MiRNA-9在肝癌患者的表达水平及临床意义
摘要
目的探讨miRNA-9在肝细胞癌(HCC)的表达水平和临床意义。方法选取58例HCC患者为观察组,40例健康体检者为对照组。收集研究对象的血液样本,观察组的HCC组织和癌旁组织,采用荧光定量PCR检测血液样本和组织的mi RNA-9的相对表达量;分析miRNA-9与主要临床病理特征的相关性;建立mi RNA-9在HCC中的诊断价值。结果与对照组相比,HCC患者血清中的miRNA-9的相对表达量明显降低;HCC组织的miRNA-9的相对表达量明显低于癌旁组织;低分化、发生转移和TNMⅢ～Ⅳ患者血清和癌组织中的mi RNA-9相对表达量明显低于高中分化、未转移和TNMⅠ～Ⅱ患者;曲线显示mi RNA-9的为81.8%,敏感度和特异度分别为70.0%和90.9%。结论 mi RNA-9可能与HCC的发生和发展相关,为HCC的临床研究和诊断提供新思路。
引文
[1]Trionfini P,Benigni A.MicroRNAs as master regulators of glomerular function in health and disease[J].Journal of the American Society of Nephrology Jasn,2017,28(6):1686-1696.
[2]Kato M,Natarajan R.MicroRNAs in diabetic nephropathy:functions,biomarkers,and therapeutic targets[J].Annals of the New York Academy of Sciences,2015,1353:72.
[3]Liang HH,Wei PL,Hung CS,et al.MicroRNA-200a/b influenced the therapeutic effects of curcumin in hepatocellular carcinoma(HCC)cells[J].Tumor Biology,2013,34(5):3209-3218.
[4]徐华,夏彬,欧阳玉珍,等.微小RNA-9通过TGF R2/Smad2/Smad3信号轴抑制NSCLC增殖、侵袭和迁移[J].江苏医药,2018,44(8):857-862.
[5]刘卓,欧阳胜荣,李媛媛,等.MicroRNA在肿瘤发生发展和诊断中作用的研究进展[J].现代生物医学进展,2016,16(1):163-166.
[6]Wan Y,Cui R,Gu J,et al.Identification of four oxidative stress-responsive micro RNAs,mi R-34a-5p,mi R-1915-3p,mi R-638,and miR-150-3p,in hepatocellular carcinoma[J].Oxidative Medicine and Cellular Longevity,2017(2):5189138.
[7]Yang H,Yu J,Wang L,et al.miR-320a is an independent prognostic biomarker for invasive breast cancer[J].Oncology Letters,2014,8(3):1043-1050.
[8]Serguienko A,Grad I,Wennerstrom AB,et al.Metabolic reprogramming of metastatic breast cancer and melanoma by,let-7a,microRNA[J].Oncotarget,2015,6(4):2451-2465.
[2]Kato M,Natarajan R.MicroRNAs in diabetic nephropathy:functions,biomarkers,and therapeutic targets[J].Annals of the New York Academy of Sciences,2015,1353:72.
[3]Liang HH,Wei PL,Hung CS,et al.MicroRNA-200a/b influenced the therapeutic effects of curcumin in hepatocellular carcinoma(HCC)cells[J].Tumor Biology,2013,34(5):3209-3218.
[4]徐华,夏彬,欧阳玉珍,等.微小RNA-9通过TGF R2/Smad2/Smad3信号轴抑制NSCLC增殖、侵袭和迁移[J].江苏医药,2018,44(8):857-862.
[5]刘卓,欧阳胜荣,李媛媛,等.MicroRNA在肿瘤发生发展和诊断中作用的研究进展[J].现代生物医学进展,2016,16(1):163-166.
[6]Wan Y,Cui R,Gu J,et al.Identification of four oxidative stress-responsive micro RNAs,mi R-34a-5p,mi R-1915-3p,mi R-638,and miR-150-3p,in hepatocellular carcinoma[J].Oxidative Medicine and Cellular Longevity,2017(2):5189138.
[7]Yang H,Yu J,Wang L,et al.miR-320a is an independent prognostic biomarker for invasive breast cancer[J].Oncology Letters,2014,8(3):1043-1050.
[8]Serguienko A,Grad I,Wennerstrom AB,et al.Metabolic reprogramming of metastatic breast cancer and melanoma by,let-7a,microRNA[J].Oncotarget,2015,6(4):2451-2465.