3种植物次生代谢物质对舞毒蛾生长发育的影响
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摘要
通过生物测定、拒食活性测定和酶活测定试验,分析了3种植物次生代谢物质对舞毒蛾3龄幼虫的毒杀、拒食效果及其保护酶CAT、SOD活性的影响。试验结果表明:苦参碱、氧化苦参碱和山奈酚对舞毒蛾幼虫均具有良好的毒杀和拒食活性。其中,苦参碱杀虫效果最好,质量浓度为2.000 mg/mL的苦参碱对幼虫72 h死亡率达到71.67%,而山奈酚拒食效果最强,1.000 mg/mL时对幼虫72 h拒食率达到70.53%;离体酶活试验发现,苦参碱和氧化苦参碱对离体CAT、SOD活性均有较强的抑制作用;而低浓度的山奈酚则可以激活2种保护酶的活性;体内酶活试验显示,3种植物次生代谢物质对幼虫体内CAT、SOD活性的抑制作用会随着处理后时间的延长而减弱,其中苦参碱和氧化苦参碱处理组的2种保护酶活性均呈现先减小后增大的趋势,在处理后48 h活性达到最低并显著低于同时期对照组(P<0.05),而山奈酚处理组CAT活性则呈现出先增大后减小的趋势。
In this study,the effects of three plant secondary metabolites on two protective enzymes( CAT and SOD) of Lymantria dispar were analyzed by bioassay,in vitro and in vivo enzyme activity assay and antifeedant activity assay. The results indicated that matrine,oxymatrine and kaempferol all possessed a highly insecticidal and antifeedant activity on 3 rd instar larvae of L. dispar,among which matrine had the strongest insecticidal activity,and the insecticidal rate reached 71. 67% at2. 000 mg/m L,while kaempferol had the strongest antifeedant activity,and the antifeedant rate reached 70. 53% at 1. 000 mg/m L. In vitro enzyme activity tests showed that activities of CAT and SOD were both inhibited by matrine and oxymatrine,while two protective enzymes were activated by kaempferol at a low concentration. In vivo enzyme activity assays indicated that with the increase of treatment time,the inhibition of three kinds of plant secondary metabolites to CAT and SOD activities of larvae was weakened,protective enzyme activities of matrine and oxymatrine treatment groups both showed a first decreasing and then increasing tendency,moreover,the activities reached the lowest at 48 h after treatment and was significantly lower than that of the control group( P<0. 05);The activities of kaempferol treatment groups showed a first increasing and then decreasing tendency.
In this study,the effects of three plant secondary metabolites on two protective enzymes( CAT and SOD) of Lymantria dispar were analyzed by bioassay,in vitro and in vivo enzyme activity assay and antifeedant activity assay. The results indicated that matrine,oxymatrine and kaempferol all possessed a highly insecticidal and antifeedant activity on 3 rd instar larvae of L. dispar,among which matrine had the strongest insecticidal activity,and the insecticidal rate reached 71. 67% at2. 000 mg/m L,while kaempferol had the strongest antifeedant activity,and the antifeedant rate reached 70. 53% at 1. 000 mg/m L. In vitro enzyme activity tests showed that activities of CAT and SOD were both inhibited by matrine and oxymatrine,while two protective enzymes were activated by kaempferol at a low concentration. In vivo enzyme activity assays indicated that with the increase of treatment time,the inhibition of three kinds of plant secondary metabolites to CAT and SOD activities of larvae was weakened,protective enzyme activities of matrine and oxymatrine treatment groups both showed a first decreasing and then increasing tendency,moreover,the activities reached the lowest at 48 h after treatment and was significantly lower than that of the control group( P<0. 05);The activities of kaempferol treatment groups showed a first increasing and then decreasing tendency.
引文
[1]陈澄宇,康志娇,高希武.昆虫对植物次生物质的代谢适应机制及其对昆虫抗药性的意义[J].昆虫学报,2015,58(10):1126-1139.
[2]李明,曾任森,骆世明.次生代谢产物在植物抵抗病虫为害中的作用[J].中国生物防治,2007,23(3):269-273.
[3]王景顺,吴秋芳,路志芳.植物次生代谢物与林木抗虫性研究进展[J].江苏农业科学,2015,43(8):4-7.
[4]孔垂华.21世纪植物化学生态学前沿领域[J].应用生态学报,2002,13(3):349-353.
[5]高微微,伶建明,郭顺星.植物次生代谢产物的生态学功能研究进展[J].中国药学杂志,2006,41(13):961-964.
[6]贾明慧,张辉,张爱华,等.植物次生代谢产物在国内农药开发方面的研究进展[J].中国植保导刊,2012,32(3):15-18.
[7]吴琼,谭娟娟,刘业明,等.植物生物碱杀虫剂研究进展[J].安徽农业科学,2011,39(15):9033-9034.
[8]胡会平,刘建慧,孙鑫,等.苦参碱及衍生物的生物活性和合成研究进展[J].鲁东大学学报(自然科学版),2015,31(4):339-345.
[9]张芳芳,郑一凡,祝慧娟,等.山奈酚和槲皮素对大鼠细胞色素P450酶活性的影响[J].浙江大学学报(医学版),2006(1):18-22.
[10]Zhang J F,Sun J Z,Wu Z B,et al.Identification of cotton varieties resistant to cannme spider mite and exploration of resistance mechanism[J].Acta Phytophylacica Sinica,1993,20(2):155-161.
[11]彭露,严盈,刘万学,等.植食性昆虫对植物的反防御机制[J].昆虫学报,2010,53(5):572-580.
[12]姜礅,孟昭军,严善春.用茉莉酸甲酯局部喷施长白落叶松苗对落叶松毛虫体内防御酶的影响[J].北京林业大学学报,2017,39(2):58-63.
[13]Felton G W,Tumlinson J H.Plant-insect dialogs:complex interactions at the plant-insect interface[J].Current Opinion in Plant Biology,2008,11(4):457-463.
[14]李周直,沈惠娟,蒋巧根,等.几种昆虫体内保护酶系统活力的研究[J].昆虫学报,1994,37(4):399-403.
[15]查黎春.3种农药对天幕毛虫、舞毒蛾超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的影响[D].哈尔滨:东北林业大学,2011.
[16]张凯,周艳涛,王皙玮,等.铜、镉胁迫对舞毒蛾排毒代谢酶的影响[J].北京林业大学学报,2016,38(2):61-67.
[17]问荣荣,马玲,董婉莹.鱼藤酮对舞毒蛾的毒杀作用[J].东北林业大学学报,2016,44(9):103-105,111.
[18]Robert L H,Melody A K,Daniel L R.Classification,genetic variation and pathogenicity of Lymantria dispar nucleopolyhedrovirus isolates from Asia,Europe,and North America[J].Journal of lnvertebrate Pathology,2014,116:27-35.
[19]Lazarevic J,Peric Mataruga V,Ivanovic J,et al.Host plant effects on the genetic variation and correlations in the individual performance of the Gypsy Moth[J].Functional Ecology,1998,12(1):141-148.
[20]丁吉同,唐桦,阿地力·沙塔尔,等.4种植物源杀虫剂对亚洲型舞毒蛾幼虫的毒性与拒食作用[J].南京林业大学学报(自然科学版),2013,37(4):80-84.
[21]王晓红,杨忠岐,张彦龙.灭幼脲对锈色粒肩天牛成虫生殖系统及取食产卵的影响[J].环境昆虫学报,2012,34(2):178-183.
[22]汪丽军,刘涛,董书军,等.磷化氢熏蒸对桔小实蝇氧化代谢体系的影响研究[J].中国农学通报,2013,29(33):351-357.
[23]张鑫乾,严俊鑫,杨杰莹,等.重瓣玫瑰诱导抗性对双斑萤叶甲成虫解毒酶的影响[J].东北林业大学学报,2014,42(5):125-128.
[24]Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Anal Biochem,1976,72(1/2):248-254.
[25]袁海滨,李玉双,赵欣阳,等.3种植物精油对绿豆象成虫的熏蒸及驱避活性[J].吉林农业大学学报,2017,39(1):28-31.
[26]袁海滨,王晶,赵欣阳,等.黄花蒿精油对绿豆象成虫体内酶活力的影响[J].吉林农业大学学报,2016,38(4):416-419.
[27]王晓丽,王予彤,段立清,等.四种植物酚类物质对舞毒蛾生长发育及繁殖的影响[J].昆虫学报,2014,57(7):831-836.
[28]胡晓,严善春,鲁艺芳,等.东北红豆杉次生代谢物对舞毒蛾拒食活性的影响[J].北京林业大学学报,2011,33(5):151-154.
[29]Hasspieler B M,Behar J V,Carlson D B,et al.Susceptibility of channel catfish(Ictalurus punctatus)and brown bullhead(Ameriurus nebulosus)to oxidative stress:a comparative study[J].Aquat Toxicol,1994,28(1/2):53-64.
[30]Stephensen E,Svavarsson J,Sturve J,et al.Biochemical indicators of pollution exposure in shorthorn sculpin(Myoxocephalus scorpius),caught in four harbours on the southwest coast of Iceland[J].Aquatic Toxicology,2000,48(4):431-422.
[31]赵妍.山奈酚对脂多糖诱导小鼠急性肺损伤的保护作用[D].哈尔滨:东北农业大学,2013.
[32]杨振德,朱麟,赵博光,等.苦豆草生物碱对分月扇舟蛾体内消化酶和保护酶活性的影响[J].北京林业大学学报,2006,28(1):80-83.
[2]李明,曾任森,骆世明.次生代谢产物在植物抵抗病虫为害中的作用[J].中国生物防治,2007,23(3):269-273.
[3]王景顺,吴秋芳,路志芳.植物次生代谢物与林木抗虫性研究进展[J].江苏农业科学,2015,43(8):4-7.
[4]孔垂华.21世纪植物化学生态学前沿领域[J].应用生态学报,2002,13(3):349-353.
[5]高微微,伶建明,郭顺星.植物次生代谢产物的生态学功能研究进展[J].中国药学杂志,2006,41(13):961-964.
[6]贾明慧,张辉,张爱华,等.植物次生代谢产物在国内农药开发方面的研究进展[J].中国植保导刊,2012,32(3):15-18.
[7]吴琼,谭娟娟,刘业明,等.植物生物碱杀虫剂研究进展[J].安徽农业科学,2011,39(15):9033-9034.
[8]胡会平,刘建慧,孙鑫,等.苦参碱及衍生物的生物活性和合成研究进展[J].鲁东大学学报(自然科学版),2015,31(4):339-345.
[9]张芳芳,郑一凡,祝慧娟,等.山奈酚和槲皮素对大鼠细胞色素P450酶活性的影响[J].浙江大学学报(医学版),2006(1):18-22.
[10]Zhang J F,Sun J Z,Wu Z B,et al.Identification of cotton varieties resistant to cannme spider mite and exploration of resistance mechanism[J].Acta Phytophylacica Sinica,1993,20(2):155-161.
[11]彭露,严盈,刘万学,等.植食性昆虫对植物的反防御机制[J].昆虫学报,2010,53(5):572-580.
[12]姜礅,孟昭军,严善春.用茉莉酸甲酯局部喷施长白落叶松苗对落叶松毛虫体内防御酶的影响[J].北京林业大学学报,2017,39(2):58-63.
[13]Felton G W,Tumlinson J H.Plant-insect dialogs:complex interactions at the plant-insect interface[J].Current Opinion in Plant Biology,2008,11(4):457-463.
[14]李周直,沈惠娟,蒋巧根,等.几种昆虫体内保护酶系统活力的研究[J].昆虫学报,1994,37(4):399-403.
[15]查黎春.3种农药对天幕毛虫、舞毒蛾超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性的影响[D].哈尔滨:东北林业大学,2011.
[16]张凯,周艳涛,王皙玮,等.铜、镉胁迫对舞毒蛾排毒代谢酶的影响[J].北京林业大学学报,2016,38(2):61-67.
[17]问荣荣,马玲,董婉莹.鱼藤酮对舞毒蛾的毒杀作用[J].东北林业大学学报,2016,44(9):103-105,111.
[18]Robert L H,Melody A K,Daniel L R.Classification,genetic variation and pathogenicity of Lymantria dispar nucleopolyhedrovirus isolates from Asia,Europe,and North America[J].Journal of lnvertebrate Pathology,2014,116:27-35.
[19]Lazarevic J,Peric Mataruga V,Ivanovic J,et al.Host plant effects on the genetic variation and correlations in the individual performance of the Gypsy Moth[J].Functional Ecology,1998,12(1):141-148.
[20]丁吉同,唐桦,阿地力·沙塔尔,等.4种植物源杀虫剂对亚洲型舞毒蛾幼虫的毒性与拒食作用[J].南京林业大学学报(自然科学版),2013,37(4):80-84.
[21]王晓红,杨忠岐,张彦龙.灭幼脲对锈色粒肩天牛成虫生殖系统及取食产卵的影响[J].环境昆虫学报,2012,34(2):178-183.
[22]汪丽军,刘涛,董书军,等.磷化氢熏蒸对桔小实蝇氧化代谢体系的影响研究[J].中国农学通报,2013,29(33):351-357.
[23]张鑫乾,严俊鑫,杨杰莹,等.重瓣玫瑰诱导抗性对双斑萤叶甲成虫解毒酶的影响[J].东北林业大学学报,2014,42(5):125-128.
[24]Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Anal Biochem,1976,72(1/2):248-254.
[25]袁海滨,李玉双,赵欣阳,等.3种植物精油对绿豆象成虫的熏蒸及驱避活性[J].吉林农业大学学报,2017,39(1):28-31.
[26]袁海滨,王晶,赵欣阳,等.黄花蒿精油对绿豆象成虫体内酶活力的影响[J].吉林农业大学学报,2016,38(4):416-419.
[27]王晓丽,王予彤,段立清,等.四种植物酚类物质对舞毒蛾生长发育及繁殖的影响[J].昆虫学报,2014,57(7):831-836.
[28]胡晓,严善春,鲁艺芳,等.东北红豆杉次生代谢物对舞毒蛾拒食活性的影响[J].北京林业大学学报,2011,33(5):151-154.
[29]Hasspieler B M,Behar J V,Carlson D B,et al.Susceptibility of channel catfish(Ictalurus punctatus)and brown bullhead(Ameriurus nebulosus)to oxidative stress:a comparative study[J].Aquat Toxicol,1994,28(1/2):53-64.
[30]Stephensen E,Svavarsson J,Sturve J,et al.Biochemical indicators of pollution exposure in shorthorn sculpin(Myoxocephalus scorpius),caught in four harbours on the southwest coast of Iceland[J].Aquatic Toxicology,2000,48(4):431-422.
[31]赵妍.山奈酚对脂多糖诱导小鼠急性肺损伤的保护作用[D].哈尔滨:东北农业大学,2013.
[32]杨振德,朱麟,赵博光,等.苦豆草生物碱对分月扇舟蛾体内消化酶和保护酶活性的影响[J].北京林业大学学报,2006,28(1):80-83.