Fonsecaea monophora色素对人巨噬细胞炎症细胞因子表达的影响
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摘要
目的探究Fonsecaea monophora(F. monophora)色素对THP-1来源的人巨噬细胞炎症细胞因子表达的影响。方法将THP-1人单核细胞诱导为巨噬细胞,分别与F. monophora的标准株、色素株和白化株进行共培养,细胞免疫荧光检测细胞膜表面分子CD14和CD68的表达;定量PCR检测各组中IL-1β、TNF-α、IL-6、IL-10和TGF-β的表达。结果 THP-1人单核细胞诱导为巨噬细胞后,膜表面分子表达由CD14转变为CD68。与F. monophora共培养后,标准株上调IL-6、IL-10和TGF-β的表达(P均<0.05),色素株上调IL-6和TGF-β的表达(P均<0.05),白化株上调IL-1β、TNF-α、IL-6和IL-10的表达(P均<0.05);白化株较标准株能上调IL-1β的表达(P <0.05),标准株较色素株和白化株能上调IL-10和TGF-β的表达(P均<0.05)。结论 F. monophora标准株能促使人巨噬细胞往抗炎状态发展,白化株能促使人巨噬细胞往促炎状态发展,色素株则使人巨噬细胞处于促炎和抗炎的平衡状态,F. monophora色素可能会导致宿主炎症状态紊乱。
Objective To evaluate the effect of Fonsecaea monophora(F. monophora) pigment on the expression of in?ammatory cytokines in THP-1-derived human macrophages. Methods THP-1 human monocytes were induced into macrophages and co-cultured with the standard, pigment and albino strains of F. monophora.The detected by immuno?uorescence. Real-time quantitative PCR was performed to detect the expression of IL-1β,TNF-α, IL-6, IL-10 and TGF-β in each group. Results After the induction of THP-1 human mononuclear cells into macrophages, the expressed membrane surface molecule was changed from CD14 to CD68. After coculture with F. monophora, the expression levels of IL-6, IL-10 and TGF-β were signi?cantly up-regulated in the standard strain(all P < 0.05). The expression of IL-6 and TGF-β was remarkably up-regulated in the pigment strain(both P < 0.05). The expression levels of IL-1β, TNF-α, IL-6 and IL-10 were signi?cantly up-regulated in the albino strain(all P < 0.05). The expression of IL-1β in the albino strain was signi?cantly higher than that in the standard strain(P < 0.05). The expression levels of IL-10 and TGF-β were signi?cantly up-regulated standard Conclusions The standard strain of F.monophora can promote the development of human macrophages into the anti-in?ammatory state. The albino strain can accelerate the progression of human macrophages into the proin?ammatory state, whereas the pigment strain can maintain human macrophages in the balance state between pro-in?ammatory and anti-in?ammatory state, indicating that F. monophora pigment may lead to the disorder of in?ammatory state of the host.
Objective To evaluate the effect of Fonsecaea monophora(F. monophora) pigment on the expression of in?ammatory cytokines in THP-1-derived human macrophages. Methods THP-1 human monocytes were induced into macrophages and co-cultured with the standard, pigment and albino strains of F. monophora.The detected by immuno?uorescence. Real-time quantitative PCR was performed to detect the expression of IL-1β,TNF-α, IL-6, IL-10 and TGF-β in each group. Results After the induction of THP-1 human mononuclear cells into macrophages, the expressed membrane surface molecule was changed from CD14 to CD68. After coculture with F. monophora, the expression levels of IL-6, IL-10 and TGF-β were signi?cantly up-regulated in the standard strain(all P < 0.05). The expression of IL-6 and TGF-β was remarkably up-regulated in the pigment strain(both P < 0.05). The expression levels of IL-1β, TNF-α, IL-6 and IL-10 were signi?cantly up-regulated in the albino strain(all P < 0.05). The expression of IL-1β in the albino strain was signi?cantly higher than that in the standard strain(P < 0.05). The expression levels of IL-10 and TGF-β were signi?cantly up-regulated standard Conclusions The standard strain of F.monophora can promote the development of human macrophages into the anti-in?ammatory state. The albino strain can accelerate the progression of human macrophages into the proin?ammatory state, whereas the pigment strain can maintain human macrophages in the balance state between pro-in?ammatory and anti-in?ammatory state, indicating that F. monophora pigment may lead to the disorder of in?ammatory state of the host.
引文
[1]Queiroz-Telles F, de Hoog S, Santos DW, Salgado CG, Vicente VA, Bonifaz A, Roilides E, Xi L, Azevedo CM, da Silva MB,Pana ZD, Colombo AL, Walsh TJ. Chromoblastomycosis. Clin Microbiol Rev,2017,30(1):233-276.
[2]Lu S, Lu C, Zhang J, Hu Y, Li X, Xi L. Chromoblastomycosis in Mainland China:a systematic review on clinical characteristics. Mycopathologia,2013,175(5-6):489-495.
[3]Fransisca C, He Y, Chen Z, Liu H, Xi L. Molecular identification of chromoblastomycosis clinical isolates in Guang-dong.Med Mycol,2017,55(8):896.
[4]Ameen M. Chromoblastomycosis:clinical presentation and management. Clin Exp Dermatol,2009,34(8):849-854.
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[6]陈少琼,全力,张志刚,邝思驰,陈俊伟,郭月飞.隐球菌性颅内肿块性病变磁共振成像的特征性表现.新医学,2010,41(7):451-454.
[7]Cleinman IB, Gon?alves SS, Nucci M, Quintella DC, Halpern M, Akiti T, Barreiros G, Colombo AL, Santoro-Lopes G. Respiratory tract infection caused by fonsecaea monophora after kidney transplantation. Mycopathologia,2017,182(11-12):11011109.
[8]Verreck FA, de Boer T, Langenberg DM, Hoeve MA, Kramer M, Vaisberg E, Kastelein R, Kolk A, de Waal-Malefyt R,Ottenhoff TH. Human IL-23-producing type 1 macrophages promote but IL-10-producing type 2 macrophages subvert immunity to(myco)bacteria. Proc Natl Acad Sci U S A,2004,101(13):4560-4565.
[9]Martinez FO, Helming L, Gordon S. Alternative activation of macrophages:an immunologic functional perspective. Annu Rev Immunol,2009,27:451-483.
[10]孙佳.褐藻多糖对M2型巨噬细胞CCL2表达的影响与机制研究及外周血粒淋比作为肿瘤预后评价指标的影响因素分析.山东大学,2016.
[11]Gow N, Latge JP, Munro CA. The fungal cell wall:structure,biosynthesis, and function. Microbiol Spectr,2017,5(3).
[12]Mohebbi S, Erfurth F, Hennersdorf P, Brakhage AA, Saluz HP.Hyperspectral imaging using intracellular spies:quantitative real-time measurement of intracellular parameters in vivo during interaction of the pathogenic fungus aspergillus fumigatus with human monocytes. PLoS One,2016,11(10):e163505.
[13]Akoumianaki T, Kyrmizi I, Valsecchi I, Gresnigt MS,Samonis G, Drakos E, Boumpas D, Muszkieta L, Prevost MC,Kontoyiannis DP, Chavakis T, Netea MG, van de Veerdonk FL,Brakhage AA, El-Benna J, Beauvais A, Latge JP, Chamilos G.Aspergillus cell wall melanin blocks LC3-associated phagocytosis to promote pathogenicity. Cell Host Microbe,2016,19(1):79-90.
[14]Zhang J, Wang L, Xi L, Huang H, Hu Y, Li X, Huang X, Lu S,Sun J. Melanin in a meristematic mutant of Fonsecaea monophora inhibits the production of nitric oxide and Th1 cytokines of Murine macrophages. Mycopathologia,2013,175(5-6):515-522.
[15]Jiang Melanization of a meristematic mutant of Fonsecaea monophora increase the pathogenesis in a BALB/c mice infection model.Med Mycol,2018,56(8):979-986.
[2]Lu S, Lu C, Zhang J, Hu Y, Li X, Xi L. Chromoblastomycosis in Mainland China:a systematic review on clinical characteristics. Mycopathologia,2013,175(5-6):489-495.
[3]Fransisca C, He Y, Chen Z, Liu H, Xi L. Molecular identification of chromoblastomycosis clinical isolates in Guang-dong.Med Mycol,2017,55(8):896.
[4]Ameen M. Chromoblastomycosis:clinical presentation and management. Clin Exp Dermatol,2009,34(8):849-854.
[5]Xi L, Lu C, Sun J, Li X, Liu H, Zhang J, Xie Z, De Hoog GS. Chromoblastomycosis caused by a meristematic mutant of Fonsecaea monophora. Med Mycol,2009,47(1):77-80.
[6]陈少琼,全力,张志刚,邝思驰,陈俊伟,郭月飞.隐球菌性颅内肿块性病变磁共振成像的特征性表现.新医学,2010,41(7):451-454.
[7]Cleinman IB, Gon?alves SS, Nucci M, Quintella DC, Halpern M, Akiti T, Barreiros G, Colombo AL, Santoro-Lopes G. Respiratory tract infection caused by fonsecaea monophora after kidney transplantation. Mycopathologia,2017,182(11-12):11011109.
[8]Verreck FA, de Boer T, Langenberg DM, Hoeve MA, Kramer M, Vaisberg E, Kastelein R, Kolk A, de Waal-Malefyt R,Ottenhoff TH. Human IL-23-producing type 1 macrophages promote but IL-10-producing type 2 macrophages subvert immunity to(myco)bacteria. Proc Natl Acad Sci U S A,2004,101(13):4560-4565.
[9]Martinez FO, Helming L, Gordon S. Alternative activation of macrophages:an immunologic functional perspective. Annu Rev Immunol,2009,27:451-483.
[10]孙佳.褐藻多糖对M2型巨噬细胞CCL2表达的影响与机制研究及外周血粒淋比作为肿瘤预后评价指标的影响因素分析.山东大学,2016.
[11]Gow N, Latge JP, Munro CA. The fungal cell wall:structure,biosynthesis, and function. Microbiol Spectr,2017,5(3).
[12]Mohebbi S, Erfurth F, Hennersdorf P, Brakhage AA, Saluz HP.Hyperspectral imaging using intracellular spies:quantitative real-time measurement of intracellular parameters in vivo during interaction of the pathogenic fungus aspergillus fumigatus with human monocytes. PLoS One,2016,11(10):e163505.
[13]Akoumianaki T, Kyrmizi I, Valsecchi I, Gresnigt MS,Samonis G, Drakos E, Boumpas D, Muszkieta L, Prevost MC,Kontoyiannis DP, Chavakis T, Netea MG, van de Veerdonk FL,Brakhage AA, El-Benna J, Beauvais A, Latge JP, Chamilos G.Aspergillus cell wall melanin blocks LC3-associated phagocytosis to promote pathogenicity. Cell Host Microbe,2016,19(1):79-90.
[14]Zhang J, Wang L, Xi L, Huang H, Hu Y, Li X, Huang X, Lu S,Sun J. Melanin in a meristematic mutant of Fonsecaea monophora inhibits the production of nitric oxide and Th1 cytokines of Murine macrophages. Mycopathologia,2013,175(5-6):515-522.
[15]Jiang Melanization of a meristematic mutant of Fonsecaea monophora increase the pathogenesis in a BALB/c mice infection model.Med Mycol,2018,56(8):979-986.