不同植入扭矩对牙种植体-骨结合影响的动物实验研究
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摘要
目的:研究不同植入扭矩对牙种植体-骨结合的影响。方法:采用拉丁方实验设计及完全随机设计相结合的嵌套实验方法。4只beagle犬分别选取4个下颌前磨牙区植入位点,每个位点植入两枚BLBⅢ种植体,进行植入扭矩≤15 N·cm(A组)、25 N·cm(B组)、35 N·cm(C组)、≥45 N·cm(D组) 4组实验。32枚种植体根据植入时间点不同随机分为Ⅰ组(拔牙后即刻种植,8个位点)和Ⅱ组(Ⅰ组种植完成后1月种植,8个位点)。2个月时处死动物,处死前行四环素-茜素红双色荧光标记。标本进行X线检查、光学及荧光显微镜观察、定量组织学分析。结果:32枚种植体均无松动脱落,在Ⅰ、Ⅱ组中,B、C组的骨平均矿化率、种植体-骨结合率、骨结合指数均高于A、D组(P <0. 01)。结论:植入扭矩25 N·cm、35 N·cm较植入扭矩≤15 N·cm、≥45 N·cm更利于BLBⅢ牙种植体-骨结合。
Objective: To investigate the effects of different implant insertion torque on the osseointegration between dental implants and bone. Methods: 8 BLB Ⅲ implants were inserted into mandibular premolar area in each of 4 healthy adult beagle dogs. In accordance with the Latin square experimental design methods,insertion torque at each site( 2 implants were implanted at each site) was ≤15 N·cm( group A),25 N·cm( group B),35 N·cm( group C) and ≥45 N·cm( group D),respectively. 32 implants were randomly divided into groupⅠ( implantation immediately after tooth extraction) and Ⅱ( implantation 1 month after implantation of group Ⅰ). 2 months after implantation,4 dogs were sacrificed after tetracycline-alizarin red double color fluorescent labeling. The samples were analyzed by X-ray observation,optical microscope observation,fluorescence observation and quantitative histological analysis. Results: All implants were stably retained. The bone mineralization ration,implant-bone ingrowth fraction and osseointegration indexes of group B and C was higher than those of group A and D( P < 0. 01) in both group Ⅰ and Ⅱ. Conclusion: For BLB Ⅲ implants with insertion torque of 25 N·cm and 35 N·cm may induce better osseointegration than that of ≤15 N·cm and ≥45 N·cm.
Objective: To investigate the effects of different implant insertion torque on the osseointegration between dental implants and bone. Methods: 8 BLB Ⅲ implants were inserted into mandibular premolar area in each of 4 healthy adult beagle dogs. In accordance with the Latin square experimental design methods,insertion torque at each site( 2 implants were implanted at each site) was ≤15 N·cm( group A),25 N·cm( group B),35 N·cm( group C) and ≥45 N·cm( group D),respectively. 32 implants were randomly divided into groupⅠ( implantation immediately after tooth extraction) and Ⅱ( implantation 1 month after implantation of group Ⅰ). 2 months after implantation,4 dogs were sacrificed after tetracycline-alizarin red double color fluorescent labeling. The samples were analyzed by X-ray observation,optical microscope observation,fluorescence observation and quantitative histological analysis. Results: All implants were stably retained. The bone mineralization ration,implant-bone ingrowth fraction and osseointegration indexes of group B and C was higher than those of group A and D( P < 0. 01) in both group Ⅰ and Ⅱ. Conclusion: For BLB Ⅲ implants with insertion torque of 25 N·cm and 35 N·cm may induce better osseointegration than that of ≤15 N·cm and ≥45 N·cm.
引文
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[15] Chiang HJ,Hsu HJ,Peng PW,et al. Early bone response to machined,sandblasting acid etching(SLA)and novel surface-functionalization(SLAffinity)titanium implants:Characterization,biomechanical analysis and histological evaluation in pigs[J]. J Biomed Mater Res A,2016,104(2):397-405.
[2]赵刚,曾娟,李德超,等. h BMP2修饰的β-TCP/胶原支架对MC3T3-E1细胞成骨能力的影响[J].实用口腔医学杂志,2017,33(5):598-602.
[3] Yoon WJ,Kim SG,Oh JS,et al. Comparative study on the osseointegration of implants in dog mandibles according to the implant surface treatment[J]. J Korean Assoc Oral Maxillofac Surg,2016,42(6):345-351.
[4] Poort LJ,Kiewiet CC,Cleutjens JP,et al. Osseointegration and implant stability of extraoral implants in G9ttingen minipigs after irradiation[J]. J Craniomaxillofac Surg,2016,44(11):1842-1848.
[5] Marin C,Bonfante E,Granato R,et al. The effect of osteotomy dimension on implant insertion torque,healing mode,and osseointegration indicators:A study in dogs[J]. Implant Dent,2016,25(6):739-743.
[6]周炜,宋应亮,李德华,等.种植体骨结合稳定性测量及相关因素分析[J].实用口腔医学杂志,2006,22(6):803-806.
[7] Wada M,Suganami T,Sogo M,et al. Can we predict the insertion torque using the bone density around the implant?[J]. J Oral Maxillofac Surg,2016,45(2):221-225.
[8] Rossi F,Botticelli D,Pantani F,et al. Bone healing pattern in surgically created circumferential defects around submerged implants:An experimental study in dog[J]. Clin Oral Implants Res,2012,23(1):41-48.
[9] Ramos UD,Suaid FA,Wikesj9 UME,et al. Comparison between two antimicrobial protocols with or without guided bone regeneration in the treatment of peri-implantitis. A histomorphometric study in dogs[J]. Clin Oral Implants Res,2017,28(11):1388-1395.
[10] De Santis E,Lang NP,Salata LA,et al. Healing of BoneCeramicTMat buccal dehiscence defects at implants installed immediately into extraction sockets. An experimental study in dogs[J]. Clin Oral Implants Res,2016,27(11):1462-1468.
[11] Zheng X,Mo A,Wang Y,et al. Effect of FK-506(tacrolimus)therapy on bone healing of titanium implants:A histometric and biomechanical study in mice[J]. Eur J Oral Sci,2017,125(1):28-33.
[12] Cei S,Karapetsa D,Aleo E,et al. Protein adsorption on a laser-modified titanium implant surface[J]. Implant Dent,2015,24(2):134-141.
[13] Trisi P,Berardini M,Falco A,et al. Validation of value of actual micromotion as a direct measure of implant micromobility after healing(secondary implant stability). An in vivo histologic and biomechanical study[J]. Clin Oral Implant Res,2016,27(11):1423-1430.
[14] Sun SP,Lee DW,Yun JH,et al. Effects of thread depth in the neck area on peri-implant hard and soft tissues:An animal study[J]. J Periodontol,2016,87(11):1360-1368.
[15] Chiang HJ,Hsu HJ,Peng PW,et al. Early bone response to machined,sandblasting acid etching(SLA)and novel surface-functionalization(SLAffinity)titanium implants:Characterization,biomechanical analysis and histological evaluation in pigs[J]. J Biomed Mater Res A,2016,104(2):397-405.