影响农杆菌转化的植物因素研究进展
|
摘要
农杆菌作为一种天然植物转化体系,已经广泛应用于植物转基因研究中。在建立和优化转化体系研究中发现农杆菌转化法具有植物基因型依赖性,不同植物受体转化效率差异很大。在农杆菌转化的分子机制研究中发现有许多植物基因及蛋白质参与农杆菌的转化过程。在农杆菌转化过程中,植物的生理生化及基因表达等方面有很大变化,植物防御酶和内源激素变化对农杆菌转化效率有重要影响。本综述对近年来影响农杆菌转化的植物因素的研究进展进行评述,以期为提高农杆菌转化效率及探索农杆菌转化机制的研究提供理论依据和实践基础。
Agrobacterium tumefaciens as a natural tool for plant transformation, have been widely applied to plant transgenic researches. The research of constructing and optimizing transformation system suggested that the Agrobacterium-mediated transformation had high reliability on gene type, and the transformation efficiency was influenced by different receptors. The research of molecular mechanisms of Agrobacterium-mediated transformation indicated that many plant genes and proteins have involved in the process of transformation. In the process of Agrobacterium-mediated plant transformation, there were great changes in plant physiological and biochemical and genetic expression. Changing of plant defense enzymes and endogenous hormones had important effects on the transformation efficiency. The research progress of plant factors affecting Agrobacterium-mediated transformation in recent years was reviewed. And it would provide a theoretical and practical foundation for improving transformation efficiency and exploring transformation mechanism.
Agrobacterium tumefaciens as a natural tool for plant transformation, have been widely applied to plant transgenic researches. The research of constructing and optimizing transformation system suggested that the Agrobacterium-mediated transformation had high reliability on gene type, and the transformation efficiency was influenced by different receptors. The research of molecular mechanisms of Agrobacterium-mediated transformation indicated that many plant genes and proteins have involved in the process of transformation. In the process of Agrobacterium-mediated plant transformation, there were great changes in plant physiological and biochemical and genetic expression. Changing of plant defense enzymes and endogenous hormones had important effects on the transformation efficiency. The research progress of plant factors affecting Agrobacterium-mediated transformation in recent years was reviewed. And it would provide a theoretical and practical foundation for improving transformation efficiency and exploring transformation mechanism.
引文
Bourras S.,Rouxel T.,and Meyer M.,2015,Agrobacterium tumefaciens gene transfer:How a plant pathogen hacks the nuclei of plant and nonplant organisms,Phytopathology,105(10):1288-301
Chateau S.,Sangwan R.S.,and Sangwan-norreel B.S.,2000,Competence of Arabidopsis thaliana genotypes and mutants for Agrobacterium tumefaciens-mediated gene transfer:role of phytohormones,J.Exp.Bot.,51(353):1961-1968
Dan Y.H.,Armstrong C.L.,Dong J.,Feng X.R.,Fry J.E.,Keithly G.E.,Martinell B.J.,Roberts G.A.,Smith L.A.,Tan L.J.and Duncan D.R.,2009,Lipoic acid-an unique plant transformation enhancer,In Vitro Cell.Dev.Biol.Plant,45(6)630-638
Ditt R.F.,Nester E.W.,and Comai L.,2001,Plant gene expression response to Agrobacterium tumefaciens,Proc.Natl.A-cad.Sci.USA,98(19):10954-10959
Djamei A.,Pitzschke A.,Nakagami H.,Rajh I.,and Hirt H.2007,Trojan horse strategy in Agrobacterium transformation:abusing MAPK defense signaling,Science,318:453-456
García-cano E.,Hak H.,Magori S.,Lazarowitz S.,and Citovsky V.,2018,The Agrobacterium F-box protein effector Vir Fdestabilizes the Arabidopsis GLABROUS1 enhancer/binding protein-like transcription factor VFP4,a transcriptional activator of defense response genes,Mol.Plant Microbe Interact.,31(5):576-586
Gelvin S.B.,2010,Plant proteins involved in Agrobacterium-mediated genetic transformation,Annu.Rev.Phytopathol.,48(8):45-68
Gelvin S.B.,2017,Integration of Agrobacterium T-DNA into the plant genome,Annu.Rev.Genet.,51:195-217
Guo B.F.,Ma Y.S.,Zhang L.J.,Hong H.L.,Chang R.Z.,Guo Y.and Qiu L.J.,2015,Assessment of Agrobacterium sensitivity for different soybean genotypes and suitable germplasm screening,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),16(3):569-575(郭兵福,马岩松,张丽娟,洪慧龙,常汝真,郭勇,邱丽娟,2015,不同大豆基因型对农杆菌敏感性研究及优异种质筛选,植物遗传资源学报,16(3):569-575)
Gong Y.F.,and Gao F.,2008,Effects of Agrobacterium tumefaciens on the physiological and biochemical properties in sweetpotato explants,Zhongguo Nongye Kexue(Scientia A-gricultura Sinica),41(6):1649-1654(龚一富,高峰,2008根癌农杆菌感染对甘薯外植体生理生化特性的影响,中国农业科学,41(6):1649-1654)
Hansen G.,and Wright M.S.,1999,Recent advances in the transformation of plants,Trends Plant Sci.,4(6):226-231
Hou W.S.,Lin K.X.,Chen P.,Jia Z.W.,Zhou Y.,Yu Y.,and Liu Y.H.,2014,Establishment and application of large-scale transformation systems for soybean,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(21):4198-4210(侯文胜,林抗雪,陈普,贾志伟,周扬,于洋,刘雁华,2014,大豆规模化转基因技术体系的构建及其应用,中国农业科学,47(21):4198-4210)
Huang F.C.,Fu B.J.,Liu Y.T.,Chang Y.R.,Chi S.F.,Chien P.R.Huang S.C.,and Hwang H.H.,2018,Arabidopsis RETICU-LON-LIKE3(RTNLB3)and RTNLB8 participate in Agrobacterium-mediated plant transformation,Int.J.Mol.Sci.,19(3):638-659
Jia Y.Y.,2015,Effects of plant factors on Agrobacterium-mediated soybean cotyledonary node transformation,Dissertation for Ph.D.,Shenyang Agricultural University,Supervisors:Xie F.T.,and Yu C.M.,pp.38-96(贾钰莹,2015,植物因子对农杆菌介导大豆子叶节遗传转化的影响,博士学位论文,沈阳农业大学,导师:谢甫绨,于翠梅,pp.38-96)
Jia Y.Y.,Yao X.D.,Zhao M.Z.,Zhao Q.,Du Y.L.,Yu C.M.,and Xie F.T.,2015,Comparison of soybean transformation efficiency and plant factors affecting transformation during the Agrobacterium infection process,Int.J.Mol.Sci.,16(8):18522-18543
Jones H.D.,Doherty A.,and Wu H.X.,2005,Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat,Plant Methods,1(1):5
Lincoln C.,Turner J.,and Estelle M.,1992,Hormone-resistant mutants of Arabidopsis have an attenuated response to Agrobacterium strains,Plant Physiol.,98(3):979-983
Liu P.,and Nester E.W.,2006,Indoleacetic acid,a product of transferred DNA,inhibits vir gene expression and growth of Agrobacterium tumefaciens C58,Proc.Natl.Acad.Sci.US-A,103(12):4658-4662
Liu Y.J.,Jia Z.W.,Liu Y.,Zhang D.F.,and Li Y.,2014,Establishment and application of large-scale transformation systems for maize,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(21):4172-4182(刘允军,贾志伟,刘艳,张登峰,黎裕,2014,玉米规模化转基因技术体系构建及其应用,中国农业科学,47(21):4172-4182)
Magori S.,and Citovsky V.,2011a,Epigenetic control of A-grobacterium T-DNA integration,Biochim.Biophys.Acta,1809(8):388-394
Magori S.,and Citovsky V.,2011b,Agrobacterium counteracts host-induced degradation of its effector F-box protein,Sci.Signal.,4(195):ra69
Magori S.,and Citovsky V.,2012,The role of the ubiquitin-proteasome system in Agrobacterium tumefaciens-mediated genetic transformation of plants,Plant Physiol.,160(1):65-71
Morrell P.L.,Buckler E.S.,and Ross-Ibarra J.,2012,Crop genomics:advances and applications,Nat.Rev.Genet.,13(2):85-96
Olhoft P.M.,and Somers D.A.,2001,L-cysteine increases Agrobacterium-mediated TDNA delivery into soybean cotyledonary-node cells,Plant Cell Rep.,20(8):706-711
Olhoft P.M.,Flagel L.E.,Donovan C.M.,and Somers D.A.,2003,Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method,Planta,216(5):723-735
Song Z.Y.,Tian J.L.,Fu W.Z.,Li L.,Lu L.H.,Zhou L.,Shan Z.H.,Tang G.X.,and Shou H.X.,2013,Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability,Journal of Zhejiang University-Science B,14(4):289-198
Tie W.W.,Zhou F.,Wang L.,Xie W.B.,Chen H.,Li X.H.,and Lin Y.J.,2012,Reasons for lower transformation efficiency in indica rice using Agrobacterium tumefaciens-mediated transformation:lessons from transformation assays and genomewide expression profiling,Plant Mol.Biol.,78(1-2):1-18
van Kregten M.,de Pater S.,Romeijn R.,van Schendel R.,Hooykaas P.J.J.,and Tijsterman M.,2016,T-DNA integration in plants results from polymerase-θ-mediated DNA repair,Nature Plants,2(11):16164
Wang G.P.,Zhang T.,Shi Z.G.,and Cheng R.H.,2017,Research progress on T-DNA transfer related genes mediated of Agrobacterium,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding)15(5):1752-1761(王根平,张婷,师志刚,程汝宏,2017,农杆菌T-DNA传递相关基因研究进展,分子植物育种,15(5):1752-1761)
Wang Y.L.,Ye X.G.,Liu Y.P.,Du L.P.,and Xu H.J.,2005,Screening of wheat genotype sensitive to Agrobacterium tumefaciens infection,Mailei Zuowu Xuebao(Journal of Triticeae Crops),25(6):6-10(王艳丽,叶兴国,刘艳鹏,杜丽璞,徐惠君,2005,农杆菌敏感小麦基因型的筛选研究,麦类作物学报,25(6):6-10)
Wu S.,Xiao L.T.,Liu Q.,Shen G.Z.,and Wang R.Z.,2009,Relationship between endogenous hormones in rice calli and genetic transformation,Henongxue Bao(Journal of Nuclear Agricultural Sciences),23(2):257-261(吴顺,萧浪涛,刘清沈革志,王若仲,2009,水稻愈伤组织中内源植物激素水平与遗传转化的关系,核农学报,23(2):257-261)
Yamada T.,Watanabe S.,Arai M.,Harada K.,and Kitamura K.,2010,Cotyledonary node pre-wounding with a micro-brush increased frequency of Agrobacterium-mediated transformation in soybean,Plant Tiss.Cult.Lett.,27(2):217-220
Ye X.G.,Wang X.M.,Wang K.,Du L.P.,Lin Z.S.,and Xu H.J.,2012,A review of some assisted strategies for improving the efficiency of Agrobacterium-mediated plant transformation,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),45(15):3007-3019(叶兴国,王新敏,王轲,杜丽璞,林志珊,徐惠君,2012,提高植物农杆菌转化效率辅助策略研究进展,中国农业科学,45(15):3007-3019)
Ye X.G.,Xu H.J.,Du L.F.,He G.Y.,Wang K.,and Lin Z.P.,2014,Establishment and application of large-scale transformation systems in wheat,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(12):4155-4171(叶兴国,徐惠君,杜丽璞,何光源,王轲,林志珊,2014,小麦规模化转基因技术体系构建及其应用,中国农业科学,47(12):4155-4171)
Zeng L.H.,2001,Agrobacterium tumefaciens-mediated genetic transformation of litchi(Litchi chinensis Sonn.)with LEAFYgene,Dissertation for Ph.D.,Fujian Agriculture and Forestry University,Supervisor:Lv L.X.,pp.10-12(曾黎辉,2001,农杆菌介导荔枝(Litchi chinensis Sonn.)遗传转化体系的建立及导入LEAFY基因研究,博士学位论文,福建农林大学,导师:吕柳新,pp.10-12)
Zeng P.,Vadnais D.A.,Zhang Z.,and Polacco J.C.,2004,Refined glufosinate selection in,Agrobacterium-mediated transformation of soybean[Glycine max(L.)Merrill],Plant Cell Reports,22(7):478-482
Zhang D.X.,Zhang C.H.,and Li J.F.,2000,Embryogenic callus induction of maize leaves and related to endogenous IAAand ABA,Zuowu Xuebao(Acta Agronomica Sinica),26(2):195-199(张东向,张崇浩,李杰芬,2000,玉米叶片胚性愈伤组织诱导及其与内源IAA和ABA关系的初步研究,作物学报,26(2):195-199)
Zhang J.,Xu R.J.,Malcolm C.E.,and Chen D.F.,1997,Agrobacterium-mediated trans-formation of elite indica and jacanica rice cultivars,Mol.Biotechnol.,8(3):223-231
Zhang X.,Fu Y.P.,Zhou J.L.,Guo X.P.,Liu W.Z.,Wu J.F.,Wu C.Y.,and Wan J.M.,2014,Construction and application of rice large-scale transgenic technology system,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(2):4141-4154(张欣,付亚萍,周君莉,郭秀平,刘文真,吴洁芳,吴传银,万建民,2014,水稻规模化转基因技术体系构建与应用,中国农业科学,47(2):4141-4154)
Zhang Y.M.,Zhang H.M.,Liu Z.H.,Guo X.L.,Li H.C.,Li G.L.,Jiang C.Z.,and Zhang M.C.,2015,Inhibition of isoflavone biosynthesis enhanced T-DNA delivery in soybean by improving plant-Agrobacterium tumefaciens interaction,Plant Cell Tiss.Org.,121(1):183-193
Zhang Y.M.,Liu Z.H.,Yang R.J.,Li G.L.,Guo X.L.,Zhang H.N.,Zhang H.M.,Di R.,Zhao Q.S.,and Zhang M.C.,2016,Improvement of soybean transformation via Agrobacterium tumefaciens methods involvingα-aminooxyacetic acid and sonication treatments enlightened by gene expression profile analysis,Plant Cell Rep.,35(6):1259-1271
Zhao P.,Wang K.,Zhang W.,Du L.P.,and Ye X.G.,2014,Review and inspiration of plant proteins involved in the transformation processing of T-DNA initiated by Agrobacterium,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(13):2504-2518(赵佩,王轲,张伟,杜丽璞,叶兴国,2014,参与农杆菌侵染及T-DNA转运过程植物蛋白的研究进展和思考,中国农业科学,47(13):2504-2518)
Zhou X.H.,2014,Screening of Agrobacterium tumefaciens responsive gene in wheat and research of the function of TaUGT in the process of interaction between wheat and Agrobacterium,Dissertation for Ph.D.,Chinese Academy of Agricultural Sciences,Supervisor:Ye X.G.,pp.1-149(周晓鸿,2014,小麦应答农杆菌转化相关基因的筛选及TaUGT在小麦与农杆菌互作过程中的功能研究,博士学位论文,中国农业科学院,导师:叶兴国,pp.1-149)
Zhou X.H.,Wang K.,Du L.P.,Liu Y.W.,Lin Z.S.,and Ye X.G.,2017,Effects of the wheat UDP-glucosyltransferase gene TaUGT-B2 on Agrobacterium-mediated plant transformation,Acta Physiol.Plant.,39(1):15
Ziemienowicz A.,2014,Agrobacterium-mediated plant transformation:Factors,applications and recent advances,Biocatalysis and Agricultural Biotechnology,3(4):95-102
Chateau S.,Sangwan R.S.,and Sangwan-norreel B.S.,2000,Competence of Arabidopsis thaliana genotypes and mutants for Agrobacterium tumefaciens-mediated gene transfer:role of phytohormones,J.Exp.Bot.,51(353):1961-1968
Dan Y.H.,Armstrong C.L.,Dong J.,Feng X.R.,Fry J.E.,Keithly G.E.,Martinell B.J.,Roberts G.A.,Smith L.A.,Tan L.J.and Duncan D.R.,2009,Lipoic acid-an unique plant transformation enhancer,In Vitro Cell.Dev.Biol.Plant,45(6)630-638
Ditt R.F.,Nester E.W.,and Comai L.,2001,Plant gene expression response to Agrobacterium tumefaciens,Proc.Natl.A-cad.Sci.USA,98(19):10954-10959
Djamei A.,Pitzschke A.,Nakagami H.,Rajh I.,and Hirt H.2007,Trojan horse strategy in Agrobacterium transformation:abusing MAPK defense signaling,Science,318:453-456
García-cano E.,Hak H.,Magori S.,Lazarowitz S.,and Citovsky V.,2018,The Agrobacterium F-box protein effector Vir Fdestabilizes the Arabidopsis GLABROUS1 enhancer/binding protein-like transcription factor VFP4,a transcriptional activator of defense response genes,Mol.Plant Microbe Interact.,31(5):576-586
Gelvin S.B.,2010,Plant proteins involved in Agrobacterium-mediated genetic transformation,Annu.Rev.Phytopathol.,48(8):45-68
Gelvin S.B.,2017,Integration of Agrobacterium T-DNA into the plant genome,Annu.Rev.Genet.,51:195-217
Guo B.F.,Ma Y.S.,Zhang L.J.,Hong H.L.,Chang R.Z.,Guo Y.and Qiu L.J.,2015,Assessment of Agrobacterium sensitivity for different soybean genotypes and suitable germplasm screening,Zhiwu Yichuan Ziyuan Xuebao(Journal of Plant Genetic Resources),16(3):569-575(郭兵福,马岩松,张丽娟,洪慧龙,常汝真,郭勇,邱丽娟,2015,不同大豆基因型对农杆菌敏感性研究及优异种质筛选,植物遗传资源学报,16(3):569-575)
Gong Y.F.,and Gao F.,2008,Effects of Agrobacterium tumefaciens on the physiological and biochemical properties in sweetpotato explants,Zhongguo Nongye Kexue(Scientia A-gricultura Sinica),41(6):1649-1654(龚一富,高峰,2008根癌农杆菌感染对甘薯外植体生理生化特性的影响,中国农业科学,41(6):1649-1654)
Hansen G.,and Wright M.S.,1999,Recent advances in the transformation of plants,Trends Plant Sci.,4(6):226-231
Hou W.S.,Lin K.X.,Chen P.,Jia Z.W.,Zhou Y.,Yu Y.,and Liu Y.H.,2014,Establishment and application of large-scale transformation systems for soybean,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(21):4198-4210(侯文胜,林抗雪,陈普,贾志伟,周扬,于洋,刘雁华,2014,大豆规模化转基因技术体系的构建及其应用,中国农业科学,47(21):4198-4210)
Huang F.C.,Fu B.J.,Liu Y.T.,Chang Y.R.,Chi S.F.,Chien P.R.Huang S.C.,and Hwang H.H.,2018,Arabidopsis RETICU-LON-LIKE3(RTNLB3)and RTNLB8 participate in Agrobacterium-mediated plant transformation,Int.J.Mol.Sci.,19(3):638-659
Jia Y.Y.,2015,Effects of plant factors on Agrobacterium-mediated soybean cotyledonary node transformation,Dissertation for Ph.D.,Shenyang Agricultural University,Supervisors:Xie F.T.,and Yu C.M.,pp.38-96(贾钰莹,2015,植物因子对农杆菌介导大豆子叶节遗传转化的影响,博士学位论文,沈阳农业大学,导师:谢甫绨,于翠梅,pp.38-96)
Jia Y.Y.,Yao X.D.,Zhao M.Z.,Zhao Q.,Du Y.L.,Yu C.M.,and Xie F.T.,2015,Comparison of soybean transformation efficiency and plant factors affecting transformation during the Agrobacterium infection process,Int.J.Mol.Sci.,16(8):18522-18543
Jones H.D.,Doherty A.,and Wu H.X.,2005,Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat,Plant Methods,1(1):5
Lincoln C.,Turner J.,and Estelle M.,1992,Hormone-resistant mutants of Arabidopsis have an attenuated response to Agrobacterium strains,Plant Physiol.,98(3):979-983
Liu P.,and Nester E.W.,2006,Indoleacetic acid,a product of transferred DNA,inhibits vir gene expression and growth of Agrobacterium tumefaciens C58,Proc.Natl.Acad.Sci.US-A,103(12):4658-4662
Liu Y.J.,Jia Z.W.,Liu Y.,Zhang D.F.,and Li Y.,2014,Establishment and application of large-scale transformation systems for maize,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(21):4172-4182(刘允军,贾志伟,刘艳,张登峰,黎裕,2014,玉米规模化转基因技术体系构建及其应用,中国农业科学,47(21):4172-4182)
Magori S.,and Citovsky V.,2011a,Epigenetic control of A-grobacterium T-DNA integration,Biochim.Biophys.Acta,1809(8):388-394
Magori S.,and Citovsky V.,2011b,Agrobacterium counteracts host-induced degradation of its effector F-box protein,Sci.Signal.,4(195):ra69
Magori S.,and Citovsky V.,2012,The role of the ubiquitin-proteasome system in Agrobacterium tumefaciens-mediated genetic transformation of plants,Plant Physiol.,160(1):65-71
Morrell P.L.,Buckler E.S.,and Ross-Ibarra J.,2012,Crop genomics:advances and applications,Nat.Rev.Genet.,13(2):85-96
Olhoft P.M.,and Somers D.A.,2001,L-cysteine increases Agrobacterium-mediated TDNA delivery into soybean cotyledonary-node cells,Plant Cell Rep.,20(8):706-711
Olhoft P.M.,Flagel L.E.,Donovan C.M.,and Somers D.A.,2003,Efficient soybean transformation using hygromycin B selection in the cotyledonary-node method,Planta,216(5):723-735
Song Z.Y.,Tian J.L.,Fu W.Z.,Li L.,Lu L.H.,Zhou L.,Shan Z.H.,Tang G.X.,and Shou H.X.,2013,Screening Chinese soybean genotypes for Agrobacterium-mediated genetic transformation suitability,Journal of Zhejiang University-Science B,14(4):289-198
Tie W.W.,Zhou F.,Wang L.,Xie W.B.,Chen H.,Li X.H.,and Lin Y.J.,2012,Reasons for lower transformation efficiency in indica rice using Agrobacterium tumefaciens-mediated transformation:lessons from transformation assays and genomewide expression profiling,Plant Mol.Biol.,78(1-2):1-18
van Kregten M.,de Pater S.,Romeijn R.,van Schendel R.,Hooykaas P.J.J.,and Tijsterman M.,2016,T-DNA integration in plants results from polymerase-θ-mediated DNA repair,Nature Plants,2(11):16164
Wang G.P.,Zhang T.,Shi Z.G.,and Cheng R.H.,2017,Research progress on T-DNA transfer related genes mediated of Agrobacterium,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding)15(5):1752-1761(王根平,张婷,师志刚,程汝宏,2017,农杆菌T-DNA传递相关基因研究进展,分子植物育种,15(5):1752-1761)
Wang Y.L.,Ye X.G.,Liu Y.P.,Du L.P.,and Xu H.J.,2005,Screening of wheat genotype sensitive to Agrobacterium tumefaciens infection,Mailei Zuowu Xuebao(Journal of Triticeae Crops),25(6):6-10(王艳丽,叶兴国,刘艳鹏,杜丽璞,徐惠君,2005,农杆菌敏感小麦基因型的筛选研究,麦类作物学报,25(6):6-10)
Wu S.,Xiao L.T.,Liu Q.,Shen G.Z.,and Wang R.Z.,2009,Relationship between endogenous hormones in rice calli and genetic transformation,Henongxue Bao(Journal of Nuclear Agricultural Sciences),23(2):257-261(吴顺,萧浪涛,刘清沈革志,王若仲,2009,水稻愈伤组织中内源植物激素水平与遗传转化的关系,核农学报,23(2):257-261)
Yamada T.,Watanabe S.,Arai M.,Harada K.,and Kitamura K.,2010,Cotyledonary node pre-wounding with a micro-brush increased frequency of Agrobacterium-mediated transformation in soybean,Plant Tiss.Cult.Lett.,27(2):217-220
Ye X.G.,Wang X.M.,Wang K.,Du L.P.,Lin Z.S.,and Xu H.J.,2012,A review of some assisted strategies for improving the efficiency of Agrobacterium-mediated plant transformation,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),45(15):3007-3019(叶兴国,王新敏,王轲,杜丽璞,林志珊,徐惠君,2012,提高植物农杆菌转化效率辅助策略研究进展,中国农业科学,45(15):3007-3019)
Ye X.G.,Xu H.J.,Du L.F.,He G.Y.,Wang K.,and Lin Z.P.,2014,Establishment and application of large-scale transformation systems in wheat,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(12):4155-4171(叶兴国,徐惠君,杜丽璞,何光源,王轲,林志珊,2014,小麦规模化转基因技术体系构建及其应用,中国农业科学,47(12):4155-4171)
Zeng L.H.,2001,Agrobacterium tumefaciens-mediated genetic transformation of litchi(Litchi chinensis Sonn.)with LEAFYgene,Dissertation for Ph.D.,Fujian Agriculture and Forestry University,Supervisor:Lv L.X.,pp.10-12(曾黎辉,2001,农杆菌介导荔枝(Litchi chinensis Sonn.)遗传转化体系的建立及导入LEAFY基因研究,博士学位论文,福建农林大学,导师:吕柳新,pp.10-12)
Zeng P.,Vadnais D.A.,Zhang Z.,and Polacco J.C.,2004,Refined glufosinate selection in,Agrobacterium-mediated transformation of soybean[Glycine max(L.)Merrill],Plant Cell Reports,22(7):478-482
Zhang D.X.,Zhang C.H.,and Li J.F.,2000,Embryogenic callus induction of maize leaves and related to endogenous IAAand ABA,Zuowu Xuebao(Acta Agronomica Sinica),26(2):195-199(张东向,张崇浩,李杰芬,2000,玉米叶片胚性愈伤组织诱导及其与内源IAA和ABA关系的初步研究,作物学报,26(2):195-199)
Zhang J.,Xu R.J.,Malcolm C.E.,and Chen D.F.,1997,Agrobacterium-mediated trans-formation of elite indica and jacanica rice cultivars,Mol.Biotechnol.,8(3):223-231
Zhang X.,Fu Y.P.,Zhou J.L.,Guo X.P.,Liu W.Z.,Wu J.F.,Wu C.Y.,and Wan J.M.,2014,Construction and application of rice large-scale transgenic technology system,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(2):4141-4154(张欣,付亚萍,周君莉,郭秀平,刘文真,吴洁芳,吴传银,万建民,2014,水稻规模化转基因技术体系构建与应用,中国农业科学,47(2):4141-4154)
Zhang Y.M.,Zhang H.M.,Liu Z.H.,Guo X.L.,Li H.C.,Li G.L.,Jiang C.Z.,and Zhang M.C.,2015,Inhibition of isoflavone biosynthesis enhanced T-DNA delivery in soybean by improving plant-Agrobacterium tumefaciens interaction,Plant Cell Tiss.Org.,121(1):183-193
Zhang Y.M.,Liu Z.H.,Yang R.J.,Li G.L.,Guo X.L.,Zhang H.N.,Zhang H.M.,Di R.,Zhao Q.S.,and Zhang M.C.,2016,Improvement of soybean transformation via Agrobacterium tumefaciens methods involvingα-aminooxyacetic acid and sonication treatments enlightened by gene expression profile analysis,Plant Cell Rep.,35(6):1259-1271
Zhao P.,Wang K.,Zhang W.,Du L.P.,and Ye X.G.,2014,Review and inspiration of plant proteins involved in the transformation processing of T-DNA initiated by Agrobacterium,Zhongguo Nongye Kexue(Scientia Agricultura Sinica),47(13):2504-2518(赵佩,王轲,张伟,杜丽璞,叶兴国,2014,参与农杆菌侵染及T-DNA转运过程植物蛋白的研究进展和思考,中国农业科学,47(13):2504-2518)
Zhou X.H.,2014,Screening of Agrobacterium tumefaciens responsive gene in wheat and research of the function of TaUGT in the process of interaction between wheat and Agrobacterium,Dissertation for Ph.D.,Chinese Academy of Agricultural Sciences,Supervisor:Ye X.G.,pp.1-149(周晓鸿,2014,小麦应答农杆菌转化相关基因的筛选及TaUGT在小麦与农杆菌互作过程中的功能研究,博士学位论文,中国农业科学院,导师:叶兴国,pp.1-149)
Zhou X.H.,Wang K.,Du L.P.,Liu Y.W.,Lin Z.S.,and Ye X.G.,2017,Effects of the wheat UDP-glucosyltransferase gene TaUGT-B2 on Agrobacterium-mediated plant transformation,Acta Physiol.Plant.,39(1):15
Ziemienowicz A.,2014,Agrobacterium-mediated plant transformation:Factors,applications and recent advances,Biocatalysis and Agricultural Biotechnology,3(4):95-102