HS-SPME/GC-MS法测定榛蘑中挥发性成分
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摘要
为分析干品榛磨的挥发性成分,本文选用PDMS/DVB萃取头,应用顶空固相微萃取技术(HS-SPME),结合气相色谱-质谱检测方法(GC-MS),鉴定榛蘑中的挥发性成分,并以化合物匹配度≥90%为定性标准,采用峰面积归一化法计算各化学成分的相对质量分数。结果表明,SPME-GC-MS法可以较好的分离鉴定榛蘑中的挥发性成分。从榛蘑中鉴定出39种香气成分,占其总香气成分的88.74%,其中含酯类物质15种,含量为54.9%,酸类物质8种,含量为12.97%,芳香类物质4种,含量为7.25%。含量最高的三种化合物为顺-9-十八碳烯酸甲酯,9,12-十八碳二烯酸甲酯和油酸乙酯,含量分别为13.93%、12.7%和8.74%。研究证明弱极性的大分子酯类成分是干燥榛蘑的主要挥发性成分,为榛蘑干品的品质评价提供依据。
To analysis the volatile components in Armillaria mellea.PDMS/DVB fiber was used as extraction fiber.HP-SPMEGC-MS was used to identified the components of volatile in Armillaria mellea. Matching degree( ≥ 90%) was chosen as qualitative standard. Peak area normalization method was chosen as calculation method of the relative content of chemical compositions.The results showed that HS-SPME-GC-MS had a good performance of isolation and identification of volatile components in Armillaria mellea.39 kinds of aroma components,which account for 88.74% of total aroma components,were identified.Among all the aroma components,15 kinds of ester substances were included,occupying 54.9% of the total.8 acids of aromatic substances were included,occupying 12.97% of the total.4 aromatic components were included,occupying 7.25% of the total.The three compounds with the highest proportion are respectively Cis-9-eighteen octadecadenoate,9,12-eighteen octadecadienoic,and ethyl whose contents were 13.93%,12.7% and 8.74% of the total respectively. The experiment showed that the main components in the volatile components of dried Armillaria mellea were the large molecular ester compounds.These results could provide the base for quality evaluation of dried Armillaria mellea.
To analysis the volatile components in Armillaria mellea.PDMS/DVB fiber was used as extraction fiber.HP-SPMEGC-MS was used to identified the components of volatile in Armillaria mellea. Matching degree( ≥ 90%) was chosen as qualitative standard. Peak area normalization method was chosen as calculation method of the relative content of chemical compositions.The results showed that HS-SPME-GC-MS had a good performance of isolation and identification of volatile components in Armillaria mellea.39 kinds of aroma components,which account for 88.74% of total aroma components,were identified.Among all the aroma components,15 kinds of ester substances were included,occupying 54.9% of the total.8 acids of aromatic substances were included,occupying 12.97% of the total.4 aromatic components were included,occupying 7.25% of the total.The three compounds with the highest proportion are respectively Cis-9-eighteen octadecadenoate,9,12-eighteen octadecadienoic,and ethyl whose contents were 13.93%,12.7% and 8.74% of the total respectively. The experiment showed that the main components in the volatile components of dried Armillaria mellea were the large molecular ester compounds.These results could provide the base for quality evaluation of dried Armillaria mellea.
引文
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[9]陈智毅,刘学铭,施英,等.顶空固相微萃取气质联用分析白金针菇中的挥发性成分[J].食用菌学报,2009,16(1):73-75.
[2]JS Yang,YW Chen,XZ Feng,et al.Chemical constituents of Armillaria mellea mycelium I.Isolation and characterization of armillarin and armillaridin[J].Planta Medica,1984,50:288.
[3]JS Yang,YW Chen,XZ Feng,et al.Chemical constituents of Armillaria mellea myceliumⅡ.Isolation and structural elucidation of armillaricin[J].Ibis,1989,55:564.
[4]JS Yang,YW Chen,XZ Feng,et al.Chemical constituents of Armillaria mellea myceliumⅣ.Isolation and structures armillarigin and armillarikin[J].Ibis,1989,55:479.
[5]Yang JS,Su YL,Wang YL,et al Chemical constituents of Armillaria mellea mycelium.VI.Isolation and structure of armillaripin[J].Acta Pharmaceutica Sinica,1990,05:353-356.
[6]Sides S,Robards K,Helliwell S.Developments in extraction techniques and their application to analysis of volatiles in foods[J].Trends in Analytical Chemistry,2000,19:322-329.
[7]Mauriello G,Marino R,D’Auria M,et al.Determination of volatile organic compounds from truffles via SPME-GC-MS[J].Journal of Chromatographic Science,2004,42:299-305.
[8]谷镇,杨焱,冯涛,等.固相微萃取结合GC/MS联用技术研究野松茸干品的挥发性成分[J].天然产物研究与开发,2012,24(8):1063-1066.
[9]陈智毅,刘学铭,施英,等.顶空固相微萃取气质联用分析白金针菇中的挥发性成分[J].食用菌学报,2009,16(1):73-75.