正交法制备羧甲基壳聚糖-纳米银纳米微球
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摘要
目的:制备以纳米银为模型药物的羧甲基壳聚糖载药纳米微球,探索载药纳米微球的制备条件对粒径、包封率的影响,确定最佳制备工艺。方法:三聚磷酸钠为交联剂,采用离子交联法制备载药纳米微球,测量药物的包封率及粒径。紫外分光光度法测定该制剂中纳米银释放情况。结果:通过正交实验设计,优化了制备工艺条件,其最佳条件是羧甲基壳聚糖浓度4.2 mg/mL,滴速为5 s/滴,纳米银投药量为20 mg(500μL)。在此条件下进行实验,制备出的载药纳米微球的平均粒径为514.1 nm,多分散系数0.204,包封率61.67%。8 h内累计释放量达80%以上,方程拟合以一级动力学方程拟合效果最好。结论:该制备工艺简单、稳定,可制备出包封率高、粒径适宜的羧甲基壳聚糖-纳米银纳米微球。
Objective: To prepare carboxymethyl chitosan drug-loaded nanoparticles with nano silver as model drug the influence of the drug-loaded nanoparticles preparing conditions on the particle size and the encapsulation efficiency to select the optimum conditions for preparation. Methods:With sodium tripolyphosphate as crosslinking agent, Drug-loaded nanoparticles were prepared by ionic cross-Linking method. The drug encapsulation efficiency and particle size were measured. The release of nano-sliver from preparations byultraviolet spectrophotometry was observed. Results:Through the orthogonal design,preparation method was optimized. The optimum conditions included the carboxymethyl chitosan concentration of 4.2 mg/mL,speed of 5 s/drops,and the best dosage of nano sliver for 20 mg(500 μL). In this experiment,the mean size of the preparation drug-loaded nanoparticles was 514.1 nm with the polydispersity index of0.204,and the encapsulation efficiency was 61.67%.The accumulated amount of release in the preparations is more than 80% at 8 h.Equation of in vitro release model fitted best to Kinetic equation. Conclusion:This preparation method is simple and stable. The carboxymethyl chitosan-nano sliver nanoparticles can be prepared with a high encapsulation efficiency and suitable particle size.
Objective: To prepare carboxymethyl chitosan drug-loaded nanoparticles with nano silver as model drug the influence of the drug-loaded nanoparticles preparing conditions on the particle size and the encapsulation efficiency to select the optimum conditions for preparation. Methods:With sodium tripolyphosphate as crosslinking agent, Drug-loaded nanoparticles were prepared by ionic cross-Linking method. The drug encapsulation efficiency and particle size were measured. The release of nano-sliver from preparations byultraviolet spectrophotometry was observed. Results:Through the orthogonal design,preparation method was optimized. The optimum conditions included the carboxymethyl chitosan concentration of 4.2 mg/mL,speed of 5 s/drops,and the best dosage of nano sliver for 20 mg(500 μL). In this experiment,the mean size of the preparation drug-loaded nanoparticles was 514.1 nm with the polydispersity index of0.204,and the encapsulation efficiency was 61.67%.The accumulated amount of release in the preparations is more than 80% at 8 h.Equation of in vitro release model fitted best to Kinetic equation. Conclusion:This preparation method is simple and stable. The carboxymethyl chitosan-nano sliver nanoparticles can be prepared with a high encapsulation efficiency and suitable particle size.
引文
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[15]赵凡.温度pH敏感微球的制备及对啶虫脒释放性能的研究[D].山东农业大学,2013
[16]李光远,娄建石,陈斌,等.醋甲唑胺纳米球滴眼剂对家兔的降眼压作用及体外释放评价[J].中国新药杂志,2015,24(21):2502
[2]张桂贤,陈斌,王晓辉,等.正交试验优选羧甲基壳聚糖-醋甲唑胺纳米微球的制备方案[J].天津药学,2014,26(2):10
[3]朱寿进,刘法谦,王璟朝,等.新型羧甲基壳聚糖水凝胶的合成与表征[J].高等学校化学学报,2014,35(4):863
[4]Wu J,Zheng Y,Song W,et al.In situ synthesis of silver-nanoparticles/bacterial cellulose composites for slow-released antimicrobial wound dressing[J].Carbohydrate Polymers,2014(102):762
[5]王智康,董政,刘樾,等.液体敷料治疗面部烧伤的临床观察[J].实用临床医药杂志,2013,17(09):72
[6]陈炯,韩春茂,余朝恒.纳米银用于烧伤患者创面后银代谢的变化[J].中华烧伤杂志,2004,20(3):161
[7]孙倩,李明春,马守栋.纳米银抗菌活性及生物安全性研究进展[J].药学研究,2013,32(2):103
[8]蒋卫娟,关静,黄震.载银壳聚糖微球的制备及其抗菌性研究[J].包装工程,2012,33(21):15
[9]李祖浩,王辰宇,王中汉,等.壳聚糖基水凝胶搭载抗菌剂在伤口愈合中的应用[J].中国组织工程研究,2017,21(30):4885
[10]Haider A,Haider S,Kang I K,et al.A novel use of cellulose based filter paper containing silver nanoparticles for its potential application as wound dressing agent[J].2017,(108):455
[11]欧歌,廖德华,张凯,等.采用离子交联法制备壳聚糖胰岛素纳米粒的研究进展[J].中南药学,2013,11(2):116
[12]陈思.还原响应两亲性壳聚糖的制备及其作为纳米载体性能考察[D].广东药科大学,2017
[13]邹竞,肖惠宁,何北海,等.壳聚糖纳米微粒的制备及其载药性能[J].造纸科学与技术,2017,36(2):30
[14]王斌.聚乳酸载柚皮素复合纳米粒的制备、表征及其缓释与抗菌性能[D].太原理工大学,2017
[15]赵凡.温度pH敏感微球的制备及对啶虫脒释放性能的研究[D].山东农业大学,2013
[16]李光远,娄建石,陈斌,等.醋甲唑胺纳米球滴眼剂对家兔的降眼压作用及体外释放评价[J].中国新药杂志,2015,24(21):2502