基于加速器质谱测量的~(41)Ca植物示踪样品制备方法研究
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摘要
基于加速器质谱测量方法研究~(41)Ca植物示踪样品的制备。~(41)Ca是宇宙成因核素,半衰期较长,且对植物的代谢具有重要的作用。加速器质谱应用长寿命核素,检测方法具有灵敏度高、放射性剂量小、精度高、测量区间大、示踪周期长等优点。离子源引出束流是衡量加速器质谱灵敏度的重要参数,而较高束流强度的引出束流可以提高加速器质谱的灵敏度。为了获得较高的束流强度,本工作系统研究了~(41)Ca植物示踪样品的湿法、干法、以及湿法和干法相结合的二次氟化法,以建立~(41)Ca植物示踪样品制备流程,确定最高束流强度引出时的最优化~(41)Ca植物示踪样品制备参数,为~(41)Ca植物示踪样品广泛应用提供实验基础。
Calcium plays an important role in the metabolism of plants and animals.In this paper,the preparation method of plant ~(41)Ca for accelerator mass spectrometry(AMS)measurement was developed for the first time in China.AMS,with its advantages of high sensitivity,small dose of radioactivity,high accuracy,large measuring range,and long tracer cycle,can be used to measure cosmogenic nuclide ~(41)Ca,which has long half-life.The intensity of the beam in ion source is an important parameter for the sensitivity of AMS measurement.The high beam current can improve the sensitivity of AMS.The preparation methods of plant samples of ~(41)Ca tracer were systematically studied to obtain high beam current using wet,dry and a combining method with wet and dry re-fluoride.A reliable preparation procedure of plant samples for ~(41)Ca tracer and its optimization parameters were determined by testing beam currents of various samples and lay a foundation for the ~(41)Ca-AMS technology at plant tracer applications.
Calcium plays an important role in the metabolism of plants and animals.In this paper,the preparation method of plant ~(41)Ca for accelerator mass spectrometry(AMS)measurement was developed for the first time in China.AMS,with its advantages of high sensitivity,small dose of radioactivity,high accuracy,large measuring range,and long tracer cycle,can be used to measure cosmogenic nuclide ~(41)Ca,which has long half-life.The intensity of the beam in ion source is an important parameter for the sensitivity of AMS measurement.The high beam current can improve the sensitivity of AMS.The preparation methods of plant samples of ~(41)Ca tracer were systematically studied to obtain high beam current using wet,dry and a combining method with wet and dry re-fluoride.A reliable preparation procedure of plant samples for ~(41)Ca tracer and its optimization parameters were determined by testing beam currents of various samples and lay a foundation for the ~(41)Ca-AMS technology at plant tracer applications.
引文
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[2]车玉红.钙肥对红富士苹果果实品质及生理生化特性影响的研究[D].杨凌:西北农林科技大学,2015.
[3]陈见晖,周卫.苹果缺钙对果实钙组分、亚细胞分布与超微结构的影响[J].中国农业科学,2014,37(4):572-576.Chen Jianhui,Zhou Wei.Efect of Calcium Deficiency in Apple(Malus pumila)Fruits on Calcium Fractions,Subcelluar Distribution and Ultrastructure of Pulp Cells[J].Scientia Agricultura Sinica,2014,37(4):572-576(in Chinese).
[4]王玲利,刘超,黄艳花,等.‘黄冠’梨采后热处理和钙处理对其钙形态及细胞壁物质代谢的影响[J].园艺学报,2014,41(2):249-258.Wang Lingli,Liu Chao,Huang Yanhua,et al.Effects of Postharvest Heat and Calcium Treatments on Calcium Fractions and Cell Wall Metabolism of‘Huangguan’Pear Fruit[J].Acta Horticulturae Sinica,2014,41(2):249-258(in Chinese).
[5]Mabuchi H,Takahashi H,Inorg J.The half-life of 41 Ca[J].Jour Inor Nucl Chem,1974,36:1687.
[6]Dong K J,He M,Wu S Y,et al.Application of41 Ca tracer and its AMS measurement in CIAE[J].Journal of Chinese Mass Spectrometry Society,2004,23(4):316-318.
[7]Li S H,Jiang S,He M,et al.Measurements of41 Ca in biological samples by accelerator mass spectrometry using CaF2target[J].High Energ Phys and Nucl Phys,2005,29(12):1 210-1 213.
[8]He M,Ruan X,Wu S,et al.41 Ca analysis using CaF-in CIAE-AMS system[J].Nucl Instr Meth B,2010,268:804-806.
[9]吴绍雷,何明,姜山,等.加速器质谱测量41 Ca方法改进[J].质谱学报,2008(增刊):35-36.Wu Shaolei,He Ming,Jiang Shan,et al,The Improvement of 41 Ca Accelerator Mass Spectrometry Analytical Method[J].Journal of Chinese Mass Spectrometry Society,2008(suppl.):35-36(in Chinese).
[10]窦亮.41 Ca标记内源钙库法测量大鼠钙吸收率的方法研究[D].北京:中国原子能科学研究院,2014.
[11]Zhao X L,Litherland A E,Eliades J,et al.Studies of sputtered anions I:Survey of MFn.[J].Nucl Instrum Methods B,2010,268:807-811.
[12]窦亮,何明,董克君,等.加速器质谱测量岩石样品中41 Ca的初步研究[J].原子能科学技术,2013,47(12):2 322-2 326.Dou Liang,HE Ming,Dong Kejun,et al.Preliminary Study of 41 Ca-AMS Measurement in Rock Samples[J].Atomic Energy Science and Technology,2013,47(12):2 322-2 326(in Chinese).