外源喷施油菜素内酯对碱胁迫下甜菜生长的影响
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摘要
为探究碱胁迫下不同质量浓度的油菜素内酯对甜菜生长的影响,在甜菜苗期喷施0.05mg·L-1、0.1mg·L-1、0.15mg·L-1和0.2mg·L-1不同质量浓度的油菜素内酯(BR),分析植株生长特性、光合能力、内源激素、块根产量和含糖率的变化,明确甜菜喷施的最佳质量浓度。结果表明,喷施不同质量浓度BR的处理的光合能力在整个生长期均大于碱处理,并在生长后期显著降低叶片ABA质量浓度,提高CTK和GA3的质量浓度,增加块根产量和产糖量。其中质量浓度为0.15mg·L-1的油菜素内酯处理效果最明显,块根产量最接近CK,达到CK的66.3%,是Alk(碱处理)的157.5%。苗期喷施适宜质量浓度的BR可以缓解碱土对甜菜生长的胁迫,增加干物质积累,增强甜菜的光合作用,降低叶片ABA与其他激素的比例,提高甜菜的抗碱能力。
In order to explore the effects of different mass concentrations of brassinolide on sugarbeet growth under alkali stress.Under alkaline stress,different mass concentrations(0.05 mg·L-1,0.1 mg·L-1,0.15 mg·L-1,0.2 mg·L-1)of brassinolide(BR)were sprayed at the seedling stage of sugar beet.By analyzing the characteristics of plant growth,photosynthetic capacity,endogenous hormones,root tuber yield and sugar content,the optimum mass concentration of sugar beet was investigated.The results showed that spraying different mass concentrations of BR could improve the photosynthetic capacity of sugar beet under alkali stress,decrease the ABA mass concentrations,increase the mass concentrations of CTK and GA3,increase the root yield and sugar content.The results showed that the photosynthetic ability of BR sprayed with different concentration of BR was higher than that of alkali treatment at the whole growth periods,and significantly decreased the ABA concentration of leaves at the late growth period.The root yield and sugar yield increased by increasing the mass concentration of CTK and GA3,and the root yield was the most close to CK when the concentration was0.15 mg·L-1.It reached 66.3% of CK,which was 157.5% of alkali treatment.Spraying BR with appropriate mass concentration at seedling stage could alleviate the stress of alkali soil on sugar beet growth and increase dry matter accumulation.With increase of photosynthesis and sugar beet.The ratio of ABA to other hormones in leaves was low,and the alkali-resistant ability of beet was improved.
In order to explore the effects of different mass concentrations of brassinolide on sugarbeet growth under alkali stress.Under alkaline stress,different mass concentrations(0.05 mg·L-1,0.1 mg·L-1,0.15 mg·L-1,0.2 mg·L-1)of brassinolide(BR)were sprayed at the seedling stage of sugar beet.By analyzing the characteristics of plant growth,photosynthetic capacity,endogenous hormones,root tuber yield and sugar content,the optimum mass concentration of sugar beet was investigated.The results showed that spraying different mass concentrations of BR could improve the photosynthetic capacity of sugar beet under alkali stress,decrease the ABA mass concentrations,increase the mass concentrations of CTK and GA3,increase the root yield and sugar content.The results showed that the photosynthetic ability of BR sprayed with different concentration of BR was higher than that of alkali treatment at the whole growth periods,and significantly decreased the ABA concentration of leaves at the late growth period.The root yield and sugar yield increased by increasing the mass concentration of CTK and GA3,and the root yield was the most close to CK when the concentration was0.15 mg·L-1.It reached 66.3% of CK,which was 157.5% of alkali treatment.Spraying BR with appropriate mass concentration at seedling stage could alleviate the stress of alkali soil on sugar beet growth and increase dry matter accumulation.With increase of photosynthesis and sugar beet.The ratio of ABA to other hormones in leaves was low,and the alkali-resistant ability of beet was improved.
引文
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[11]杨艳君,张谨华,冀瑞萍,等.不同密度、油菜素内酯和2,4-D丁酯对谷子光合特性及产量的影响[J].作物杂志,2015(6):84-90.YANG Y J,ZHANG J H,JI R P,et al.Effects of brassinolide and 2,4-D on photosynthetic characteristics and yield of Zhangzagu 5under different plant densities[J].Crops,2015(6):84-90.
[12]赵雪松,王倩,闫青地,等.油菜素内酯对水稻根系发育的调控作用[J].中国细胞生物学学报,2016,38(10):1191-1198.ZHAO X S,WANG Q,YAN Q D,et al.Function of brassinolide in the regulation of root development in rice[J].Chinese Journal of Cell Biology,2016,38(10):1191-1198.
[13]KUREPIN L V,QADERI M M,BACK T G,et al.A rapid effect of applied brassinolide on abscisic acid concentrations in Brassica napus leaf tissue subjected to short-term heat stress[J].Plant Growth Regulation,2008,55(3):165-167.
[14]李宁,苏晓琼,孙锦,等.外源2,4-表油菜素内酯对弱光胁迫下番茄叶片内源激素和碳水化合物代谢及果实的影响[J].南京农业大学学报,2014,37(3):51-56.LI N,SU X Q,SUN J,et al.Effects of exogenous 2,4-epibrassinolide on endogenous hormone,carbohydrate metabolism and fruits of tomato under low light stress[J].Journal of Nanjing Agricultural University,2014,37(3):51-56.
[2]高淑梅,周继伟.松嫩平原盐碱土现状及改良措施[J].现代化农业,2011,383(6):13-15.GAO SH M,ZHOU J W.Present situation and improvement measures of saline-alkaline soil in Songnen plain[J].Modern Agriculture,2011,383(6):13-15.
[3]李程,梁宝魁,王晓峰.油菜素内酯提高蔬菜作物抗逆性的研究进展[J].中国蔬菜,2015(11):12-18.LI CH,LIANG B K,WANG X F.Research progress on brassinosteroids for improving stress resistance vegetable crops[J].China Vegetables,2015(11):12-18.
[4]刘强,王庆成,王占武,等.外源油菜素内酯对苍耳盐碱胁迫的缓解效应[J].东北林业大学学报,2014,42(10):34-37.LIU Q,WANG Q CH,WANG ZH W,et al.Amelioration of salt-alkaline stress by exogenously applied brassinolide in Xanthium sibiricum[J].Journal of Northeast Forestry University,2014,42(10):34-37.
[5]张睿佳,李瑛,虞秀明,等.高温胁迫与外源油菜素内酯对‘巨峰’葡萄叶片光合生理和果实品质的影响[J].果树学报,2015,32(4):590-596.ZHANG R J,LI Y,YU X M,et al.Effects of heat stress and exogenous brassinolide on photosynthesis ofleaves and berry quality of‘Kyoho’grapevine[J].Journal Fruit Science,2015,32(4):590-596.
[6]菅彩媛.4种植物生长调节剂对甜菜生长及产质量的调控[D].呼和浩特:内蒙古农业大学,2016:9-12.JIAN C Y.Influence of plant growth regulator on growth,yield and quality of sugar beet(Beta vulgaris L.)[D].Hohhot:Inner Mongolia Agricultural University,2016:9-12.
[7]贾洪涛,张立富.油菜素内酯提高小麦萌发期间抗盐性机理初探[J].临沂师范学院学报,2001,23(4):58-59.JIA H T,ZHANG L F.The initial study of salt tolerance mechanism increased by seed soaking of brassinolide during the germination of maize[J].Journal of Linyi Teachers’College,2001,23(4):58-59.
[8]HU Y Q,XIA S T,WANG H Q,et al.Brassinolide increases potato root growth in vitro in a dose-dependent way and alleviates salinity stress[J].Biomed Research International,2016(10):1155.
[9]谢云灿,何孝磊,杜鹏,等.外源油菜素内酯对高温胁迫下大豆光合特性及产量品质的影响[J].大豆科学,2017,36(2):237-243.XIE Y C,HE X L,DU P,et al.Effect of heat acclimation and EBR on photosynthesis characteristics in leaves of soybean flowering stage,yield and quality maturation stage[J].Soybean Science,2017,36(2):237-243.
[10]岳健敏,张金池,尤焱煌,等.油菜素内酯对盐胁迫刺槐苗光合作用及叶绿体超微结构的影响[J].西北农林科技大学学报(自然科学版),2017,45(10):57-66.YUE J M,ZHANG J CH,YOU Y H,et al.Effects of brassinostreoids on photosynthesis and ultrastructure of chloroplasts in Robinia pseudoacacia seedlings under salt stress[J].Journal of Northwest A&F University(Natural Science Edition),2017,45(10):57-66.
[11]杨艳君,张谨华,冀瑞萍,等.不同密度、油菜素内酯和2,4-D丁酯对谷子光合特性及产量的影响[J].作物杂志,2015(6):84-90.YANG Y J,ZHANG J H,JI R P,et al.Effects of brassinolide and 2,4-D on photosynthetic characteristics and yield of Zhangzagu 5under different plant densities[J].Crops,2015(6):84-90.
[12]赵雪松,王倩,闫青地,等.油菜素内酯对水稻根系发育的调控作用[J].中国细胞生物学学报,2016,38(10):1191-1198.ZHAO X S,WANG Q,YAN Q D,et al.Function of brassinolide in the regulation of root development in rice[J].Chinese Journal of Cell Biology,2016,38(10):1191-1198.
[13]KUREPIN L V,QADERI M M,BACK T G,et al.A rapid effect of applied brassinolide on abscisic acid concentrations in Brassica napus leaf tissue subjected to short-term heat stress[J].Plant Growth Regulation,2008,55(3):165-167.
[14]李宁,苏晓琼,孙锦,等.外源2,4-表油菜素内酯对弱光胁迫下番茄叶片内源激素和碳水化合物代谢及果实的影响[J].南京农业大学学报,2014,37(3):51-56.LI N,SU X Q,SUN J,et al.Effects of exogenous 2,4-epibrassinolide on endogenous hormone,carbohydrate metabolism and fruits of tomato under low light stress[J].Journal of Nanjing Agricultural University,2014,37(3):51-56.