柑橘叶片多酚提取工艺优化

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英文篇名：Optimization of Polyphenol Extraction Conditions in Citrus Leaves 作者：丁萍 ; 刘冰浩 ; 牛英 ; 唐艳 ; 邓光宙 ; 余扣花 英文作者：DING Ping;LIU Bing-hao;NIU Ying;TANG Yan;DENG Guang-zhou;YU Kou-hua;Guangxi Key Laboratory of Citrus Biology,Guangxi academy of specialty crops;Horticulture and Landscape College,Hunan Agricultural University; 关键词：柑橘 ; 叶片 ; 多酚 ; 提取 ; 优化 英文关键词：citrus;;leaves;;polyphenols;;extraction;;optimization 中文刊名：GUXI 英文刊名：Southern Horticulture 机构：广西柑橘生物学重点实验室/广西特色作物研究院;湖南农业大学园艺园林学院; 出版日期：2019-01-20 出版单位：南方园艺 年：2019 期：v.30 基金：广西自然科学青年基金(2013GXNSFBA0);; 广西科技重大专项项目(桂科AA17204026);; 广西重点研发计划项目(桂科AB18221042) 语种：中文; 页：GUXI201901005 页数：5 CN：01 ISSN：45-1368/S 分类号：20-24

摘要

为开展柑橘种质资源抗病性鉴定和评价提供技术支持,采用Folin-Ciocalte法测定柑橘叶片多酚含量,以柑橘叶片多酚提取含量为指标,利用正交试验法分析乙醇浓度、液料比、提取温度、提取时间对提取工艺的影响。结果表明,柑橘叶片多酚的最佳提取工艺参数为液料比50∶1,乙醇浓度30%,提取温度40℃,提取时间30 min。该工艺成本低、易操作,可用于柑橘叶片多酚的测定。
        To provide technical support for the identification and evaluation of disease resistance of citrus germplasm resources, the polyphenol content was measured by Folin-Ciocalte method with the extraction rate of polyphenols from citrus leaves. The effects of ethanol volume fraction, liquid-to-liquid ratio, extraction temperature and extraction time on the yield of polyphenols were evaluated by orthogonal test. The results showed that the optimal extraction process parameters of citrus leaves were 50:1 liquid-to-liquid ratio, 30% ethanol concentration,40° C extraction temperature and 30 min extraction time. The process is low in cost and easy to operate. This process can be used for the determination of polyphenols in citrus leaves.

引文

[1]陈伟,叶明志,周沽.植物酚类物质研究进展[J].福建农业大学学报,1997,26:502,508.
    [2] Hassan M.S.,Behadli A.H.,Alsaadawi I.S. Citrus replant problem in Iraq II possible role of allelopathy[J].plant and Soil,1989(116):157-160.
    [3]李健,杨昌鹏,李群梅,等.植物多酚的研究进展[J].轻工科技,2008,24(12):1-3.
    [4]黄宗安.锥栗疫病的抗病机制与抗病品种选育研究进展[J].林业勘察设计,2008(2):73-76.
    [5]段艳欣,郭文武.多酚含量及多酚氧化酶活性与柑橘胚性愈伤组织褐化的关系[J].中国农学通报,2009,25(15):117-120.