复方鳖甲软肝片防治大鼠肝纤维化作用及机制
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摘要
目的:探讨复方鳖甲软肝片对大鼠肝纤维化的干预作用及机制。方法:SD成年大鼠随机分为空白对照组(A组)、复方鳖甲软肝片治疗组(B组)、复方鳖甲软肝片干预组(C组)和模型对照组(D组),B、C、D组大鼠采用CCl4诱导大鼠肝纤维化模型,B组于造模第6周给予复方鳖甲软肝片,C组在造模的同时给予复方鳖甲软肝片;于造模第6、12周检测大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、白蛋白(ALB)、总胆红素(TBIL)等肝功能指标,造模第12周处死大鼠检测肝脏指数,ELISA检测大鼠血清透明质酸、层黏连蛋白、Ⅲ型前胶原、Ⅳ型胶原等肝纤维化指标;提取各组大鼠肝脏Kupffer细胞,免疫荧光染色检测CD14表达,观察Kupffer细胞活化情况;实时荧光定量PCR及Western Blot分别检测Kupffer细胞中TGF-βmRNA及蛋白表达水平。结果:经复方鳖甲软肝片干预或治疗后,B、C组大鼠肝脏指数、肝纤维化指标、肝功能指标均优于D组,差异具有统计学意义(P <0. 05); D组Kupffer细胞中CD14呈阳性,B、C组CD14呈阴性; B、C组大鼠kupffer细胞中TGF-β蛋白及mRNA水平显著低于D组,差异具有统计学意义(P <0. 05)。结论:复方鳖甲软肝片具有抑制肝Kupffer细胞活化的作用,并可能通过抑制其分泌TGF-β而发挥抗肝纤维化的作用。
Objective: To investigate the preventive and therapeutic effects of Fufang Biejia Ruangan Tablet on CCl4-induced hepatic fibrosis in rats and its action mode. Methods: Sprague-Dawley( SD)adult rats were randomly divided into control group( group A),group B,group C and group D. Rats in groups B,C and D were treated with CCl4 to induce rat liver fibrosis model. Rats in group C were given with Fufang Biejia Ruangan Tablets at the same time as ClC4 treatment. Rats in group B were given with Fufang Biejia Ruangan Tablets at 6th week after ClC4 treatment. At the 6th and 12 th week of ClC4 treatment,serum alanine aminotransferase( ALT),aspartate aminotransferase( AST),albumin( ALB) and total bilirubin( TBIL) were measured and the rats were sacrificed at the 12 th week.The liver fibrosis indexes such as serum hyaluronic acid,laminin,type III procollagen and type IV collagen were detected by ELISA. CD14 expression was detected by immunofluorescent staining in Kupffer cells isolated from the livers of each group to observe Kupffer cell activation; transforming growth factor( TGF-β) expression in Kupffer cells and livers was assessed by real-time quantitative PCR and Western Blot. Results: After treatment with Fufang Biejia Ruangan Tablets,the health status including the liver index,liver fibrosis index and liver function index of rats in group B and C was better than that of rats in group D,the difference was statistically significant( P < 0. 05); CD14 expression was positive in Kupffer cells in group D,but negative in group A,B and C. Moreover,the levels of TGF-β at both mRNA protein in kupffer cells in group B and C were significantly lower than those in group D( P < 0. 05). Taken together,anti-fibrosis effect of Fufang Biejia Ruangan Tablet might impairs TGF-β expression and secretion by Kupffer cells via inhibiting the activation of Kupffer cells.
Objective: To investigate the preventive and therapeutic effects of Fufang Biejia Ruangan Tablet on CCl4-induced hepatic fibrosis in rats and its action mode. Methods: Sprague-Dawley( SD)adult rats were randomly divided into control group( group A),group B,group C and group D. Rats in groups B,C and D were treated with CCl4 to induce rat liver fibrosis model. Rats in group C were given with Fufang Biejia Ruangan Tablets at the same time as ClC4 treatment. Rats in group B were given with Fufang Biejia Ruangan Tablets at 6th week after ClC4 treatment. At the 6th and 12 th week of ClC4 treatment,serum alanine aminotransferase( ALT),aspartate aminotransferase( AST),albumin( ALB) and total bilirubin( TBIL) were measured and the rats were sacrificed at the 12 th week.The liver fibrosis indexes such as serum hyaluronic acid,laminin,type III procollagen and type IV collagen were detected by ELISA. CD14 expression was detected by immunofluorescent staining in Kupffer cells isolated from the livers of each group to observe Kupffer cell activation; transforming growth factor( TGF-β) expression in Kupffer cells and livers was assessed by real-time quantitative PCR and Western Blot. Results: After treatment with Fufang Biejia Ruangan Tablets,the health status including the liver index,liver fibrosis index and liver function index of rats in group B and C was better than that of rats in group D,the difference was statistically significant( P < 0. 05); CD14 expression was positive in Kupffer cells in group D,but negative in group A,B and C. Moreover,the levels of TGF-β at both mRNA protein in kupffer cells in group B and C were significantly lower than those in group D( P < 0. 05). Taken together,anti-fibrosis effect of Fufang Biejia Ruangan Tablet might impairs TGF-β expression and secretion by Kupffer cells via inhibiting the activation of Kupffer cells.
引文
[1]马龙俊. Kupffer细胞与肝纤维化关系的研究进展[J].宜春学院学报,2015,37(3):85-87.
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[5]李洋,蔡双明,张莉莉,等.大鼠原代肝细胞、星状细胞、枯否细胞和肝窦内皮细胞的同步分离与培养[J].南方医科大学学报,2014,34(4):532-537.
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[9]黄艳,黄成,李俊.肝纤维化病程中Kupffer细胞分泌的细胞因子对肝星状细胞活化增殖、凋亡的调控[J].中国药理学通报,2010,26(1):9-13.
[10]谷莉莉,李海. Kupffer细胞在肝纤维化发生过程中的双向调节作用[J].临床肝胆病杂志,2012,28(9):714-716.
[11]熊钻,梅玉峰,王春阳,等.透明质酸、Ⅲ型前胶原、Ⅳ型胶原、层黏连蛋白对肝硬化的诊断价值分析[J].中国处方药,2018(1):137-138.
[12]姚丹燕.透明质酸、Ⅲ型前胶原、Ⅳ型胶原、层黏连蛋白和脯氨酸肽酶对肝硬化的诊断价值分析[J].中国保健营养,2017,27(4):14-16.
[13]肖蕾,王云莲,艾力江·吐尔逊,等.血清透明质酸、Ⅳ型胶原、层粘连蛋白与大鼠放射性肝纤维化的相关性研究[J].中华全科医学,2015,13(1):18-20.
[14]刘建国,丁艳蕊,杨胜兰,等.丹酚酸B对实验性肝纤维化大鼠肝组织CD14表达的影响[J].中国中西医结合杂志,2011,31(4):547-551.
[15]常永丽,巩继平,郭晓姝,等.异丙酚对严重烫伤早期大鼠肝NF-κΒ、CD14表达及凋亡的影响[J].长治医学院学报,2018,32(3):161-164.
[2] WEISKIRCHEN R F. Tacke,cellular and molecular functions of hepatic stellate cells in inflammatory responses and liver immunology[J]. Hepatobiliary Surg Nutr,2014,3(6):344-363.
[3]WANG J,LECLERCQ I,BRYMORA J M,et al. Kupffer cells mediate leptin-induced hepatic fibrosis[J]. Gastroenterology,2009,137(2):713-723.
[4]黄灵跃,施维群,孟庆宇.复方鳖甲软肝片抗肝纤维化的现状及展望[J].江西中医药大学学报,2015,(4):97-99.
[5]李洋,蔡双明,张莉莉,等.大鼠原代肝细胞、星状细胞、枯否细胞和肝窦内皮细胞的同步分离与培养[J].南方医科大学学报,2014,34(4):532-537.
[6]张玉姣.肝星状细胞和枯否细胞在肝纤维化过程中的相互作用[D].长春:吉林大学,2015.
[7]WALTON K L,JOHNSON K E,HARRISON C A. Targeting TGF-βmediated SMAD signaling for the prevention of fibrosis[J]. Frontiers in Pharmacology, 2017, 8(461):1-8
[8]ZHOU L,DONG X,WANG L,et al. Casticin attenuates liver fibrosis and hepatic stellate cell activation by blocking TGF-β/Smad signaling pathway.[J]. Oncotarget,2017,8(34):56267-56280.
[9]黄艳,黄成,李俊.肝纤维化病程中Kupffer细胞分泌的细胞因子对肝星状细胞活化增殖、凋亡的调控[J].中国药理学通报,2010,26(1):9-13.
[10]谷莉莉,李海. Kupffer细胞在肝纤维化发生过程中的双向调节作用[J].临床肝胆病杂志,2012,28(9):714-716.
[11]熊钻,梅玉峰,王春阳,等.透明质酸、Ⅲ型前胶原、Ⅳ型胶原、层黏连蛋白对肝硬化的诊断价值分析[J].中国处方药,2018(1):137-138.
[12]姚丹燕.透明质酸、Ⅲ型前胶原、Ⅳ型胶原、层黏连蛋白和脯氨酸肽酶对肝硬化的诊断价值分析[J].中国保健营养,2017,27(4):14-16.
[13]肖蕾,王云莲,艾力江·吐尔逊,等.血清透明质酸、Ⅳ型胶原、层粘连蛋白与大鼠放射性肝纤维化的相关性研究[J].中华全科医学,2015,13(1):18-20.
[14]刘建国,丁艳蕊,杨胜兰,等.丹酚酸B对实验性肝纤维化大鼠肝组织CD14表达的影响[J].中国中西医结合杂志,2011,31(4):547-551.
[15]常永丽,巩继平,郭晓姝,等.异丙酚对严重烫伤早期大鼠肝NF-κΒ、CD14表达及凋亡的影响[J].长治医学院学报,2018,32(3):161-164.