不同植入扭矩的牙种植体-骨结合界面的组织学研究
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摘要
目的:以不同的扭矩植入牙种植体,对种植体-骨结合界面进行组织学研究。方法:4只beagle犬的下颌骨分别植入8枚BLBⅢ牙种植体,进行植入扭矩≤15N·cm(A组)、25N·cm(B组)、35N·cm(C组)及≥45N·cm(D组)四组实验。32枚种植体随机分为Ⅰ、Ⅱ两组。两个月后处死动物,标本进行HE染色、亚甲基蓝-酸性品红染色。镜下观察种植体-骨结合界面的反应。结果:32枚种植体均无松动脱落,Ⅰ组新骨较Ⅱ组明显增多。B、C组界面见大量成骨细胞,部分区域出现骨基质。结论:相较于植入扭矩≤15N·cm(A组)、≥45N·cm(D组),植入扭矩25N·cm(B组)、35N·cm(C组)更利于BLBⅢ牙种植体-骨结合。
Objective: To insert dental implants with different insertion torque and analyze the influences of osseointegration between dental implants and surrounding bones. Methods: Select 4 healthy adult beagle dogs as experimental subjects, each dog will insert eight implants. In accordance with the Latin square experimental design methods, we can determine the insertion torque of each site: ≤15N·cm(group A), 25N·cm(group B), 35N·cm(group C)and≥45N·cm(group D). 32 implants were randomly divided into two groups:andⅡ. After 2 months, 4 dogs were sacrificed. The samples undergone HE staining as well as methylene blue acid fuchsin staining and then analyzed by optical microscope observation. Results: All implants were success. New bones growed more actively in groupⅠthan in group Ⅱ.A lot of osteoblast as well as bone matrix were found in group B and group C. Conclusions: For BLBⅢ, compared to insertion torque≤15N·cm(group A) and≥45N·cm(group D), implants with insertion torque of 25N·cm(group B)and 35N·cm(group C)have better osseointegration.
Objective: To insert dental implants with different insertion torque and analyze the influences of osseointegration between dental implants and surrounding bones. Methods: Select 4 healthy adult beagle dogs as experimental subjects, each dog will insert eight implants. In accordance with the Latin square experimental design methods, we can determine the insertion torque of each site: ≤15N·cm(group A), 25N·cm(group B), 35N·cm(group C)and≥45N·cm(group D). 32 implants were randomly divided into two groups:andⅡ. After 2 months, 4 dogs were sacrificed. The samples undergone HE staining as well as methylene blue acid fuchsin staining and then analyzed by optical microscope observation. Results: All implants were success. New bones growed more actively in groupⅠthan in group Ⅱ.A lot of osteoblast as well as bone matrix were found in group B and group C. Conclusions: For BLBⅢ, compared to insertion torque≤15N·cm(group A) and≥45N·cm(group D), implants with insertion torque of 25N·cm(group B)and 35N·cm(group C)have better osseointegration.
引文
1 Chopra A,Mhapuskar AA,Marathe S,et a1.Evaluation of Osseointegration in Implants using Digital Orthopantomogram and Cone Beam Computed Tomography[J].Contemp Dent Pract,2016,17(11):953-957
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2 Yoon WJ,Kim SG,Oh JS,et a1.Comparative study on the osseointegration of implants in dog mandibles according to the implant surface treatment[J].Korean Assoc Oral Maxillofac Surg.2016,42(6):345-351
3 Wada M,Suganami T,Sogo M,et a1.Can we predict the insertion torque using the bone density around the implant[J].Oral Maxillofac Surg.2016,45(2):221-225
4 Weng CC,Ou KL,Wu CY,et a1.Mechanism and Clinical Properties of Stem Bios Cell Therapy:Induction of Early Osseointegration in Novel Dental Implants[J].Oral Maxillofac Implants.2017,32(1):47-54
5 Poort LJ,Kiewiet CC,Cleutjens JP,et a1.Osseointegration and implant stability of extraoral Implants in Gottingen minipigs after irradiation[J].Craniomaxillofac Surg.2016,44(11):1842-1848
6 Rossi F,Botticelli D,Pantani F,et al.Bone healing pattern in surgically created circumferential defects around submerged implants:an experimental study in dog[J].Clin Oral Implants Res,2012,23(1):41-48
7 Ramos UD,Suaid FA,Wikesjo UM,et a1.Comparison between two antimicrobial protocols with or without guided bone regeneration in the treatment of peri-implantitis.A histomorphometric study in dogs[J].Clin Oral Implants Res.2017,43(1):1-8
8 De Santis E,Lang NP,Salata LA,et al.Healing of Bone CeramicTMat buccal dehiscence defects at implants installed immediately into extraction sockets.An experimental study in dogs[J].Clin Oral Implants Res.2016,27(11):1462-1468
9 Zheng X,Mo A,Wang Y,et al.Effect of FK-506(tacrolimus)therapy on bone healing of titanium implants:a histometric and biomechanical study in mice[J].2017,125(1):28-33
10 王东胜,刘洪臣,吕燕等.带种植体骨磨片的标准化制作[J].中华老年口腔医学杂志,2008,6(4):203-205
11 王东胜.三种特殊染色法显示不脱钙骨切片中新生骨的比较研究[J].中华老年口腔医学杂志,2006,4(3):139-141