蒙药查干-珠勒根-其木格的质量标准研究
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摘要
目的建立蒙药材查干-珠勒根-其木格的质量标准,为查干-珠勒根-其木格药用植物资源的开发利用及临床合理应用提供科学依据。方法采集青海、甘肃和西藏三个不同产区共7个批次的查干-珠勒根-其木格的药材样品,进行其性状、显微鉴别;测定其水分、灰分和酸不溶灰分等检查项;采用高效液相色谱测定法测定其所含专属性黄酮类指标成分异荭草素的含量。结果药材粉末的性状和鉴别特征明显;异荭草素含量不得少于0. 10%。结论本项目中建立的质量标准可以应用于查干-珠勒根-其木格药材的质量控制中。
Objective To establish the quality standard based on Gentiana purdomii Marq. and provide scientific basis for the development and utilization of medicinal plant resources of Gentiana purdomii Marq.. Methods Seven batches of medicinal herbs were collected from three different regions of Qinghai,Gansu and Xizang. These were identified by character,microscopic,moisture,ash and acid insoluble ash; Homoorientin is one of the special compounds in Gentiana purdomii Marq.. The content of Homoorientin was determined by HPLC. Results The character of flower and it's distinguishing feature were obvious; The content of Homoorientin shall not be less than 0. 10%. Conclusion The quality standard was established in this project can be used for the quality control of Gentiana purdomii Marq..
Objective To establish the quality standard based on Gentiana purdomii Marq. and provide scientific basis for the development and utilization of medicinal plant resources of Gentiana purdomii Marq.. Methods Seven batches of medicinal herbs were collected from three different regions of Qinghai,Gansu and Xizang. These were identified by character,microscopic,moisture,ash and acid insoluble ash; Homoorientin is one of the special compounds in Gentiana purdomii Marq.. The content of Homoorientin was determined by HPLC. Results The character of flower and it's distinguishing feature were obvious; The content of Homoorientin shall not be less than 0. 10%. Conclusion The quality standard was established in this project can be used for the quality control of Gentiana purdomii Marq..
引文
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[12]黄继荣,海银梅,包文玲,等.蒙药材白花龙胆和尖叶假龙胆的薄层色谱鉴别[J].内蒙古民族大学学报(自然版),2011,26(1):71-72.
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[15]YUAN L,WANG J,WU W Q,et al.Effect of isoorientin on intracellular antioxidant defence mechanisms in hepatoma and liver cell lines[J].Biomedicine&pharmacotherapy,81:356-362.
[16]中华人民共和国药典[M].一部,四部.北京:中国医药卫生出版社,2015:6.
[2]占布拉道尔吉.无误蒙药鉴[M].呼和浩特:内蒙古人民出版社,1988:4.
[3]伊希巴拉珠尔.认药白晶鉴[M].呼和浩特:内蒙古人民出版社,1998:2.
[4]内蒙古自治区医院革命委员会.蒙药验方[M].呼和浩特:内蒙古自治区医院革命委员会出版,1971:7.
[5]罗布桑却因丕勒.哲对宁诺尔[M].呼和浩特:内蒙古人民出版社,1974:7.
[6]伊希巴拉珠尔.甘露四部[M].呼和浩特:内蒙古人民出版社,1998:9.
[7]白雪,曾擎屹,朱奎德,等.CZE法测定白花龙胆6中活性成分含量研究[J].广西师范大学学报志,2016,34(1):122-127.
[8]杨芳,童丽,热增才旦,等.HPLC法测定藏药白花龙胆中4中成分的含量[J].中药材,2015,38(7):1439-440.
[9]宗留留,骆桂法,吴立宏,等.藏药白花龙胆质量标准研究[J].中国中药杂志,2015,40(10):1873-1876.
[10]刘圆,孟庆艳,彭镰心,等.RP-HPLC法测定藏药龙胆花的两种原植物白花龙胆和蓝玉簪龙胆中龙胆苦苷[J].中草药,2006,37(11):1738-1739.
[11]钟世红,古锐,马羚,等.藏药白花龙胆品种考证与使用现状调查[J].中国中药杂志,2014,39(13):2450-2454.
[12]黄继荣,海银梅,包文玲,等.蒙药材白花龙胆和尖叶假龙胆的薄层色谱鉴别[J].内蒙古民族大学学报(自然版),2011,26(1):71-72.
[13]罗成,李艳,龙建刚,等.异荭草素的药理作用[J].航天医学与医学工程,2016,5:381-384.
[14]SHI J,ZHU L J,LI Y,et al.In vitro study of UGT metabolism and permeability of orientin and isoorientin,two active flavonoid C-glycosides[J].Drug Metabolism Letters,10(2):101-110.
[15]YUAN L,WANG J,WU W Q,et al.Effect of isoorientin on intracellular antioxidant defence mechanisms in hepatoma and liver cell lines[J].Biomedicine&pharmacotherapy,81:356-362.
[16]中华人民共和国药典[M].一部,四部.北京:中国医药卫生出版社,2015:6.