摘要
IQM6 (IQ motif containing 6)由At3g58480编码,是拟南芥IQM家族中第6位成员,其基因的功能未见报道。本研究通过筛选与鉴定获得了iqm6-1的纯合体,并利用CRISPR/Cas9基因编辑技术构建iqm6-2;对突变体的表型分析显示, iqm6-1和iqm6-2的远轴面表皮毛的发生晚于野生型Col-0;利用RT-qPCR对表皮毛发生的关键基因进行转录本水平分析发现,与野生型Col-0相比, iqm6-1和iqm6-2延迟miR156的下调和SPL3的上调。这些结果表明,拟南芥IQM6突变可能介导miR156/SPL3组件的表达,推迟远轴面表皮毛的发生。
IQ motif-containing protein 6(IQM6) is coded by At3 g58480, which is the sixth member of the IQM family in Arabidopsis. However, no functional characterizations have been performed for IQM6 in Arabidopsis.In this study, homozygotes of iqm6-1 were identified and iqm6-2 were constructed using CRISPR/Cas9 gene editing technology. The results of phenotypic analysis showed that iqm6-1 and iqm6-2 delayed the initiation of abaxial trichomes. RT-qPCR results showed that iqm6-1 and iqm6-2 delayed the decrease of miRNA156 and the increase of SPL3 transcription level when compared with Col-0. These results suggest that the mutation of IQM6 changes the expression of miRNA156/SPL3, thus delaying the initiation of abaxial trichomes in Arabidopsis.
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