盐碱胁迫下喷施BR对甜菜光系统Ⅱ和氮代谢关键酶的影响
|
摘要
以甜菜主栽品种(KWS0143)为试验材料,共设4个处理,不喷施BR(CK)、叶丛快速生长期喷施BR(Q)、块根膨大期喷施BR(Z)、糖分积累期喷施BR(H),研究盐碱胁迫下不同时期喷施油菜素内酯(Brassinolide,BR)对甜菜叶片光能利用和氮代谢影响。结果表明,叶丛快速生长期喷施BR效果最明显。叶丛快速生长期和糖分积累期喷施BR提高叶片叶绿素含量,块根膨大期喷施BR降低叶绿素含量;各处理在喷施BR后短时间内光能利用率均增强;叶丛快速生长期喷施BR显著提高叶片非光化学淬灭(NPQ)和光保护能力(YNPQ/YNO),块根膨大期和糖分积累期差异不显著;叶丛快速生长期喷施BR显著提高叶片NR和GS活性,块根膨大期喷施BR显著降低NR活性,对GS活性影响不显著,糖分积累期喷施BR显著降低NR活性,提高GS活性;各生长期喷施BR后GOGAT活性均先降后升。
The main sugar beet variety(KWS0143) was used as the test material. There were four treatments, no spraying brassinolide(BR)(CK), spraying BR in the rapid growth period of leaf plexus(Q), spraying BR in the root expansion stage(Z), and spraying BR in the period of sugar accumulation.The effects of spraying BR on the photosynthetic utilization and nitrogen metabolism of sugar beet leaves under different saline-alkali stresses were studied. The results showed that the effect of spraying BR on the rapid growth period of leaf plexus was the most obvious. Spraying BR on the leaf growth stage and sugar accumulation period increased the chlorophyll content of the leaves, and reduced the chlorophyll content in the root enlargement period; the utilization rate of light energy increased in each treatment after spraying BR; the photosynthetic utilization rate of each treatment was enhanced in a short time after spraying BR; spraying BR in the rapid growth period of leaf plexus significantly increased non-photochemical quenching(NPQ) and photoprotective ability(YNPQ/YNO), and the difference between root enlargement and sugar accumulation period was not significant. Spraying BR in the rapid growth period of leaf plexus significantly increased the activity of NR and GS in leaves. Spraying BR in root enlargement significantly reduced NR activity, and had no significant effect on GS activity.Spraying BR in the sugar accumulation period significantly reduced NR activity and increased GS activity. The GOGAT activity decreased first and then increased after spraying BR in each growth period.
The main sugar beet variety(KWS0143) was used as the test material. There were four treatments, no spraying brassinolide(BR)(CK), spraying BR in the rapid growth period of leaf plexus(Q), spraying BR in the root expansion stage(Z), and spraying BR in the period of sugar accumulation.The effects of spraying BR on the photosynthetic utilization and nitrogen metabolism of sugar beet leaves under different saline-alkali stresses were studied. The results showed that the effect of spraying BR on the rapid growth period of leaf plexus was the most obvious. Spraying BR on the leaf growth stage and sugar accumulation period increased the chlorophyll content of the leaves, and reduced the chlorophyll content in the root enlargement period; the utilization rate of light energy increased in each treatment after spraying BR; the photosynthetic utilization rate of each treatment was enhanced in a short time after spraying BR; spraying BR in the rapid growth period of leaf plexus significantly increased non-photochemical quenching(NPQ) and photoprotective ability(YNPQ/YNO), and the difference between root enlargement and sugar accumulation period was not significant. Spraying BR in the rapid growth period of leaf plexus significantly increased the activity of NR and GS in leaves. Spraying BR in root enlargement significantly reduced NR activity, and had no significant effect on GS activity.Spraying BR in the sugar accumulation period significantly reduced NR activity and increased GS activity. The GOGAT activity decreased first and then increased after spraying BR in each growth period.
引文
[1]顾鑫,杨丽,任翠梅,等.基于地统计学的田块尺度盐碱土特性研究[J].中国水土保持,2017(7):48-51.
[2]Munns R,Tester M.Mechanisms of salinity tolerance[J].Annu Rev Plant Biol,2008,59:651-681.
[3]Wang M C,Peng Z Y,Li C L,et al.Proteomic analysis on a high salt tolerance introgression strain of Triticum aestivum/Thinopyrum ponticum[J].Proteomics,2008,8(7):1470-1489.
[4]Porcel R,Aroca R,Ruiz-Lozano J M.Salinity stress alleviation using arbuscular mycorrhizal fungi.A review[J].Agronomy for Sustainable Development,2012,32(1):181-200.
[5]李承业,王燕飞,黄润,等.我国甜菜抗逆性研究进展[J].中国糖料,2001(1):56-58.
[6]耿贵,周建朝,孙立英,等.不同盐度对甜菜生长和养分吸收的影响[J].中国甜菜糖业,2000(1):12-14.
[7]靳开川,何金环.油菜素内酯在植物抗逆中的作用及信号传导机制综述[J].江苏农业科学,2017,45(14):4-7.
[8]寇江涛,师尚礼.2,4-表油菜素内酯对盐胁迫下紫花苜蓿种子萌发及幼苗生长的影响[J].草原与草坪,2015,35(1):1-8,19.
[9]李程,梁宝魁,王晓峰.油菜素内酯提高蔬菜作物抗逆性的研究进展[J].中国蔬菜,2015(11):12-18.
[10]王梦姣,邓百万,杨国鹏.拟南芥油菜素内酯的合成、修饰、信号转导及其在农作物育种中的应用研究进展[J].河南农业科学,2015,44(2):1-6.
[11]李合生,孙群,赵世杰.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[12]Klughammer C,Schreiber U.Complementary PSII quantum yields calculated from simple fluorescence parameters measured by PAM fluorometry and the saturation pulse method[J].PAM Application Notes,2008(1):27-35.
[13]于海滨,蔡葆,孙国琴,等.甜菜硝酸还原酶活性研究[J].中国甜菜,1993(1):18-23.
[14]Zhang C F,Peng S B,Benneti J.Glutamine synthetase and its isoforms in rice spikelets and rachis during grain development[J].Journal of Plant Physiology,2000,156(2):230-233.
[15]Lea P J,Blackwell R D,Chen F L.Enzymes of primary metabolism[M].New York:Academic Press,1990:260-273.
[16]Singh R D,Srivastava H S.Increase in glutamate synthase(NADH)activity in maize seedlings in response to nitrate and ammonium nitrogen[J].Physical Plant,1986,66:413-416.
[17]刘强,王庆成,王占武,等.外源油菜素内酯对苍耳盐碱胁迫的缓解效应[J].东北林业大学学报,2014,42(10):34-37.
[18]刘海英,王曼,文昭普,等.不同时期喷施油菜素内酯对菊花花期和观赏品质的影响[J].湖北农业科学,2014,53(1):97-99.
[19]马野,原换换,裴新涌,等.干旱胁迫下外源油菜素内酯对玉米幼苗光合作用和D1蛋白的调控效应[J].华北农学报,2017,32(3):118-124.
[20]战吉成,王利军,黄卫东.弱光环境下葡萄叶片的生长及其在强光下的光合特性[J].中国农业大学学报,2002(3):75-78.
[21]Klughammer C,Schreiber U.Saturation Pulse method for assessment of energy conversion in PS I[J].PAM Application Notes,2008(1):11-14
[22]黄俊.光逆境下白菜光合特性及抗逆机理的研究[D].南京:南京农业大学,2006.
[23]郝召君,周春华,刘定,等.高温胁迫对芍药光合作用、叶绿素荧光特性及超微结构的影响[J].分子植物育种,2017,15(6):2359-2367.
[24]Shu H M,Guo S Q,Gong Y Y,et al.Effect of brassinolide on salinity tolerance of cotton[J].Agricultural Science&Technology,2014,15(9):1433-1437,1470.
[25]寇江涛.2,4-表油菜素内酯诱导下紫花苜蓿耐盐性生理响应研究[D].兰州:甘肃农业大学,2016.
[26]扈雪欢,宁欢欢,刘光照,等.外源SA对盐胁迫下颠茄生理生化、氮代谢及次生代谢的影响[J].草业学报,2017,26(11):147-156.
[2]Munns R,Tester M.Mechanisms of salinity tolerance[J].Annu Rev Plant Biol,2008,59:651-681.
[3]Wang M C,Peng Z Y,Li C L,et al.Proteomic analysis on a high salt tolerance introgression strain of Triticum aestivum/Thinopyrum ponticum[J].Proteomics,2008,8(7):1470-1489.
[4]Porcel R,Aroca R,Ruiz-Lozano J M.Salinity stress alleviation using arbuscular mycorrhizal fungi.A review[J].Agronomy for Sustainable Development,2012,32(1):181-200.
[5]李承业,王燕飞,黄润,等.我国甜菜抗逆性研究进展[J].中国糖料,2001(1):56-58.
[6]耿贵,周建朝,孙立英,等.不同盐度对甜菜生长和养分吸收的影响[J].中国甜菜糖业,2000(1):12-14.
[7]靳开川,何金环.油菜素内酯在植物抗逆中的作用及信号传导机制综述[J].江苏农业科学,2017,45(14):4-7.
[8]寇江涛,师尚礼.2,4-表油菜素内酯对盐胁迫下紫花苜蓿种子萌发及幼苗生长的影响[J].草原与草坪,2015,35(1):1-8,19.
[9]李程,梁宝魁,王晓峰.油菜素内酯提高蔬菜作物抗逆性的研究进展[J].中国蔬菜,2015(11):12-18.
[10]王梦姣,邓百万,杨国鹏.拟南芥油菜素内酯的合成、修饰、信号转导及其在农作物育种中的应用研究进展[J].河南农业科学,2015,44(2):1-6.
[11]李合生,孙群,赵世杰.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[12]Klughammer C,Schreiber U.Complementary PSII quantum yields calculated from simple fluorescence parameters measured by PAM fluorometry and the saturation pulse method[J].PAM Application Notes,2008(1):27-35.
[13]于海滨,蔡葆,孙国琴,等.甜菜硝酸还原酶活性研究[J].中国甜菜,1993(1):18-23.
[14]Zhang C F,Peng S B,Benneti J.Glutamine synthetase and its isoforms in rice spikelets and rachis during grain development[J].Journal of Plant Physiology,2000,156(2):230-233.
[15]Lea P J,Blackwell R D,Chen F L.Enzymes of primary metabolism[M].New York:Academic Press,1990:260-273.
[16]Singh R D,Srivastava H S.Increase in glutamate synthase(NADH)activity in maize seedlings in response to nitrate and ammonium nitrogen[J].Physical Plant,1986,66:413-416.
[17]刘强,王庆成,王占武,等.外源油菜素内酯对苍耳盐碱胁迫的缓解效应[J].东北林业大学学报,2014,42(10):34-37.
[18]刘海英,王曼,文昭普,等.不同时期喷施油菜素内酯对菊花花期和观赏品质的影响[J].湖北农业科学,2014,53(1):97-99.
[19]马野,原换换,裴新涌,等.干旱胁迫下外源油菜素内酯对玉米幼苗光合作用和D1蛋白的调控效应[J].华北农学报,2017,32(3):118-124.
[20]战吉成,王利军,黄卫东.弱光环境下葡萄叶片的生长及其在强光下的光合特性[J].中国农业大学学报,2002(3):75-78.
[21]Klughammer C,Schreiber U.Saturation Pulse method for assessment of energy conversion in PS I[J].PAM Application Notes,2008(1):11-14
[22]黄俊.光逆境下白菜光合特性及抗逆机理的研究[D].南京:南京农业大学,2006.
[23]郝召君,周春华,刘定,等.高温胁迫对芍药光合作用、叶绿素荧光特性及超微结构的影响[J].分子植物育种,2017,15(6):2359-2367.
[24]Shu H M,Guo S Q,Gong Y Y,et al.Effect of brassinolide on salinity tolerance of cotton[J].Agricultural Science&Technology,2014,15(9):1433-1437,1470.
[25]寇江涛.2,4-表油菜素内酯诱导下紫花苜蓿耐盐性生理响应研究[D].兰州:甘肃农业大学,2016.
[26]扈雪欢,宁欢欢,刘光照,等.外源SA对盐胁迫下颠茄生理生化、氮代谢及次生代谢的影响[J].草业学报,2017,26(11):147-156.