclic nucleotide-gated ion channel gene highly responsive to Botryosphaeria dothidea infection enhances the susceptibility of Nicotiana benthamiana to bacterial and fungal pathogens.Plant Science,2018,269:94-105.
[34]都贝贝.苹果轮纹病抗性相关mi RNA的筛选.南京:南京农业大学,2013.
[35]Yu X Y,Du B B,Gao Z H,et al.Apple ring rot-responsive putative microRNAs revealed by high-throughput sequencing in Malus×domestica Borkh.Molecular Biology Reports,2014,41(8):5273-5286.
[36]张彩霞,田义,张利义,等.苹果枝条表皮应答轮纹病菌侵染的蛋白质组学分析.植物病理学报,2015,45(3):280-287.
[37]Zhang C X,Tian Y,Zhang L Y,et al.Comparative proteomic analysis of apple branches susceptible and resistant to ring rot disease.European Journal of Plant Pathology,2017,148(2):329-341.
[38]孙天骅,李佳,王涛,等.抗病与感病苹果叶片应答轮纹病菌侵染的蛋白质表达差异分析.园艺学报,2018,45(3):409-420.
[39]Sharma R R,Singh D,Singh R.Biological control of postharvest diseases of fruits and vegetables by microbial antagonists:a review.Biological Control,2009,50(3):205-221.
[40]Shen T,Wang C,Yang H,et al.Identification,solid-state fermentation and biocontrol effects of Streptomyces hygroscopicus B04 on strawberry root rot.Applied Soil Ecology,2016,103:36-43.
[41]Toghueo R M,Eke P,Zabalgogeazcoa I,et al.Biocontrol and growth enhancement potential of two endophytic Trichoderma spp.from Terminalia catappa against the causative agent of common bean root rot(Fusarium solani).Biological Control,2016(6):8-20.
[42]Gao K X,Liu X G,Liu Y H,et al.Potential of Trichoderma harzianum and T.atroviride to control Botryosphaeria berengeriana f.sp.piricola,the cause of apple ring rot.Journal of Phytopathology,2002,150(4-5):271-276.
[43]纪兆林,凌筝,张清霞,等.地衣芽孢杆菌对苹果轮纹病菌和炭疽病菌的抑制及其对贮藏期苹果轮纹病的防治作用.果树学报,2008,25(2):209-214.
[44]Li Y,Han L R,Zhang Y,et al.Biological control of apple ring rot on fruit by Bacillus amyloliquefaciens 9001.Plant Pathology Journal,2013,29(2):168-173.
[45]Chen X Y,Zhang Y Y,Fu X C,et al.Isolation and characterization of Bacillus amyloliquefaciens PG12 for the biological control of apple ring rot.Postharvest Biology and Technology,2016,115:113-121.
[46]Fan H Y,Ru J J,Zhang Y Y,et al.Fengycin produced by Bacillus subtilis9407 plays a major role in the biocontrol of apple ring rot disease.Microbiological Research,2017,199:89-97.
[47]雍道敬,王彩霞,李桂舫,等.内生放线菌A-1对苹果果实轮纹病的防效及防御性酶活性的影响.植物保护学报,2014,41(3):335-341.
[48]Zhang Q M,Yong D J,Zhang Y,et al.Streptomyces rochei A-1 induces resistance and defense-related responses against Botryosphaeria dothidea in apple fruit during storage.Postharvest Biology and Technology,2016,115:30-37.
[49]黄玲玲,裘纪莹,唐琳,等.解淀粉芽胞杆菌NCPSJ7对采后苹果轮纹病的生物防治作用.中国食物与营养,2015,21(2):20-24.
[50]李铁军,丁邦琴,蔡凤,等.生防菌菌株LDB1对植物病原真菌的抑菌谱.湖北农业科学,2015,54(2):341-345.
[51]赵云福,邱东晓,梁晨,等.苹果病原真菌拮抗菌HMQAU140045的分离与鉴定.山东农业科学,2017,49(12):54-58.
[52]Liu Y Z,Chen Z Y,Liu Y F,et al.Enhancing bioefficacy of Bacillus subtilis with sodium bicarbonate for the control of ring rot in pear during storage.Biological Control,2011,57(2):110-117.
[2]赵嘉平,梁军,吕全,等.葡萄座腔菌属(Botryosphaeria)系统分类评述.林业科学研究,2007,20(3):415-422.
[3]Slippers B,Wingfield M J.Combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea.Mycologia,2004,96(1):83-101.
[4]Xu C,Wang C S,Sun X Y,et al.Multiple group I introns in the small-subunit r DNA of Botryosphaeria dothidea:implication forintraspecific genetic diversity.Plos One,2013,8(7):e67808.
[5]Marsberg A,Kemler M,Jami F,et al.Botryosphaeria dothidea:a latent pathogen of global importance to woody plant health.Molecular Plant Pathology,2017,18(4):477-488.
[6]陈瑜.苹果轮纹病的发生特点及综合防治措施.现代农业科技,2016(16):118.
[7]Zhai L,Zhang M,Lv G,et al.Biological and molecular characterization of four Botryosphaeria species isolated from pear plants showing stem wart and stem canker in China.Plant Disease,2014,98(6):716-726.
[8]康玲,郝红梅,杨振英,等.苹果轮纹病研究进展.中国农学通报,2009,25(9):188-191.
[9]国立耘,李金云,李保华,等.中国苹果枝干轮纹病发生和防治情况.植物保护,2009,35(4):120-123.
[10]吴贵本,刘传德,王继秋,等.苹果轮纹病和炭疽病病原菌生物学研究.中国果树,2001(1):7-11.
[11]柴全喜,宋素智.梨轮纹病的防治方法.山西果树,2015(4):60.
[12]李广旭,高艳敏,杨华,等.轮纹病菌在苹果枝干上侵入途径的扫描电镜观察.果树学报,2005,22(2):169-171.
[13]Han Q M,Gao X N,Wang J Q,et al.Cytological and histological studies of the interaction between Botryosphaeria dothidea and apple twigs.Scientia Horticulturae,2016,202:142-149.
[14]韩青梅,王婧群,林月莉,等.葡萄座腔菌Botryosphaeria dothidea侵染及其对苹果果实影响的组织细胞学研究.菌物学报,2014,33(1):36-46.
[15]李广旭,杨华,高圣,等.轮纹病菌侵染对不同抗性苹果品种膜透性及防御酶的影响.植物保护学报,2006,33(2):127-130.
[16]丁丽娜,杨瑞英,杨国兴.植物与病原菌互作的蛋白质组学研究进展.微生物学通报,2016,43(2):394-402.
[17]胡海燕.水稻苗/穗瘟防卫反应相关基因的分离与鉴定.北京:中国农业科学院,2006.
[18]Guan Y Q,Chang R F,Liu G J,et al.Role of lenticels and microcracks on susceptibility of apple fruit to Botryosphaeria dothidea.European Journal of Plant Pathology,2015,143(2):317-330.
[19]李淼,檀根甲,承河元,等.植物抗病性研究现状与前景展望.江西农业大学学报(自然科学版),2002,24(5):731-736.
[20]刘海英,李川,范永山,等.影响苹果果实轮纹病抗性的寄主因素及相关性分析.河北农业大学学报,2003,26(1):56-60.
[21]顾雪迎,王洪凯,郭庆元.苹果、梨轮纹病研究进展.浙江农业科学,2015,56(8):1242-1246.
[22]赵晓芳.鸭梨果实抗性物质与轮纹病菌互作关系研究.郑州:河南农业大学,2008.
[23]沈永波,李广旭,高艳敏,等.苹果果实内含物与轮纹病抗性的关系.北方果树,2003(6):9-10.
[24]Holb I J,Balla B,Vamos A,et al.Influence of preharvest calcium applications,fruit injury,and storage atmospheres on postharvest brown rot of apple.Postharvest Biology and Technology,2012,67(7):29-36.
[25]张芮.苹果MdWRKY33基因在轮纹病抗性形成中的作用机制研究.泰安:山东农业大学,2015.
[26]周茜茜,邱化荣,何晓文,等.Md WRKY40介导提高苹果与拟南芥对轮纹病菌的免疫抗性.中国农业科学,2018,51(21):4052-4064.
[27]崔镁沙,庄艳,申飞,等.苹果果实轮纹病抗病性QTL定位及相关基因的初步预测.果树学报,2014,31(5):793-800.
[28]柏素花.苹果轮纹病防御相关基因的鉴定及功能分析.长沙:湖南农业大学,2012.
[29]Dong C H,Zhu J,Zhang Y G,et al.Identification of a xyloglucan-specific endo-(1-4)-beta-d-glucanase inhibitor protein from apple(Malus domestica Borkh.)as a potential defense gene against Botryosphaeria dothidea.Plant Science,2015,231:11-19.
[30]丁安然,孙晓红,侯鸿敏,等.苹果Md ERF113基因克隆及响应轮纹病菌侵染的表达分析.青岛农业大学学报(自然科学版),2018,35(3):161-170.
[31]杨春燕,严海燕.植物囊泡运输与抗病性.中国细胞生物学学报,2014,36(4):546-551.
[32]He X W,Huo Y H,Liu X X,et al.Activation of disease resistance against Botryosphaeria dothidea by downregulating the expression of MdSYP121in apple.Horticulture Research,2018,5(1):24.
[33]Zhang W W,Dong C H,Zhang Y G,et al.An apple cyclic nucleotide-gated ion channel gene highly responsive to Botryosphaeria dothidea infection enhances the susceptibility of Nicotiana benthamiana to bacterial and fungal pathogens.Plant Science,2018,269:94-105.
[34]都贝贝.苹果轮纹病抗性相关mi RNA的筛选.南京:南京农业大学,2013.
[35]Yu X Y,Du B B,Gao Z H,et al.Apple ring rot-responsive putative microRNAs revealed by high-throughput sequencing in Malus×domestica Borkh.Molecular Biology Reports,2014,41(8):5273-5286.
[36]张彩霞,田义,张利义,等.苹果枝条表皮应答轮纹病菌侵染的蛋白质组学分析.植物病理学报,2015,45(3):280-287.
[37]Zhang C X,Tian Y,Zhang L Y,et al.Comparative proteomic analysis of apple branches susceptible and resistant to ring rot disease.European Journal of Plant Pathology,2017,148(2):329-341.
[38]孙天骅,李佳,王涛,等.抗病与感病苹果叶片应答轮纹病菌侵染的蛋白质表达差异分析.园艺学报,2018,45(3):409-420.
[39]Sharma R R,Singh D,Singh R.Biological control of postharvest diseases of fruits and vegetables by microbial antagonists:a review.Biological Control,2009,50(3):205-221.
[40]Shen T,Wang C,Yang H,et al.Identification,solid-state fermentation and biocontrol effects of Streptomyces hygroscopicus B04 on strawberry root rot.Applied Soil Ecology,2016,103:36-43.
[41]Toghueo R M,Eke P,Zabalgogeazcoa I,et al.Biocontrol and growth enhancement potential of two endophytic Trichoderma spp.from Terminalia catappa against the causative agent of common bean root rot(Fusarium solani).Biological Control,2016(6):8-20.
[42]Gao K X,Liu X G,Liu Y H,et al.Potential of Trichoderma harzianum and T.atroviride to control Botryosphaeria berengeriana f.sp.piricola,the cause of apple ring rot.Journal of Phytopathology,2002,150(4-5):271-276.
[43]纪兆林,凌筝,张清霞,等.地衣芽孢杆菌对苹果轮纹病菌和炭疽病菌的抑制及其对贮藏期苹果轮纹病的防治作用.果树学报,2008,25(2):209-214.
[44]Li Y,Han L R,Zhang Y,et al.Biological control of apple ring rot on fruit by Bacillus amyloliquefaciens 9001.Plant Pathology Journal,2013,29(2):168-173.
[45]Chen X Y,Zhang Y Y,Fu X C,et al.Isolation and characterization of Bacillus amyloliquefaciens PG12 for the biological control of apple ring rot.Postharvest Biology and Technology,2016,115:113-121.
[46]Fan H Y,Ru J J,Zhang Y Y,et al.Fengycin produced by Bacillus subtilis9407 plays a major role in the biocontrol of apple ring rot disease.Microbiological Research,2017,199:89-97.
[47]雍道敬,王彩霞,李桂舫,等.内生放线菌A-1对苹果果实轮纹病的防效及防御性酶活性的影响.植物保护学报,2014,41(3):335-341.
[48]Zhang Q M,Yong D J,Zhang Y,et al.Streptomyces rochei A-1 induces resistance and defense-related responses against Botryosphaeria dothidea in apple fruit during storage.Postharvest Biology and Technology,2016,115:30-37.
[49]黄玲玲,裘纪莹,唐琳,等.解淀粉芽胞杆菌NCPSJ7对采后苹果轮纹病的生物防治作用.中国食物与营养,2015,21(2):20-24.
[50]李铁军,丁邦琴,蔡凤,等.生防菌菌株LDB1对植物病原真菌的抑菌谱.湖北农业科学,2015,54(2):341-345.
[51]赵云福,邱东晓,梁晨,等.苹果病原真菌拮抗菌HMQAU140045的分离与鉴定.山东农业科学,2017,49(12):54-58.
[52]Liu Y Z,Chen Z Y,Liu Y F,et al.Enhancing bioefficacy of Bacillus subtilis with sodium bicarbonate for the control of ring rot in pear during storage.Biological Control,2011,57(2):110-117.