巨尾桉叶、皮、芯中单宁提取及其抗氧化性研究
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摘要
采用加热回流与超声波辅助提取法分别提取了巨尾桉的树叶、树皮、树芯3部位中的单宁,考察了3部位提取物的抗氧化特性。加热回流法提取巨尾桉树叶、树皮、树芯单宁的得率分别为4.19%、1.86%和0.25%。超声波辅助提取法提取巨尾桉树叶、树皮、树芯单宁的得率分别为3.20%、2.25%和0.19%。巨尾桉3部位的单宁提取物对DPPH·、·OH自由基和H2O2均有较好的清除作用,其中,巨尾桉叶、皮、芯单宁提取物对DPPH·自由基清除率分别相当于VC的91.08%、93.20%、79.40%;对·OH自由基清除率分别相当于VC的181%、125%、132%;对H2O2的清除率分别相当于VC的83.60%、65.46%、78.46%。
Conventional refluxing extraction and ultrasound assisted extraction were adopted to extract tannins from leaf,bark and core of Eucalyptus Grandis x E.Urophylla. Moreover the antioxidant activity of tannins extracts also was studied. The yields of tannins from leaf,bark and core by conventional refluxing extraction were 4.19%,1.86% and 0.25% respectively,but that were 3.20%,2.25% and 0.19% respectively by ultrasound assisted extraction. The tannins extracts of Eucalyptus Grandis x E.Urophylla different parts had a strong scavenging activity to DPPH·,·OH free radical and H2O2. The scavenging activity of tannins extracts from leaf,bark and core on DPPH· were the equivalent to VCof 91.08%,93.20% and 79.40%,respectively. And the scavenging activity of 3 tannins extracts on ·OH reached 181%,125% and 132% of VC,on H2O2 equal to83.60%,65.46% and 78.46% VC.
Conventional refluxing extraction and ultrasound assisted extraction were adopted to extract tannins from leaf,bark and core of Eucalyptus Grandis x E.Urophylla. Moreover the antioxidant activity of tannins extracts also was studied. The yields of tannins from leaf,bark and core by conventional refluxing extraction were 4.19%,1.86% and 0.25% respectively,but that were 3.20%,2.25% and 0.19% respectively by ultrasound assisted extraction. The tannins extracts of Eucalyptus Grandis x E.Urophylla different parts had a strong scavenging activity to DPPH·,·OH free radical and H2O2. The scavenging activity of tannins extracts from leaf,bark and core on DPPH· were the equivalent to VCof 91.08%,93.20% and 79.40%,respectively. And the scavenging activity of 3 tannins extracts on ·OH reached 181%,125% and 132% of VC,on H2O2 equal to83.60%,65.46% and 78.46% VC.
引文
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[3]陈海,田宏,肖苏尧,等.桉叶抗氧化物的提取与抗氧化性研究[J].湖南林业科技,2009,36(2):25-29.
[4]黄增,刘雄民,黄丽葵,等.巨尾桉叶中皂苷的提取分离及其抗氧化活性研究[J].天然产物研究与开发,2012,24:1113-1121.
[5]黄增,刘雄民,库咏峰,等.巨尾桉单宁的提取分离及其抗菌抗氧化性能[J].精细化工,2012,29(6):564-583.
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[7]Hou A J,Liu Y Z,Yang H,et al.Hydrolyzable tannins and related polyohenols from Eucalyptus globules[J].Journal of Asian Natural Products Research,2000,2(3):205-212.
[8]陈婷,林红英,张继东,等.G9桉叶抗菌有效部位及活性成分分析[J].中兽医医药杂志,2010,15(2):5-7.
[9]湛年勇,姚创程,陆文海,等.按树皮单宁分析[J].生物质化学工程,2009,43(5):39-41.
[10]王书园,俞凌云,朱云.单宁的分析研究现状[J].江苏调味副食品,2008,25(5):16.
[11]张文华,候旭.单宁抗氧化活性与结构关系的理论研究[M].皮革科学与工程,2009,19(5):9-12.
[12]韩丙军,彭黎旭.植物多酚提取技术及其开发应用现状[J].华南热带农业大学学报,2005,11(1):21-26.
[13]赵峥嵘.银杏叶中单宁提取工艺的优化研究[J].河南农业,2009(7):57-58.
[14]马自超,劳伦斯·波特.桉树叶单宁组分的研究[J].植物学报,1988,30(5):534-538.
[15]Haslam E,Haworth R D,Gramshaw J W,et al.The structure of penta and tetragalloylglucoses and some observations on the molecular weight of gallotannins[J].Chem Soc,1962:2944-2947.
[16]单书香,王本成,李德辉.水解类单宁组份及其结构的研究—Ⅱ橡椀单宁中鞣花酸的分离与鉴定[J].四川林业科技,1980(3):28-34.