普通PCR技术和高灵敏HBV-DNA技术检测乙肝病毒DNA的研究
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摘要
目的探讨普通PCR技术和高灵敏PCR在乙肝病毒DNA检测上的临床价值。方法以2016年1月~2017年8月宜春市人民医院收治的43例肝炎患者为研究对象。分别采用普通PCR技术和高灵敏PCR技术进行检测乙型肝炎五项指标,并进行比较。结果普通PCR检测的阳性率是0,高灵敏PCR检测的阳性率是46.5%,阳性率对比差异具有统计学意义(P<0.05);在血清标志物的检测结果组合中,高灵敏PCR检测的"大三阳"、"小三阳"和"小二阳"的阳性率都显著高于普通PCR的检测结果。结论高灵敏HBV-DNA技术在检测乙肝病毒基因DNA上具有更高的准确率,可用于指导临床用药和疗效观察。
Objective To explore the clinical value of common PCR and high sensitive PCR in DNA detection of HBV. Methods 43 patients with hepatitis who were admitted to yichun people's hospital from January 2016 to August 2017 were studied. The five indexes of hepatitis b were detected by common PCR and high sensitive PCR. Results There is no difference between the positive rate and the positive rate of the test. In the combination of the detection results of serum markers, the positive rates of "big three Yang", "small three Yang" and "small two Yang" were significantly higher than that of ordinary PCR. Conclusion High sensitive hbv-dna technology has higher accuracy in detecting the DNA of HBV, which can be used to guide clinical medication and observation of curative effect.
Objective To explore the clinical value of common PCR and high sensitive PCR in DNA detection of HBV. Methods 43 patients with hepatitis who were admitted to yichun people's hospital from January 2016 to August 2017 were studied. The five indexes of hepatitis b were detected by common PCR and high sensitive PCR. Results There is no difference between the positive rate and the positive rate of the test. In the combination of the detection results of serum markers, the positive rates of "big three Yang", "small three Yang" and "small two Yang" were significantly higher than that of ordinary PCR. Conclusion High sensitive hbv-dna technology has higher accuracy in detecting the DNA of HBV, which can be used to guide clinical medication and observation of curative effect.
引文
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[5]朱叶飞,翟祥军,王毓,等.国产ELISA法乙肝表面抗原检测试剂在乙肝流行病学调查中的应用评价[J].江苏预防医学,2013,24(4):9-11.
[6]郭春霞,李嘉,徐曾丽,等.不同HBVDNA检测方法对PEG-IFNa-2b治疗HBe Ag阳性慢性乙型肝炎疗效判断的影响[J].临床肝胆病杂志,2017(4):664-667.
[7]陈继梅,胡娅娴,戴阳.实时荧光定量PCR法检测乙型肝炎病毒YMDD变异的临床意义[J].淮海医药,2017,35(4):398-401.
[8]王庆保,姚玮,邢昕,等.血清学标志物及核酸检测结果在乙型肝炎病毒复制中的相关性研究[J].国际检验医学杂志,2017,38(14):1945-1947.
[9]姚兆基.实时荧光定量PCR法检测乙肝病毒DNA和乙肝病毒标志物的价值[J].内蒙古医学杂志,2017,49(4):485-486.
[10]杜锴.实时荧光定量PCR法检测乙肝病毒DNA的价值分析与研究[J].中国现代药物应用,2017,11(1):55-57.
[2]李强,卓其斌,黄玉仙,等.急性乙型肝炎与慢性乙型肝炎急性发作的临床特征比较[J].临床肝胆病杂志,2016,32(4):706-710.
[3]范文瀚,胡正翠,黄倩芸,等.急性乙型肝炎患者血清学变化过程及临床流行病学特征分析[J].中华肝脏病杂志,2017,25(7):523-525.
[4]许玉苗,沈华芬,李雪芬,等.13039例乙型肝炎患者血清HBV DNA水平流行病学分析[J].中国微生态学杂志,2017,29(4):434-437.
[5]朱叶飞,翟祥军,王毓,等.国产ELISA法乙肝表面抗原检测试剂在乙肝流行病学调查中的应用评价[J].江苏预防医学,2013,24(4):9-11.
[6]郭春霞,李嘉,徐曾丽,等.不同HBVDNA检测方法对PEG-IFNa-2b治疗HBe Ag阳性慢性乙型肝炎疗效判断的影响[J].临床肝胆病杂志,2017(4):664-667.
[7]陈继梅,胡娅娴,戴阳.实时荧光定量PCR法检测乙型肝炎病毒YMDD变异的临床意义[J].淮海医药,2017,35(4):398-401.
[8]王庆保,姚玮,邢昕,等.血清学标志物及核酸检测结果在乙型肝炎病毒复制中的相关性研究[J].国际检验医学杂志,2017,38(14):1945-1947.
[9]姚兆基.实时荧光定量PCR法检测乙肝病毒DNA和乙肝病毒标志物的价值[J].内蒙古医学杂志,2017,49(4):485-486.
[10]杜锴.实时荧光定量PCR法检测乙肝病毒DNA的价值分析与研究[J].中国现代药物应用,2017,11(1):55-57.