基于纳米金/氧化的聚邻苯二胺新颖纳米复合材料修饰电极对尿酸、黄嘌呤、次黄嘌呤的灵敏检测
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摘要
该文采用示差脉冲伏安法、循环伏安法等电化学技术研究了尿酸(UA)、黄嘌呤(XA)、次黄嘌呤(HX)在纳米金/氧化聚邻苯二胺复合材料(Au/p-OPDox/GCE)修饰电极上的电化学行为。在最优的实验条件下,结果显示,该修饰电极对UA、XA和HX均有较好的电催化活性作用,能实现对三种物质的同时测定。UA、XA和HX在该修饰电极上的线性范围分别为3～345、2～113和5～402μmol/L;检测限分别为1.0,1.6和1.5μmol/L。
In this paper, the modified electrode was proposed for the simultaneous determination of uric acid(UA),xanthine(XA) and hypoxanthine(HX) based on oxidized poly(o-phenylenediamine) and gold nanoparticles. Also,cyclic voltammetry(CV) and different pulse voltammetry(DPV) were employed to characterize the modified electrode.Under the optimal conditions, The results showed that the chemically modified electrode displayed excellent electrochemical catalytic activities towards UA,XA and HX. The calibration curves for UA、XA and HX were obtained in the range of 3 ～345、 2 ～113 and 5 ～402 μmol/L with detection limits of 1.0,1.6 and 1.5 μmol/L,respectively.
In this paper, the modified electrode was proposed for the simultaneous determination of uric acid(UA),xanthine(XA) and hypoxanthine(HX) based on oxidized poly(o-phenylenediamine) and gold nanoparticles. Also,cyclic voltammetry(CV) and different pulse voltammetry(DPV) were employed to characterize the modified electrode.Under the optimal conditions, The results showed that the chemically modified electrode displayed excellent electrochemical catalytic activities towards UA,XA and HX. The calibration curves for UA、XA and HX were obtained in the range of 3 ～345、 2 ～113 and 5 ～402 μmol/L with detection limits of 1.0,1.6 and 1.5 μmol/L,respectively.
引文
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[12]Lian Q,He Z,He Q,et al.Simultaneous determination of ascorbic acid,dopamine and uric acid based on tryptophan functionalized graphene[J].Anal.Chim.Acta,2014,120:32-45.
[2]Tanga X,Liu Y,Hou H,et al.A nonenzymatic sensor for xanthine based onelectrospun carbon nanofibers modified electrode[J].Talanta,2011,83:1410-1414.
[3]Westing Y H,Ekblom B,Sj觟din R.The metabolic relation betweenhypoxanthine and uric acid in man following maximal short-distancerunning[J].Acta Physiol Scand.,1989,137:341-345.
[4]Mazzali M,Hughes J.Elevated uric acid increases blood pressure in the rat by a novel crystal-independent mechanism[J].Hypertension,2001,38:1101-1106.
[5]Carpita N C.Measurement of uronic acids without interference from neutral sugars[J].Anal.Biochem.,1991,197:157-162.
[6]Shen G,Yu R.Amperometric glucose biosensor based on electrodeposition of platinum nanoparticles onto covalently immobilized carbon nanotube electrode[J].Talanta,2007,71:1517-1530.
[7]Ferraris S P.Simultaneous determination of insine,hypoxanthine,xanthine,and uric acid and the effect of metal chelators[J].Analy.Biochem.,1991,195:116-121.
[8]Ramaraj R.Mediated reduction of oxygen at poly(phenosafranine)modified electrodes[J].J Appl.Electrochem.,2000,30:757-760.
[9]Li Z,Feng M L,Lu J.KMnO4-Octylphenyl Polygylcol,Ether ChemiluminescenceSyetem for Flow Injection Analysis of Uric Acid in Urine[J].Microchem.J.,1998,59:278-283.
[10]Czauderna M.Dietary selenized yeast and CLA isomer mixture affect fatty and amino acid concentrations in the femoral muscles and liver of rats[J].J Anim.Feed Sci.,2009,18:348-361.
[11]Huo Z H.Sensitive simultaneous determination of catechol and hydroquinone using a gold electrode modified with carbon nanofibers and gold nanoparticles[J].Microchim.Acta,2011,173:119-125.
[12]Lian Q,He Z,He Q,et al.Simultaneous determination of ascorbic acid,dopamine and uric acid based on tryptophan functionalized graphene[J].Anal.Chim.Acta,2014,120:32-45.