针刺与穴位埋线对穴区胶原纤维的影响
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摘要
目的:对比针刺与穴位埋线在不同干预时间内对大鼠肝俞穴区胶原纤维变化的影响。方法:选取56只清洁级SD大鼠,随机分为空白组、针刺组和穴位埋线组,按照干预时间将针刺组和穴位埋线分为4天、7天和14天3个时间段亚组,每组8只。针刺组每日针刺右侧肝俞一次,深度至皮下4mm。穴位埋线组采用简易穴位埋线法将4mm长的可吸收缝合线埋入右侧肝俞穴。空白组予针刺组和穴位埋线组相同的麻醉、抓取和固定,不做其他处理。在干预4天、7天和14天后处死大鼠,取出右侧肝俞穴区肌肉组织进行HE染色、MASSON三色改良染色。镜下观察穴区深部肌纤维、胶原纤维形态与量的变化。结果:干预4天后,针刺组穴区胶原纤维较空白组与穴位埋线组均有一定程度的增加,有统计学意义(P<0.05)。干预7天、14天后,针刺组和穴位埋线组与空白组胶原纤维比较均具有显著性差异(P<0.01)。针刺4天、7天、14天后,针刺组穴区胶原纤维随着干预时间的延长而增多,其中4天组和14天组比较有统计学意义(P<0.05)。穴位埋线4天、7天、14天后,穴区胶原纤维也随着干预时间的延长而增多,7天、14天组与4天组比较具有显著性差异(P<0.01)。结论:针刺和穴位埋线在14天内随着干预时间的延长均可使穴区深部组织的胶原纤维增多。针刺组增生的胶原纤维主要在肌纤维变形、扭曲与断裂处,穴位埋线增生的胶原纤维主要沿着肌纤维长轴方向走行,分布较均匀。
Objective To compare the effects of acupuncture and catgut implantation on changes of the collagen fibers in Ganshu Points of rats at different intervention time. Method: 56 clean grade SD rats were randomly divided into a blank group,an acupuncture group and a catgut implantation group. The acupuncture group and the catgut implantation group were subdivided into 4-day,7-day and 14-day intervention subgroups respectively with8 rats in each group. Acupuncture was operated subcutaneously in the right Ganshu Points by the depth of 4 mm once a day in the acupuncture group. 4 mm long absorbable sutures were embedded in the same point in the catgut implantation group. The same anesthesia,grabbing and fixing treatments were performed to the blank group with no extra intervention. Rats were sacrificed on the 4 th,7 th and 17 th day of intervention and the muscle tissues of the right Ganshu Points were taken for HE staining and the improved MASSON staining. The changes of morphology and deep muscle and collagen fibers were observed under microscope. Results: A certain degree of increasing of connective tissue was observed in the acupuncture group after 4 day's intervention compared to the blank group and the catgut implantation group( P < 0.05). After 7 day's and 14 day's intervention,there were significant difference of Collagen fibers in the acupuncture group and the catgut implantation group compared with the blank group( P<0.01). After 4 day's,7 day's and 14 day's intervention,collagen fibers were increased in the acupuncture group with the prolongation of the intervention time. There were significant differences between 4-day and 14-day subgroups( P<0.05). After 4 day's,7 day's and 14 day's intervention,collagen fibers were also increased in the catgut implantation group with the prolongation of intervention time. A significant difference was observed in the 7-day and the 14-day subgroups compared with the 4-day subgroup( P<0.01). Conclusion: Collagen fibers were significantly increased along with the extension of the intervention time within 14 days in both the acupuncture and the catgut implantation group. The distribution of increased collagen fibers were mainly in the places of muscle fiber deformation,distortion and fracture in acupuncture group whereas the distribution in the catgut implantation group was even along the direction of the long axis of muscle fibers.
Objective To compare the effects of acupuncture and catgut implantation on changes of the collagen fibers in Ganshu Points of rats at different intervention time. Method: 56 clean grade SD rats were randomly divided into a blank group,an acupuncture group and a catgut implantation group. The acupuncture group and the catgut implantation group were subdivided into 4-day,7-day and 14-day intervention subgroups respectively with8 rats in each group. Acupuncture was operated subcutaneously in the right Ganshu Points by the depth of 4 mm once a day in the acupuncture group. 4 mm long absorbable sutures were embedded in the same point in the catgut implantation group. The same anesthesia,grabbing and fixing treatments were performed to the blank group with no extra intervention. Rats were sacrificed on the 4 th,7 th and 17 th day of intervention and the muscle tissues of the right Ganshu Points were taken for HE staining and the improved MASSON staining. The changes of morphology and deep muscle and collagen fibers were observed under microscope. Results: A certain degree of increasing of connective tissue was observed in the acupuncture group after 4 day's intervention compared to the blank group and the catgut implantation group( P < 0.05). After 7 day's and 14 day's intervention,there were significant difference of Collagen fibers in the acupuncture group and the catgut implantation group compared with the blank group( P<0.01). After 4 day's,7 day's and 14 day's intervention,collagen fibers were increased in the acupuncture group with the prolongation of the intervention time. There were significant differences between 4-day and 14-day subgroups( P<0.05). After 4 day's,7 day's and 14 day's intervention,collagen fibers were also increased in the catgut implantation group with the prolongation of intervention time. A significant difference was observed in the 7-day and the 14-day subgroups compared with the 4-day subgroup( P<0.01). Conclusion: Collagen fibers were significantly increased along with the extension of the intervention time within 14 days in both the acupuncture and the catgut implantation group. The distribution of increased collagen fibers were mainly in the places of muscle fiber deformation,distortion and fracture in acupuncture group whereas the distribution in the catgut implantation group was even along the direction of the long axis of muscle fibers.
引文
[1]赵丽云,张铭,赵福建,等.筋膜与经络研究进展[J].中华中医药杂志,2011,26(08):671-675.
[2]费伦,承焕生,蔡德亨,等.经络物质基础及其功能性特征的实验探索和研究展望[J].科学通报,1998,43(06):658-672.
[3]原林,焦培峰,唐雷,等.中医经络理论的物质基础——结缔组织、筋膜和自体监控系统[J].中国基础科学,2005,03(18):44-47,65.
[4]余晓佳,丁光宏,姚伟,等.穴位处胶原纤维在针刺大鼠“足三里”镇痛过程中的作用[J].中国针灸,2008,28(03):207-213.
[5]李程,陈波,胡铁汉,等.不同规格毫针针刺对大鼠穴区筋膜组织、细胞和胶原纤维的影响[J].中国针灸,2015,35(08):801-805.
[6]邓雪,任路,李静,等.电针“肝俞”穴对抑郁型胃溃疡大鼠胃窦黏膜、下丘脑组织P物质和海马5-羟色胺的影响[J].针刺研究,2014,39(02):124-129.
[7]石学敏.针灸学[M].北京:中国中医药出版社,2002:147-149.
[8]崔瑾.路绍祖教授应用简易穴位埋线法的经验[J].贵阳中医学院学报,1998,20(02):7-8.
[9]曾倩,陈志勇,李栋梁.Photoshop软件在瘢痕病理切片胶原纤维含量定量分析中的应用[J].中国美容医学,2017,26(01):93-95.
[10]陈波.毫针刺法基本微观力学作用的分析研究[J].贵阳中医学院学报,2011,33(02):32-35.
[11]孙文善,楚宁宁,封燕婷,等.超声引导下颈夹脊穴埋线层次与得气的初步研究[J].中国针灸,2015,35(09):931-934.
[12]梁欣,聂红昉,候珣瑞,等.运用磁共振T2图动态观察正常人体足三里穴微创埋线后线体对局部的刺激效应[J].针刺研究,2016,41(04):356-360.
[13]余晓璐,范刚启.针刺方向、角度、深度与偏头痛针刺疗效的关系[J].针灸临床杂志,2012,28(08):76-79.
[14]张传恩,杨丹,接瑞玲.毫针粗细对针灸阳经腧穴治疗脑梗死偏瘫疗效的影响[J].山东中医杂志,2015,34(06):441-442.
[15]吴松,梁凤霞,王华,等.试论针刺治疗量[J].中国针灸,2015,35(05):465-468.
[16]丛树园,毛晓雯,唐柱生,等.针刺肝俞深度角度的研究[J].中国民族民间医药,2014,23(16):51-52.
[17]翟中和,王喜忠,丁明孝,细胞生物学(4)[M].北京:高等教育出版社,2011:372-379.
[18]马忆南,杨华元,冯麟,等.针刺诱导胶原信号变化对骨骼肌损伤的修复作用[J].中国组织工程研究,2012,16(42):7888-7892.
[19]Ahn AC,Park M,Shaw JR,et al.Electrical impedance of acupuncture meridians:the relevance of subcutaneous collagenous bands[J].PLo S One.2010;5(7):e11907.
[20]Julias M,Edgar LT,Buettner HM,et al.An in vitro assay of collagen fiber alignment by acupuncture needle rotation[J].Biomed Eng Online.2008;7:19.
[21]王荣国,王云亭,肖诚,等.不同电流强度电针对兔骨骼肌钝器伤后改善微循环、抗纤维化和抗氧化能力的作用[J].中国康复医学杂志,2012,27(05):400-405.
[22]孔梅,张翔,叶梅聆,等.过度离心训练引起的骨骼肌肌束膜衔接盘结构域变化及针刺干预效应[J].生理学报,2017,69(01):17-32.
[23]刘文飞,谢光辉,刘宏伟.光生物调节作用对急性骨骼肌损伤修复不同阶段的影响[J].感染、炎症、修复,2017,18(01):60-64.
[2]费伦,承焕生,蔡德亨,等.经络物质基础及其功能性特征的实验探索和研究展望[J].科学通报,1998,43(06):658-672.
[3]原林,焦培峰,唐雷,等.中医经络理论的物质基础——结缔组织、筋膜和自体监控系统[J].中国基础科学,2005,03(18):44-47,65.
[4]余晓佳,丁光宏,姚伟,等.穴位处胶原纤维在针刺大鼠“足三里”镇痛过程中的作用[J].中国针灸,2008,28(03):207-213.
[5]李程,陈波,胡铁汉,等.不同规格毫针针刺对大鼠穴区筋膜组织、细胞和胶原纤维的影响[J].中国针灸,2015,35(08):801-805.
[6]邓雪,任路,李静,等.电针“肝俞”穴对抑郁型胃溃疡大鼠胃窦黏膜、下丘脑组织P物质和海马5-羟色胺的影响[J].针刺研究,2014,39(02):124-129.
[7]石学敏.针灸学[M].北京:中国中医药出版社,2002:147-149.
[8]崔瑾.路绍祖教授应用简易穴位埋线法的经验[J].贵阳中医学院学报,1998,20(02):7-8.
[9]曾倩,陈志勇,李栋梁.Photoshop软件在瘢痕病理切片胶原纤维含量定量分析中的应用[J].中国美容医学,2017,26(01):93-95.
[10]陈波.毫针刺法基本微观力学作用的分析研究[J].贵阳中医学院学报,2011,33(02):32-35.
[11]孙文善,楚宁宁,封燕婷,等.超声引导下颈夹脊穴埋线层次与得气的初步研究[J].中国针灸,2015,35(09):931-934.
[12]梁欣,聂红昉,候珣瑞,等.运用磁共振T2图动态观察正常人体足三里穴微创埋线后线体对局部的刺激效应[J].针刺研究,2016,41(04):356-360.
[13]余晓璐,范刚启.针刺方向、角度、深度与偏头痛针刺疗效的关系[J].针灸临床杂志,2012,28(08):76-79.
[14]张传恩,杨丹,接瑞玲.毫针粗细对针灸阳经腧穴治疗脑梗死偏瘫疗效的影响[J].山东中医杂志,2015,34(06):441-442.
[15]吴松,梁凤霞,王华,等.试论针刺治疗量[J].中国针灸,2015,35(05):465-468.
[16]丛树园,毛晓雯,唐柱生,等.针刺肝俞深度角度的研究[J].中国民族民间医药,2014,23(16):51-52.
[17]翟中和,王喜忠,丁明孝,细胞生物学(4)[M].北京:高等教育出版社,2011:372-379.
[18]马忆南,杨华元,冯麟,等.针刺诱导胶原信号变化对骨骼肌损伤的修复作用[J].中国组织工程研究,2012,16(42):7888-7892.
[19]Ahn AC,Park M,Shaw JR,et al.Electrical impedance of acupuncture meridians:the relevance of subcutaneous collagenous bands[J].PLo S One.2010;5(7):e11907.
[20]Julias M,Edgar LT,Buettner HM,et al.An in vitro assay of collagen fiber alignment by acupuncture needle rotation[J].Biomed Eng Online.2008;7:19.
[21]王荣国,王云亭,肖诚,等.不同电流强度电针对兔骨骼肌钝器伤后改善微循环、抗纤维化和抗氧化能力的作用[J].中国康复医学杂志,2012,27(05):400-405.
[22]孔梅,张翔,叶梅聆,等.过度离心训练引起的骨骼肌肌束膜衔接盘结构域变化及针刺干预效应[J].生理学报,2017,69(01):17-32.
[23]刘文飞,谢光辉,刘宏伟.光生物调节作用对急性骨骼肌损伤修复不同阶段的影响[J].感染、炎症、修复,2017,18(01):60-64.