木槿花多糖的组成分析及抗氧化活性研究
|
摘要
木槿花具有较好的观赏及食药用价值,该试验选取粉花重瓣及白花重瓣2个品种的木槿花4个样品进行多糖最佳提取条件、相对分子质量、单糖组成及抗氧化活性分析,以期为木槿花的应用提供理论基础。通过单因素试验和响应面设计优化木槿花多糖的最佳提取条件:提取温度96.8℃,液料比43.5∶1(m L·g~(-1)),提取时间3.1 h。利用高效凝胶色谱分析发现4种木槿花多糖均由单一峰组成,其平均相对分子质量分别为1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5;采用柱前衍生HPLC分析发现木槿花多糖主要由葡萄糖、甘露糖、半乳糖、鼠李糖和阿拉伯糖5种单糖组成,其中半乳糖组成比例最高,其与葡萄糖的比值为1.656～4.496。通过DPPH自由基、Fe~(3+)还原能力和ABTS~(+·)自由基等抗氧化活性测定,木槿花粗多糖均表现出潜在的抗氧化活性,且其抗氧化活性与粗多糖浓度呈现良好的量效关系。通过以上分析,粉花重瓣与白花重瓣的木槿花在多糖组成上一致,其相对分子质量及单糖组成差异不大,但粉花重瓣的抗氧化活性优于白花重瓣,该研究结果为木槿花的食用功能提供了理论基础。
The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.
The flower of Hibiscus syriacus has good ornamental and edible-medicinal values.In this study,four samples of two varieties,namely white multiple petals flower and pink multiple petals flower,were selected as test materials.And the optimum extraction conditions,relative molecular weight,monosaccharide composition and antioxidant activity of polysaccharides in flower were investigated.Through single factor experiment and response surface,the optimal extract conditions of polysaccharide were designed as follows:extraction temperature at 96.8℃,ratio of material to liquid of 43.5∶1 m L·g~(-1),extraction time of 3.1 h.Polysaccharides of H.syriacus flowers were analyzed by high performance gel chromatography.The average molecular masses of the 4 polysaccharide samples were1.49×10~5,1.25×10~5,1.01×10~5,1.37×10~5,respectively.Polysaccharides of H.syriacus flowers were mainly composed of glucose,mannose,galactose,rhamnose and arabinose by pre-column derivatization HPLC.The ratio of galactose was the highest in five monosaccharide,and the ratio of galactose to glucose was 1.656-4.496.In addition,crude polysaccharides of H.syriacus flowers showed potential antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl radical(DPPH)assay,total reducing capacity assay and ABTS assay in vitro,and its antioxidant effect showed a good dose-effect relationship with the concentration of crude polysaccharides.Among the tested varieties,polysaccharides of pink multiple petals flower and white multiple petals flower had the same molecular masses and monosaccharides composition,but the antioxidant activity of the polysaccharides of pink multiple petals flower was higher than that of the white flowers.The results of this study can provide a theoretical basis for the application of H.syriacus flower in the field of functional foods.
引文
[1]李秀芬,朱建军,张德顺.木槿属树种应用与研究现状分析[J].上海农业学报,2006,22(2):108.
[2]杨涛,王博诚,邓碧珊,等.木槿提取工艺及抗氧化作用研究[J].山东化工,2017,46(12):24.
[3]张文彦,王晓红,李安平,等.木槿功能性营养成分与生物活性研究进展[J].食品与机械,2017,33(2):216.
[4]金月亭,应铁进.木槿花生物活性的初步研究[J].中国食品学报,2008,8(3):37.
[5]景立新,林柏全,战伟,等.木槿花中营养成分分析[J].中国卫生检验杂志,2008,18(9):1871.
[6]Kim J H. Anthocyanin pigmentation patternings in petals of Hibiscus syriacus[J]. J Fac Agric Kyushuu,1989,33(2):253.
[7]Yoo K O,Lim H T,Kim J H. Studies on the flavonoids of the Hibiscus syriacus L. complex[J]. Korean J Plant Res,1996,9(3):224.
[8]Tomoda M,Ichikawa M. Plant mucilages. XL. A representative mucilage,"Hibiscus-mucilage SF",from the flower buds of Hibiscus syriacus[J]. Chem Pharm Bull,1987,35(6):2360.
[9]徐璐,汪涛,郭巧生,等.响应面法优化超声辅助法提取昆仑雪菊色素的工艺研究[J].中国中药杂志,2014,39(24):4792.
[10]张四杰,钱正,刘京晶,等.铁皮石斛花多糖相对分子质量及其单糖组成的研究[J].中国中药杂志,2017,42(20):3919.
[11]杨秀艳,薛志远,杨亚飞,等.红芪多糖的复合酶联合超声提取工艺、理化特性及抗氧化活性的研究[J].中国中药杂志,2018,43(11):2261.
[12]李红法,郭松波,满淑丽,等.乙醇分级沉淀提取黄芪多糖及其理化性质和抗氧化活性研究[J].中国中药杂志,2015,40(11):2112.
[13]徐伟,凌伟坚,褚克丹,等.响应面法优化车前子油的提取工艺及抗氧化活性研究[J].中国中药杂志,2011,36(23):3253.
[14]Jing C,Yuan Y,Tang Q,et al. Extraction optimization preliminary characterization and antioxidant activity of polysaccharides from Glycine soja[J]. Biol Macromol,2017,103(2017):1207.
[15]Xu Z,Feng S,Shen S,et al. The antioxidant activities effect of neutral and acidic polysaccharides from Epimedium acuminatum Franch. on Caenorhabditis elegans[J]. Carbohydr Polym,2016,144(2016):122.
[16]祁小妮,马晓红,李振亮,等.响应曲面优化丹参多糖提取工艺及抗氧化活性分析[J].南方农业学报,2016,47(11):1926.
[17]Zhang Z,Wang F,Wang M,et al. A comparative study of the neutral and acidic polysaccharides from Allium macrostemon Bunge[J]. Carbohyd Polym,2015,117(117):980.
[18]Terpinc P,Cigic'B,Polak T,et al. LC-MS analysis of phenolic compounds and antioxidant activity of buckwheat at different stages of malting[J]. Food Chem,2016,210(2016):9.
[19]Zheng Q,Ren D,Yang N,et al. Optimization for ultrasound-assisted extraction of polysaccharides with chemical composition and antioxidant activity from the Artemisia sphaerocephala Krasch seeds[J]. Int J Biol Macromol,2016,91(2016):856.
[20]吴金霞,贾红梅,于猛,等.柴胡疏肝散体外抗氧化活性成分与调控抑郁症相关酶的关联性分析[J].中国实验方剂学杂志,2018,24(12):74.
[2]杨涛,王博诚,邓碧珊,等.木槿提取工艺及抗氧化作用研究[J].山东化工,2017,46(12):24.
[3]张文彦,王晓红,李安平,等.木槿功能性营养成分与生物活性研究进展[J].食品与机械,2017,33(2):216.
[4]金月亭,应铁进.木槿花生物活性的初步研究[J].中国食品学报,2008,8(3):37.
[5]景立新,林柏全,战伟,等.木槿花中营养成分分析[J].中国卫生检验杂志,2008,18(9):1871.
[6]Kim J H. Anthocyanin pigmentation patternings in petals of Hibiscus syriacus[J]. J Fac Agric Kyushuu,1989,33(2):253.
[7]Yoo K O,Lim H T,Kim J H. Studies on the flavonoids of the Hibiscus syriacus L. complex[J]. Korean J Plant Res,1996,9(3):224.
[8]Tomoda M,Ichikawa M. Plant mucilages. XL. A representative mucilage,"Hibiscus-mucilage SF",from the flower buds of Hibiscus syriacus[J]. Chem Pharm Bull,1987,35(6):2360.
[9]徐璐,汪涛,郭巧生,等.响应面法优化超声辅助法提取昆仑雪菊色素的工艺研究[J].中国中药杂志,2014,39(24):4792.
[10]张四杰,钱正,刘京晶,等.铁皮石斛花多糖相对分子质量及其单糖组成的研究[J].中国中药杂志,2017,42(20):3919.
[11]杨秀艳,薛志远,杨亚飞,等.红芪多糖的复合酶联合超声提取工艺、理化特性及抗氧化活性的研究[J].中国中药杂志,2018,43(11):2261.
[12]李红法,郭松波,满淑丽,等.乙醇分级沉淀提取黄芪多糖及其理化性质和抗氧化活性研究[J].中国中药杂志,2015,40(11):2112.
[13]徐伟,凌伟坚,褚克丹,等.响应面法优化车前子油的提取工艺及抗氧化活性研究[J].中国中药杂志,2011,36(23):3253.
[14]Jing C,Yuan Y,Tang Q,et al. Extraction optimization preliminary characterization and antioxidant activity of polysaccharides from Glycine soja[J]. Biol Macromol,2017,103(2017):1207.
[15]Xu Z,Feng S,Shen S,et al. The antioxidant activities effect of neutral and acidic polysaccharides from Epimedium acuminatum Franch. on Caenorhabditis elegans[J]. Carbohydr Polym,2016,144(2016):122.
[16]祁小妮,马晓红,李振亮,等.响应曲面优化丹参多糖提取工艺及抗氧化活性分析[J].南方农业学报,2016,47(11):1926.
[17]Zhang Z,Wang F,Wang M,et al. A comparative study of the neutral and acidic polysaccharides from Allium macrostemon Bunge[J]. Carbohyd Polym,2015,117(117):980.
[18]Terpinc P,Cigic'B,Polak T,et al. LC-MS analysis of phenolic compounds and antioxidant activity of buckwheat at different stages of malting[J]. Food Chem,2016,210(2016):9.
[19]Zheng Q,Ren D,Yang N,et al. Optimization for ultrasound-assisted extraction of polysaccharides with chemical composition and antioxidant activity from the Artemisia sphaerocephala Krasch seeds[J]. Int J Biol Macromol,2016,91(2016):856.
[20]吴金霞,贾红梅,于猛,等.柴胡疏肝散体外抗氧化活性成分与调控抑郁症相关酶的关联性分析[J].中国实验方剂学杂志,2018,24(12):74.