参苓白术散对脾虚水湿不化模型大鼠健脾功效的研究
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摘要
目的:从胃肠功能和免疫功能研究参苓白术散对脾虚水湿不化模型大鼠的健脾功效。方法:采用高脂低蛋白加力竭游泳法复制脾虚水湿不化大鼠模型,检测模型大鼠体质量、血清TP、ALB、尿D-木糖、胃排空率、小肠推进率、免疫器官指数、血清免疫分子水平。结果:给药干预后,与模型对照组比较,参苓白术散组大鼠TP、ALB、尿D-木糖、胃排空率、小肠推进率显著升高(P<0.05,P<0.01);胸腺指数、血清IFN-γ、IL-2、IgG、IgA、C3含量显著升高(P<0.05,P<0.01),IL-4含量显著降低(P<0.01)。结论:参苓白术散对脾虚水湿不化模型大鼠的健脾功效可能与恢复其胃肠功能、Th1/Th2平衡、免疫球蛋白和补体水平有关。
Objective:To explore the efficiency of ShenlingBaizhu Powder on tonifying spleenof model rats with TCM syndrome of dampness stagnancy due to spleen deficiency from gastrointestinal function and immunologic function.Methods:The rats with TCM syndrome of dampness stagnancy due to spleen deficiency were induced by high fat and low protein diet plus swimming exhaustion method. Body mass, serum TP, ALB, urinary D-xylose, gastric empty rate, small intestine propelling rate, immune organ index, immune molecules in serum were taken as indexes to evaluate the effect of shenlingbaizhu powder. Results:Compared with model group, serum protein, urinary D-xylose, gastric empty rate and small intestine propelling ratein ShenlingBaizhu Powder Group were significantly improved(P<0.05, P<0.01). The thymus index,IFN-γ,IL-2,IgG,IgA and C3 in ShenlingBaizhu Powder group were significantly increased(P<0.05, P<0.01), while IL-4 was decreased significantly(P<0.01). Conclusion:The efficiency of ShenlingBaizhu Powder on tonifying spleen of model rats with TCM syndrome of dampness stagnancy due to spleen deficiency may be related to the adjustment of gastrointestinal function, balancing state between Th1 cell and Th2 cell and the improvement the level of immunoglobulin and complement.
Objective:To explore the efficiency of ShenlingBaizhu Powder on tonifying spleenof model rats with TCM syndrome of dampness stagnancy due to spleen deficiency from gastrointestinal function and immunologic function.Methods:The rats with TCM syndrome of dampness stagnancy due to spleen deficiency were induced by high fat and low protein diet plus swimming exhaustion method. Body mass, serum TP, ALB, urinary D-xylose, gastric empty rate, small intestine propelling rate, immune organ index, immune molecules in serum were taken as indexes to evaluate the effect of shenlingbaizhu powder. Results:Compared with model group, serum protein, urinary D-xylose, gastric empty rate and small intestine propelling ratein ShenlingBaizhu Powder Group were significantly improved(P<0.05, P<0.01). The thymus index,IFN-γ,IL-2,IgG,IgA and C3 in ShenlingBaizhu Powder group were significantly increased(P<0.05, P<0.01), while IL-4 was decreased significantly(P<0.01). Conclusion:The efficiency of ShenlingBaizhu Powder on tonifying spleen of model rats with TCM syndrome of dampness stagnancy due to spleen deficiency may be related to the adjustment of gastrointestinal function, balancing state between Th1 cell and Th2 cell and the improvement the level of immunoglobulin and complement.
引文
[1] 赖金枚.参苓白术散对脾胃虚弱型D-IBS患者T淋巴细胞亚群的影响[D].福州:福建中医药大学,2011:34.
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[4] 孙杰,王东晓,桑凯.参苓白术散对COPD大鼠下丘脑及胃组织Ghrelin,Obestatin的影响[J].中国实验方剂学杂志,2018,24(1):85-90.
[5] 楼屹,吴力,冯杨荣,等.参苓白术散联合针刺治疗老年ICU患者肠内营养相关性腹泻的疗效观察[J].中国中医急症,2018,27(1):117-119.
[6] 赵文晓,崔宁,季旭明,等.黄芪及拆分组分对脾虚水湿不化模型大鼠血清学指标的影响[J].时珍国医国药,2015,26(5):1077-1079.
[7] 李慧玉,雷帆,王玉刚,等.甘遂对水负荷小鼠排尿排尿以及肾脏AQP2,IL-1β,TNF-αmRNA表达的影响[J].中国中药杂志,2012,37(5):606-610.
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[9] 刘颖,敬岳,郭丽丽,等. 固本止咳中药对COPD模型小鼠脾、胸腺指数及Th1/Th2失衡的影响[J]. 中华中医药杂志, 2015(2): 504-506.
[10] 吴秀,周联,罗霞,等.四君子汤多糖对脾虚小鼠肠道菌群及免疫功能的影响[J].中药药理与临床,2014,30(2):12-14.
[11] 李云,李伟,张雪廷,等.山药芡实药对不同剂量对脾虚模型小鼠免疫功能的影响[J].北京中医药大学学报,2015,38(8):535-538.
[12] 熊海燕,杨瀚琳,金鑫,等.益生菌复合制剂对脾虚证大鼠胃肠及免疫功能的调节作用[J].现代食品科技,2018,34(5):1-11.
[13] 崔宁,赵文晓,季旭明,等.黄芪及其拆分组分对脾虚水湿不化大鼠肝脏功能的影响[J].中医药信息,2015,32(2):62-65.
[14] 高洁,韩旭,韩冰冰,等.复合建模因素诱导脾虚水湿不化模型大鼠免疫功能的改变[J].中医药信息,2015,32(1):40-42.
[15] 齐明月,邓秀兰,于瀚,等.基于肝郁大鼠模型的肝功能与胃肠功能变化探讨“见肝之病, 知肝传脾 ”的客观性[J].辽宁中医杂志,2017,44(6):1298-1300.
[16] 邵征洋,周红,詹璐,等.调中汤对脾虚肝旺型功能性消化不良幼鼠胃肠动力的影响[J].中华中医药学刊,2017,35(3):526-529.
[17] 姚金晶,陈宜涛.Th1/Th2平衡调节与疾病发生的研究进展[J].现代生物医学进展,2009,9(13):2597-2599.
[18] Bais AG, IBeckmann, Lindemans J, et al.A shift to a peripheral Th2-type cytokine pattern during the carcinogenesis of cervical cancer becomes manifest in CIN Ⅲ lesions[J].Clinical Pathology,2005,58(10):1096-1100.
[19] Yamaguchi T,SomaT,TakakuY,et al.Salbutamol modulates the balance of Th1 and Th2 cytokines by mononuclear cells from allergic asthmatics[J].Int Arch Allergy Immunol,2010,152(Suppl 1):32-40.
[20] Bai A,Lu N,Zeng H,et al.All-trans retinoic acid ameliorates trinitrobenzene sulfonic acid-induced colitis by shifting Th1 to Th2 profile[J].J Interferon Cytokine Res,2010,30(6):399-406.
[21] Huang C,Zhang Z,Wang L,et al.ML-7 attenuates airway inflammation and remodeling via inhibiting the secretion of Th2 cytokines in mice model of asthma[J].Mol Med Rep,2018,17(5):6293-6300.
[2] 韩海荣,宋观礼,胡申.参苓白术散对大黄引起的脾虚泄泻作用机制的研究[J].现代中西医结合杂志,2008,17(1):15-16.
[3] 刘喜平,贾育新,刘勍,等.参苓白术散对溃疡性结肠炎脾虚型模型大鼠CD44、CD54及 CD62p的影响[J].时珍国医国药,2011,22(11):2671-2673.
[4] 孙杰,王东晓,桑凯.参苓白术散对COPD大鼠下丘脑及胃组织Ghrelin,Obestatin的影响[J].中国实验方剂学杂志,2018,24(1):85-90.
[5] 楼屹,吴力,冯杨荣,等.参苓白术散联合针刺治疗老年ICU患者肠内营养相关性腹泻的疗效观察[J].中国中医急症,2018,27(1):117-119.
[6] 赵文晓,崔宁,季旭明,等.黄芪及拆分组分对脾虚水湿不化模型大鼠血清学指标的影响[J].时珍国医国药,2015,26(5):1077-1079.
[7] 李慧玉,雷帆,王玉刚,等.甘遂对水负荷小鼠排尿排尿以及肾脏AQP2,IL-1β,TNF-αmRNA表达的影响[J].中国中药杂志,2012,37(5):606-610.
[8] 崔宁.基于全基因表达谱的黄芪及其拆分组分健脾祛湿机制研究[D].济南:山东中医药大学, 2015: 180.
[9] 刘颖,敬岳,郭丽丽,等. 固本止咳中药对COPD模型小鼠脾、胸腺指数及Th1/Th2失衡的影响[J]. 中华中医药杂志, 2015(2): 504-506.
[10] 吴秀,周联,罗霞,等.四君子汤多糖对脾虚小鼠肠道菌群及免疫功能的影响[J].中药药理与临床,2014,30(2):12-14.
[11] 李云,李伟,张雪廷,等.山药芡实药对不同剂量对脾虚模型小鼠免疫功能的影响[J].北京中医药大学学报,2015,38(8):535-538.
[12] 熊海燕,杨瀚琳,金鑫,等.益生菌复合制剂对脾虚证大鼠胃肠及免疫功能的调节作用[J].现代食品科技,2018,34(5):1-11.
[13] 崔宁,赵文晓,季旭明,等.黄芪及其拆分组分对脾虚水湿不化大鼠肝脏功能的影响[J].中医药信息,2015,32(2):62-65.
[14] 高洁,韩旭,韩冰冰,等.复合建模因素诱导脾虚水湿不化模型大鼠免疫功能的改变[J].中医药信息,2015,32(1):40-42.
[15] 齐明月,邓秀兰,于瀚,等.基于肝郁大鼠模型的肝功能与胃肠功能变化探讨“见肝之病, 知肝传脾 ”的客观性[J].辽宁中医杂志,2017,44(6):1298-1300.
[16] 邵征洋,周红,詹璐,等.调中汤对脾虚肝旺型功能性消化不良幼鼠胃肠动力的影响[J].中华中医药学刊,2017,35(3):526-529.
[17] 姚金晶,陈宜涛.Th1/Th2平衡调节与疾病发生的研究进展[J].现代生物医学进展,2009,9(13):2597-2599.
[18] Bais AG, IBeckmann, Lindemans J, et al.A shift to a peripheral Th2-type cytokine pattern during the carcinogenesis of cervical cancer becomes manifest in CIN Ⅲ lesions[J].Clinical Pathology,2005,58(10):1096-1100.
[19] Yamaguchi T,SomaT,TakakuY,et al.Salbutamol modulates the balance of Th1 and Th2 cytokines by mononuclear cells from allergic asthmatics[J].Int Arch Allergy Immunol,2010,152(Suppl 1):32-40.
[20] Bai A,Lu N,Zeng H,et al.All-trans retinoic acid ameliorates trinitrobenzene sulfonic acid-induced colitis by shifting Th1 to Th2 profile[J].J Interferon Cytokine Res,2010,30(6):399-406.
[21] Huang C,Zhang Z,Wang L,et al.ML-7 attenuates airway inflammation and remodeling via inhibiting the secretion of Th2 cytokines in mice model of asthma[J].Mol Med Rep,2018,17(5):6293-6300.