反硝化菌对烟气汞吸附特性研究
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摘要
采用反硝化菌对烟气中Hg~0、Hg~(2+)的吸附特性进行研究,反硝化菌对Hg~0和Hg~(2+)吸附性能良好,在pH为8、初始Hg~0(Hg~(2+))浓度为95.7μg·m-3(0.4μg·L-1)和吸附剂用量为0.35 g·L-1时,Hg~0和Hg~(2+)的吸附效率分别达到43.01%和98.12%.反硝化菌吸附Hg~0的过程遵循拟一级动力学,符合Langmuir等温吸附模型,最大吸附容量为126.1μg·g-1.反硝化菌吸附Hg~(2+)遵循拟二级动力学模型,符合Langmuir等温吸附模型,最大吸附容量为36.23μg·g-1.采用傅里叶红外光谱(FTIR)和热场发射环境扫描电镜-能谱电子背散射衍射系统(EDS)表征吸附前后的反硝化菌,结果表明吸附过程中细菌表面的糖环、磷酸及脂肪化合物基团发挥了吸附汞作用.
The biosorption of Hg~0 and Hg~(2+)from flue gas by denitrifying bacteria was investigated in this study. The results show that the denitrifying bacteria had good adsorption performance for Hg~0 and Hg~(2+). The biosorption efficiencies of Hg~0 and Hg~(2+)reached 43. 01% and 98. 12%,respectively,under pH 8,initial Hg~0( Hg~(2+)) concentration of 95.7 μg·m-3( 0.4 μg·L-1) and the adsorbent dosage of 0.35 g·L-1. The adsorption process of Hg~0 and Hg~(2+)by denitrifying bacteria follows the Pseudo-first-order and pseudo-second-order kinetics model,respectively,and also Langmuir isotherm adsorption model. The maximal adsorption capacity was 126.1 and 36. 23 μg·g-1 for Hg~0 and Hg~(2+),respectively. The denitrifying bacteria was characterized with infrared spectrum( IR) and energy dispersive spectrometer( EDS) before and after adsorption. The results show that the sugar ring,phosphoric acid and fatty compound groups on the cell surface of denitrifying bacteria may have great effect on the adsorption of mercury.
The biosorption of Hg~0 and Hg~(2+)from flue gas by denitrifying bacteria was investigated in this study. The results show that the denitrifying bacteria had good adsorption performance for Hg~0 and Hg~(2+). The biosorption efficiencies of Hg~0 and Hg~(2+)reached 43. 01% and 98. 12%,respectively,under pH 8,initial Hg~0( Hg~(2+)) concentration of 95.7 μg·m-3( 0.4 μg·L-1) and the adsorbent dosage of 0.35 g·L-1. The adsorption process of Hg~0 and Hg~(2+)by denitrifying bacteria follows the Pseudo-first-order and pseudo-second-order kinetics model,respectively,and also Langmuir isotherm adsorption model. The maximal adsorption capacity was 126.1 and 36. 23 μg·g-1 for Hg~0 and Hg~(2+),respectively. The denitrifying bacteria was characterized with infrared spectrum( IR) and energy dispersive spectrometer( EDS) before and after adsorption. The results show that the sugar ring,phosphoric acid and fatty compound groups on the cell surface of denitrifying bacteria may have great effect on the adsorption of mercury.
引文
Al-Ghouti M A,Abuqaoud R H,Abu-Dieyeh M H.2016.Detoxification of mercury pollutant leached from spent fluorescent lamps using bacterial strains[J].Waste Management,49:238-244
Beckers F,Rinklebe J.2017.Cycling of mercury in the environment:source,fate,and human health implications:a review[J].Crit Rev Environ Sci Technol,47(10):00-00
Chojnacka K.2010.Biosorption and bioaccumulation--the prospects for practical applications[J].Environment International,36(3):299-307
Colombo M J,Ha J,Reinfelder J R,et al.2013.Anaerobic oxidation of Hg(0)and methylmercury formation by Desulfovibrio desulfuricans,ND132[J].Geochimica Et Cosmochimica Acta,112(7):166-177
Compeau G C,Bartha R.1985.Sulfate-Reducing Bacteria:Principal Methylators of Mercury in Anoxic Estuarine Sediment[J].Applied&Environmental Microbiology,50(2):498-502
Cui H,Li F,Ren B,et al.2018.Biosorption of aquatic Pb2+,Hg2+,and Cd2+using a combined biosorbent-Aspergillus niger-Treated Rice Straw[J].Separation Science&Technology,53(4):626-635
曹文超,郭景恒,宋贺,等.2017.设施菜田土壤p H和初始C/NO3对反硝化产物比的影响[J].植物营养与肥料学报,23(5):1249-1257
丁文,吴胜春,朱成,等.2018.水稻响应重金属胁迫的蛋白质组学研究进展[J].环境污染与防治,40(1):95-99
杜辉辉.2017.Cd(Ⅱ)、Pb(Ⅱ)在土壤矿物-有机互作界面的分子结合机制[D].武汉:华中农业大学
Dash H R,Das S.2012.Bioremediation of mercury and the importance of bacterial mer,genes[J].International Biodeterioration&Biodegradation,75(6):207-213
Delgado A,Anselmo A M,Novais J M.1998.Heavy metal biosorption by dried powdered mycelium of fusarium flocciferum[J].Water Environment Research,70(3):370-375
Esposito A,Pagnanelli F,Lodi A,et al.2001.Biosorption of heavy metals by Sphaerotilus natans:an equilibrium study at different p Hand biomass concentrations[J].Hydrometallurgy,60(2):129-141
The European Parliament and the Council.2013.Directive 2013/38/EUof the European Parliament and of the Council of 12 August 2013[J].Official Journal of the European Communities,L226
Filali B K,Taoufik J,Zeroual Y,et al.2000.Waste water bacterial isolates resistant to heavy metals and antibiotics[J].Current Microbiology,41(3):151-156
Gavrilescu M.2004.Removal of heavy metals from the environment by biosorption[J].Engineering in Life Sciences,4(3):219-232
GY Kholodii,et al.2000.Pseudomonas putida partial transposon.[J].Russian Journal of Genetics,36(4):459-469
高圣博,辛嘉英,窦博鑫,等.2017.甲烷氧化菌素催化二价汞还原的研究[J].化学工程师,31(11):1-6
He Z,Gao F,Sha T,et al.2009.Isolation and characterization of a Cr(VI)-reduction Ochrobactrum sp.strain CSCr-3 from chromium landfill[J].Journal of Hazardous Materials,163(2/3):869-873
Jia L,Li B,Xu L,et al.2018.Effects of different preparation conditions on kinetics and adsorption of mercury by biomass char[J].Chinese Journal of Environmental Engineering,12(1):134-144
KadukováJ,Vir c'íkováE.2005.Comparison of differences between copper bioaccumulation and biosorption[J].Environment International,31(2):227-232
Kamnev A A,Ristic'M,Antonyuk L P,et al.1997.Fourier transform infrared spectroscopic study of intact cells of the nitrogen-fixing bacterium Azospirillum brasilense[J].Journal of Molecular Structure,408(408):201-205
Loukidou M X,2005.Zouboulis A I.Biosorption of Toxic Metals[M].Water Encyclopedia.John Wiley&Sons,Inc
梁彩柳.2016.一株海洋类产碱假单胞菌的汞抗性及富集行为机制研究[D].深圳:深圳大学
梁龄予,王耀晶,闫颖,等.2015.玉米芯吸附水中Cr(Ⅵ)的特性及SEM-EDS表征分析[J].生态环境学报,24(2):305-309
刘文静.2017.抗汞菌株的筛选检测以及抗汞蛋白结构与功能的研究[D].合肥:安徽大学
吴鹏宇,夏令.2017.活性污泥胞外聚合物吸附重金属离子的研究进展[J].绿色科技,(16):75-80
Mahbub K R,Krishnan K,Naidu R,et al.2016.Mercury resistance and volatilization by Pseudoxanthomonas,sp.SE1 isolated from soil[J]Environmental Technology&Innovation,6:94-104
Mahbub K R,Krishnan K,Naidu R,et al.2017.Mercury remediation potential of a mercury resistant strain Sphingopyxis sp.SE2 isolated from contaminated soil[J].Journal of Environmental Sciences,51(1):128-137
Majumdar S S,Das S K,Chakravarty R,et al.2010.A study on lead adsorption by Mucor rouxii,biomass[J].Desalination,251(1/3):96-102
Mukhopadhyay M,Noronha S B,Suraishkumar G K.2007.Kinetic modeling for the biosorption of copper by pretreated Aspergillus niger,biomass[J].Bioresource Technology,98(9):1781-1787
Kumar R,Bishnoi N R,Garima,et al.Biosorption of chromium(VI)from aqueous solution and electroplating wastewater using funga biomass[J].Chemical Engineering Journal,135(3):202-208
Osborn A M,Bruce K D,Strike P,et al.1997.Distribution,diversity and evolution of the bacterial mercury resistance(mer)operon.[J]Fems Microbiology Reviews,19(4):239-262
Palinka L A,Sholupov S E,Mashyanov N R,et al.2010.Globa mercury emissions to the atmosphere from anthropogenic and natura sources[J].Atmospheric Chemistry&Physics Discussions,10(13):5951-5964
Qin H,Xiao R,Guo L,et al.2018.Mercury(II)adsorption from aqueous solution using nitrogen and sulfur co-doped activated carbon[J].Water Science and Technology,:wst2018117
Sruthy S,Mohan M,Binish M B.2018.Biosorption of mercury by meta tolerant bacteria from arctic using FT-IR spectroscopy[C].International Conference on Photo-Chemistry and ITS Applications
孙创,宁平,冯权莉,等.2014.含汞排放物处理技术的研究进展[J].现代化工,34(10):49-52
孙阳昭,陈扬,蓝虹,等.2016.中国汞污染的来源、成因及控制技术路径分析[J].环境化学,32(6):937-942
Veglio F,Beolchini F.1997.Removal of metals by biosorption:a review[J].Hydrometallurgy,44(3):301-316
Wei Z S,Wang J B,Huang Z S,et al.2017.Effect of gaseous mercury on nitric oxide removal performance and microbial community of a hybrid catalytic membrane biofilm reactor[J].Chemical Engineering Journal,316:584-591
王建龙,陈灿.2015.重金属生物吸附[M].北京:科学出版社
王佩琦,周伟丽,何圣兵,等.2018.磷对混养反硝化污泥活性和微生物群落结构的影响[J].环境科学,39(3):1350-1356
文晓凤,杜春艳,袁瀚宇,等.2016.改性磁性纳米颗粒固定内生菌Bacillus nealsonii吸附废水中Cd2+的特性研究[J].环境科学学报,36(12):4376-4383
文晓凤,杜春艳,袁瀚宇,等.2016.改性磁性纳米颗粒固定内生菌Bacillus nealsonii吸附废水中Cd2+的特性研究[J].环境科学学报,36(12):4376-4383
吴玉海.2018.工业废水废气治理方法研究[J].信息记录材料,(1):22-23
武振华,孙津生,宋慧平,等.2006.趋磁细菌(MTB)吸附Pd2+的实验研究[J].离子交换与吸附,22(5):385-391
奚涛.2015.重金属汞、铜、铅、镉的酶联免疫吸附快速检测方法研究[D].上海:上海交通大学
熊建林.2016.(NH4)2S法同时脱除冶炼烟气中Hg和SO2[D].昆明:昆明理工大学
Xia L I,Dan Z,Fei S,et al.2017.Biosorption of mercury(Hg2+)from water by immobilized residues from four types of edible mushroom[J].Chinese Journal of Applied&Environmental Biology,23(5):879-885
Yu Z,Li J,Li Y,et al.2014.A mer,operon confers mercury reduction in a Staphylococcus epidermidis,strain isolated from Lanzhou reach of the Yellow River[J].International Biodeterioration&Biodegradation,90(1):57-63
杨天伟,张霁,李涛,等.2016.红外光谱结合化学计量学方法快速鉴别牛肝菌种类及总汞含量分析[J].光谱学与光谱分析,36(11):3510-3516
杨文,陈小敏,朱保虎,等.2017.一株耐汞菌的分离鉴定及其去汞特性[J].环境工程学报,11(1):602-607
杨昕.2016.生物质与煤混合燃烧过程中汞的释放特性研究[D].北京:华北电力大学
姚海燕,李洋,龙飞,等.2016.电子废弃物处理场地重金属污染土壤的钝化修复及其机理初析[J].上海第二工业大学学报,33(2):127-133
Zhang Y,Cui Y,Chen L Y.2012.Mercury inhibits the L170C mutant of aquaporin Z by making waters clog the water channel[J].Biophysical Chemistry,160(1):69-74
Zheng W,Liang L,Gu B.2012.Mercury reduction and oxidation by reduced natural organic matter in anoxic environments[J].Environmental Science&Technology,46(1):292-299
张淑楠,王心义,夏大平.2015.初始p H值对微生物反硝化的影响[J].环境工程,(s1):67-71
张霞忠,袁烈梅.2017.汞吸附研究进展[J].环境污染与防治,39(5):563-568
张志,任洪强,张蓉蓉,等.2005.p H值对好氧颗粒污泥同步硝化反硝化过程的影响[J].中国环境科学,25(6):650-654
赵莉,刘宇,吴洋文,等.2018.燃煤烟气中零价汞的催化氧化理论研究进展[J].中国电力,51(3):170-176
Beckers F,Rinklebe J.2017.Cycling of mercury in the environment:source,fate,and human health implications:a review[J].Crit Rev Environ Sci Technol,47(10):00-00
Chojnacka K.2010.Biosorption and bioaccumulation--the prospects for practical applications[J].Environment International,36(3):299-307
Colombo M J,Ha J,Reinfelder J R,et al.2013.Anaerobic oxidation of Hg(0)and methylmercury formation by Desulfovibrio desulfuricans,ND132[J].Geochimica Et Cosmochimica Acta,112(7):166-177
Compeau G C,Bartha R.1985.Sulfate-Reducing Bacteria:Principal Methylators of Mercury in Anoxic Estuarine Sediment[J].Applied&Environmental Microbiology,50(2):498-502
Cui H,Li F,Ren B,et al.2018.Biosorption of aquatic Pb2+,Hg2+,and Cd2+using a combined biosorbent-Aspergillus niger-Treated Rice Straw[J].Separation Science&Technology,53(4):626-635
曹文超,郭景恒,宋贺,等.2017.设施菜田土壤p H和初始C/NO3对反硝化产物比的影响[J].植物营养与肥料学报,23(5):1249-1257
丁文,吴胜春,朱成,等.2018.水稻响应重金属胁迫的蛋白质组学研究进展[J].环境污染与防治,40(1):95-99
杜辉辉.2017.Cd(Ⅱ)、Pb(Ⅱ)在土壤矿物-有机互作界面的分子结合机制[D].武汉:华中农业大学
Dash H R,Das S.2012.Bioremediation of mercury and the importance of bacterial mer,genes[J].International Biodeterioration&Biodegradation,75(6):207-213
Delgado A,Anselmo A M,Novais J M.1998.Heavy metal biosorption by dried powdered mycelium of fusarium flocciferum[J].Water Environment Research,70(3):370-375
Esposito A,Pagnanelli F,Lodi A,et al.2001.Biosorption of heavy metals by Sphaerotilus natans:an equilibrium study at different p Hand biomass concentrations[J].Hydrometallurgy,60(2):129-141
The European Parliament and the Council.2013.Directive 2013/38/EUof the European Parliament and of the Council of 12 August 2013[J].Official Journal of the European Communities,L226
Filali B K,Taoufik J,Zeroual Y,et al.2000.Waste water bacterial isolates resistant to heavy metals and antibiotics[J].Current Microbiology,41(3):151-156
Gavrilescu M.2004.Removal of heavy metals from the environment by biosorption[J].Engineering in Life Sciences,4(3):219-232
GY Kholodii,et al.2000.Pseudomonas putida partial transposon.[J].Russian Journal of Genetics,36(4):459-469
高圣博,辛嘉英,窦博鑫,等.2017.甲烷氧化菌素催化二价汞还原的研究[J].化学工程师,31(11):1-6
He Z,Gao F,Sha T,et al.2009.Isolation and characterization of a Cr(VI)-reduction Ochrobactrum sp.strain CSCr-3 from chromium landfill[J].Journal of Hazardous Materials,163(2/3):869-873
Jia L,Li B,Xu L,et al.2018.Effects of different preparation conditions on kinetics and adsorption of mercury by biomass char[J].Chinese Journal of Environmental Engineering,12(1):134-144
KadukováJ,Vir c'íkováE.2005.Comparison of differences between copper bioaccumulation and biosorption[J].Environment International,31(2):227-232
Kamnev A A,Ristic'M,Antonyuk L P,et al.1997.Fourier transform infrared spectroscopic study of intact cells of the nitrogen-fixing bacterium Azospirillum brasilense[J].Journal of Molecular Structure,408(408):201-205
Loukidou M X,2005.Zouboulis A I.Biosorption of Toxic Metals[M].Water Encyclopedia.John Wiley&Sons,Inc
梁彩柳.2016.一株海洋类产碱假单胞菌的汞抗性及富集行为机制研究[D].深圳:深圳大学
梁龄予,王耀晶,闫颖,等.2015.玉米芯吸附水中Cr(Ⅵ)的特性及SEM-EDS表征分析[J].生态环境学报,24(2):305-309
刘文静.2017.抗汞菌株的筛选检测以及抗汞蛋白结构与功能的研究[D].合肥:安徽大学
吴鹏宇,夏令.2017.活性污泥胞外聚合物吸附重金属离子的研究进展[J].绿色科技,(16):75-80
Mahbub K R,Krishnan K,Naidu R,et al.2016.Mercury resistance and volatilization by Pseudoxanthomonas,sp.SE1 isolated from soil[J]Environmental Technology&Innovation,6:94-104
Mahbub K R,Krishnan K,Naidu R,et al.2017.Mercury remediation potential of a mercury resistant strain Sphingopyxis sp.SE2 isolated from contaminated soil[J].Journal of Environmental Sciences,51(1):128-137
Majumdar S S,Das S K,Chakravarty R,et al.2010.A study on lead adsorption by Mucor rouxii,biomass[J].Desalination,251(1/3):96-102
Mukhopadhyay M,Noronha S B,Suraishkumar G K.2007.Kinetic modeling for the biosorption of copper by pretreated Aspergillus niger,biomass[J].Bioresource Technology,98(9):1781-1787
Kumar R,Bishnoi N R,Garima,et al.Biosorption of chromium(VI)from aqueous solution and electroplating wastewater using funga biomass[J].Chemical Engineering Journal,135(3):202-208
Osborn A M,Bruce K D,Strike P,et al.1997.Distribution,diversity and evolution of the bacterial mercury resistance(mer)operon.[J]Fems Microbiology Reviews,19(4):239-262
Palinka L A,Sholupov S E,Mashyanov N R,et al.2010.Globa mercury emissions to the atmosphere from anthropogenic and natura sources[J].Atmospheric Chemistry&Physics Discussions,10(13):5951-5964
Qin H,Xiao R,Guo L,et al.2018.Mercury(II)adsorption from aqueous solution using nitrogen and sulfur co-doped activated carbon[J].Water Science and Technology,:wst2018117
Sruthy S,Mohan M,Binish M B.2018.Biosorption of mercury by meta tolerant bacteria from arctic using FT-IR spectroscopy[C].International Conference on Photo-Chemistry and ITS Applications
孙创,宁平,冯权莉,等.2014.含汞排放物处理技术的研究进展[J].现代化工,34(10):49-52
孙阳昭,陈扬,蓝虹,等.2016.中国汞污染的来源、成因及控制技术路径分析[J].环境化学,32(6):937-942
Veglio F,Beolchini F.1997.Removal of metals by biosorption:a review[J].Hydrometallurgy,44(3):301-316
Wei Z S,Wang J B,Huang Z S,et al.2017.Effect of gaseous mercury on nitric oxide removal performance and microbial community of a hybrid catalytic membrane biofilm reactor[J].Chemical Engineering Journal,316:584-591
王建龙,陈灿.2015.重金属生物吸附[M].北京:科学出版社
王佩琦,周伟丽,何圣兵,等.2018.磷对混养反硝化污泥活性和微生物群落结构的影响[J].环境科学,39(3):1350-1356
文晓凤,杜春艳,袁瀚宇,等.2016.改性磁性纳米颗粒固定内生菌Bacillus nealsonii吸附废水中Cd2+的特性研究[J].环境科学学报,36(12):4376-4383
文晓凤,杜春艳,袁瀚宇,等.2016.改性磁性纳米颗粒固定内生菌Bacillus nealsonii吸附废水中Cd2+的特性研究[J].环境科学学报,36(12):4376-4383
吴玉海.2018.工业废水废气治理方法研究[J].信息记录材料,(1):22-23
武振华,孙津生,宋慧平,等.2006.趋磁细菌(MTB)吸附Pd2+的实验研究[J].离子交换与吸附,22(5):385-391
奚涛.2015.重金属汞、铜、铅、镉的酶联免疫吸附快速检测方法研究[D].上海:上海交通大学
熊建林.2016.(NH4)2S法同时脱除冶炼烟气中Hg和SO2[D].昆明:昆明理工大学
Xia L I,Dan Z,Fei S,et al.2017.Biosorption of mercury(Hg2+)from water by immobilized residues from four types of edible mushroom[J].Chinese Journal of Applied&Environmental Biology,23(5):879-885
Yu Z,Li J,Li Y,et al.2014.A mer,operon confers mercury reduction in a Staphylococcus epidermidis,strain isolated from Lanzhou reach of the Yellow River[J].International Biodeterioration&Biodegradation,90(1):57-63
杨天伟,张霁,李涛,等.2016.红外光谱结合化学计量学方法快速鉴别牛肝菌种类及总汞含量分析[J].光谱学与光谱分析,36(11):3510-3516
杨文,陈小敏,朱保虎,等.2017.一株耐汞菌的分离鉴定及其去汞特性[J].环境工程学报,11(1):602-607
杨昕.2016.生物质与煤混合燃烧过程中汞的释放特性研究[D].北京:华北电力大学
姚海燕,李洋,龙飞,等.2016.电子废弃物处理场地重金属污染土壤的钝化修复及其机理初析[J].上海第二工业大学学报,33(2):127-133
Zhang Y,Cui Y,Chen L Y.2012.Mercury inhibits the L170C mutant of aquaporin Z by making waters clog the water channel[J].Biophysical Chemistry,160(1):69-74
Zheng W,Liang L,Gu B.2012.Mercury reduction and oxidation by reduced natural organic matter in anoxic environments[J].Environmental Science&Technology,46(1):292-299
张淑楠,王心义,夏大平.2015.初始p H值对微生物反硝化的影响[J].环境工程,(s1):67-71
张霞忠,袁烈梅.2017.汞吸附研究进展[J].环境污染与防治,39(5):563-568
张志,任洪强,张蓉蓉,等.2005.p H值对好氧颗粒污泥同步硝化反硝化过程的影响[J].中国环境科学,25(6):650-654
赵莉,刘宇,吴洋文,等.2018.燃煤烟气中零价汞的催化氧化理论研究进展[J].中国电力,51(3):170-176